FastQCFastQC Report
Tue 2 Jul 2019
HWC5JBCX2_l01_n02_SM2_416_P1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWC5JBCX2_l01_n02_SM2_416_P1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1712466
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTGTAAGGCTTGCATAAATGTTATTTGTTCGAAACTATTCTCTGTCGCT87450.5106670730980936No Hit
CTCCAACACCACGTCAAGCTTTCAGGTAGACTGTTTTCTTTGGCATATTC68730.4013510341227213No Hit
CCTCATAAGTATGTCCTGGAAGAGAAGGTAATGGTGAAATTTCTCCAACT64990.379511184455633No Hit
CTTATGAGGATGTCAAAAATGCAGTTGGGGTCCTCATCGGAGGACTTGAG63360.3699927473012603No Hit
GGATTGGGTGATGCCCCATTCCTTGACCGGCTACGCCGAGATCAAAAGTC45680.2667498216022975No Hit
CCCTTGGCCTCGACATCAAAACAGCCACTCTTGTTGGGAAACAAATCGTG44960.26254535856478317No Hit
CCTCTGCACTGTCTCCGAAGAAATAAGACCCTTCATTACTCATGTCAAAG42290.24695380813400092No Hit
GCATAAATGTTATTTGTTCGAAACTATTCTCTGTCGCTTTCAATCTGTGC40050.23387325646173412No Hit
TCTCTGACATGACCCTCGAGGAAATGTCACGAGACTGGTTCATGCTTATG39180.22879286362473766No Hit
AAACAAGGGTGTTTTTTATCATTAAATAAGCTGAAACGAGAAAGTTCTTA38980.22762495722542814No Hit
ATATACAACAGGATGGGAACAGTGACCACAGAAGCTGCTTTTGGTCTAGT34870.2036244807196172No Hit
TTCCTTGACCGGCTACGCCGAGATCAAAAGTCCTTAAAAGGAAGAGGCAA34530.20163903984079098No Hit
GTAGAAACAAGGGTGTTTTTTATCATTAAATAAGCTGAAACGAGAAAGTT34100.1991280410822755No Hit
CCTTATTTCCTCAAATTTCTGTCCCAATTGTTCTCGCCATTTCTCATTTC33010.19276295120603854No Hit
ACATAATGGACTCCAACACCACGTCAAGCTTTCAGGTAGACTGTTTTCTT32870.19194541672652188No Hit
ACTTCAAGCAGTAGTTGTAAGGCTTGCATAAATGTTATTTGTTCGAAACT32550.1900767664876266No Hit
CTACTAAGGGCTTTCACTGAGGAGGGAGCAATAGTTGGAGAAATTTCACC32340.1888504647683516No Hit
GTGTTACCATTCCACTCAAGTCCTCCGATGAGGACCCCAACTGCATTTTT31710.18517155961052656No Hit
ACTAAGGGCTTTCACTGAGGAGGGAGCAATAGTTGGAGAAATTTCACCAT30970.1808503059330813No Hit
GGATAGACCCATTCAAATTACTCCAAAACAGTCAAGTGGTCAGCCTGATG30300.17693781949539436No Hit
GTCCCAATAGTTCTCATTGCATGTACCATCTGCCTAGTCTGATTAGCAAC30140.17600349437594676No Hit
GTTCTACTTCAAGCAGTAGTTGTAAGGCTTGCATAAATGTTATTTGTTCG28170.16449961634274782No Hit
GGTCTATCCCGACCAGTGAGTACCCTTCCCTTTCAAAGTCATGGCCACTT27650.16146305970454303No Hit
CTTTCAATCTGTGCCGCATTTCTTCAATTAACCACCTTATTTCCTCAAAT27590.16111268778475019No Hit
GTATCAAGGTCTCTAATCGGTTGAAGATTACACTGAAGTTTGCTTCCAGT27020.15778415454671801No Hit
GGTTCATGCTTATGCCTAGGCAAAAGATAATAGGCCCTCTTTGCGTGCGA26920.15720020134706325No Hit
AAACTATTCTCTGTCGCTTTCAATCTGTGCCGCATTTCTTCAATTAACCA26730.15609069026771918No Hit
GGCATAAGCATGAACCAGTCTCGTGACATTTCCTCGAGGGTCATGTCAGA26330.1537548774691001No Hit
