FastQCFastQC Report
Tue 2 Jul 2019
HWC5JBCX2_l01_n02_SM2_274_P1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWC5JBCX2_l01_n02_SM2_274_P1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1562282
Sequences flagged as poor quality0
Sequence length151
%GC39

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAGTAACAAGAGCATTTTTCAATAACGTTTCTTTGTAATGACAACAAGC58290.37310805603597813No Hit
CTGTAGAACATGCTTGTAAATTCAAACATTCCAGTCTCATTACAGTAACT48650.3114034470089267No Hit
GAATATTACTGCTGCATCTTTAAATGACGATGGATTGGATAATCATACTA41640.2665331867102098No Hit
CCATTAGAAAGATATAATGAAGAAACAAGGGCAAAATTGAAGAAGCTAAA33930.21718230127467383No Hit
GTTTAATATGCTATCTACCGTGTTGGGAGTAGCTGCACTAGGTATCAAGA32660.2090531670978735No Hit
GAATACAACAATAACCTCTTTTAGGTTGAATGATTTAAATGGAGCCGACA31000.19842768463055965No Hit
ATACTATACTGCTTTACTACTCAACTGCTGCCTCCAGTTTGGCTGTAACA30590.19580331847899418No Hit
ATTATTATGTGGTCATTCAATACCTCCATGTTGTCAGAGAGCACTTCCTT27080.17333618386437274No Hit
GTTCTACAGAGATGGATTTGTATCTAATTTTGCAATGGAACTCCCTTCGT27050.1731441570727948No Hit
GTAGTAACAAGAGGATTTTTCAATAACGTTTCTTTGTAATGACAACAAGC26680.1707758266433333No Hit
GTATTTACACAGTCTCTCCAAAGCTTCCATAAGAAATTTTCGTTAGCTTT26060.1668072729507221No Hit
CCTTTATTACAAGAAACTTCCAGACTACAATAATACAAAAGGCCAAAAAC24690.15803804946866187No Hit
ATATTACTCTTAGTGCTTTTCCAAAGGATCCTTTTACTAGTTTTCCTTCT24510.1568858887191941No Hit
TTATTATTGTCATTCCTATTGCCATATCTGCTGATTCATTTACTCCAGCA23060.14760459379292598No Hit
GGATGAAGAAACATGTATGGAAGGAATAAACGACTTTTACCGAACATGTA23010.14728454914029607No Hit
GTATAGCACCGGTCCTGTTCTCCAATAAGATAGCAAGATTGGGGAAAGGA21790.13947545961612565No Hit
GTTTAGTATACCATTAGAAAGATATAATGAAGAAACAAGGGCAAAATTGA21550.13793924528350196No Hit
GTATAGTAGCGCTGAGCTTTCATGGCCTTCTGCTATTTCAAATGCTTCAT21280.13621100415930032No Hit
GTGTAATACCACTGACAACAACACCCACCAAATCTCATTTTGCAAATCTC20300.12993812896775356No Hit
AGATTATACTTCAATACTATTTCTGGGATATGCAAGTTTCTCAAATTGTA20180.12917002180144174No Hit
CAAGAGCATTTTTCAATAACGTTTCTTTGTAATGACAACAAGCAAACAAG19630.12564953062251244No Hit
ATACTAAACAGATCAGGACAAGGTATTTGAGCCTTTAGTCTTTTTGTTTT19180.12276912874884303No Hit
TCTTCATCCTTTGCATTAACAAATAGAGCAAAATCATCAGAAGATTGCAG17840.11419193205836077No Hit
CAATAATACAAAAGGCCAAAAACACAATGGCAGAATTTAGTGAAGATCCT17790.11387188740573084No Hit
GTATAGTATGATTATCCAATCCATCGTCATTTAAAGATGCAGCAGTAATA17290.1106714408794315No Hit
CTTCAATACTATTTCTGGGATATGCAAGTTTCTCAAATTGTAAATGTTGG17260.11047941408785353No Hit
GATATAATGAAGAAACAAGGGCAAAATTGAAGAAGCTAAAACCATTCTTC17120.10958328906048972No Hit
TGTTTATTCCCAATAATTTACATGTTCGGTAAAAGTCGTTTATTCCTTCC16420.10510266392367064No Hit
CTACAATAAAGGAAAATACAGGGCTTAACTTTCCTTATAGACAAATGGAG16180.10356644959104695No Hit
CTTTTAGGTTGAATGATTTAAATGGAGCCGACAAAGGTGGATTAATACCT16040.10267032456368314No Hit
CTACTATACTGTCTCATGGTCATGTACCTGAATCCTGGAAATTATTCAAT16000.10241428884157917No Hit
TTCTAATGGTATACTAAACAGATCAGGACAAGGTATTTGAGCCTTTAGTC15870.10158217274474134No Hit
GAATAGAGACTCCCAAGTATCTGGCTGATTTGTTTGATTACAAAACCAAA15830.1013261370226374No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGCACCC100.0070767747145.09908145
CGCACCC151.226794E-4145.09908145
GGACCCC100.0070767747145.09908145
GTAACAA14600.0122.557224
ATAAGCC550.0118.71743145
GCCACCC203.8559764E-4108.8243145
AGTAGCC750.0106.40598145
TTGCGCC352.7512579E-7103.642204145
TCTGACC1150.0100.938484145
TAGAAAC5150.098.465852
GAAACAC5100.098.010744
AAACACG5150.097.06545
GGTACCC607.2759576E-1296.73271145
AGCACGC1800.096.5902941
GCACGCA1850.093.979752
CACGCAC1950.092.87533
TGACGCC554.1472958E-1092.33578145
AAGCGGA406.1479477E-790.5534062
CGGAGCA651.6370905E-1189.160282
GATACCC502.258821E-887.05944145
Produced by FastQC (version 0.11.5)