FastQCFastQC Report
Tue 2 Jul 2019
HWC5JBCX2_l01_n02_SM2_412_P1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWC5JBCX2_l01_n02_SM2_412_P1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4898
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCCAACACCATGTCAAGCTTTCAGGTAGACTGTTTTCTTTGGCATATTC300.6124948958758677No Hit
GAGGAGAGAGCAATAGTTGGAGAAATTTCACCATTACCTTCTCTTCCAGG290.5920783993466721No Hit
GTTGTAAGGCTTGCATAAATGTTATTTGTTCGAAACTATTCTCTGTCGCT280.5716619028174765No Hit
GGTGCACTTGCCAGTTGCATGGGCCTCATATACAACAGGATGGGAACAGT240.4899959167006942No Hit
CTGCTGCTCCTGTTGATATTCTTCCCTCATGGACTCAGGCACTCCTTCCG230.46957942017149856No Hit
CCGTGCCCAGTGAGCGAGGACTGCAGCGTAGACGCTTTATCCAAAATGCC210.4287464271131074No Hit
CTCATGGACTCAGGCACTCCTTCCGTAGAAGGCCCTCTTTTCAAACCGTA180.36749693752552065No Hit
GAGCTAGGATGAGTCCCAATAGTTCTCATTGCATGTACCATCTGCCTAGT150.30624744793793385No Hit
GTCCCAATAGTTCTCATTGCATGTACCATCTGCCTAGTCTTATTAGCAAC140.28583095140873827No Hit
CTTTCAATCTGTGCCGTATTTCTTCAATTAACCACCTTATTTCCACAAAT130.2654144548795427No Hit
ATATACAACAGGATGGGAACAGTGACCACAGAAGCTGCTTTTGGTCTAGT130.2654144548795427No Hit
GTACGGTTATCACCATCAAAATGAGCAGGGGTCAGGATATGCAGCCGACC130.2654144548795427No Hit
GTTGTATATGAGGCCCATGCAACTGGCAAGTGCACCAGTTGAATAGCTTA130.2654144548795427No Hit
GGGCCAAGGAGGTGTCACTAAGCTATTCAACTGGTGCACTTGCCAGTTGC120.2449979583503471No Hit
AAACTATTCTCTGTCGCTTTCAATCTGTGCCGTATTTCTTCAATTAACCA120.2449979583503471No Hit
GGATTGGGTGATGCCCCATTCCTTGATCGGCTACGCCGAGATCAAAAGTC110.2245814618211515No Hit
CCACAGCACTCTGCTGCTCCTGTTGATATTCTTCCCTCATGGACTCAGGC110.2245814618211515No Hit
CCCTTGGCCTCGACATCAAAACAGCCACTCTTGTTGGGAAACAAATTGTG110.2245814618211515No Hit
ACTTCAAGCAGTAGTTGTAAGGCTTGCATAAATGTTATTTGTTCGAAACT110.2245814618211515No Hit
CTCATGGAATGGCTAAAGACAAGACCAATCTTGTCACCTCTGACTAAGGG110.2245814618211515No Hit
GATGGGAACAGTGACCACAGAAGCTGCTTTTGGTCTAGTTTGTGCCACTT100.2041649652919559No Hit
CCACAATATCAGGTGCAAGATCCCAATGATGTTTGCTGCTATGACGAGAG100.2041649652919559No Hit
GCACTCTGCTGCTCCTGTTGATATTCTTCCCTCATGGACTCAGGCACTCC100.2041649652919559No Hit
ACTATGTTCTTATCCATGACCGCCTGGTCCAATCGCACGCAAAGAGGGCC100.2041649652919559No Hit
GTCCTACATTGTGGAAACATCTAATTCAGACAATGGAACGTGTTACCCAG100.2041649652919559No Hit
GTAGTTGTAAGGCTTGCATAAATGTTATTTGTTCGAAACTATTCTCTGTC100.2041649652919559No Hit
GTTGGTTCTATTGGAAAATGAAAGAACTTTGGACTATCACGATTCAAATG100.2041649652919559No Hit
GGATAGACCCATTCAAATTACTCCAAAACAGTCAAGTGGTCAGCCTGATG100.2041649652919559No Hit
GTAGAGACCCATTAGAGCACATCCAGAAGCTGATTGCCCCCAGGGAGACT100.2041649652919559No Hit
CTCTTCAGCAGATTGAGAGCATGATTGAAGCCGAGTCTTCTGTCAAAGAG100.2041649652919559No Hit
