FastQCFastQC Report
Thu 11 Jul 2019
HWC5JBCX2_l01_n02_SM2_581_P1_DM2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWC5JBCX2_l01_n02_SM2_581_P1_DM2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1999567
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTGTAAGGCTTGCATAAATGTTATTTGTTCGAAACTATTCTCTGTCGCT130430.6522912210493572No Hit
ATCCAACACCATGTCAAGCTTTCAGGTAGACTGTTTTCTTTGGCATATTC117080.585526766544957No Hit
CTTATGAGGATGTCAAAAATGCAGTTGGGGTCCTCATCGGAGGACTTGAG110860.5544200319369144No Hit
CCTCATAAGTATGTCCTGGAAGAGAAGGTAATGGTGAAATTTCTCCAACT96190.48105414822309034No Hit
AAACAAGGGTGTTTTTTATCATTAAATAAGCTGAAACGAGAAAGCTCTTA91740.4587993300549569No Hit
CCCTTGGCCTCGACATCAAAACAGCCACTCTTGTTGGGAAACAAATTGTG79130.39573567677402155No Hit
ACTTCAAGCAGTAGTTGTAAGGCTTGCATAAATGTTATTTGTTCGAAACT78020.39018447493882424No Hit
GGATTGGGTGATGCCCCATTCCTTGATCGGCTACGCCGAGATCAAAAGTC69720.34867548824320466No Hit
GCATAAATGTTATTTGTTCGAAACTATTCTCTGTCGCTTTCAATCTATGC69550.3478253041783546No Hit
GTAGAAACAAGGGTGTTTTTTATCATTAAATAAGCTGAAACGAGAAAGCT64250.32131956568597103No Hit
GAAGAGGCAACACCCTTGGCCTCGACATCAAAACAGCCACTCTTGTTGGG60710.3036157328061525No Hit
AGATAATAGGCCCTCTTTGCGTGCGATTGGACCAGGCGGTCATGGATAAG55730.27871034078878076No Hit
CCTTATTTCCTCAAATTTCTGTCCCAATTGTTCCCGCCACTTCTCATTTC54600.2730591172988952No Hit
ATATACAACAGGATGGGAACAGTGACCACAGAAGCTGCTTTTGGTCTAGT54180.27095866255044215No Hit
ACTAAGGGCTTTCACTGAGGAGGGAGCAATAGTTGGAGAAATTTCACCAT52700.26355706010351243No Hit
GTTCTTATCCATGACCGCCTGGTCCAATCGCACGCAAAGAGGGCCTATTA51960.25985625888004754No Hit
CTACTAAGGGCTTTCACTGAGGAGGGAGCAATAGTTGGAGAAATTTCACC51210.2561054468292385No Hit
GCTCTTATCTCTTGCTCTACTTCAAGCAGTAGTTGTAAGGCTTGCATAAA51070.25540529524642086No Hit
ACATAATGGAATCCAACACCATGTCAAGCTTTCAGGTAGACTGTTTTCTT48070.24040204704318485No Hit
GATAAGAACATAGTACTGGAAGCAAACTTCAGTGTAATCTTCAACCGATT47790.2390017438775495No Hit
GTGTTACCATTCCACTCAAGTCCTCCGATGAGGACCCCAACTGCATTTTT47660.23835160312207596No Hit
GAGGAGGGAGCAATAGTTGGAGAAATTTCACCATTACCTTCTCTTCCAGG45020.22514874470322827No Hit
CCTTGATACTACTAAGGGCTTTCACTGAGGAGGGAGCAATAGTTGGAGAA44750.223798452364937No Hit
TCTCATTTCTGCTCTGGAGGTAGTGAAGGTCTCCCATTCTCATCACAGCT43700.2185473154938044No Hit
AAACTATTCTCTGTCGCTTTCAATCTATGCCGTATTTCTTCAATTAACCA43230.21619680660863078No Hit
