FastQCFastQC Report
Thu 11 Jul 2019
HWC5JBCX2_l01_n02_SM2_412_P1_DM2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWC5JBCX2_l01_n02_SM2_412_P1_DM2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5074
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCCAACACCATGTCAAGCTTTCAGGTAGACTGTTTTCTTTGGCATATTC300.5912495072920773No Hit
GAGGAGAGAGCAATAGTTGGAGAAATTTCACCATTACCTTCTCTTCCAGG290.5715411903823414No Hit
GTTGTAAGGCTTGCATAAATGTTATTTGTTCGAAACTATTCTCTGTCGCT280.5518328734726055No Hit
GGTGCACTTGCCAGTTGCATGGGCCTCATATACAACAGGATGGGAACAGT240.4729996058336618No Hit
CTGCTGCTCCTGTTGATATTCTTCCCTCATGGACTCAGGCACTCCTTCCG230.4532912889239259No Hit
CCGTGCCCAGTGAGCGAGGACTGCAGCGTAGACGCTTTATCCAAAATGCC210.4138746551044541No Hit
CTCATGGACTCAGGCACTCCTTCCGTAGAAGGCCCTCTTTTCAAACCGTA180.35474970437524633No Hit
GAGCTAGGATGAGTCCCAATAGTTCTCATTGCATGTACCATCTGCCTAGT150.29562475364603863No Hit
GTCCCAATAGTTCTCATTGCATGTACCATCTGCCTAGTCTTATTAGCAAC140.27591643673630273No Hit
CTTTCAATCTGTGCCGTATTTCTTCAATTAACCACCTTATTTCCACAAAT130.2562081198265668No Hit
ATATACAACAGGATGGGAACAGTGACCACAGAAGCTGCTTTTGGTCTAGT130.2562081198265668No Hit
GTACGGTTATCACCATCAAAATGAGCAGGGGTCAGGATATGCAGCCGACC130.2562081198265668No Hit
GTTGTATATGAGGCCCATGCAACTGGCAAGTGCACCAGTTGAATAGCTTA130.2562081198265668No Hit
GGGCCAAGGAGGTGTCACTAAGCTATTCAACTGGTGCACTTGCCAGTTGC120.2364998029168309No Hit
AAACTATTCTCTGTCGCTTTCAATCTGTGCCGTATTTCTTCAATTAACCA120.2364998029168309No Hit
GGATTGGGTGATGCCCCATTCCTTGATCGGCTACGCCGAGATCAAAAGTC110.21679148600709497No Hit
CCACAGCACTCTGCTGCTCCTGTTGATATTCTTCCCTCATGGACTCAGGC110.21679148600709497No Hit
CCCTTGGCCTCGACATCAAAACAGCCACTCTTGTTGGGAAACAAATTGTG110.21679148600709497No Hit
ACTTCAAGCAGTAGTTGTAAGGCTTGCATAAATGTTATTTGTTCGAAACT110.21679148600709497No Hit
CTCATGGAATGGCTAAAGACAAGACCAATCTTGTCACCTCTGACTAAGGG110.21679148600709497No Hit
GATGGGAACAGTGACCACAGAAGCTGCTTTTGGTCTAGTTTGTGCCACTT100.1970831690973591No Hit
CCACAATATCAGGTGCAAGATCCCAATGATGTTTGCTGCTATGACGAGAG100.1970831690973591No Hit
GCACTCTGCTGCTCCTGTTGATATTCTTCCCTCATGGACTCAGGCACTCC100.1970831690973591No Hit
ACTATGTTCTTATCCATGACCGCCTGGTCCAATCGCACGCAAAGAGGGCC100.1970831690973591No Hit
GTCCTACATTGTGGAAACATCTAATTCAGACAATGGAACGTGTTACCCAG100.1970831690973591No Hit
GTAGTTGTAAGGCTTGCATAAATGTTATTTGTTCGAAACTATTCTCTGTC100.1970831690973591No Hit
GTTGGTTCTATTGGAAAATGAAAGAACTTTGGACTATCACGATTCAAATG100.1970831690973591No Hit
GGATAGACCCATTCAAATTACTCCAAAACAGTCAAGTGGTCAGCCTGATG100.1970831690973591No Hit
GTAGAGACCCATTAGAGCACATCCAGAAGCTGATTGCCCCCAGGGAGACT100.1970831690973591No Hit
CTCTTCAGCAGATTGAGAGCATGATTGAAGCCGAGTCTTCTGTCAAAGAG100.1970831690973591No Hit
GAGCAAAAGCAGGGTGACAAAAACATAATGGAATCCAACACCATGTCAAG90.17737485218762317No Hit
CTTGTGAACAGATTGCTGATTCACAGCATCGGTCTCACAGACAAATGGCT90.17737485218762317No Hit
