FastQCFastQC Report
Tue 2 Jul 2019
HWC5JBCX2_l01_n02_SM2_510_P1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWC5JBCX2_l01_n02_SM2_510_P1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1369748
Sequences flagged as poor quality0
Sequence length151
%GC39

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGCACGCACTTTCTTAAAATGTCGCTGTTTGGAGACACAATTGCCTACCT79350.5793036383334744No Hit
GTAAGAAATACAGTAAAAATTGAATTTAATGCGATTCTTCTACCTCCAAA66250.48366560856449514No Hit
GATATACAGAAAGCACTAATTGGTGCCTCTATCTGCTTTTTAAAACCCAA52380.3824061068167283No Hit
CTATAGAACATGCTTGTAAATTCAAACATTCCAGTTTCGTTACAGTAACT51710.3775146961338874No Hit
ATATAGTAGCGCTGAGCTTTCATGGCCTTCTGCTATTTCAAATGCTTCAT47490.346706109444949No Hit
CTACTATATTGTCTCATGGTCATGTACCTGAATCCTGGAAATTATTCAAT43660.31874476181020156No Hit
CATTATAGGTATTTCTTCACAAGAGCTGAATTTCCCATAGAGCTCTGCTT38480.28092758668017764No Hit
GTGTGAATGTGATGCTTGTTTTTCGCACAAAGCACAGAGCGTTCCTAGTT35240.25727360069151406No Hit
GTAGTAACAAGAGCATTTTTCAATAACGTTTCTTTGTAATGATGACAAGC33400.24384047284610016No Hit
ACTATATTGTCTCATGGTCATGTACCTGAATCCTGGAAATTATTCAATGC32640.23829200699690745No Hit
ATACTATACTGCTCTATTACTCAACTGCTGCTTCTAGTTTGGCTGTAACA32000.23361961470285045No Hit
GTTCTATAGAGATGGATTTGTATCTAACTTTGCAATGGAAATTCCTTCAT31960.23332759018447188No Hit
ATATTGTCTCATGGTCATGTACCTGAATCCTGGAAATTATTCAATGCAAG31210.22785213046487385No Hit
GCTTTAGCACTTCCATTACATCCTTTGCAATTCCTTCCCCATTCTTCTGA31040.22661102626176494No Hit
GCCTTGGAATGGATAAAAAACAAAAGATGCTTAACTGATATACAGAAAGC30740.22442084237392573No Hit
GTTCATAGCTGAGACCATCTGCATTTCCCGTCTCACTCCTGGCACTGAGG29990.21894538265432767No Hit
GAATAAAGGGGCCAAATAAAGAGACAATAAACAGAGAGGTATCAATTTTG27930.20390611995783164No Hit
CATTCACACAGAGCTCATAGCAGAGCAGCGAGATCCTCAGTGCCAGGAGT26360.19244415761147307No Hit
GCTATGAGCTCTGTGTGAATGTGATGCTTGTTTTTCGCACAAAGCACAGA25820.18850182661336246No Hit
CCTATAATGTTTGAACCATTCCAGATTCTTTCAATTTGTTCTTTTATTTT24360.1778429316925449No Hit
GTCCTATTGTCCAAGCCATGAAATGGAGAGCTGATAAAATAAAAGAACAA24120.17609078458227354No Hit
AGCAGGCACTTTCTTAAAATGTCGCTGTTTGGAGACACAATTGCCTACCT23790.1736815823056504No Hit
TTATTATTGTCATTCCTATTGCCATATCTGCTGATTCATTTACTCCAGCA23060.1683521348452416No Hit
ATTACTATGTGGTCACTCAATACTTCCATGTTGTCAGAGAGTACTTCCTT22950.16754906741970055No Hit
CTTCTGACTTGCCCCAAGGGATCTCAATACTCCAATGTTGCTTTGCAGTT22810.16652698160537557No Hit
GTCATGTACCTGAATCCTGGAAATTATTCAATGCAAGTGAAACTAGGAAC22120.16148955866334538No Hit
GCTCATAGCAGAGCAGCGAGATCCTCAGTGCCAGGAGTGAGACGGGAAAT20890.15250980472320458No Hit
ATTACATCCTTTGCAATTCCTTCCCCATTCTTCTGACTTGCCCCAAGGGA20260.14791041855874218No Hit
GCTATGAACACAGCAAAAACAATGAATGGGATGGGAAAAGGAGAAGACGT19990.14593925305968689No Hit
TTCTAATGGTATGCTGAACAGATCAGGACAAGGTATTTGAGCCTTTAGTC19410.14170489754319773No Hit
GTCTCATGGTCATGTACCTGAATCCTGGAAATTATTCAATGCAAGTGAAA19200.14017176882171026No Hit
GTATAGTATGGTTATCCAATCCATCATCATTTAAAGATGCAGCAGTAATG19170.13995275043292635No Hit
GTAGTAACAAGAGGATTTTTCAATAACGTTTCTTTGTAATGATGACAAGC19140.13973373204414244No Hit
GCACTAATTGGTGCCTCTATCTGCTTTTTAAAACCCAAAGACCAGGAAAG18980.13856563397062815No Hit
CTCCAATGTTGCTTTGCAGTTCTTCTGCCAGTTTTTGGACGTCTTCTCCT18710.1365944684715729No Hit
CAATTACTATGTGGTCACTCAATACTTCCATGTTGTCAGAGAGTACTTCC18150.132506125214273No Hit
GTTTAATATGCTATCTACCGTGTTGGGAGTAGCAGCACTAGGCATCAAAA18030.1316300516591373No Hit
GTACTTCCTTCATTGCTTCTTTAGCCTGAATTTCTTTTTGGTAACTGTGT18000.1314110332703534No Hit
CTGATATACAGAAAGCACTAATTGGTGCCTCTATCTGCTTTTTAAAACCC17860.1303889474560284No Hit
GTTTATTGTCTCTTTATTTGGCCCCTTTATTCTTATTTTCATGTTTACTC17850.13031594132643376No Hit
CTATTGTCCAAGCCATGAAATGGAGAGCTGATAAAATAAAAGAACAAATT17140.12513250612521426No Hit
GACCTAGACTCTGCCTTGGAATGGATAAAAAACAAAAGATGCTTAACTGA16940.12367238353332147No Hit
AAATTACACTGTTGGTTCGGTGGGAAAGAATTTGACCTAGACTCTGCCTT16520.12060612609034656No Hit
GTTCTTTATTATTGTCATTCCTATTGCCATATCTGCTGATTCATTTACTC16060.11724784412899306No Hit
GCACAGAGCGTTCCTAGTTTCACTTGCATTGAATAATTTCCAGGATTCAG15940.11637177057385738No Hit
ATTCATCACAGAGCCCTTATCGGGAATGGGAACAACAGCAACAAAAAAGA15780.11520367250034313No Hit
GTCCAAGCCATGAAATGGAGAGCTGATAAAATAAAAGAACAAATTGAAAG15750.11498465411155921No Hit
AGTAGAAACAACGCACTTTTTCCAGTTTATTTGCTGACATTGATTACAAT15550.11352453151966639No Hit
AAGAAATACAGTAAAAATTGAATTTAATGCGATTCTTCTACCTCCAAAAC15390.11235643344615215No Hit
CAATACTCCAATGTTGCTTTGCAGTTCTTCTGCCAGTTTTTGGACGTCTT15110.1103122618175022No Hit
GTTCAAACATTATAGGTATTTCTTCACAAGAGCTGAATTTCCCATAGAGC14750.10768404115209514No Hit
GGTTCAAACATTATAGGTATTTCTTCACAAGAGCTGAATTTCCCATAGAG14320.10454477757952559No Hit
GTATAGATCTGTTCCTTTTGGTTCTCCAACTATGAGTTCCAGACACTGCA14290.10432575919074166No Hit
CCATTACATCCTTTGCAATTCCTTCCCCATTCTTCTGACTTGCCCCAAGG13910.10155152626614529No Hit
GTATTTACACAGTCTCTCCAAAGTTTCCATAGGAAATTTTCGTTAGCTTT13810.10082146497019889No Hit
ATACTAAATGGAATGAATGCTTAAATCCACGAATTTTTTTGGCTATGACT13700.10001839754465786No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGCTCCG100.007108946144.878467
TGACGCC500.0130.60042145
AGCACGC10850.0129.517961
CACGCAC11000.0127.75183
GCACGCA11050.0127.173742
CGCACCC301.096887E-7120.92631145
CGGTGCA256.4939195E-6115.8985442
ACCCCCC203.854554E-4108.833664145
GCGAGAA750.0106.415146145
GAAACAC3350.0103.789744
CGCACTT14050.0101.5658955
ACGCACT14150.0100.336194
CGACGAA950.099.286865145
AAACACG3600.096.582125
TCCAGGG554.129106E-1092.34373145
GCCGACC406.0907405E-790.69472145
GGCACCC259.3588524E-487.06694145
AGGCCCC259.3588524E-487.06694145
GAACGCC259.3588524E-487.06694145
TAGCTAA502.257184E-887.06694145
Produced by FastQC (version 0.11.5)