GGTTCATGCTTATGCCTAGGCAAAAGATAATAGGCCCTCTTTGCGTGCGA43070.21539663337112486No Hit
CTTTCAATCTATGCCGTATTTCTTCAATTAACCACCTTATTTCCTCAAAT42360.21184586462969232No Hit
CTCTTATCTCTTGCTCTACTTCAAGCAGTAGTTGTAAGGCTTGCATAAAT42010.21009548567264813No Hit
GCTCTATGTTGACAAAATGACCATCGTCAACATCCACAGCACTCTGCTGC40790.2039941647366655No Hit
CTCTTGCTCTACTTCAAGCAGTAGTTGTAAGGCTTGCATAAATGTTATTT39710.19859299538350053No Hit
GTATCAAGGTCTCTAATCGGTTGAAGATTACACTGAAGTTTGCTTCCAGT38770.19389197761315324No Hit
GTTTTTTATCATTAAATAAGCTGAAACGAGAAAGCTCTTATCTCTTGCTC38530.19269171775689437No Hit
GTAGTTGTAAGGCTTGCATAAATGTTATTTGTTCGAAACTATTCTCTGTC37710.18859082991467652No Hit
GTACTGGAAGCAAACTTCAGTGTAATCTTCAACCGATTAGAGACCTTGAT37440.18724053757638529No Hit
GTCCCAATAGTTCTCATTGCATGTACCATCTGCCTAGTCTTATTAGCAAC37330.18669041847559997No Hit
GCTCTACTTCAAGCAGTAGTTGTAAGGCTTGCATAAATGTTATTTGTTCG37020.18514008282793226No Hit
ACTATGTTCTTATCCATGACCGCCTGGTCCAATCGCACGCAAAGAGGGCC36390.18198940070525269No Hit
GTACTATGTTCTTATCCATGACCGCCTGGTCCAATCGCACGCAAAGAGGG35890.17948885933804667No Hit
GAACATAGTACTGGAAGCAAACTTCAGTGTAATCTTCAACCGATTAGAGA35330.17668825300677596No Hit
GCTCTGGAGGTAGTGAAGGTCTCCCATTCTCATCACAGCTTCTCCAAGCG34550.17278740847393462No Hit
GGCATAAGCATGAACCAGTCTCGTGACATTTCCTCGAGGGTCATGTCAGA33660.16833644484030794No Hit
CTATTGGGACTCATCCTAGCTCCAGTGCTGGTCTGAGAGATGACCTTCTT33220.16613596843716663No Hit
ATTCTCTGTCGCTTTCAATCTATGCCGTATTTCTTCAATTAACCACCTTA33030.16518576271762836No Hit
ACATAGTACTGGAAGCAAACTTCAGTGTAATCTTCAACCGATTAGAGACC32960.16483568692621953No Hit
CTCTTGTTGGGAAACAAATTGTGGAATGGATTTTGAAAGAGGAATCCAGC32810.16408552451605773No Hit
TTTCTGACATGACCCTCGAGGAAATGTCACGAGACTGGTTCATGCTTATG32770.16388548120668123No Hit
GTTGTATATGAGGCCCATGCAACTGGCAAGTGCACCAGTTGAATAGCTTA32590.16298528631448708No Hit
ATCCATGACCGCCTGGTCCAATCGCACGCAAAGAGGGCCTATTATCTTTT32270.16138493983947524No Hit
CAATGGATTGGGTGATGCCCCATTCCTTGATCGGCTACGCCGAGATCAAA32080.16043473411993697No Hit
GCTTATGCCTAGGCAAAAGATAATAGGCCCTCTTTGCGTGCGATTGGACC31730.15868435516289278No Hit
GTCATGGATAAGAACATAGTACTGGAAGCAAACTTCAGTGTAATCTTCAA31360.15683395455116034No Hit
TGGTAACACGGTTCGAGTCTCTGAAAATATACAGAGATTCGCTTGGAGAA31020.15513358642146025No Hit
