FastQCFastQC Report
Thu 11 Jul 2019
HWC5JBCX2_l01_n02_SM2_416_P1_DM2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWC5JBCX2_l01_n02_SM2_416_P1_DM2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1718468
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTGTAAGGCTTGCATAAATGTTATTTGTTCGAAACTATTCTCTGTCGCT87600.5097563643896773No Hit
CTCCAACACCACGTCAAGCTTTCAGGTAGACTGTTTTCTTTGGCATATTC68890.4008803189817908No Hit
CCTCATAAGTATGTCCTGGAAGAGAAGGTAATGGTGAAATTTCTCCAACT65160.3791749395391709No Hit
CTTATGAGGATGTCAAAAATGCAGTTGGGGTCCTCATCGGAGGACTTGAG63490.3694569814509202No Hit
GGATTGGGTGATGCCCCATTCCTTGACCGGCTACGCCGAGATCAAAAGTC45750.2662254985254308No Hit
CCCTTGGCCTCGACATCAAAACAGCCACTCTTGTTGGGAAACAAATCGTG45090.2623848683827688No Hit
CCTCTGCACTGTCTCCGAAGAAATAAGACCCTTCATTACTCATGTCAAAG42420.2468477737147273No Hit
GCATAAATGTTATTTGTTCGAAACTATTCTCTGTCGCTTTCAATCTGTGC40120.23346375958120838No Hit
TCTCTGACATGACCCTCGAGGAAATGTCACGAGACTGGTTCATGCTTATG39340.22892483304897152No Hit
AAACAAGGGTGTTTTTTATCATTAAATAAGCTGAAACGAGAAAGTTCTTA39090.2274700489040238No Hit
ATATACAACAGGATGGGAACAGTGACCACAGAAGCTGCTTTTGGTCTAGT34930.20326244073209393No Hit
TTCCTTGACCGGCTACGCCGAGATCAAAAGTCCTTAAAAGGAAGAGGCAA34620.2014585083923588No Hit
GTAGAAACAAGGGTGTTTTTTATCATTAAATAAGCTGAAACGAGAAAGTT34170.1988398969314529No Hit
CCTTATTTCCTCAAATTTCTGTCCCAATTGTTCTCGCCATTTCTCATTTC33090.1925552294252788No Hit
ACATAATGGACTCCAACACCACGTCAAGCTTTCAGGTAGACTGTTTTCTT32920.19156597620671434No Hit
ACTTCAAGCAGTAGTTGTAAGGCTTGCATAAATGTTATTTGTTCGAAACT32590.1896456611353834No Hit
CTACTAAGGGCTTTCACTGAGGAGGGAGCAATAGTTGGAGAAATTTCACC32420.18865640791681895No Hit
GTGTTACCATTCCACTCAAGTCCTCCGATGAGGACCCCAACTGCATTTTT31810.18510673460314653No Hit
ACTAAGGGCTTTCACTGAGGAGGGAGCAATAGTTGGAGAAATTTCACCAT31030.18056780807090966No Hit
GGATAGACCCATTCAAATTACTCCAAAACAGTCAAGTGGTCAGCCTGATG30390.17684356065984355No Hit
GTCCCAATAGTTCTCATTGCATGTACCATCTGCCTAGTCTGATTAGCAAC30180.17562154197808746No Hit
GTTCTACTTCAAGCAGTAGTTGTAAGGCTTGCATAAATGTTATTTGTTCG28220.16421603428169743No Hit
GGTCTATCCCGACCAGTGAGTACCCTTCCCTTTCAAAGTCATGGCCACTT27690.16113189189440827No Hit
CTTTCAATCTGTGCCGCATTTCTTCAATTAACCACCTTATTTCCTCAAAT27680.16107370052861036No Hit
GTATCAAGGTCTCTAATCGGTTGAAGATTACACTGAAGTTTGCTTCCAGT27070.15752402721493794No Hit
GGTTCATGCTTATGCCTAGGCAAAAGATAATAGGCCCTCTTTGCGTGCGA27000.1571166876543526No Hit
AAACTATTCTCTGTCGCTTTCAATCTGTGCCGCATTTCTTCAATTAACCA26790.15589466897259652No Hit
GGCATAAGCATGAACCAGTCTCGTGACATTTCCTCGAGGGTCATGTCAGA26370.15345063160908437No Hit
