FastQCFastQC Report
Tue 2 Jul 2019
HWC5JBCX2_l01_n02_SM2_455_P1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWC5JBCX2_l01_n02_SM2_455_P1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1150287
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAAGAAATACAGTAAAATTGAATTTAATGCGATTCTTCTACCTCCAAAA60010.5216958898083696No Hit
GATATACAGAAAGCACTAATTGGTGCCTCTATCTGCTTTTTAAAACCCAA56790.49370287589097334No Hit
ATATAGTAGCGCTGAGCTTTCATGGCCTTCTGCTATTTCAAATGCTTCAT50750.4411942410893977No Hit
GAATAAAGGGGCCAAATAAAGAGACAATAAACAGAGAGGTATCAATTTTG48270.4196344042834527No Hit
CTATAGAACATGCTTGTAAATTCAAACATTCCAGTTTCGTTACAGTAACT44160.3839041908671488No Hit
CTACTATATTGTCTCATGGTCATGTACCTGAATCCTGGAAATTATTCAAT35580.3093141103046457No Hit
CATTATAGGTATTTCTTCACAAGAGCTGAATTTCCCATAGAGCTCTGCTT33390.29027538344778303No Hit
GCCTTGGAATGGATAAAAAACAAAAGATGCTTAACTGATATACAGAAAGC33300.2894929700153092No Hit
GTGTGAATGTGATGCTTGTTTTTCGCACAAAGCACAGAGCGTTCCTAGTT32590.2833205973813492No Hit
GCTTTAGCACTTCCATTACATCCTTTGCAATTCCTTCCCCATTCTTCTGA28810.25045923321744923No Hit
ACTATATTGTCTCATGGTCATGTACCTGAATCCTGGAAATTATTCAATGC24500.21299032328453682No Hit
GCTATGAGCTCTGTGTGAATGTGATGCTTGTTTTTCGCACAAAGCACAGA23950.20820890786386356No Hit
ATATTACTCTTAGTGCTTTTCCAAAGGATCCTTTTACTAGTTTCCCTTCT23380.2032536227915294No Hit
ATATTGTCTCATGGTCATGTACCTGAATCCTGGAAATTATTCAATGCAAG23370.203166687965699No Hit
CATTCACACAGAGCTCATAGCAGAGCAGCGAGATCCTCAGTGCCAGGAGT22380.1945601402084871No Hit
CTGATATACAGAAAGCACTAATTGGTGCCTCTATCTGCTTTTTAAAACCC22100.19212596508523525No Hit
AAATTACACTGTTGGTTCGGTGGGAAAGAATTTGACCTAGACTCTGCCTT22030.1915174213044223No Hit
GTTCATAGCTGAGACCATCTGCATTTCCCGTCTCACTCCTGGCACTGAGG22020.19143048647859184No Hit
CTTCTGACTTGCCCCAAGGGATCTCAATACTCCAATGTTGCTTTGCAGTT22010.19134355165276143No Hit
TTCTAATGGTATGCTGAACAGATCAGGACAAGGTATTTGAGCCTTTAGTC20630.17934654568816302No Hit
GACAATAAACAGAGAGGTATCAATTTTGAGACACAGTTACCAAAAAGAAA20200.17560834817745485No Hit
ATTACATCCTTTGCAATTCCTTCCCCATTCTTCTGACTTGCCCCAAGGGA20010.1739565864866768No Hit
GCACAGAGCGTTCCTAGTTTTACTTGCATTGAATAATTTCCAGGATTCAG19560.17004451932430775No Hit
GACCTAGACTCTGCCTTGGAATGGATAAAAAACAAAAGATGCTTAACTGA19490.1694359755434948No Hit
ATTACTATGTGGTCACTCAATACTTCCATGTTGTCAGAGAGTACTTCCTT19260.16743647454939506No Hit
GTTCTATAGAGATGGATTTGTATCTAACTTTGCAATGGAAATTCCTTCAT19130.16630632181359956No Hit
GCACTAATTGGTGCCTCTATCTGCTTTTTAAAACCCAAAGACCAGGAAAG18790.16335053773536518No Hit
ATACTAAATGGAATGAATGCTTAAATCCACGAATCTTTTTGGCTATGACT18610.16178571087041754No Hit
GTCATGTACCTGAATCCTGGAAATTATTCAATGCAAGTAAAACTAGGAAC18190.1581344481855398No Hit
ATATGGAACTTCTGGTAGCTCATGGTTGTAAATGTGAGGCCCTATTGGTC18170.15796057853387893No Hit
GCTATGAACACAGCAAAAACAATGAATGGAATGGGAAAAGGAGAAGACGT18040.15683042579808343No Hit
CAATAAACAGAGAGGTATCAATTTTGAGACACAGTTACCAAAAAGAAATT17990.15639575166893133No Hit
GCTCATAGCAGAGCAGCGAGATCCTCAGTGCCAGGAGTGAGACGGGAAAT17510.152222880029071No Hit
GTCCTATTGTCCAAGCCATGAAATGGAGAGCTGATAAAATAAAAGAACAA17480.15196207555157976No Hit
ACAATAAACAGAGAGGTATCAATTTTGAGACACAGTTACCAAAAAGAAAT17440.15161433624825804No Hit
GAACTAATGATATTCAGCTACAATCAAGACTATTCGTTAAGTAATGAATC17200.14952790042832786No Hit
TTATTATTGTCATTCCTATTGCCATATCTGCTGATTCATTTACTCCAGCA16720.14535502878846757No Hit
GAATAATTTCCAGGATTCAGGTACATGACCATGAGACAATATAGTAGCGC16340.1420515054069115No Hit
GTATTTACACAGTCTCTCCAAAGTTTCCATAGGAAATTTTCGTTAGCTTT16250.14126909197443768No Hit
