FastQCFastQC Report
Tue 2 Jul 2019
HWC5JBCX2_l01_n02_SM2_463_P1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWC5JBCX2_l01_n02_SM2_463_P1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1899393
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATAATATGTAGATTGATTTTATTACCCATATAACTCTTAACTCTGCTG76410.40228641465984133No Hit
ATATACAACAGGATGGGAACAGTGACCACAGAAGCTGCTTTTGGTCTAGT73650.3877554566116649No Hit
CTCTATGACCGTCTGAAAGGCCATTTGCGTTCGTTTTACGGCTACTTTGC73420.38654454344098355No Hit
ATATAAGGCAGCCATCGGGAGCGCTAAAACCTCCACCTCCTAAAATTGCA66730.3513227646937732No Hit
GTTGTATATGAGGCCCATGCAACTGGCAAGTGCACCAGTTGAATAGCTTA52600.27693057729495685No Hit
GATAAGGACCCTTTAGGGTGCGTAAGCACGCTCGACTAATAAGGAGAGGG51570.2715077922262533No Hit
GCTCTATGTTGACAAAATGACCATCGTCAACATCCACAGCACTCTGCTGC50710.2669800299358795No Hit
GCCTTATATTGGGCCCCCTCTCCTCTCCCTCTTGGGTTTACTTATTGCCC48900.25745066976660436No Hit
GTTCCAATAGTTCTCATTGCATGTACCATCTGCCTAGTCTTATTAGCAAC43510.2290731828536801No Hit
GTTAAGGAGAGGGAGAGGGAGAGGACCTTTAGGTGCTCGACTGTTAAGGA43390.22844140206897678No Hit
GTCTTATTAGCAACCTCCATGGCCTCAGCTGCCTGTTCACTCGATCCAGC43210.22749373089192176No Hit
CTCATGGAATGGCTAAAGACAAGACCAATCTTGTCACCTCTGACTAAGGG42300.2227027266079216No Hit
GGATAGAGCAGTTAAACTATACAAGAAGCTCAAAAGAGAAATAACGTTCC40840.21501606039403112No Hit
CCATTATATATGTCATTTCTCTCAATGAAATGAAAGAGAAAGCTTCCGAA39240.20659231659798682No Hit
GCCTAGTCTTATTAGCAACCTCCATGGCCTCAGCTGCCTGTTCACTCGAT39060.20564464542093186No Hit
CTCTAGCTCTATGTTGACAAAATGACCATCGTCAACATCCACAGCACTCT37990.2000112667573272No Hit
CTCATGGACTCAGGCACTCCTTCCGTAGAAGGCCCTCTTTTCAAACCGTA37750.19874770518792054No Hit
GTGCTAGCCAGCACCATTCTGTTTTCATGCCTGATTAGTGGATTGGTGGT36060.18985012580334876No Hit
ATTTAAAGATGAGTCTTCTAACCGAGGTCGAAACGTACGTTCTTTCTATC35790.18842861903776628No Hit
AATAAGGAGAGGGGGCTCGACTGATAAGGACCTTTAGGTGCTCGACCCCA35450.18663857348110688No Hit
CTATTGGAACTCATCCTAGCTCCAGTGCTGGTCTGAGAGATGACCTTCTT34300.18058400762770002No Hit
TCCATAGCCTTTGCCGTAGTGCTAGCCAGCACCATTCTGTTTTCATGCCT33860.17826747808378782No Hit
CATATAGACTGTTGAACACAGATTTTGCCAGTAAGGTTCTGCACACTTTT33140.17447679337556787No Hit
AATAAGGAGAGGGGGGCTCGACTGATAAGGAGAGGAGAGGACCTTTAGGT32830.1728446930150843No Hit
CTGCTGCTCCTGTTGATATTCTTCCCTCATGGACTCAGGCACTCCTTCCG32660.1719496702367546No Hit
CTTTTCAAACCGTATTTAAAGCGACGATAAATGCATTTGAAAAAAAGACG32580.17152848304695237No Hit
ATATATGTCATTTCTCTCAATGAAATGAAAGAGAAAGCTTCCGAAGCACA32240.16973843749029296No Hit
GTTTTTTACTCTAGCTCTATGTTGACAAAATGACCATCGTCAACATCCAC32150.16926460190176545No Hit
ATATAGTGGTATTCCTTCCCCCTCGTGACTCGGGTCTTCAATGCTTAATT32050.16873811791451268No Hit
GTAATCCACAATATCAGGTGCAAGATCCCAATGATGTTTGCTGCAATGAC32020.16858017271833686No Hit
ATACAAGAAGCTCAAAAGAGAAATAACGTTCCATGGGGCCAAGGAGGTGT31710.16694807235785328No Hit
GCACTGGAGCTAGGATGAGTTCCAATAGTTCTCATTGCATGTACCATCTG31510.16589510438334773No Hit