TGCTTATGCCTAGGCAAAAGATAATAGGCCCTCTTTGCGTGCGATTGGAC31000.15503356476677202No Hit
GTACCTACTTCGCGTTACATTTCTGACATGACCCTCGAGGAAATGTCACG30460.15233298009018953No Hit
CTCTAGCTCTATGTTGACAAAATGACCATCGTCAACATCCACAGCACTCT30350.15178286098940422No Hit
GGATAGAGCAGTTAAACTATACAAGAAGCTCAAAAGAGAAATAACGTTCC30110.1505826011331453No Hit
CATCTGTACCTACTTCGCGTTACATTTCTGACATGACCCTCGAGGAAATG29400.1470318323917128No Hit
CCCTTAGTAGTATCAAGGTCTCTAATCGGTTGAAGATTACACTGAAGTTT29170.14588158336279805No Hit
GTCTTATTAGCAACCTCCATGGCCTCTGCTGCCTGTTCACTCGATCCAGC28980.14493137764325978No Hit
GATGAGTCTTCTAACCGAGGTCGAAACGTACGTTCTTTCTATCATCCCGT28180.14093051145573016No Hit
CTCCCATTCTCATCACAGCTTCTCCAAGCGAATCTCTGTATATTTTCAGA28140.1407304681463537No Hit
GCCTAGTCTTATTAGCAACCTCCATGGCCTCTGCTGCCTGTTCACTCGAT27800.1390301000166536No Hit
CTTTTCAAACCGTATTTAAAGCGACGATAAATGCATTTGAAAAAAAGACG27580.13792986181508296No Hit
ATCTTCAACCGATTAGAGACCTTGATACTACTAAGGGCTTTCACTGAGGA27450.1372797210596094No Hit
ATTAGAGACCTTGATACTACTAAGGGCTTTCACTGAGGAGGGAGCAATAG27320.13662958030413586No Hit
TCACTGAGGAGGGAGCAATAGTTGGAGAAATTTCACCATTACCTTCTCTT27140.13572938541194168No Hit
TCTCTGTATATTTTCAGAGACTCGAACCGTGTTACCATTCCACTCAAGTC27120.13562936375725343No Hit
CTGTATATTTTCAGAGACTCGAACCGTGTTACCATTCCACTCAAGTCCTC27080.13542932044787698No Hit
GCATGAACCAGTCTCGTGACATTTCCTCGAGGGTCATGTCAGAAATGTAA26990.1349792230017799No Hit
CTCATGGACTCAGGCACTCCTTCCGTAGAAGGCCCTCTTTTCAAACCGTA26880.13442910390099458No Hit
CTTATCTCTTGCTCTACTTCAAGCAGTAGTTGTAAGGCTTGCATAAATGT26740.13372895231817689No Hit
CATCACAGCTTCTCCAAGCGAATCTCTGTATATTTTCAGAGACTCGAACC26710.13357891983614453No Hit
GTTTTCTTTGGCATATTCGCAAGCGATTTGCAGACAATGGATTGGGTGAT26620.13312882239004745No Hit
GTGCTAGCCAGCACCATTCTGTTTTCATGCCTGATTAGTGGATTGGTGGT26490.13247868163457388No Hit
GTATATTTTCAGAGACTCGAACCGTGTTACCATTCCACTCAAGTCCTCCG26160.13082832433221792No Hit
GTTTTTTACTCTAGCTCTATGTTGACAAAATGACCATCGTCAACATCCAC26130.13067829185018556No Hit
TGATACTACTAAGGGCTTTCACTGAGGAGGGAGCAATAGTTGGAGAAATT25970.12987811861267964No Hit
GTCCCAATTGTTCCCGCCACTTCTCATTTCTGCTCTGGAGGTAGTGAAGG25940.1297280861306473No Hit
GTAATCCACAATATCAGGTGCAAGATCCCAATGATGTTTGCTGCAATGAC25320.12662741483531184No Hit
AGCGAAAGCAGGGTGACAAAAACATAATGGAATCCAACACCATGTCAAGC25150.1257772307704618No Hit