CCTTGATACTACTAAGGGCTTTCACTGAGGAGGGAGCAATAGTTGGAGAA25930.15089021151397639No Hit
GTACTGGAAGCAAACTTCAGTGTAATCTTCAACCGATTAGAGACCTTGAT25600.14896989644264544No Hit
GTATTGGAGTCCATGGTTTCCACATTCTCATTTGAAGCAATCTGAACTCC25210.146700433176527No Hit
GTACCTACTTCGCGCTACATCTCTGACATGACCCTCGAGGAAATGTCACG24970.1453038403973772No Hit
CTATTGGGACTCATCCTAGTTCCAGTGCTGGTCTGAGAGATGACCTTCTT24600.14315075986285458No Hit
GCTCTATGTTGACAAAATGACCATCGTCAACATCCACAGCACTCTGCTGC23470.13657513552769093No Hit
GTAGTTGTAAGGCTTGCATAAATGTTATTTGTTCGAAACTATTCTCTGTC23460.13651694416189303No Hit
GTTGTATATGAGGCCCATGCAACTGGCAAGTGCACCAGTTGAATAACTTA23360.13593503050391395No Hit
ATTTAAAGATGAGTCTTCTAACCGAGGTCGAAACGTACGTTCTTTCTATC22710.1321525917270499No Hit
CTCTTGTTGGGAAACAAATCGTGGAATGGATTTTAAAAGAGGAATCCAGC22490.13087238167949594No Hit
CAATGGATTGGGTGATGCCCCATTCCTTGACCGGCTACGCCGAGATCAAA22420.13046504211891055No Hit
CCCTTAGTAGTATCAAGGTCTCTAATCGGTTGAAGATTACACTGAAGTTT22390.13029046802151684No Hit
GTTCTTATTTCTTGTTCTACTTCAAGCAGTAGTTGTAAGGCTTGCATAAA21730.1264498378788549No Hit
GGTCTAGTGTGTGCCACTTGTGAACAGATTGCTGATTCACAGCATCGGTC21730.1264498378788549No Hit
TCCTAGTTCCAGTGCTGGTCTGAGAGATGACCTTCTTGAAAATTTGCAGG21510.1251696278313009No Hit
GCTCTGGAGGTAGTGAAGGTCTCCCATTCTCATCACAGCTTCTCCAAGCG21400.1245295228075239No Hit
GCATGAACCAGTCTCGTGACATTTCCTCGAGGGTCATGTCAGAGATGTAG21390.124471331441726No Hit
GTCATACTCCTCTGCACTGTCTCCGAAGAAATAAGACCCTTCATTACTCA21130.12295835593098038No Hit
CTTTTCAAACCGTATTTAAAGCGACGATAAATACATTTGAAAAAAAGACG20910.1216781458834264No Hit
GTTTTTTATCATTAAATAAGCTGAAACGAGAAAGTTCTTATTTCTTGTTC20690.12039793583587242No Hit
GTACTATGTTCTTTTCCATGACCGCCTGATCCAATCGCACGCAAAGAGGG20670.1202815531042766No Hit
TGGTAACACGGTTCGAGTCTCTGAAAATATACAGAGATTCGCTTGGAGAA20660.1202233617384787No Hit
AGATAATAGGCCCTCTTTGCGTGCGATTGGATCAGGCGGTCATGGAAAAG20590.11981602217789333No Hit
GTATATTTTCAGAGACTCGAACCGTGTTACCATTCCACTCAAGTCCTCCG20080.1168482625222No Hit
TCTCATTTCTGCTCTGGAGGTAGTGAAGGTCTCCCATTCTCATCACAGCT20050.11667368842480628No Hit
GGATAGAGCAGTTAAACTATACAAGAAGCTTAAAAGAGAAATAACATTCC20020.11649911432741256No Hit
GTCTTCTAACCGAGGTCGAAACGTACGTTCTTTCTATCATCCCGTCAGGC19840.1154516697430502No Hit
GTTTTCTTTGGCATATTCGCAAGCGATTTGCAGACAATGGATTGGGTGAT19810.11527709564565648No Hit
CTCATGGAATGGCTAAAGACAAGACCAATCTTGTCACCTCTGACTAAGGG19610.11411326832969833No Hit
TCATTACTCATGTCAAAGGAAGGCACGATCGGGTTCGTTGCCTTTTCGTC19570.11388050286650668No Hit
GTAATCCACAATATCAGGTGCAAGATCCCAATGATGTTTGCTGCAATGAC19260.11207657052677153No Hit
