FastQCFastQC Report
Tue 2 Jul 2019
HWC5JBCX2_l01_n02_SM2_411_P1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWC5JBCX2_l01_n02_SM2_411_P1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12115019
Sequences flagged as poor quality0
Sequence length151
%GC39

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATATACAGAAAGCACTAATTGGTGCCTCTATCTGCTTTTTAAAACCCAA607460.5014106870158438No Hit
ATATAGTAGCGCTGAGCTTTCATGGCCTTCTGCTATTTCAAATGCTTCAT586780.484340965540376No Hit
CTATAGAACATGCTTGTAAATTCAAACATTCCAGTTTCGTTACAGTAACT506450.4180348375846542No Hit
GTAAGAAATACAGTAAAATTGAATTTAATGCGATTCTTCTACCTCCAAAA495450.4089551985019586No Hit
GTGTGAATGTGATGCTTGTTTTTCGCACAAAGCACAGAGCGTTCCTAGTT447470.3693514636667099No Hit
AGCACGCACTTTCTTAAAATGTCGCTGTTTGGAGACACAATTGCCTACCT423790.3498054769868706No Hit
GCCTTGGAATGGATAAAAAACAAAAGATGCTTAACTGATATACAGAAAGC389370.3213944608753812No Hit
CATTATAGGTATTTCTTCACAAGAGCTGAATTTCCCATAGAGCTCTGCTT343140.2832352140760159No Hit
GCTTTAGCACTTCCATTACATCCTTTGCAATTCCTTCCCCATTCTTCTGA333240.27506353890158985No Hit
GAATAAAGGGGCCAAATAAAGAGACAATAAACAGAGAGGTATCAATTTTG313240.2585551042057796No Hit
GCTATGAGCTCTGTGTGAATGTGATGCTTGTTTTTCGCACAAAGCACAGA257630.2126534015340793No Hit
CTACTATATTGTCTCATGGTCATGTACCTAAATCCTGGAAATTATTCAAT250710.20694148312932897No Hit
GTTCATAGCTGAGACCATCTGCATTTCCCGTCTCACTCCTGGCACTGAGG241640.19945490799477905No Hit
AAATTACACTGTTGGTTCGGTGGGAAAGAATTTGACCTAGACTCTGCCTT236910.19555066318921993No Hit
GTTCTATAGAGATGGATTTGTATCTAACTTTGCAATGGAAATTCCTTCAT231530.19110989425604696No Hit
CTGATATACAGAAAGCACTAATTGGTGCCTCTATCTGCTTTTTAAAACCC219310.18102324065690695No Hit
GCTATGAACACAGCAAAAACAATGAATGGAATGGGAAAAGGAGAAGACGT211360.17446113786532236No Hit
GACCTAGACTCTGCCTTGGAATGGATAAAAAACAAAAGATGCTTAACTGA211000.17416398604079777No Hit
GTTTAATATGCTATCTACCGTGTTGGGAGTAGCAGCACTAGGCATCAAAA205660.16975623397701645No Hit
ACCTAAATCCTGGAAATTATTCAATGCAAGTAAAACTAGGAACGCTCTGT204970.16918669298001102No Hit
AGCAGGCACTTTCTTAAAATGTCGCTGTTTGGAGACACAATTGCCTACCT203430.16791554350843363No Hit
CTTCTGACTTGCCCCAAGGGATCTCAATACTCCAATGTTGCTTTGCAGTT201720.16650407234194187No Hit
GTCCTATTGTCCAAGCCATGAAATGGAGAGCTGATAAAATAAAAGAACAA199620.16477068669888179No Hit
CATTCACACAGAGCTCATAGCAGAGCAGCGAGATCCTCAGTGCCAGGAGT198920.16419289148452842No Hit
GCACTAATTGGTGCCTCTATCTGCTTTTTAAAACCCAAAGACCAGGAAAG198700.1640112987028745No Hit
TTCTAATGGTATGCTGAACAGATCAGGACAAGGTATTTGAGCCTTTAGTC194720.16072612019840826No Hit
CCATTAGAAAGATATAATGAAGAAACAAGGGCGAAATTAAAAAGGCTGAA193440.15966958037787643No Hit
ACTATATTGTCTCATGGTCATGTACCTAAATCCTGGAAATTATTCAATGC193220.1594879875962225No Hit
GTACTTCCTTCATTGCTTCTTTAGCCTGAATTTCTTTTTGGTAACTGTGT188700.1557570813549694No Hit
GTAGTAACAAGAGCATTTTTCAATAACGTTTCTTTGTAATGATGACAAGC187720.1549481680548747No Hit
ATATTGTCTCATGGTCATGTACCTAAATCCTGGAAATTATTCAATGCAAG183430.1514071088126234No Hit
TTCTTAAAATGTCGCTGTTTGGAGACACAATTGCCTACCTGCTTTCATTG180840.149269266519516No Hit
ATACTATACTGCTCTATTACTCAACTGCTGCTTCTAGTTTGGCTGTAACA172340.14225318177379664No Hit
GCACAGAGCGTTCCTAGTTTTACTTGCATTGAATAATTTCCAGGATTTAG170900.14106457447569828No Hit
TTCATGGCCTTCTGCTATTTCAAATGCTTCATGAAAGCTCACACATTTTC170640.14084996482465278No Hit
ATTACATCCTTTGCAATTCCTTCCCCATTCTTCTGACTTGCCCCAAGGGA170040.14035471178377848No Hit
ATTACTATGTGGTCACTCAATACTTCCATGTTGTCAGAGAGTACTTCCTT165380.13650824649965468No Hit