CCTTGATACTACTAAGGGCTTTCACTGAGGAGGGAGCAATAGTTGGAGAA25870.15106869275068818No Hit
GTACTGGAAGCAAACTTCAGTGTAATCTTCAACCGATTAGAGACCTTGAT25570.1493168331517239No Hit
GTATTGGAGTCCATGGTTTCCACATTCTCATTTGAAGCAATCTGAACTCC25130.14674743907324292No Hit
GTACCTACTTCGCGCTACATCTCTGACATGACCCTCGAGGAAATGTCACG24910.14546274203400242No Hit
CTATTGGGACTCATCCTAGTTCCAGTGCTGGTCTGAGAGATGACCTTCTT24560.14341890583521075No Hit
GTAGTTGTAAGGCTTGCATAAATGTTATTTGTTCGAAACTATTCTCTGTC23430.1368202346791119No Hit
GCTCTATGTTGACAAAATGACCATCGTCAACATCCACAGCACTCTGCTGC23400.13664504871921543No Hit
GTTGTATATGAGGCCCATGCAACTGGCAAGTGCACCAGTTGAATAACTTA23300.1360610955195607No Hit
ATTTAAAGATGAGTCTTCTAACCGAGGTCGAAACGTACGTTCTTTCTATC22660.13232379504177016No Hit
CTCTTGTTGGGAAACAAATCGTGGAATGGATTTTAAAAGAGGAATCCAGC22480.13127267928239159No Hit
CAATGGATTGGGTGATGCCCCATTCCTTGACCGGCTACGCCGAGATCAAA22390.1307471214027023No Hit
CCCTTAGTAGTATCAAGGTCTCTAATCGGTTGAAGATTACACTGAAGTTT22320.13033835416294395No Hit
GTTCTTATTTCTTGTTCTACTTCAAGCAGTAGTTGTAAGGCTTGCATAAA21710.1267762396450499No Hit
GGTCTAGTGTGTGCCACTTGTGAACAGATTGCTGATTCACAGCATCGGTC21680.12660105368515345No Hit
TCCTAGTTCCAGTGCTGGTCTGAGAGATGACCTTCTTGAAAATTTGCAGG21420.12508277536605106No Hit
GCTCTGGAGGTAGTGAAGGTCTCCCATTCTCATCACAGCTTCTCCAAGCG21330.12455721748636177No Hit
GCATGAACCAGTCTCGTGACATTTCCTCGAGGGTCATGTCAGAGATGTAG21320.12449882216639631No Hit
GTCATACTCCTCTGCACTGTCTCCGAAGAAATAAGACCCTTCATTACTCA21030.12280535788739748No Hit
CTTTTCAAACCGTATTTAAAGCGACGATAAATACATTTGAAAAAAAGACG20830.12163745148808794No Hit
GTACTATGTTCTTTTCCATGACCGCCTGATCCAATCGCACGCAAAGAGGG20640.12052794040874387No Hit
TGGTAACACGGTTCGAGTCTCTGAAAATATACAGAGATTCGCTTGGAGAA20630.12046954508877841No Hit
GTTTTTTATCATTAAATAAGCTGAAACGAGAAAGTTCTTATTTCTTGTTC20600.12029435912888198No Hit
AGATAATAGGCCCTCTTTGCGTGCGATTGGATCAGGCGGTCATGGAAAAG20540.11994398720908911No Hit
GTATATTTTCAGAGACTCGAACCGTGTTACCATTCCACTCAAGTCCTCCG20030.1169658258908498No Hit
TCTCATTTCTGCTCTGGAGGTAGTGAAGGTCTCCCATTCTCATCACAGCT20020.11690743057088432No Hit
GGATAGAGCAGTTAAACTATACAAGAAGCTTAAAAGAGAAATAACATTCC19970.11661545397105694No Hit
GTCTTCTAACCGAGGTCGAAACGTACGTTCTTTCTATCATCCCGTCAGGC19750.11533075693181645No Hit
GTTTTCTTTGGCATATTCGCAAGCGATTTGCAGACAATGGATTGGGTGAT19720.11515557097192003No Hit
CTCATGGAATGGCTAAAGACAAGACCAATCTTGTCACCTCTGACTAAGGG19550.11416285053250694No Hit
TCATTACTCATGTCAAAGGAAGGCACGATCGGGTTCGTTGCCTTTTCGTC19500.11387087393267953No Hit
GTAATCCACAATATCAGGTGCAAGATCCCAATGATGTTTGCTGCAATGAC19240.11235259561357715No Hit