CTTCCAGGACATACTTATGAGGATGTCAAAAATGCAGTTGGGGTCCTCAT25060.12532713332436474No Hit
CAGTAGTTGTAAGGCTTGCATAAATGTTATTTGTTCGAAACTATTCTCTG25020.12512709001498826No Hit
CTATTGCTCCCTCCTCAGTGAAAGCCCTTAGTAGTATCAAGGTCTCTAAT24920.12462698174154706No Hit
TCCTAGCTCCAGTGCTGGTCTGAGAGATGACCTTCTTGAAAATTTGCAGG24510.12257653782043812No Hit
CTCATGGAATGGCTAAAGACAAGACCAATCTTGTCACCTCTGACTAAGGG24380.12192639706496457No Hit
GTGTAATCTTCAACCGATTAGAGACCTTGATACTACTAAGGGCTTTCACT23950.1197759314891674No Hit
AAAGCAGGGTGACAAAAACATAATGGAATCCAACACCATGTCAAGCTTTC23880.11942585569775857No Hit
ATTTAAAGATGAGTCTTCTAACCGAGGTCGAAACGTACGTTCTTTCTATC23840.11922581238838208No Hit
ATGCCGTATTTCTTCAATTAACCACCTTATTTCCTCAAATTTCTGTCCCA23760.11882572576962913No Hit
TCATTAAATAAGCTGAAACGAGAAAGCTCTTATCTCTTGCTCTACTTCAA23750.11877571494228499No Hit
CACTTAGAATGACAATTGCATCTGTACCTACTTCGCGTTACATTTCTGAC23580.11792553087743496No Hit
CGATTGGACCAGGCGGTCATGGATAAGAACATAGTACTGGAAGCAAACTT23370.11687530350320845No Hit
GTTCGAAACTATTCTCTGTCGCTTTCAATCTATGCCGTATTTCTTCAATT23240.1162251627477349No Hit
GATAATAGGCCCTCTTTGCGTGCGATTGGACCAGGCGGTCATGGATAAGA23230.11617515192039077No Hit
CTTGTGAACAGATTGCTGATTCACAGCATCGGTCTCACAGACAAATGGCT23150.11577506530163781No Hit
CTGCTGCTCCTGTTGATATTCTTCCCTCATGGACTCAGGCACTCCTTCCG23130.11567504364694955No Hit
GTTCATGCTTATGCCTAGGCAAAAGATAATAGGCCCTCTTTGCGTGCGAT23120.11562503281960544No Hit
GTTGATATTCTTCCCTCATGGACTCAGGCACTCCTTCCGTAGAAGGCCCT23110.11557502199226131No Hit
GTTGAAGATTACACTGAAGTTTGCTTCCAGTACTATGTTCTTATCCATGA22760.11382464303521714No Hit
GTAGTATCAAGGTCTCTAATCGGTTGAAGATTACACTGAAGTTTGCTTCC22730.11367461055318477No Hit
GTTCCATAGCCTTTGCCGTAGTGCTAGCCAGCACCATTCTGTTTTCATGC22670.11337454558912005No Hit
GGACATACTTATGAGGATGTCAAAAATGCAGTTGGGGTCCTCATCGGAGG22650.11327452393443181No Hit
GTCCAATCGCACGCAAAGAGGGCCTATTATCTTTTGCCTAGGCATAAGCA22510.11257437235161413No Hit
AGTATGTCCTGGAAGAGAAGGTAATGGTGAAATTTCTCCAACTATTGCTC22480.11242433986958177No Hit
ATACAAGAAGCTCAAAAGAGAAATAACGTTCCATGGGGCCAAGGAGGTGT22300.11152414497738761No Hit
GTATTTCTTCAATTAACCACCTTATTTCCTCAAATTTCTGTCCCAATTGT22290.11147413415004348No Hit
CCTTAAAAGGAAGAGGCAACACCCTTGGCCTCGACATCAAAACAGCCACT22250.111274090840667No Hit
ATGTCAAGCTTTCAGGTAGACTGTTTTCTTTGGCATATTCGCAAGCGATT21720.10862351699142865No Hit
