FastQCFastQC Report
Thu 11 Jul 2019
HWC5JBCX2_l01_n02_SM2_440_P1_DM2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWC5JBCX2_l01_n02_SM2_440_P1_DM2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1464841
Sequences flagged as poor quality0
Sequence length151
%GC39

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGCACGCACTTTCTTAAAATGTCGCTGTTTGGAGACACAATTGCCTACCT106730.7286115011799915No Hit
GTAAGAAATACAGTAAAAATTGAATTTAATGCGATTCTTCTACCTCCAAA52560.358810273606487No Hit
CTATAGAACATGCTTGTAAATTCAAACATTCCAGTTTCGTTACAGTAACT48630.3319814232397919No Hit
CTACTATATTGTCTCATGGTCATGTACCTGAATCCTGGAAATTATTCAAT45570.3110917840229759No Hit
GATATACAGAAAGCACTAATTGGTGCCTCTATCTGCTTTTTAAAACCCAA44460.3035141697972681No Hit
ATATAGTAGCGCTGAGCTTTCATGGCCTTCTGCTATTTCAAATGCTTCAT43020.29368375134229585No Hit
CATTATAGGTATTTCTTCACAAGAGCTGAATTTCCCATAGAGCTCTGCTT36580.24971993547422555No Hit
ACTATATTGTCTCATGGTCATGTACCTGAATCCTGGAAATTATTCAATGC34710.23695404484172683No Hit
GTTCTATAGAGATGGATTTGTATCTAACTTTGCAATGGAAATTCCTTCAT34200.23347243830559083No Hit
ATATTGTCTCATGGTCATGTACCTGAATCCTGGAAATTATTCAATGCAAG32350.22084308126274457No Hit
CCTATAATGTTTGAACCATTCCAGATTCTTTCAATTTGTTCTTTTATTTT31450.21469906972838693No Hit
CATTCACACAGAGCTCATAGCAGAGCAGCGAGATCCTCAGTGCCAGGAGT31330.21387986819047253No Hit
GCTTTAGCACTTCCATTACATCCTTTGCAATTCCTTCCCCATTCTTCTGA31050.21196839793533906No Hit
GTTCATAGCTGAGACCATCTGCATTTCCCGTCTCACTCCTGGCACTGAGG29030.1981785053804474No Hit
GTGTGAATGTGATGCTTGTTTTTCGCACAAAGCACAGAGCGTTCCTAGTT28740.19619876833048777No Hit
GCTATGAACACAGCAAAAACAATGAATGGAATGGGAAAAGGAGAAGACGT27880.19032782397543488No Hit
CCATTAGAGAGATATAATGAAGAAACAAGGGCGAAATTAAAAAGGCTGAA27410.18711928461860367No Hit
GTAGTAACAAGAGCATTTTTCAATAACGTTTTTTGTAATGATGACAAGCA27200.18568568192725352No Hit
GCCTTGGAATGGATAAAAAACAAAAGATGCTTAACTGATATACAGAAAGC26490.18083873949459361No Hit
GTTTATTGTCTCTTTATTTGGCCCCTTTATTCTTATTTTCATGTTTACTC26420.18036087193081024No Hit
AGCAGGCACTTTCTTAAAATGTCGCTGTTTGGAGACACAATTGCCTACCT26310.17960993718772209No Hit
AGTAGAAACAACGCACTTTTTCCAGTTTATTTGCTGACATTGATTACAAT26140.17844940167567674No Hit
ATTACTATGTGGTCACTCAATACTTCCATGTTGTCAGAGAGTACTTCCTT26100.17817633449637196No Hit
CTCTAATGGTATGCTGAACAGATCAGGACAAGGTATTTGAGCCTTTAGTC25590.17469472796023594No Hit
GCTCATAGCAGAGCAGCGAGATCCTCAGTGCCAGGAGTGAGACGGGAAAT25250.1723736569361453No Hit
GCTATGAGCTCTGTGTGAATGTGATGCTTGTTTTTCGCACAAAGCACAGA24600.16793631527244254No Hit
GTATAGTATGGTTATCCAATCCATCATCATTTAAAGATGCAGCAGTAATG23820.16261150527599924No Hit