GAGCAAAAGCAGGGTGACAAAAACATAATGGAATCCAACACCATGTCAAG90.18374846876276033No Hit
CTTGTGAACAGATTGCTGATTCACAGCATCGGTCTCACAGACAAATGGCT90.18374846876276033No Hit
ACACTGTAGAGACCCATTAGAGCACATCCAGAAGCTGATTGCCCCCAGGG90.18374846876276033No Hit
AGATAATAGGCCCTCTTTGCGTGCGATTGGACCAGGCGGTCATGGATAAG90.18374846876276033No Hit
AAACAAGGGTGTTTTTTATCATTAAATAAGCTGAAACGAGAAAGCTCTTA90.18374846876276033No Hit
GTGCTGGCTAGCACTACGGCAAAGGCTATGGAACAGGTGGCTGGATCGAG90.18374846876276033No Hit
GGCTAGCACTACGGCAAAGGCTATGGAACAGGTGGCTGGATCGAGTGAAC90.18374846876276033No Hit
GAGCAAAAGCAGGTAGATATTTAAAGATGAGTCTTCTAACCGAGGTCGAA90.18374846876276033No Hit
CTTATGAGGATGTCAAAAATGCAGTTGGGGTCCTCGTCGGAGGACTTGAG90.18374846876276033No Hit
GACACATTATGTATAGGTTATCATGCGAACAATTCAACAGACACTGTAGA90.18374846876276033No Hit
GTTGAATAGATCGCCAAAATCTGGTAGATCCTTGTTGATTCCAGCTTTAC90.18374846876276033No Hit
ATTCTCTGTCGCTTTCAATCTGTGCCGTATTTCTTCAATTAACCACCTTA90.18374846876276033No Hit
GGATGGGAACAGTGACCACAGAAGCTGCTTTTGGTCTAGTTTGTGCCACT90.18374846876276033No Hit
GTGTTACCATTCCACTCAAGTCCTCCGACGAGGACCCCAACTGCATTTTT90.18374846876276033No Hit
GGCCAAGGAGGTGTCACTAAGCTATTCAACTGGTGCACTTGCCAGTTGCA80.16333197223356472No Hit
GATGAATACACAGTTCACAGCAGTGGGTAAAGAGTTCAACCACCTGGAAA80.16333197223356472No Hit
GTCTTATACAATCCAGTCCTGTTAGTTCTGGATGCTGAACAAAACTCCCG80.16333197223356472No Hit
CTGTAGACACAGTACTAGAAAAGAATGTAACAGTAACACACTCTGTTAAT80.16333197223356472No Hit
CATGGATAGAGCAGTTAAACTATACAAGAAGCTCAAAAGAGAAATAACGT80.16333197223356472No Hit
GGTCTATCCCGACCAGTGAGTACCCTTCCCTTTCAAAGTCATGGCCACTT80.16333197223356472No Hit
CAGTTAAACTATACAAGAAGCTCAAAAGAGAAATAACGTTCCATGGGGCC80.16333197223356472No Hit
CTACTAAGGGCTTTCACTGAGGAGAGAGCAATAGTTGGAGAAATTTCACC80.16333197223356472No Hit
TGGCCCCGAATAGGCCTCTAGATTGAATAGACGGGACATTCCTCAATCCT80.16333197223356472No Hit
ATGTATAGGTTATCATGCGAACAATTCAACAGACACTGTAGACACAGTAC80.16333197223356472No Hit
GCTATGACGAGAGGATCACTTGAACCGCTGCATCTGCACTCCCATTCGCT70.14291547570436913No Hit
GTTGAAGATTACACTGAAGTTTGCTTCCAGTACTATGTTCTTATCCATGA70.14291547570436913No Hit
GTTCACAGCAGTGGGTAAAGAGTTCAACCACCTGGAAAAAAGAATAGAGA70.14291547570436913No Hit
GTTCATGCTTATGCCTAGGCAAAAGATAATAGGCCCTCTTTGCGTGCGAT70.14291547570436913No Hit
GGGCCTTGCTAAATGACAAACATTCCAATGGAACCATTAAAGACAGGAGC70.14291547570436913No Hit
AATAAGACTAGGCAGATGGTACATGCAATGAGAACTATTGGGACTCATCC70.14291547570436913No Hit
GTCCACGATTGCAATACAACTTGTCAGACACCCGAGGGTGCTATAAACAC70.14291547570436913No Hit
CACTTAGAATGACAATTGCATCTGTACCTACTTCGCGTTACATTTCTGAC70.14291547570436913No Hit
CTCCCATTCTCATCACAGCTTCTCCAAGCGAATCTCTGTATATTTTCAGA70.14291547570436913No Hit
