FastQCFastQC Report
Thu 11 Jul 2019
HWC5JBCX2_l01_n02_SM2_463_P1_DM2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWC5JBCX2_l01_n02_SM2_463_P1_DM2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1905961
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATAATATGTAGATTGATTTTATTACCCATATAACTCTTAACTCTGCTG76580.4017920618522625No Hit
ATATACAACAGGATGGGAACAGTGACCACAGAAGCTGCTTTTGGTCTAGT73780.38710131004779214No Hit
CTCTATGACCGTCTGAAAGGCCATTTGCGTTCGTTTTACGGCTACTTTGC73510.3856847018380754No Hit
ATATAAGGCAGCCATCGGGAGCGCTAAAACCTCCACCTCCTAAAATTGCA66840.3506892323609979No Hit
GTTGTATATGAGGCCCATGCAACTGGCAAGTGCACCAGTTGAATAGCTTA52680.27639600180696244No Hit
GATAAGGACCCTTTAGGGTGCGTAAGCACGCTCGACTAATAAGGAGAGGG51680.2711493047339374No Hit
GCTCTATGTTGACAAAATGACCATCGTCAACATCCACAGCACTCTGCTGC50810.26658467828040555No Hit
GCCTTATATTGGGCCCCCTCTCCTCTCCCTCTTGGGTTTACTTATTGCCC48930.2567208877831183No Hit
GTTCCAATAGTTCTCATTGCATGTACCATCTGCCTAGTCTTATTAGCAAC43590.22870352541316424No Hit
GTTAAGGAGAGGGAGAGGGAGAGGACCTTTAGGTGCTCGACTGTTAAGGA43560.2285461245009735No Hit
GTCTTATTAGCAACCTCCATGGCCTCAGCTGCCTGTTCACTCGATCCAGC43330.22733938417417776No Hit
CTCATGGAATGGCTAAAGACAAGACCAATCTTGTCACCTCTGACTAAGGG42390.22240748892553416No Hit
GGATAGAGCAGTTAAACTATACAAGAAGCTCAAAAGAGAAATAACGTTCC40910.214642377257457No Hit
CCATTATATATGTCATTTCTCTCAATGAAATGAAAGAGAAAGCTTCCGAA39290.2061427279991563No Hit
GCCTAGTCTTATTAGCAACCTCCATGGCCTCAGCTGCCTGTTCACTCGAT39170.20551312435039332No Hit
CTCTAGCTCTATGTTGACAAAATGACCATCGTCAACATCCACAGCACTCT38080.19979422454079596No Hit
CTCATGGACTCAGGCACTCCTTCCGTAGAAGGCCCTCTTTTCAAACCGTA37850.19858748421400016No Hit
GTGCTAGCCAGCACCATTCTGTTTTCATGCCTGATTAGTGGATTGGTGGT36120.18951069827766676No Hit
ATTTAAAGATGAGTCTTCTAACCGAGGTCGAAACGTACGTTCTTTCTATC35860.18814655703868022No Hit
AATAAGGAGAGGGGGCTCGACTGATAAGGACCTTTAGGTGCTCGACCCCA35500.18625774609239118No Hit
CTATTGGAACTCATCCTAGCTCCAGTGCTGGTCTGAGAGATGACCTTCTT34380.18038144537060308No Hit
TCCATAGCCTTTGCCGTAGTGCTAGCCAGCACCATTCTGTTTTCATGCCT33940.17807289865847203No Hit
CATATAGACTGTTGAACACAGATTTTGCCAGTAAGGTTCTGCACACTTTT33220.17429527676589396No Hit
AATAAGGAGAGGGGGGCTCGACTGATAAGGAGAGGAGAGGACCTTTAGGT32900.1726163337025259No Hit
CTGCTGCTCCTGTTGATATTCTTCCCTCATGGACTCAGGCACTCCTTCCG32780.1719867300537629No Hit
CTTTTCAAACCGTATTTAAAGCGACGATAAATGCATTTGAAAAAAAGACG32670.17140959337573014No Hit
ATATATGTCATTTCTCTCAATGAAATGAAAGAGAAAGCTTCCGAAGCACA32320.16957324940017135No Hit
GTTTTTTACTCTAGCTCTATGTTGACAAAATGACCATCGTCAACATCCAC32230.1691010466635991No Hit
GTAATCCACAATATCAGGTGCAAGATCCCAATGATGTTTGCTGCAATGAC32110.16847144301483608No Hit
ATATAGTGGTATTCCTTCCCCCTCGTGACTCGGGTCTTCAATGCTTAATT32100.16841897604410586No Hit
ATACAAGAAGCTCAAAAGAGAAATAACGTTCCATGGGGCCAAGGAGGTGT31800.1668449669221983No Hit
GCACTGGAGCTAGGATGAGTTCCAATAGTTCTCATTGCATGTACCATCTG31590.16574316053686303No Hit