GCTTATGCCTAGGCAAAAGATAATAGGCCCTCTTTGCGTGCGATTGGATC18740.10905061950528029No Hit
CATCTGTACCTACTTCGCGCTACATCTCTGACATGACCCTCGAGGAAATG18670.10864327994469491No Hit
TCACTGAGGAGGGAGCAATAGTTGGAGAAATTTCACCATTACCTTCTCTT18660.10858508857889701No Hit
CTATTGCTCCCTCCTCAGTGAAAGCCCTTAGTAGTATCAAGGTCTCTAAT18600.10823594038410958No Hit
TGATACTACTAAGGGCTTTCACTGAGGAGGGAGCAATAGTTGGAGAAATT18330.10666477350756605No Hit
TGCTTATGCCTAGGCAAAAGATAATAGGCCCTCTTTGCGTGCGATTGGAT18270.10631562531277859No Hit
ATTCTCTGTCGCTTTCAATCTGTGCCGCATTTCTTCAATTAACCACCTTA18260.10625743394698069No Hit
GTTGAAGATTACACTGAAGTTTGCTTCCAGTACTATGTTCTTTTCCATGA18260.10625743394698069No Hit
GTAGTATCAAGGTCTCTAATCGGTTGAAGATTACACTGAAGTTTGCTTCC18250.10619924258118277No Hit
GAATAAGTGCTGACCGTGCCAGGAAAATGAGGTCTTCAATCTCAGCGTTT18130.10550094619160787No Hit
ATCTTCAACCGATTAGAGACCTTGATACTACTAAGGGCTTTCACTGAGGA18000.10474445843623506No Hit
GAAGAGGCAACACCCTTGGCCTCGACATCAAAACAGCCACTCTTGTTGGG17820.10369701385187272No Hit
ACAATAGAGAGGATGGTGCTTTCTGCTTTTGATGAGAGAAGAAATAAATA17800.1035806311202769No Hit
GTCTTACTCATATCATGATTTGGCATTCCAACCTGAATGATGCCACATAT17760.10334786565708526No Hit
CACTTAGAATGACAATTGCATCTGTACCTACTTCGCGCTACATCTCTGAC17750.10328967429128735No Hit
CTCTAGCTCTATGTTGACAAAATGACCATCGTCAACATCCACAGCACTCT17740.10323148292548945No Hit
GTGCCGCATTTCTTCAATTAACCACCTTATTTCCTCAAATTTCTGTCCCA17710.10305690882809572No Hit
AAAGCAGGGTGACAAAAACATAATGGACTCCAACACCACGTCAAGCTTTC17510.10189308151213755No Hit
CTCCCATTCTCATCACAGCTTCTCCAAGCGAATCTCTGTATATTTTCAGA17480.10171850741474382No Hit
ACATAGTACTGGAAGCAAACTTCAGTGTAATCTTCAACCGATTAGAGACC17470.10166031604894592No Hit
TGTCAGAGATGTAGCGCGAAGTAGGTACAGATGCAATTGTCATTCTAAGT17290.10061287146458359No Hit
TCTCTGTATATTTTCAGAGACTCGAACCGTGTTACCATTCCACTCAAGTC17270.10049648873298775No Hit
CCTCAGTGAAAGCCCTTAGTAGTATCAAGGTCTCTAATCGGTTGAAGATT17260.10043829736718984No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCGGCC100.007079081145.08368145
CTCGGCC100.007079081145.08368145
CGATTAA403.274181E-11126.94822145
CGCGAAA256.4476862E-6116.06693145
ACCGCCC203.8576708E-4108.81276145
CCTTACC203.8764844E-4108.679769
TCGCGCC950.0106.903755145
TCACGCC1150.0100.92777145
TCTGACC750.096.72246145
GAGCGAA900.096.590191
GGTGTCC259.366413E-487.0502145
CGTATCC259.366413E-487.0502145
TCTCGGG259.366413E-487.0502145
TGTAGCC259.366413E-487.0502145
AGACGAA608.258212E-1084.63214145
TGACGCC2400.081.609566145
GTGCTCC451.2290402E-680.60204145
CTAACAC554.420508E-879.0283363
GCGAAAG5150.077.365922
ATTGGGG851.8189894E-1276.80901145
Produced by FastQC (version 0.11.5)