GCTTATGCCTAGGCAAAAGATAATAGGCCCTCTTTGCGTGCGATTGGATC18680.10908245769551046No Hit
CATCTGTACCTACTTCGCGCTACATCTCTGACATGACCCTCGAGGAAATG18620.1087320857757176No Hit
TCACTGAGGAGGGAGCAATAGTTGGAGAAATTTCACCATTACCTTCTCTT18620.1087320857757176No Hit
CTATTGCTCCCTCCTCAGTGAAAGCCCTTAGTAGTATCAAGGTCTCTAAT18590.10855689981582116No Hit
TGATACTACTAAGGGCTTTCACTGAGGAGGGAGCAATAGTTGGAGAAATT18290.10680504021685687No Hit
ATTCTCTGTCGCTTTCAATCTGTGCCGCATTTCTTCAATTAACCACCTTA18260.10662985425696044No Hit
TGCTTATGCCTAGGCAAAAGATAATAGGCCCTCTTTGCGTGCGATTGGAT18250.10657145893699495No Hit
GTAGTATCAAGGTCTCTAATCGGTTGAAGATTACACTGAAGTTTGCTTCC18230.10645466829706401No Hit
GTTGAAGATTACACTGAAGTTTGCTTCCAGTACTATGTTCTTTTCCATGA18230.10645466829706401No Hit
GAATAAGTGCTGACCGTGCCAGGAAAATGAGGTCTTCAATCTCAGCGTTT18090.10563713381754733No Hit
ATCTTCAACCGATTAGAGACCTTGATACTACTAAGGGCTTTCACTGAGGA17990.10505318061789257No Hit
ACAATAGAGAGGATGGTGCTTTCTGCTTTTGATGAGAGAAGAAATAAATA17760.1037100882586866No Hit
GAAGAGGCAACACCCTTGGCCTCGACATCAAAACAGCCACTCTTGTTGGG17740.10359329761875564No Hit
GTCTTACTCATATCATGATTTGGCATTCCAACCTGAATGATGCCACATAT17740.10359329761875564No Hit
CACTTAGAATGACAATTGCATCTGTACCTACTTCGCGCTACATCTCTGAC17720.10347650697882468No Hit
CTCTAGCTCTATGTTGACAAAATGACCATCGTCAACATCCACAGCACTCT17700.10335971633889374No Hit
GTGCCGCATTTCTTCAATTAACCACCTTATTTCCTCAAATTTCTGTCCCA17650.10306773973906636No Hit
AAAGCAGGGTGACAAAAACATAATGGACTCCAACACCACGTCAAGCTTTC17460.10195822865972229No Hit
CTCCCATTCTCATCACAGCTTCTCCAAGCGAATCTCTGTATATTTTCAGA17440.10184143801979133No Hit
ACATAGTACTGGAAGCAAACTTCAGTGTAATCTTCAACCGATTAGAGACC17370.10143267078003301No Hit
TGTCAGAGATGTAGCGCGAAGTAGGTACAGATGCAATTGTCATTCTAAGT17250.10073192694044729No Hit
CCTCAGTGAAAGCCCTTAGTAGTATCAAGGTCTCTAATCGGTTGAAGATT17240.10067353162048182No Hit
TCTCTGTATATTTTCAGAGACTCGAACCGTGTTACCATTCCACTCAAGTC17220.10055674098055085No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACCGG100.0070763766145.1022145
ACCGGGT100.0071079773144.886128
GACCGGG100.0071092183144.877667
CGATTAA351.893568E-9124.37332145
GGCGTTA203.8799882E-4108.655071
CGTTAAT203.8799882E-4108.655073
TCGCGCC850.0102.42509145
GCGAAAG5950.093.741632
ACCGCCC406.0937236E-790.68888145
CGAAAGC6400.087.150423
TACACAA502.2586391E-887.061325145
AGACGAA608.258212E-1084.64295145
CGAGTCC950.084.006546145
GTAGAAA12550.073.302491
TCACGCC702.7994247E-972.5511145
GCAGTTT607.9959136E-872.5511145
CGCGAAA300.001930573872.5511145
TGCGGCC300.001930573872.5511145
GTGCTCC502.2973054E-672.5511145
TAGATCC607.9959136E-872.5511145
Produced by FastQC (version 0.11.5)