ACACTGTAGAGACCCATTAGAGCACATCCAGAAGCTGATTGCCCCCAGGG90.17737485218762317No Hit
AGATAATAGGCCCTCTTTGCGTGCGATTGGACCAGGCGGTCATGGATAAG90.17737485218762317No Hit
AAACAAGGGTGTTTTTTATCATTAAATAAGCTGAAACGAGAAAGCTCTTA90.17737485218762317No Hit
GTGCTGGCTAGCACTACGGCAAAGGCTATGGAACAGGTGGCTGGATCGAG90.17737485218762317No Hit
GGCTAGCACTACGGCAAAGGCTATGGAACAGGTGGCTGGATCGAGTGAAC90.17737485218762317No Hit
GAGCAAAAGCAGGTAGATATTTAAAGATGAGTCTTCTAACCGAGGTCGAA90.17737485218762317No Hit
CTTATGAGGATGTCAAAAATGCAGTTGGGGTCCTCGTCGGAGGACTTGAG90.17737485218762317No Hit
GACACATTATGTATAGGTTATCATGCGAACAATTCAACAGACACTGTAGA90.17737485218762317No Hit
GTTGAATAGATCGCCAAAATCTGGTAGATCCTTGTTGATTCCAGCTTTAC90.17737485218762317No Hit
ATTCTCTGTCGCTTTCAATCTGTGCCGTATTTCTTCAATTAACCACCTTA90.17737485218762317No Hit
GGATGGGAACAGTGACCACAGAAGCTGCTTTTGGTCTAGTTTGTGCCACT90.17737485218762317No Hit
GTGTTACCATTCCACTCAAGTCCTCCGACGAGGACCCCAACTGCATTTTT90.17737485218762317No Hit
GGCCAAGGAGGTGTCACTAAGCTATTCAACTGGTGCACTTGCCAGTTGCA80.15766653527788727No Hit
GATGAATACACAGTTCACAGCAGTGGGTAAAGAGTTCAACCACCTGGAAA80.15766653527788727No Hit
GTCTTATACAATCCAGTCCTGTTAGTTCTGGATGCTGAACAAAACTCCCG80.15766653527788727No Hit
CTGTAGACACAGTACTAGAAAAGAATGTAACAGTAACACACTCTGTTAAT80.15766653527788727No Hit
CATGGATAGAGCAGTTAAACTATACAAGAAGCTCAAAAGAGAAATAACGT80.15766653527788727No Hit
GGTCTATCCCGACCAGTGAGTACCCTTCCCTTTCAAAGTCATGGCCACTT80.15766653527788727No Hit
CAGTTAAACTATACAAGAAGCTCAAAAGAGAAATAACGTTCCATGGGGCC80.15766653527788727No Hit
CTACTAAGGGCTTTCACTGAGGAGAGAGCAATAGTTGGAGAAATTTCACC80.15766653527788727No Hit
TGGCCCCGAATAGGCCTCTAGATTGAATAGACGGGACATTCCTCAATCCT80.15766653527788727No Hit
ATGTATAGGTTATCATGCGAACAATTCAACAGACACTGTAGACACAGTAC80.15766653527788727No Hit
GCTATGACGAGAGGATCACTTGAACCGCTGCATCTGCACTCCCATTCGCT70.13795821836815136No Hit
GTTGAAGATTACACTGAAGTTTGCTTCCAGTACTATGTTCTTATCCATGA70.13795821836815136No Hit
GTTCACAGCAGTGGGTAAAGAGTTCAACCACCTGGAAAAAAGAATAGAGA70.13795821836815136No Hit
GTTCATGCTTATGCCTAGGCAAAAGATAATAGGCCCTCTTTGCGTGCGAT70.13795821836815136No Hit
GGGCCTTGCTAAATGACAAACATTCCAATGGAACCATTAAAGACAGGAGC70.13795821836815136No Hit
AATAAGACTAGGCAGATGGTACATGCAATGAGAACTATTGGGACTCATCC70.13795821836815136No Hit
GTCCACGATTGCAATACAACTTGTCAGACACCCGAGGGTGCTATAAACAC70.13795821836815136No Hit
CACTTAGAATGACAATTGCATCTGTACCTACTTCGCGTTACATTTCTGAC70.13795821836815136No Hit
CTCCCATTCTCATCACAGCTTCTCCAAGCGAATCTCTGTATATTTTCAGA70.13795821836815136No Hit
GCACTGGAGCTAGGATGAGTCCCAATAGTTCTCATTGCATGTACCATCTG70.13795821836815136No Hit
CAGCATCGGTCTCACAGACAAATGGCTACTACCACCAATCCACTAATCAG70.13795821836815136No Hit
CCTCAAAGCCGAGATCGCACAGAGACTGGAAAGTGTCTTTGCAGGAAAGA70.13795821836815136No Hit
CTTCTTTCTTGATCCGTCCAGACTCAAAGTCGACTCTGGCATCAATCCGG70.13795821836815136No Hit
ATATCAGGTGCAAGATCCCAATGATGTTTGCTGCTATGACGAGAGGATCA70.13795821836815136No Hit