TCCATAGCCTTTGCCGTAGTGCTAGCCAGCACCATTCTGTTTTCATGCCT21700.10852349533674041No Hit
TGTTATTTGTTCGAAACTATTCTCTGTCGCTTTCAATCTATGCCGTATTT21670.10837346285470806No Hit
CATCAAAACAGCCACTCTTGTTGGGAAACAAATTGTGGAATGGATTTTGA21440.10722321382579329No Hit
GGCTAGCACTACGGCAAAGGCTATGGAACAGGTGGCTGGATCGAGTGAAC21400.1070231705164168No Hit
GTACAGATGCAATTGTCATTCTAAGTGTCTCGCTGGATTCCTCTTTCAAA21390.1069731596890727No Hit
GGTAACACGGTTCGAGTCTCTGAAAATATACAGAGATTCGCTTGGAGAAG21070.10537281321406085No Hit
GAGCAATAGTTGGAGAAATTTCACCATTACCTTCTCTTCCAGGACATACT21020.10512275907734026No Hit
CCTCAGTGAAAGCCCTTAGTAGTATCAAGGTCTCTAATCGGTTGAAGATT20870.10437259666717844No Hit
TCACTAAGCTATTCAACTGGTGCACTTGCCAGTTGCATGGGCCTCATATA20760.10382247756639312No Hit
GATCAAAAGTCCTTAAAAGGAAGAGGCAACACCCTTGGCCTCGACATCAA20760.10382247756639312No Hit
GTTCACAGCAGTGGGTAAAGAGTTCAACCACCTGGAAAAAAGAATAGAGA20580.10292228267419898No Hit
GCATGTACCATCTGCCTAGTCTTATTAGCAACCTCCATGGCCTCTGCTGC20560.10282226101951072No Hit
CAGGTAGACTGTTTTCTTTGGCATATTCGCAAGCGATTTGCAGACAATGG20500.10252219605544599No Hit
CCACAGCACTCTGCTGCTCCTGTTGATATTCTTCCCTCATGGACTCAGGC20500.10252219605544599No Hit
GACTCGAACCGTGTTACCATTCCACTCAAGTCCTCCGATGAGGACCCCAA20430.10217212026403717No Hit
CCGTGCCCAGTGAGCGAGGACTGCAGCGTAGACGCTTTATCCAAAATGCC20270.10137194702653125No Hit
TTCCAGTACTATGTTCTTATCCATGACCGCCTGGTCCAATCGCACGCAAA20230.10117190371715476No Hit
GTCTTCTAACCGAGGTCGAAACGTACGTTCTTTCTATCATCCCGTCAGGC20220.10112189288981063No Hit
CTGTACCTACTTCGCGTTACATTTCTGACATGACCCTCGAGGAAATGTCA20100.10052176296168121No Hit
GCACTGGAGCTAGGATGAGTCCCAATAGTTCTCATTGCATGTACCATCTG20020.10012167634292823No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGATCC202.1219057E-6145.12648145
GAGCGAA1450.0129.88241
CCTCGCC1150.0113.57725145
GGCTGCC203.8532398E-4108.84486145
CTCACCC352.7484566E-7103.66177145
GGTGTCC301.5929405E-596.750984145
TGACGCC950.091.65882145
ACCTCCC1350.091.37593145
CAGGTAC1150.088.181023
GCTGGCC750.087.07589145
ATTCGCC750.087.07589145
CGGACCC608.240022E-1084.65711145
GTACTGA1200.084.523726
GTAGAAA19050.083.271061
ACCGCCC702.910383E-1182.92942145
GGTCTAA353.4232813E-582.92942145
AGTAGAA7900.082.520221
CGAGTCC1850.082.36909145
TTCTACT1950.081.720864
GCCGGCC651.5552359E-978.14503145
Produced by FastQC (version 0.11.5)