GCACTGGAGCTAGGATGAGTCCCAATAGTTCTCATTGCATGTACCATCTG70.14291547570436913No Hit
CAGCATCGGTCTCACAGACAAATGGCTACTACCACCAATCCACTAATCAG70.14291547570436913No Hit
CCTCAAAGCCGAGATCGCACAGAGACTGGAAAGTGTCTTTGCAGGAAAGA70.14291547570436913No Hit
CTTCTTTCTTGATCCGTCCAGACTCAAAGTCGACTCTGGCATCAATCCGG70.14291547570436913No Hit
ATATCAGGTGCAAGATCCCAATGATGTTTGCTGCTATGACGAGAGGATCA70.14291547570436913No Hit
CTGCAGCGTAGACGCTTTATCCAAAATGCCCTAAATGGAAATGGGGACCC70.14291547570436913No Hit
GAAGAGGCAACACCCTTGGCCTCGACATCAAAACAGCCACTCTTGTTGGG70.14291547570436913No Hit
CCTCATAAGTATGTCCTGGAAGAGAAGGTAATGGTGAAATTTCTCCAACT70.14291547570436913No Hit
CATTAGAGCACATCCAGAAGCTGATTGCCCCCAGGGAGACTACCAGTACC70.14291547570436913No Hit
GGTTCATGCTTATGCCTAGGCAAAAGATAATAGGCCCTCTTTGCGTGCGA70.14291547570436913No Hit
GTCTGTGAGACCGATGCTGTGAATCAGCAATCTGTTCACAAGTGGCACAA60.12249897917517355No Hit
ATCCTACATTAATGATAAAGGGAAAGAAGTCCTCGTGCTGTGGGGCATTC60.12249897917517355No Hit
CAGACAAATGGCTACTACCACCAATCCACTAATCAGGCATGAAAACAGAA60.12249897917517355No Hit
GTGAATGCCCCACAGCACGAGGACTTCTTTCCCTTTATCATTAATGTAGG60.12249897917517355No Hit
GTGTGAATCACTCTCCACAGCAAGTTCATGGTCCTACATTGTGGAAACAT60.12249897917517355No Hit
ATTCAACTGGTGCACTTGCCAGTTGCATGGGCCTCATATACAACAGGATG60.12249897917517355No Hit
GTATTTAAAGCGACGATAAATGCATTTGAAAAAAAGACGATCAGTAATCC60.12249897917517355No Hit
GGTCTAGTTTGTGCCACTTGTGAACAGATTGCTGATTCACAGCATCGGTC60.12249897917517355No Hit
GTTTATAGCACCCTCGGGTGTCTGACAAGTTGTATTGCAATCGTGGACTG60.12249897917517355No Hit
GGCAAAAGATAATAGGCCCTCTTTGCGTGCGATTGGACCAGGCGGTCATG60.12249897917517355No Hit
GTAGTATCAAGGTCTCTAATCGGTTGAAGATTACACTGAAGTTTGCTTCC60.12249897917517355No Hit
AAAGCAGGAAGACGGAAAACAAACCTGTATGGGTTCATTATAAAAGGAAG60.12249897917517355No Hit
CTCTTGCTCTACTTCAAGCAGTAGTTGTAAGGCTTGCATAAATGTTATTT60.12249897917517355No Hit
GCAGAGGCCATGGAGGTTGCTAATAAGACTAGGCAGATGGTACATGCAAT60.12249897917517355No Hit
GGTCTGAGAGATGACCTTCTTGAAAATTTGCAGGCCTACCAGAAGCGAAT60.12249897917517355No Hit
GCATGTACCATCTGCCTAGTCTTATTAGCAACCTCCATGGCCTCTGCTGC60.12249897917517355No Hit
ATGTAGGATTGGTTGAGCTTTGGGTATGAATTTCCTTTTTTAACTAGCCA60.12249897917517355No Hit
GTTGATATTCTTCCCTCATGGACTCAGGCACTCCTTCCGTAGAAGGCCCT60.12249897917517355No Hit
ATCTAGAGGCCTATTCGGGGCCATTGCCGGTTTCATTGAAGGGGGGTGGA60.12249897917517355No Hit
CTCCTGGTTCTTATGGCCCAGTATCTGCTTCTTAGTTCCAGGGTATTGGA60.12249897917517355No Hit
GTAATCCACAATATCAGGTGCAAGATCCCAATGATGTTTGCTGCTATGAC60.12249897917517355No Hit
GCTCTATCCATGTTGTTCGGGTCCCCATTTCCATTTAGGGCATTTTGGAT60.12249897917517355No Hit