CTGCAGCGTAGACGCTTTATCCAAAATGCCCTAAATGGAAATGGGGACCC70.13795821836815136No Hit
GAAGAGGCAACACCCTTGGCCTCGACATCAAAACAGCCACTCTTGTTGGG70.13795821836815136No Hit
CCTCATAAGTATGTCCTGGAAGAGAAGGTAATGGTGAAATTTCTCCAACT70.13795821836815136No Hit
CATTAGAGCACATCCAGAAGCTGATTGCCCCCAGGGAGACTACCAGTACC70.13795821836815136No Hit
GGTTCATGCTTATGCCTAGGCAAAAGATAATAGGCCCTCTTTGCGTGCGA70.13795821836815136No Hit
GTCTGTGAGACCGATGCTGTGAATCAGCAATCTGTTCACAAGTGGCACAA60.11824990145841545No Hit
ATCCTACATTAATGATAAAGGGAAAGAAGTCCTCGTGCTGTGGGGCATTC60.11824990145841545No Hit
CAGACAAATGGCTACTACCACCAATCCACTAATCAGGCATGAAAACAGAA60.11824990145841545No Hit
GTGAATGCCCCACAGCACGAGGACTTCTTTCCCTTTATCATTAATGTAGG60.11824990145841545No Hit
GTGTGAATCACTCTCCACAGCAAGTTCATGGTCCTACATTGTGGAAACAT60.11824990145841545No Hit
ATTCAACTGGTGCACTTGCCAGTTGCATGGGCCTCATATACAACAGGATG60.11824990145841545No Hit
GTATTTAAAGCGACGATAAATGCATTTGAAAAAAAGACGATCAGTAATCC60.11824990145841545No Hit
GGTCTAGTTTGTGCCACTTGTGAACAGATTGCTGATTCACAGCATCGGTC60.11824990145841545No Hit
GTTTATAGCACCCTCGGGTGTCTGACAAGTTGTATTGCAATCGTGGACTG60.11824990145841545No Hit
GGCAAAAGATAATAGGCCCTCTTTGCGTGCGATTGGACCAGGCGGTCATG60.11824990145841545No Hit
GTAGTATCAAGGTCTCTAATCGGTTGAAGATTACACTGAAGTTTGCTTCC60.11824990145841545No Hit
AAAGCAGGAAGACGGAAAACAAACCTGTATGGGTTCATTATAAAAGGAAG60.11824990145841545No Hit
CTCTTGCTCTACTTCAAGCAGTAGTTGTAAGGCTTGCATAAATGTTATTT60.11824990145841545No Hit
GCAGAGGCCATGGAGGTTGCTAATAAGACTAGGCAGATGGTACATGCAAT60.11824990145841545No Hit
GGTCTGAGAGATGACCTTCTTGAAAATTTGCAGGCCTACCAGAAGCGAAT60.11824990145841545No Hit
GCATGTACCATCTGCCTAGTCTTATTAGCAACCTCCATGGCCTCTGCTGC60.11824990145841545No Hit
ATGTAGGATTGGTTGAGCTTTGGGTATGAATTTCCTTTTTTAACTAGCCA60.11824990145841545No Hit
GTTGATATTCTTCCCTCATGGACTCAGGCACTCCTTCCGTAGAAGGCCCT60.11824990145841545No Hit
ATCTAGAGGCCTATTCGGGGCCATTGCCGGTTTCATTGAAGGGGGGTGGA60.11824990145841545No Hit
CTCCTGGTTCTTATGGCCCAGTATCTGCTTCTTAGTTCCAGGGTATTGGA60.11824990145841545No Hit
GTAATCCACAATATCAGGTGCAAGATCCCAATGATGTTTGCTGCTATGAC60.11824990145841545No Hit
GCTCTATCCATGTTGTTCGGGTCCCCATTTCCATTTAGGGCATTTTGGAT60.11824990145841545No Hit
GGGATGGTAGATGGATGGTACGGTTATCACCATCAAAATGAGCAGGGGTC60.11824990145841545No Hit
CTATTGGGACTCATCCTAGCTCCAGTGCTGGTCTGAGAGATGACCTTCTT60.11824990145841545No Hit
GCTGTGAACTGTGTATTCATCTTTTCAATAACAGAATTTACTTTGTTAGT60.11824990145841545No Hit
GATAATACCAGATCCAGCATTTCTTTCCATTGTGAATGCATATCTCGGTA60.11824990145841545No Hit
GTATCAAGGTCTCTAATCGGTTGAAGATTACACTGAAGTTTGCTTCCAGT60.11824990145841545No Hit
CCCTTATACTGGAGATCCTCCATACAGCCATGGAACAGGAACAGGATACA60.11824990145841545No Hit
ATCCAGAATAGCATAACAATAGAGAGGATGGTGCTTTCTGCTTTTGATGA60.11824990145841545No Hit
GCTCTATGTTGACAAAATGACCATCGTCAACATCCACAGCACTCTGCTGC60.11824990145841545No Hit

[OK]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers