FastQCFastQC Report
Thu 11 Jul 2019
HWC5JBCX2_l01_n02_SM2_274_P1_DM2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWC5JBCX2_l01_n02_SM2_274_P1_DM2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1570602
Sequences flagged as poor quality0
Sequence length151
%GC39

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAGTAACAAGAGCATTTTTCAATAACGTTTCTTTGTAATGACAACAAGC58550.37278699505030555No Hit
CTGTAGAACATGCTTGTAAATTCAAACATTCCAGTCTCATTACAGTAACT48810.31077255727421715No Hit
GAATATTACTGCTGCATCTTTAAATGACGATGGATTGGATAATCATACTA41790.2660763197805682No Hit
CCATTAGAAAGATATAATGAAGAAACAAGGGCAAAATTGAAGAAGCTAAA34110.21717787192426855No Hit
GTTTAATATGCTATCTACCGTGTTGGGAGTAGCTGCACTAGGTATCAAGA32730.20839143207508967No Hit
GAATACAACAATAACCTCTTTTAGGTTGAATGATTTAAATGGAGCCGACA31080.19788590616846277No Hit
ATACTATACTGCTTTACTACTCAACTGCTGCCTCCAGTTTGGCTGTAACA30710.1955301215712192No Hit
GTTCTACAGAGATGGATTTGTATCTAATTTTGCAATGGAACTCCCTTCGT27150.17286365355449695No Hit
ATTATTATGTGGTCATTCAATACCTCCATGTTGTCAGAGAGCACTTCCTT27130.17273631384653781No Hit
GTAGTAACAAGAGGATTTTTCAATAACGTTTCTTTGTAATGACAACAAGC26770.17044419910327377No Hit
GTATTTACACAGTCTCTCCAAAGCTTCCATAAGAAATTTTCGTTAGCTTT26150.16649666815654127No Hit
CCTTTATTACAAGAAACTTCCAGACTACAATAATACAAAAGGCCAAAAAC24810.15796490772328065No Hit
ATATTACTCTTAGTGCTTTTCCAAAGGATCCTTTTACTAGTTTTCCTTCT24610.1566915106436895No Hit
TTATTATTGTCATTCCTATTGCCATATCTGCTGATTCATTTACTCCAGCA23170.1475230516706333No Hit
GGATGAAGAAACATGTATGGAAGGAATAAACGACTTTTACCGAACATGTA23080.1469500229848173No Hit
GTATAGCACCGGTCCTGTTCTCCAATAAGATAGCAAGATTGGGGAAAGGA21890.13937331036125003No Hit
GTTTAGTATACCATTAGAAAGATATAATGAAGAAACAAGGGCAAAATTGA21610.13759055444982243No Hit
GTATAGTAGCGCTGAGCTTTCATGGCCTTCTGCTATTTCAAATGCTTCAT21340.13587146839237438No Hit
GTGTAATACCACTGACAACAACACCCACCAAATCTCATTTTGCAAATCTC20370.12969549255635737No Hit
AGATTATACTTCAATACTATTTCTGGGATATGCAAGTTTCTCAAATTGTA20250.12893145430860267No Hit
CAAGAGCATTTTTCAATAACGTTTCTTTGTAATGACAACAAGCAAACAAG19690.1253659424857475No Hit
ATACTAAACAGATCAGGACAAGGTATTTGAGCCTTTAGTCTTTTTGTTTT19260.12262813876462657No Hit
TCTTCATCCTTTGCATTAACAAATAGAGCAAAATCATCAGAAGATTGCAG17910.11403270847738638No Hit
CAATAATACAAAAGGCCAAAAACACAATGGCAGAATTTAGTGAAGATCCT17840.11358701949952948No Hit
GTATAGTATGATTATCCAATCCATCGTCATTTAAAGATGCAGCAGTAATA17400.11078554592442898No Hit
CTTCAATACTATTTCTGGGATATGCAAGTTTCTCAAATTGTAAATGTTGG17270.10995783782269473No Hit
GATATAATGAAGAAACAAGGGCAAAATTGAAGAAGCTAAAACCATTCTTC17160.1092574694289196No Hit
TGTTTATTCCCAATAATTTACATGTTCGGTAAAAGTCGTTTATTCCTTCC16450.10473690979637107No Hit
CTACAATAAAGGAAAATACAGGGCTTAACTTTCCTTATAGACAAATGGAG16280.1036545222787186No Hit
CTACTATACTGTCTCATGGTCATGTACCTGAATCCTGGAAATTATTCAAT16080.10238112519912748No Hit
CTTTTAGGTTGAATGATTTAAATGGAGCCGACAAAGGTGGATTAATACCT16070.10231745534514791No Hit
GAATAGAGACTCCCAAGTATCTGGCTGATTTGTTTGATTACAAAACCAAA15920.10136240753545456No Hit
TTCTAATGGTATACTAAACAGATCAGGACAAGGTATTTGAGCCTTTAGTC15890.10117139797351589No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTGCCC151.2270035E-4145.09288145
GCCGACC351.895387E-9124.36533145
CACGCAC1800.0120.718283
AGCACGC1800.0120.714431
TGACGCC1150.0119.859344145
GCACGCA1850.0117.4518662
AACGGCC256.445476E-6116.07431145
AAACACG4900.0115.312965
GTAACAA14150.0111.589764
CGCACTT1900.0110.5666055
CGCACCC404.796675E-9108.819664145
GCCACCC203.8566338E-4108.819664145
CGGTGCA404.8512447E-9108.642992
TAGAAAC5350.0108.3045352
AACACGT1750.0107.6357046
GAAACAC5500.0102.7202764
ACGCACT2050.0102.463324
ACACGTG2050.091.887077
TCTGACC900.088.66788145
GTGCTCC259.363897E-487.05573145
Produced by FastQC (version 0.11.5)