GGGATGGTAGATGGATGGTACGGTTATCACCATCAAAATGAGCAGGGGTC60.12249897917517355No Hit
CTATTGGGACTCATCCTAGCTCCAGTGCTGGTCTGAGAGATGACCTTCTT60.12249897917517355No Hit
GCTGTGAACTGTGTATTCATCTTTTCAATAACAGAATTTACTTTGTTAGT60.12249897917517355No Hit
GATAATACCAGATCCAGCATTTCTTTCCATTGTGAATGCATATCTCGGTA60.12249897917517355No Hit
GTATCAAGGTCTCTAATCGGTTGAAGATTACACTGAAGTTTGCTTCCAGT60.12249897917517355No Hit
CCCTTATACTGGAGATCCTCCATACAGCCATGGAACAGGAACAGGATACA60.12249897917517355No Hit
ATCCAGAATAGCATAACAATAGAGAGGATGGTGCTTTCTGCTTTTGATGA60.12249897917517355No Hit
CACAGATCTTGAGGCTCTCATGGAATGGCTAAAGACAAGACCAATCTTGT50.10208248264597795No Hit
CTGTTGTATATGAGGCCCATGCAACTGGCAAGTGCACCAGTTGAATAGCT50.10208248264597795No Hit
CATCCACAGCACTCTGCTGCTCCTGTTGATATTCTTCCCTCATGGACTCA50.10208248264597795No Hit
CCATAGCCTTTGCCGTAGTGCTAGCCAGCACCATTCTGTTTTCATGCCTG50.10208248264597795No Hit
GTCTTACTCATATCATGATTTGGCATTCCAACTTGAATGATGCCACATAT50.10208248264597795No Hit
CTGCATCTGCACTCCCATTCGCTTCTGGTAGGCCTGCAAATTTTCAAGAA50.10208248264597795No Hit
CTCTAATGCAAGGTTCAACACTTCCCAGAAGGTCTGGTGCCGCAGGTGCT50.10208248264597795No Hit
CTGCTGCCTGTTCACTCGATCCAGCCACCTGTTCCATAGCCTTTGCCGTA50.10208248264597795No Hit
GTGCCGTATTTCTTCAATTAACCACCTTATTTCCACAAATTTCTGTCCCA50.10208248264597795No Hit
CCTTGATACTACTAAGGGCTTTCACTGAGGAGAGAGCAATAGTTGGAGAA50.10208248264597795No Hit
TCTCATTTCTGCTCTGGAGGTAGTGAAGGTCTCCCATTCTCATCACAGCT50.10208248264597795No Hit
GCTCTACTTCAAGCAGTAGTTGTAAGGCTTGCATAAATGTTATTTGTTCG50.10208248264597795No Hit
ATCCCAATGATGTTTGCTGCTATGACGAGAGGATCACTTGAACCGCTGCA50.10208248264597795No Hit
GGATAGAGCAGTTAAACTATACAAGAAGCTCAAAAGAGAAATAACGTTCC50.10208248264597795No Hit
ATCTTGATGTCCCCACAAAAACATATGCATCTGCATTCTGATAGAGACTT50.10208248264597795No Hit
GCATAAATGTTATTTGTTCGAAACTATTCTCTGTCGCTTTCAATCTGTGC50.10208248264597795No Hit
TGCCCCATTCCTTGATCGGCTACGCCGAGATCAAAAGTCCTTAAAAGGAA50.10208248264597795No Hit
GCCTAGTCTTATTAGCAACCTCCATGGCCTCTGCTGCCTGTTCACTCGAT50.10208248264597795No Hit
GTTCTTATCCATGACCGCCTGGTCCAATCGCACGCAAAGAGGGCCTATTA50.10208248264597795No Hit
GCTTTACCCCATCTATTTTTTCTCTGTTTAATTTTGCTTCCTCTGAGTAT50.10208248264597795No Hit
CTCTTCTTTCTTGATCCGTCCAGACTCAAAGTCGACTCTGGCATCAATCC50.10208248264597795No Hit
ATCCACAGCACTCTGCTGCTCCTGTTGATATTCTTCCCTCATGGACTCAG50.10208248264597795No Hit
CTTCATTACTCATGTCAAAGGAAGGCACGATCGGGTTCGTTGCCTTTTCG50.10208248264597795No Hit
GTGCTAGCCAGCACCATTCTGTTTTCATGCCTGATTAGTGGATTGGTGGT50.10208248264597795No Hit
ATCCAGCCACCTGTTCCATAGCCTTTGCCGTAGTGCTAGCCAGCACCATT50.10208248264597795No Hit
GATTCGCTTGGAGAAGCTGTGATGAGAATGGGAGACCTTCACTACCTCCA50.10208248264597795No Hit
GGTGATGCCCCATTCCTTGATCGGCTACGCCGAGATCAAAAGTCCTTAAA50.10208248264597795No Hit
GTTTTTTATCATTAAATAAGCTGAAACGAGAAAGCTCTTATCTCTTGCTC50.10208248264597795No Hit
ATACAAGAAGCTCAAAAGAGAAATAACGTTCCATGGGGCCAAGGAGGTGT50.10208248264597795No Hit
ATTCTACACTGTAGAGACCCATTAGAGCACATCCAGAAGCTGATTGCCCC50.10208248264597795No Hit
GAGCAGTTAAACTATACAAGAAGCTCAAAAGAGAAATAACGTTCCATGGG50.10208248264597795No Hit
CTCCTTCCGTAGAAGGCCCTCTTTTCAAACCGTATTTAAAGCGACGATAA50.10208248264597795No Hit
TATGTATCCCATCTGATATTCCAGATTCTGGTTGAAAGACACCCACGGTC50.10208248264597795No Hit
CTGTTAATCTTCTGGAAGACAAGCATAACGGAAAACTATGCAAACTAAGA50.10208248264597795No Hit
GATCTGGCCTGCGGATGCCTTCTGTTGATTGGTATTTCCTCCACTCCTGG50.10208248264597795No Hit
CCACAGAAGCTGCTTTTGGTCTAGTTTGTGCCACTTGTGAACAGATTGCT50.10208248264597795No Hit
GTACTGGAAGCAAACTTCAGTGTAATCTTCAACCGATTAGAGACCTTGAT50.10208248264597795No Hit
TCCATGGCCTCTGCTGCCTGTTCACTCGATCCAGCCACCTGTTCCATAGC50.10208248264597795No Hit
GGTCGGGATAGACCCATTCAAATTACTCCAAAACAGTCAAGTGGTCAGCC50.10208248264597795No Hit
GGCTATGGAACAGGTGGCTGGATCGAGTGAACAGGCAGCAGAGGCCATGG50.10208248264597795No Hit
CCGTGCCCAGTGAGCGTGAACACAAATCCTAAAATTCCCTTAGTCAGAGG50.10208248264597795No Hit
CAATAGTTCTCATTGCATGTACCATCTGCCTAGTCTTATTAGCAACCTCC50.10208248264597795No Hit
CAGTAATCCACAATATCAGGTGCAAGATCCCAATGATGTTTGCTGCTATG50.10208248264597795No Hit
ATGTAACATTGCTGGCTGGATCCTGGGAAATCCAGAGTGTGAATCACTCT50.10208248264597795No Hit
GAGCAATAGTTGGAGAAATTTCACCATTACCTTCTCTTCCAGGACATACT50.10208248264597795No Hit
ACTAAGGGCTTTCACTGAGGAGAGAGCAATAGTTGGAGAAATTTCACCAT50.10208248264597795No Hit
CACCATGTCAAGCTTTCAGGTAGACTGTTTTCTTTGGCATATTCGCAAGC50.10208248264597795No Hit
ATCATGACTCGAACAAAGGTGTAACGGCAGCATGTCCTCACGCTGGAGCA50.10208248264597795No Hit
GCTATACCCTGACCACTCATTTATTCCTACGATATCTTGCTTTATTGAGA50.10208248264597795No Hit
GCTCTATGTTGACAAAATGACCATCGTCAACATCCACAGCACTCTGCTGC50.10208248264597795No Hit
CTCCATGGCCTCTGCTGCCTGTTCACTCGATCCAGCCACCTGTTCCATAG50.10208248264597795No Hit
GACTCAGGCACTCCTTCCGTAGAAGGCCCTCTTTTCAAACCGTATTTAAA50.10208248264597795No Hit
GGAAAGAACACAGATCTTGAGGCTCTCATGGAATGGCTAAAGACAAGACC50.10208248264597795No Hit
TACATATGCAGTGGGGTTTTCGGAGACAATCCACGCCCTAATGATAAGAC50.10208248264597795No Hit
ATCCATGACCGCCTGGTCCAATCGCACGCAAAGAGGGCCTATTATCTTTT50.10208248264597795No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AATCCGG100.00687628144.999987
CAATCCG100.00687628144.999986
TCAATCC100.00687628144.999985
TCCGGGC100.00687628144.999989
ATCCGGG100.00687628144.999988
TGCAGCC100.00687628144.999984
GCATCAA100.00687628144.999982
TGATGAG300.001452731324.166668110-114
Produced by FastQC (version 0.11.5)