CCCTTTAGGGTGCGTAAGCACGCTCGACTAATAAGGAGAGGGGGCTCGAC30800.16159826984917322No Hit
GTTCCATAGCCTTTGCCGTAGTGCTAGCCAGCACCATTCTGTTTTCATGC30530.1601816616394564No Hit
ATATAACTCTTAACTCTGCTGACTTGATATCCCCATTATATATGTCATTT30310.15902738828339089No Hit
CCTTTAGGTGCTCGACCCCAGGTTTACTTATTGGGTGGGGCAATAAGTAA29980.15729597824929262No Hit
GTCTTCTAACCGAGGTCGAAACGTACGTTCTTTCTATCATCCCGTCAGGC29920.1569811764249111No Hit
CCACTATATGATGCAATCAAATGCATGAAGACATTCTTTGGCTGGAAAGA29890.15682377551272034No Hit
GTTGATATTCTTCCCTCATGGACTCAGGCACTCCTTCCGTAGAAGGCCCT29840.1565614406590691No Hit
TCACTAAGCTATTCAACTGGTGCACTTGCCAGTTGCATGGGCCTCATATA29610.15535470033227333No Hit
ATAATATGTAGATTGATTTTATTACCCATATAACTCTTAACTCTGCTGAC29580.1551972994200826No Hit
CCCTTTAGGGTGCGTAAGCACGCTCGACTAATAAGGAGAGGGGGGCTCGA29120.15278381876649103No Hit
ATGTTATTATTGACCAATAAGATGGCGGAGGGTTTCACAGCTTTCCCTCC27910.14643531530813067No Hit
GCATGTACCATCTGCCTAGTCTTATTAGCAACCTCCATGGCCTCAGCTGC27370.1436020988886971No Hit
CTATACAAGAAGCTCAAAAGAGAAATAACGTTCCATGGGGCCAAGGAGGT27330.14339223100577608No Hit
GCTAAAGACAAGACCAATCTTGTCACCTCTGACTAAGGGAATTTTAGGAT27330.14339223100577608No Hit
CCACAGCACTCTGCTGCTCCTGTTGATATTCTTCCCTCATGGACTCAGGC27180.14260522644482232No Hit
TTCTTGATATATGTTATTATTGACCAATAAGATGGCGGAGGGTTTCACAG27160.14250029250336183No Hit
TGTTATTATTGACCAATAAGATGGCGGAGGGTTTCACAGCTTTCCCTCCT26870.14097875035218455No Hit
GGCTAAAGACAAGACCAATCTTGTCACCTCTGACTAAGGGAATTTTAGGA26220.13756839725471823No Hit
CTTCTAACCGAGGTCGAAACGTACGTTCTTTCTATCATCCCGTCAGGCCC26140.13714866148887622No Hit
CTCTTAACTCTGCTGACTTGATATCCCCATTATATATGTCATTTCTCTCA26020.1365190578401132No Hit
ACTCTTAACTCTGCTGACTTGATATCCCCATTATATATGTCATTTCTCTC25960.1362042560157317No Hit
GATAAGGACCTTTAGGTGCTCGACCCCAGGTTTACTTATTGGGTGGGGCA25880.13578452024988968No Hit
GGCTAGCACTACGGCAAAGGCTATGGAACAGGTGGCTGGATCGAGTGAAC25570.1341580441572519No Hit
ATATTGAGGACTGCGATAGGTCAAGTGTCGAGGCCCATGTTCCTATATGT25470.1336333744499494No Hit
GGCTATGGAACAGGTGGCTGGATCGAGTGAACAGGCAGCTGAGGCCATGG25220.13232170018169312No Hit
AGCCAGCACCATTCTGTTTTCATGCCTGATTAGTGGATTGGTGGTAGTAG24710.12964588467445032No Hit
CCACAATATCAGGTGCAAGATCCCAATGATGTTTGCTGCAATGACGAGAG24650.1293310828500688No Hit
CTATCATCCCGTCAGGCCCCCTCAAAGCCGAGATCGCACAGAGACTGGAA24620.12917368193787807No Hit
GCACTCTGCTGCTCCTGTTGATATTCTTCCCTCATGGACTCAGGCACTCC24370.12786200766962177No Hit
CCTCAATATCATGTCTCCTATTTCAAGAACACAGTATTTTTCCCATTTGT24370.12786200766962177No Hit
CACTTGAACCGCTGCATCTGCACTCCCATTCGCTTCTGGTAGGCCTGCAA23750.12460905548434623No Hit
CTTGTGAACAGATTGCTGATTCACAGCATCGGTCTCACAGACAAATGGCT23470.12313998030389918No Hit
GGGCCAAGGAGGTGTCACTAAGCTATTCAACTGGTGCACTTGCCAGTTGC23320.12235297574294542No Hit
GATCTTGAGGCTCTCATGGAATGGCTAAAGACAAGACCAATCTTGTCACC23070.12104130147468914No Hit
CCCATTCGCTTCTGGTAGGCCTGCAAATTTTCAAGAAGGTCATCTCTCAG22980.1205690987381169No Hit
GTGCTGGCTAGCACTACGGCAAAGGCTATGGAACAGGTGGCTGGATCGAG22910.12020182994300513No Hit
