FastQCFastQC Report
Thu 11 Jul 2019
HWC5JBCX2_l01_n02_SM2_395_P1_DM2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWC5JBCX2_l01_n02_SM2_395_P1_DM2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1991123
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGCACGCACTTTCTTAAAATGTCGCTGTTTGGAGACACAATTGCCTACCT121920.6123177724329436No Hit
GTAAGAAATACAGTAAAAATTGAATTTAATGCGATTCTTCTACCTCCAAA64330.32308400837115536No Hit
CTACTATATTGTCTCATGGTCATGTACCTGAATCCTGGAAATTATTCAAT62960.3162034690975897No Hit
GATATACAGAAAGCACTAATTGGTGCCTCTATCTGCTTTTTAAAACCCAA62860.3157012399535338No Hit
ATATAGTAGCGCTGAGCTTTCATGGCCTTCTGCTATTTCAAATGCTTCAT60230.3024926134648638No Hit
CTATAGAACATGCTTGTAAATTCAAACATTCCAGTTTCGTTACAGTAACT59590.2992783469429061No Hit
CATTATAGGTATTTCTTCACAAGAGCTGAATTTCCCATAGAGCTCTGCTT50990.25608664055409935No Hit
GTAGTAACAAGAGCATTTTTCAATAACGTTTTTTGTAATGATGACAAGCA49930.2507630116271069No Hit
ACTATATTGTCTCATGGTCATGTACCTGAATCCTGGAAATTATTCAATGC44910.22555110859550112No Hit
GCTTTAGCACTTCCATTACATCCTTTGCAATTCCTTCCCCATTCTTCTGA43750.2197252505244528No Hit
ATATTGTCTCATGGTCATGTACCTGAATCCTGGAAATTATTCAATGCAAG42160.21173980713396412No Hit
CATTCACACAGAGCTCATAGCAGAGCAGCGAGATCCTCAGTGCCAGGAGT40840.20511038243242632No Hit
GTGTGAATGTGATGCTTGTTTTTCGCACAAAGCACAGAGCGTTCCTAGTT40300.20239834505452453No Hit
GTTCATAGCTGAGACCATCTGCATTTCCCGTCTCACTCCTGGCACTGAGG40060.20119299510879038No Hit
GTTCTATAGAGATGGATTTGTATCTAACTTTGCAATGGAAATTCCTTCAT39550.1986316264741053No Hit
GCCTTGGAATGGATAAAAAACAAAAGATGCTTAACTGATATACAGAAAGC36830.18497099375578505No Hit
GCTATGAGCTCTGTGTGAATGTGATGCTTGTTTTTCGCACAAAGCACAGA35960.1806016002024988No Hit
GCTATGAACACAGCAAAAACAATGAATGGAATGGGAAAAGGAGAAGACGT35240.17698555036529637No Hit
CCTATAATGTTTGAACCATTCCAGATTCTTTCAATTTGTTCTTTTATTTT35170.17663398996445723No Hit
GCTCATAGCAGAGCAGCGAGATCCTCAGTGCCAGGAGTGAGACGGGAAAT34410.17281704846963244No Hit
GTATAGTATGGTTATCCAATCCATCATCATTTAAAGATGCAGCAGTAATG34230.17191303601033187No Hit
AGCAGGCACTTTCTTAAAATGTCGCTGTTTGGAGACACAATTGCCTACCT32170.16156711564278048No Hit
GTCCTATTGTCCAAGCCATGAAATGGAGAGCTGATAAAATAAAAGAACAA32040.16091421775550782No Hit
CCATTAGAGAGATATAATGAAGAAACAAGGGCGAAATTAAAAAGGCTGAA31530.1583528491208228No Hit
CTTCTGACTTGCCCCAAGGGATCTCAATACTCCAATGTTGCTTTGCAGTT31110.15624348671578803No Hit
GTCATGTACCTGAATCCTGGAAATTATTCAATGCAAGTAAAACTAGGAAC30700.15418434722515886No Hit
ATTACTATGTGGTCACTCAATACTTCCATGTTGTCAGAGAGTACTTCCTT30250.15192431607690737No Hit
ATACTATACTGCTCTATTACTCAACTGCTGCTTCTAGTTTGGCTGTAACA29580.1485593808117329No Hit