GTCCTATTGTCCAAGCCATGAAATGGAGAGCTGATAAAATAAAAGAACAA23460.16015390066225618No Hit
CTTCTGACTTGCCCCAAGGGATCTCAATACTCCAATGTTGCTTTGCAGTT22230.15175708489863404No Hit
ATTACATCCTTTGCAATTCCTTCCCCATTCTTCTGACTTGCCCCAAGGGA21940.14977734784867436No Hit
GTCTCATGGTCATGTACCTGAATCCTGGAAATTATTCAATGCAAGTAAAA21120.14417947067292627No Hit
GTCATGTACCTGAATCCTGGAAATTATTCAATGCAAGTAAAACTAGGAAC20670.14110746490574744No Hit
GTATAGATCTGTTCCTTTTGGTTCTCCAACTATGAGTTCCAGACACTGCA20340.13885466067648297No Hit
CAATTACTATGTGGTCACTCAATACTTCCATGTTGTCAGAGAGTACTTCC19900.13585092170413035No Hit
CTCCAATGTTGCTTTGCAGTTCTTCTGCCAGTTTTTGGACGTCTTCTCCT18320.12506476812159134No Hit
AGCACGCACTTGAGAGAAAACTAAAGAAAATGCTGGGTCCCTCTGCTGTA18250.12458690055780798No Hit
GTACTTCCTTCATTGCTTCCTTAGCCTGAATTTCTTTTTGGTAACTGTGT17670.1206274264578886No Hit
CCTTTATTCTTATTTTCATGTTTACTCCTCTTTTTATTTGATTTAAATGT17220.11755542069070976No Hit
GTAGTAACAAGAGCCTTTTTTCATTTTAATCATTTGTCTATCACATGTAT16770.11448341492353094No Hit
CTGATATACAGAAAGCACTAATTGGTGCCTCTATCTGCTTTTTAAAACCC16510.11270847825804985No Hit
GTTTAATATGCTATCTACCGTGTTGGGAGTAGCAGCACTAGGCATCAAAA16300.11127487556669972No Hit
GTATTTAGTATAGATCTGTTCCTTTTGGTTCTCCAACTATGAGTTCCAGA15840.10813460300469471No Hit
AAATTACACTGTTGGTTCGGTGGGAAAGAATTTGACCTAGACTCTGCCTT15830.10806633620986852No Hit
CAATACTTCCATGTTGTCAGAGAGTACTTCCTTCATTGCTTCCTTAGCCT15660.10690580069782317No Hit
CTATTGTCCAAGCCATGAAATGGAGAGCTGATAAAATAAAAGAACAAATT15430.10533566441682067No Hit
GTTCAAACATTATAGGTATTTCTTCACAAGAGCTGAATTTCCCATAGAGC15400.10513086403234208No Hit
GCACTAATTGGTGCCTCTATCTGCTTTTTAAAACCCAAAGACCAAGAAAG15300.10444819608408011No Hit
GCACAGAGCGTTCCTAGTTTTACTTGCATTGAATAATTTCCAGGATTCAG15070.10287805980307761No Hit
GCTGTAGACATAGGAAACGGATGCTTCGAAACCAAACACAAATGCAACCA15020.10253672582894663No Hit
GTCCAAGCCATGAAATGGAGAGCTGATAAAATAAAAGAACAAATTGAAAG14760.10076178916346551No Hit
TTCACACAGAGCTCATAGCAGAGCAGCGAGATCCTCAGTGCCAGGAGTGA14700.10035218839450834No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGCACGC100.0071053193144.903531
AGCACGC16700.0128.851351
GCACGCA16900.0127.755192
CACGCAC17050.0127.056183
CTAGGCC600.0108.796486145
AACGGCC352.7553688E-7103.6157145
CGCACTT22950.095.977525
ACGCACT22900.095.86744
AAACACG3650.095.285545
CAAGAGG4150.089.049278
GGCACCC750.087.03719145
CGACGGG353.4305303E-582.89256145
GAAACAC4250.081.830674
CACTTTC28400.080.626367
GCCGACC451.2300425E-680.58999145
CGCACCC451.2300425E-680.58999145
GGTGCAT451.2380642E-680.501963
GCACTTT29900.077.063626
AACACGT1350.075.142866
ACAACGC5850.073.080998
Produced by FastQC (version 0.11.5)