GTTCCCATCCTGTTGTATATGAGGCCCATGCAACTGGCAAGTGCACCAGT22870.11999196206008414No Hit
CTTTCTATCATCCCGTCAGGCCCCCTCAAAGCCGAGATCGCACAGAGACT22730.11925742446986061No Hit
ATAATAACATATATCAAGAATGACCAACGGGAGTACGCAGGGACCAACCC22700.11910002355766985No Hit
ATATTGGGCCCCCTCTCCTCTCCCTCTTGGGTTTACTTATTGCCCCACCC22570.11841795293817658No Hit
CCCTTATGGTCGAGCACCTAAAGGTCCTCTCCTCTCCTTATCAGTCGAGC22440.11773588231868333No Hit
AGCTCTATGTTGACAAAATGACCATCGTCAACATCCACAGCACTCTGCTG21680.11374839254318427No Hit
GGTAGATATTTAAAGATGAGTCTTCTAACCGAGGTCGAAACGTACGTTCT21580.11322372283588175No Hit
GTATATGTAACACAACAGGGATAGAGAAGCCTAAATTTCTTCCTGATTTG21480.11269905312857922No Hit
CCTAAAGGTTAGGGTTTACTTATTGGGTTGGGGGGTTAACAGTCGAGCAT21400.11227931736273722No Hit
AAACAAGGTAGTTTTTTACTCTAGCTCTATGTTGACAAAATGACCATCGT21120.1108102421822902No Hit
GCTCTATCCATGTTGTTCGGGTCCCCATTTCCATTTAGGGCATTTTGGAT20900.10965596882622466No Hit
CTCCAGTGCTGGTCTGAGAGATGACCTTCTTGAAAATTTGCAGGCCTACC20880.10955103488476416No Hit
GTATTTAAAGCGACGATAAATGCATTTGAAAAAAAGACGATCAGTAATCC20520.10766222393847513No Hit
GATAAGGAGAGGAGAGGACCTTTAGGTGCTCGACCATAAGGGAGAGGATG20370.10687521937752135No Hit
GTTAAGGATGCGTAAGCAGGCTCGACTGATAAGGACCCTTTAGGGTGCGT20320.1066128845238701No Hit
GGTTTACTTATTGGGTGGGGCAATAAGTAAACCCAAGAGGAGAGGACCTT20080.10535367722634408No Hit
CCTCTGACTAAGGGAATTTTAGGATTTGTGTTCACGCTCACCGTGCCCAG20040.10514380934342309No Hit
TCTCAGACCAGCACTGGAGCTAGGATGAGTTCCAATAGTTCTCATTGCAT19800.10388460204589706No Hit
GTAGAAGGCCCTCTTTTCAAACCGTATTTAAAGCGACGATAAATGCATTT19730.1035173332507853No Hit
CAGTTAAACTATACAAGAAGCTCAAAAGAGAAATAACGTTCCATGGGGCC19670.1032025314264038No Hit
TCCTAGCTCCAGTGCTGGTCTGAGAGATGACCTTCTTGAAAATTTGCAGG19600.10283526263129203No Hit
ACCATAAGGGAGAGGATGCGCAAGCATGCTCGACTGTTAACCCCCCAACC19450.10204825807033828No Hit
AGCAGGTACTGATCCAAAATGGAAGACTTTGTGCGACAATGCTTCAATCC19420.10189085715814751No Hit
CTCATATACAACAGGATGGGAACAGTGACCACAGAAGCTGCTTTTGGTCT19370.10162852230449627No Hit
ATACAACAGGATGGGAACAGTGACCACAGAAGCTGCTTTTGGTCTAGTTT19340.1014711213923055No Hit
GTATATGAGGCCCATGCAACTGGCAAGTGCACCAGTTGAATAGCTTAGTG19290.10120878653865424No Hit
CCTTTAGGGTGCGTAAGCACGCTCGACTAATAAGGAGAGGGGGCTCGACT19110.10026438106550974No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTAGAA4800.0119.236531
TCACGCC1150.0113.55995145
CCGTCGG301.6041335E-596.614429
CAGGTAC3200.090.561763
TAGGGCC651.6370905E-1189.295145
CGGTGCC259.3612616E-487.06263145
TAGTCGC259.3612616E-487.06263145
GTACTGA3550.081.645986
GTGGTCC801.8189894E-1281.621216145
GGTACTG3650.079.400785
AGCGTAA554.3746695E-879.14784145
AACCGCC851.8189894E-1276.81997145
GATTAGA1850.074.407492
GTAGAAA7700.074.331212
ACCGGCC300.001930492872.55219145
GCTACGG702.7994247E-972.55219145
GGACACC300.001930492872.55219145
TAGATAC2100.069.003055
CAAGCCC851.6916601E-1068.28442145
CGAAAGC5650.067.9613953
Produced by FastQC (version 0.11.5)