CTCTAATGGTATGCTGAACAGGTCAGGACAAGGTATTTGAGCCTTTAGTC28700.14413976434404102No Hit
GTTTATTGTCTCTTTATTTGGCCCCTTTATTCTTATTTTCATGTTTACTC28250.1418797331957895No Hit
ATTACATCCTTTGCAATTCCTTCCCCATTCTTCTGACTTGCCCCAAGGGA28200.14162861862376158No Hit
GTATAGATCTGTTCCTTTTGGTTCTCCAACTATGAGTTCCAGACACTGCA27170.1364556584399859No Hit
GTCTCATGGTCATGTACCTGAATCCTGGAAATTATTCAATGCAAGTAAAA26810.13464763352138465No Hit
CTCCAATGTTGCTTTGCAGTTCTTCTGCCAGTTTTTGGACGTCTTCTCCT25320.12716441927495187No Hit
CAATTACTATGTGGTCACTCAATACTTCCATGTTGTCAGAGAGTACTTCC25290.1270137505317351No Hit
AGCACGCACTTGAGAGAAAACTAAAGAAAATGCTGGGTCCCTCTGCTGTA25180.12646129847327364No Hit
CTGATATACAGAAAGCACTAATTGGTGCCTCTATCTGCTTTTTAAAACCC23550.1182749634251626No Hit
GCACAGAGCGTTCCTAGTTTTACTTGCATTGAATAATTTCCAGGATTCAG23320.11711983639383403No Hit
AGTAGAAACAACGCACTTTTTCCAGTTTATTTGCTGACATTGATTACAAT22930.11516114273201605No Hit
GCACTAATTGGTGCCTCTATCTGCTTTTTAAAACCCAAAGACCAGGAAAG22870.11485980524558251No Hit
GTAGTAACAAGAGCCTTTTTTCATTTTAATCATTTGTCTATCACATGTAT22870.11485980524558251No Hit
GCTGTAGACATAGGAAACGGATGCTTCGAAACCAAACACAAATGCAACCA22170.11134420123719128No Hit
CTATTGTCCAAGCCATGAAATGGAGAGCTGATAAAATAAAAGAACAAATT21840.10968684506180683No Hit
AAATTACACTGTTGGTTCGGTGGGAAAGAATTTGACCTAGACTCTGCCTT21480.10787882014320561No Hit
CAATACTCCAATGTTGCTTTGCAGTTCTTCTGCCAGTTTTTGGACGTCTT21060.10576945773817087No Hit
GAATAAAGGGGCCAAATAAAGAGACAATAAACAGAGAGGTATCAATTTTG20900.10496589110768144No Hit
GACCTAGACTCTGCCTTGGAATGGATAAAAAACAAAAGATGCTTAACTGA20860.1047649994500591No Hit
GTCCAAGCCATGAAATGGAGAGCTGATAAAATAAAAGAACAAATTGAAAG20820.10456410779243674No Hit
GGTTCAAACATTATAGGTATTTCTTCACAAGAGCTGAATTTCCCATAGAG20430.10260541413061874No Hit
GATCTATACTAAATACTGATCAGAGGAACATGATTCTTGAAGAACAATGC20290.1019022933289405No Hit
GTTCAAACATTATAGGTATTTCTTCACAAGAGCTGAATTTCCCATAGAGC20120.10104850378404549No Hit
GTATTTAGTATAGATCTGTTCCTTTTGGTTCTCCAACTATGAGTTCCAGA20110.10099828086963988No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACGCGC100.007110179144.871693
AGCACGC19200.0127.5108641
GCACGCA19350.0126.528772
CACGCAC19400.0126.2026753
ACTCGTA203.8779408E-4108.670148
GAGCACG203.8810415E-4108.6483151
GAAACAC6200.0102.812174
ACGCACT24400.0101.8258064
CGCACTT24500.0101.415285
AAACACG6400.0100.736165
CGGTGCA301.6068985E-596.581132
CTAGGCC554.129106E-1092.348785145
ATAAGCC1000.087.07171145
CGCACCC750.087.07171145
ACGTGCA2050.084.802942
GTAACAA23050.081.392134
CTTAGGG451.2273085E-680.621956145
CAACGCA5300.077.9144449
ACAACGC5350.077.186278
CACCCCC851.8189894E-1276.82798145
Produced by FastQC (version 0.11.5)