ATACTAAATGGAATGAATGCTTAAATCCACGAATCTTTTTGGCTATGACT161550.13334688125540703No Hit
GTATAGTATGGTTATCCAATCCATCATCATTTAAAGATGCAGCAGTAATG158610.13092014135512295No Hit
CTCCAATGTTGCTTTGCAGTTCTTCTGCCAGTTTTTGGACGTCTTCTCCT156280.12899690871306105No Hit
TTATTATTGTCATTCCTATTGCCATATCTGCTGATTCATTTACTCCAGCA154550.12756892911187345No Hit
CCTATAATGTTTGAACCATTCCAGATTCTTTCAATTTGTTCTTTTATTTT147170.12147731670911947No Hit
GCTCATAGCAGAGCAGCGAGATCCTCAGTGCCAGGAGTGAGACGGGAAAT147000.1213369950142051No Hit
GTTGTTCCCATTCCCGATAAGGGCTCTGTGATGAATCTTCTTTTTCTTTC146890.12124619862337814No Hit
GTATAGATCTGTTCCTTTTGGTTCTCCAACTATGAGTTCCAGACACTGCA145770.12032172628041278No Hit
ATGCTTAACTGATATACAGAAAGCACTAATTGGTGCCTCTATCTGCTTTT144950.11964488045788454No Hit
AGACAATATAGTAGCGCTGAGCTTTCATGGCCTTCTGCTATTTCAAATGC142990.11802705385769514No Hit
GTATAGCACCTGTCTTGTTCTCCAACAAAATAGCCAGATTAGGGAAAGGA142430.11756481768621246No Hit
ATTCATCACAGAGCCCTTATCGGGAATGGGAACAACAGCAACAAAAAAGA141590.11687146342898842No Hit
ATATTACTCTTAGTGCTTTTCCAAAGGATCCTTTTACTAGTTTCCCTTCT140980.11636795617076623No Hit
GGATTTAGGTACATGACCATGAGACAATATAGTAGCGCTGAGCTTTCATG140860.11626890556259135No Hit
CAATATAGTAGCGCTGAGCTTTCATGGCCTTCTGCTATTTCAAATGCTTC140790.11621112604115603No Hit
GGTTCAAACATTATAGGTATTTCTTCACAAGAGCTGAATTTCCCATAGAG140460.11593873686867515No Hit
CAATTACTATGTGGTCACTCAATACTTCCATGTTGTCAGAGAGTACTTCC139380.11504728139510141No Hit
GTTCAAACATTATAGGTATTTCTTCACAAGAGCTGAATTTCCCATAGAGC139330.11500601030836187No Hit
AGTAGAAACAACGCACTTTTTCCAGTTTATTTGCTGACATTGATTACAAT138920.11466758739709776No Hit
GAATAATTTCCAGGATTTAGGTACATGACCATGAGACAATATAGTAGCGC138700.11448599461544384No Hit
TAGTAGCGCTGAGCTTTCATGGCCTTCTGCTATTTCAAATGCTTCATGAA137500.11349548853369525No Hit
CTATTGTCCAAGCCATGAAATGGAGAGCTGATAAAATAAAAGAACAAATT134320.11087064741706142No Hit
CAATTGCCTACCTGCTTTCATTGACAGAAGATGGAGAAGGCAAAGCAGAA134260.110821122112974No Hit
GACAATAAACAGAGAGGTATCAATTTTGAGACACAGTTACCAAAAAGAAA133040.10981410759652957No Hit
GTCCTGATCTGTTCAGCATACCATTAGAAAGATATAATGAAGAAACAAGG130660.10784960386772814No Hit
GTCCAAGCCATGAAATGGAGAGCTGATAAAATAAAAGAACAAATTGAAAG130550.1077588074769012No Hit
GTACATGACCATGAGACAATATAGTAGCGCTGAGCTTTCATGGCCTTCTG129210.1066527423522819No Hit
CAATACTCCAATGTTGCTTTGCAGTTCTTCTGCCAGTTTTTGGACGTCTT127880.10555493144501053No Hit
GATTCTGGCTGAGAGAAAAATGAGAAAATGTGTGAGCTTTCATGAAGCAT127490.10523301696844223No Hit
CCATGAGACAATATAGTAGCGCTGAGCTTTCATGGCCTTCTGCTATTTCA126280.1042342566693457No Hit
GATTTAGGTACATGACCATGAGACAATATAGTAGCGCTGAGCTTTCATGG125730.10378027471521092No Hit
CTGTTGGTTCGGTGGGAAAGAATTTGACCTAGACTCTGCCTTGGAATGGA125260.1033923264998594No Hit
GATTCAGGTACATGACCATGAGACAATATAGTAGCGCTGAGCTTTCATGG124140.10246785415689401No Hit
ATATGGAACTTCTGGTAGCTCATGGTTGTAAATGTGAGGCCCTATTGGTC124090.1024265830701545No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGCCGC100.0070773773145.09897145
CGCACCC1900.0126.00699145
AGCACGC57100.0122.543951
GCACGCA57900.0120.475972
CACGCAC58150.0119.958993
GCGCCCG203.8566245E-4108.82422145
CGACGGG3850.0105.52651145
GAAACAC22100.096.035454
AAACACG22050.095.928675
GGCACCC3500.093.27791145
GAGCAGA9950.092.455241
ACACGTG6400.091.686657
AACACGT6450.090.974036
TGACGCC6550.090.8253145
CGATAAA4300.089.421455145
GCCGACC3700.088.235855145
ACCGGCC259.363873E-487.05938145
ATAAGCC4100.086.705475145
ACGCACT84350.084.587734
CGCACTT84450.084.31955
Produced by FastQC (version 0.11.5)