CCCTTTAGGGTGCGTAAGCACGCTCGACTAATAAGGAGAGGGGGCTCGAC30710.16168323248532557No Hit
GTTCCATAGCCTTTGCCGTAGTGCTAGCCAGCACCATTCTGTTTTCATGC30490.1605249677133695No Hit
ATATAACTCTTAACTCTGCTGACTTGATATCCCCATTATATATGTCATTT30260.1593140545426881No Hit
GTCTTCTAACCGAGGTCGAAACGTACGTTCTTTCTATCATCCCGTCAGGC29880.1573134153911276No Hit
CCTTTAGGTGCTCGACCCCAGGTTTACTTATTGGGTGGGGCAATAAGTAA29870.15726076699240232No Hit
CCACTATATGATGCAATCAAATGCATGAAGACATTCTTTGGCTGGAAAGA29840.15710282179622648No Hit
GTTGATATTCTTCCCTCATGGACTCAGGCACTCCTTCCGTAGAAGGCCCT29790.1568395798026001No Hit
TCACTAAGCTATTCAACTGGTGCACTTGCCAGTTGCATGGGCCTCATATA29560.1556286666319187No Hit
ATAATATGTAGATTGATTTTATTACCCATATAACTCTTAACTCTGCTGAC29520.1554180730370176No Hit
CCCTTTAGGGTGCGTAAGCACGCTCGACTAATAAGGAGAGGGGGGCTCGA29060.15299624669565487No Hit
ATGTTATTATTGACCAATAAGATGGCGGAGGGTTTCACAGCTTTCCCTCC27890.14683638404479749No Hit
GCATGTACCATCTGCCTAGTCTTATTAGCAACCTCCATGGCCTCAGCTGC27320.1438354253174567No Hit
CTATACAAGAAGCTCAAAAGAGAAATAACGTTCCATGGGGCCAAGGAGGT27320.1438354253174567No Hit
GCTAAAGACAAGACCAATCTTGTCACCTCTGACTAAGGGAATTTTAGGAT27270.14357218332383032No Hit
TTCTTGATATATGTTATTATTGACCAATAAGATGGCGGAGGGTTTCACAG27090.14262451214677532No Hit
CCACAGCACTCTGCTGCTCCTGTTGATATTCTTCCCTCATGGACTCAGGC27080.14257186374805003No Hit
TGTTATTATTGACCAATAAGATGGCGGAGGGTTTCACAGCTTTCCCTCCT26850.14136095057736867No Hit
GGCTAAAGACAAGACCAATCTTGTCACCTCTGACTAAGGGAATTTTAGGA26170.13778085946404983No Hit
CTTCTAACCGAGGTCGAAACGTACGTTCTTTCTATCATCCCGTCAGGCCC26100.1374123206729729No Hit
CTCTTAACTCTGCTGACTTGATATCCCCATTATATATGTCATTTCTCTCA25940.13656994629336847No Hit
ACTCTTAACTCTGCTGACTTGATATCCCCATTATATATGTCATTTCTCTC25900.13635935269846736No Hit
GATAAGGACCTTTAGGTGCTCGACCCCAGGTTTACTTATTGGGTGGGGCA25860.13614875910356625No Hit
GGCTAGCACTACGGCAAAGGCTATGGAACAGGTGGCTGGATCGAGTGAAC25490.134200768350731No Hit
ATATTGAGGACTGCGATAGGTCAAGTGTCGAGGCCCATGTTCCTATATGT25430.13388487795837933No Hit
GGCTATGGAACAGGTGGCTGGATCGAGTGAACAGGCAGCTGAGGCCATGG25180.1325686679902474No Hit
AGCCAGCACCATTCTGTTTTCATGCCTGATTAGTGGATTGGTGGTAGTAG24670.12988359965525828No Hit
CCACAATATCAGGTGCAAGATCCCAATGATGTTTGCTGCAATGACGAGAG24610.12956770926290662No Hit
CTATCATCCCGTCAGGCCCCCTCAAAGCCGAGATCGCACAGAGACTGGAA24570.1293571156680055No Hit
CCTCAATATCATGTCTCCTATTTCAAGAACACAGTATTTTTCCCATTTGT24340.12814620249732414No Hit
GCACTCTGCTGCTCCTGTTGATATTCTTCCCTCATGGACTCAGGCACTCC24300.12793560890242303No Hit
CACTTGAACCGCTGCATCTGCACTCCCATTCGCTTCTGGTAGGCCTGCAA23680.12467140818145588No Hit
CTTGTGAACAGATTGCTGATTCACAGCATCGGTCTCACAGACAAATGGCT23420.12330254981459868No Hit
GGGCCAAGGAGGTGTCACTAAGCTATTCAACTGGTGCACTTGCCAGTTGC23270.1225128238337195No Hit
GATCTTGAGGCTCTCATGGAATGGCTAAAGACAAGACCAATCTTGTCACC23040.12130191066303815No Hit
CCCATTCGCTTCTGGTAGGCCTGCAAATTTTCAAGAAGGTCATCTCTCAG22920.12067012987833482No Hit
GTTCCCATCCTGTTGTATATGAGGCCCATGCAACTGGCAAGTGCACCAGT22870.12040688788470842No Hit
GTGCTGGCTAGCACTACGGCAAAGGCTATGGAACAGGTGGCTGGATCGAG22860.12035423948598316No Hit
CTTTCTATCATCCCGTCAGGCCCCCTCAAAGCCGAGATCGCACAGAGACT22690.11945921670765344No Hit
ATAATAACATATATCAAGAATGACCAACGGGAGTACGCAGGGACCAACCC22670.11935391991020289No Hit
ATATTGGGCCCCCTCTCCTCTCCCTCTTGGGTTTACTTATTGCCCCACCC22540.1186694907267743No Hit
CCCTTATGGTCGAGCACCTAAAGGTCCTCTCCTCTCCTTATCAGTCGAGC22380.11782711634716987No Hit
AGCTCTATGTTGACAAAATGACCATCGTCAACATCCACAGCACTCTGCTG21630.11387848644277408No Hit
GGTAGATATTTAAAGATGAGTCTTCTAACCGAGGTCGAAACGTACGTTCT21550.11345729925297188No Hit
GTATATGTAACACAACAGGGATAGAGAAGCCTAAATTTCTTCCTGATTTG21440.11287816686699381No Hit
CCTAAAGGTTAGGGTTTACTTATTGGGTTGGGGGGTTAACAGTCGAGCAT21350.11240433127846633No Hit
AAACAAGGTAGTTTTTTACTCTAGCTCTATGTTGACAAAATGACCATCGT21090.11103547291160913No Hit
GCTCTATCCATGTTGTTCGGGTCCCCATTTCCATTTAGGGCATTTTGGAT20870.10987720813965304No Hit
CTCCAGTGCTGGTCTGAGAGATGACCTTCTTGAAAATTTGCAGGCCTACC20860.10982455974092775No Hit
GTATTTAAAGCGACGATAAATGCATTTGAAAAAAAGACGATCAGTAATCC20470.10777127219064196No Hit
GATAAGGAGAGGAGAGGACCTTTAGGTGCTCGACCATAAGGGAGAGGATG20340.10708684300721336No Hit
GTTAAGGATGCGTAAGCAGGCTCGACTGATAAGGACCCTTTAGGGTGCGT20290.10682360101358698No Hit
GGTTTACTTATTGGGTGGGGCAATAAGTAAACCCAAGAGGAGAGGACCTT20060.10561268784290559No Hit
CCTCTGACTAAGGGAATTTTAGGATTTGTGTTCACGCTCACCGTGCCCAG20020.1054020942480045No Hit
TCTCAGACCAGCACTGGAGCTAGGATGAGTTCCAATAGTTCTCATTGCAT19800.10424382947604839No Hit
GTAGAAGGCCCTCTTTTCAAACCGTATTTAAAGCGACGATAAATGCATTT19730.10387529068497146No Hit
CAGTTAAACTATACAAGAAGCTCAAAAGAGAAATAACGTTCCATGGGGCC19650.10345410349516926No Hit
TCCTAGCTCCAGTGCTGGTCTGAGAGATGACCTTCTTGAAAATTTGCAGG19570.10303291630536704No Hit
ACCATAAGGGAGAGGATGCGCAAGCATGCTCGACTGTTAACCCCCCAACC19380.10203259672958677No Hit
AGCAGGTACTGATCCAAAATGGAAGACTTTGTGCGACAATGCTTCAATCC19360.10192729993213621No Hit
CTCATATACAACAGGATGGGAACAGTGACCACAGAAGCTGCTTTTGGTCT19310.10166405793850984No Hit
GTATATGAGGCCCATGCAACTGGCAAGTGCACCAGTTGAATAGCTTAGTG19290.10155876114105929No Hit
ATACAACAGGATGGGAACAGTGACCACAGAAGCTGCTTTTGGTCTAGTTT19280.101506112742334No Hit
CCTTTAGGGTGCGTAAGCACGCTCGACTAATAAGGAGAGGGGGCTCGACT19050.10029519957165263No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTCC501.8189894E-12116.10304145
AGTAGAA4800.0102.5993041
GCCACCC1100.098.951454145
CAGGTAC3450.096.564063
GTACTGA3500.095.1945956
TGACGCC850.085.36988145
TCACGCC1200.084.65846145
CGAAAGC6050.079.006953
ACGATTT651.553417E-978.14627145
GCGAAAG6250.077.6375052
GGTACTG4300.077.4778445
CCTGTTT1650.074.76332145
CTATAAA1000.072.5644145
TTAGTCC406.635474E-572.5644145
CCGCACC903.6379788E-1272.5644145
CGCTCGC702.7939677E-972.56439145
GTAGAAA6900.071.373432
CAAGCCC1250.069.66183145
GAAAGCA7350.065.0329364
AGGTACT5400.063.034874
Produced by FastQC (version 0.11.5)