GTCTCATGGTCATGTACCTGAATCCTGGAAATTATTCAATGCAAGTAAAA15920.13840024272203372No Hit
CTCCAATGTTGCTTTGCAGTTCTTCTGCCAGTTTTTGGACGTCTTCTCCT15650.1360530024246123No Hit
GTATAGCACCTGTCTTGTTCTCCAACAAAATAGCCAGATTAGGGAAAGGA15580.1354444586437993No Hit
GTTTAATATGCTATCTACCGTGTTGGGAGTAGCAGCACTAGGCATCAAAA15530.1350097845146472No Hit
CAATTACTATGTGGTCACTCAATACTTCCATGTTGTCAGAGAGTACTTCC15310.13309721834637792No Hit
CCATTAGAAAGATATAATGAAGAAACAAGGGCGAAATTAAAAAGGCTGAA15200.13214093526224324No Hit
GGTCTATATTGTCTATGTAGCTCCTCATTCCTTCAAAATTGGAGAAACCA14670.12753338949323081No Hit
TTCATGGCCTTCTGCTATTTCAAATGCTTCATGAAAGCTCACACATTTTC14500.12605549745411362No Hit
ATGCTTAACTGATATACAGAAAGCACTAATTGGTGCCTCTATCTGCTTTT13900.12083940790428822No Hit
GTATAGATCTGTTCCTTTTGGTTCTCCAACTATGAGTTCCAGACACTGCA13840.1203177989493057No Hit
GATATATCCCTTAGTCTAGATATTGTTTGTCCAAGTCTAAAGTCAATTCC13790.11988312482015358No Hit
CTCCAAAACTGTTTCACCCATTTTTATTATCTCTTCAGCAGAAAGCCCCT13780.11979618999432315No Hit
ATACTATACTGCTCTATTACTCAACTGCTGCTTCTAGTTTGGCTGTAACA13590.11814442830354513No Hit
GTACATGACCATGAGACAATATAGTAGCGCTGAGCTTTCATGGCCTTCTG13500.1173620148710713No Hit
GTTCAAACATTATAGGTATTTCTTCACAAGAGCTGAATTTCCCATAGAGC13380.11631879696110622No Hit
GATCAGGACAAGGTATTTGAGCCTTTAGTCTTTTTGTTTTACTTGTTATC13200.1147539700961586No Hit
AAGAAATACAGTAAAATTGAATTTAATGCGATTCTTCTACCTCCAAAACT13170.11449316561866735No Hit
GTTCTTTATTATTGTCATTCCTATTGCCATATCTGCTGATTCATTTACTC13090.11379768701202395No Hit
TTCTTAAAATGTCGCTGTTTGGAGACACAATTGCCTACCTGCTTTCATTG12980.1128414039278893No Hit
AGACAATATAGTAGCGCTGAGCTTTCATGGCCTTCTGCTATTTCAAATGC12940.11249366462456761No Hit
GTATAGTATGGTTATCCAATCCATCATCATTTAAAGATGCAGCAGTAATG12760.11092883775962No Hit
GTTTAGGATTGGCTCCCTATTTGTGAGTGGAAGGGAAAAATCTGTGTACC12660.11005948950131576No Hit
CCATTACATCCTTTGCAATTCCTTCCCCATTCTTCTGACTTGCCCCAAGG12640.1098856198496549No Hit
GGTTCAAACATTATAGGTATTTCTTCACAAGAGCTGAATTTCCCATAGAG12460.10832079298470729No Hit
CAATACTCCAATGTTGCTTTGCAGTTCTTCTGCCAGTTTTTGGACGTCTT12410.10788611885555519No Hit
ATTCATCACAGAGCCCTTATCGGGAATGGGAACAACAGCAACAAAAAAGA12390.10771224920389433No Hit
GATTATAGGTACACATACAAATGCCACAGAGGAGATTCCAAAGTGGAAGG12270.10666903129392924No Hit
CTTTTATTACAAGAAACTTCCAGACTACAATAATACAAAAAGCCAAAAAC12180.10588661786145545No Hit
CAATAATACAAAAAGCCAAAAACACAATGGCAGAATTTAGTGAAGATCCT12090.10510420442898165No Hit
GATCTATACTAAATACTGATCAGAGGAACATGATTCTTGAAGAACAATGC12040.1046695302998295No Hit
GTCTTAGATCCTCCCATTTCAACTTTTTAGGTGTGACTGATACTAAGGGA12020.10449566064816868No Hit
CTGTTAAAACAGGCCTCAAAAAGATTGCAACATTTAGCGTAGCATTGTTC11880.10327857308654274No Hit
CTATTGTCCAAGCCATGAAATGGAGAGCTGATAAAATAAAAGAACAAATT11870.10319163826071232No Hit
CAATATAGTAGCGCTGAGCTTTCATGGCCTTCTGCTATTTCAAATGCTTC11860.10310470343488191No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACGGCC100.0070719924145.13022145
GCTAGCC100.0070719924145.13022145
CTACCAA100.0070719924145.13022145
CGCACCC100.0070719924145.13022145
GCTACCC100.0070719924145.13022145
AGCACGC1650.0109.765531
GCACGCA1750.0103.493222
TGACGCC850.0102.44487145
CACGCAC1850.097.898993
GCTGCCC900.096.75349145
ACACGTG607.2759576E-1296.597877
GAGCAGA1000.094.178821
AAACACG1850.093.987125
CGATAAA406.085447E-790.70639145
ACAACGC1300.089.167268
ACACGAG1300.089.167267
AACACGT651.6370905E-1189.167266
AACACGA1300.089.167266
GGCACCC259.3536894E-487.07814145
CTAGGCC259.3536894E-487.07814145
Produced by FastQC (version 0.11.5)