FastQCFastQC Report
Tue 2 Jul 2019
HWC5JBCX2_l01_n02_SM2_268_P1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWC5JBCX2_l01_n02_SM2_268_P1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1322120
Sequences flagged as poor quality0
Sequence length151
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAATAATAGTGATCAGGCAGAAAAGAAAAATGGAACATCTAAAAATGTAT101600.768462771911778No Hit
TTATAGAACTGCAGGATTCACAAGTAGTATTAGAGACATTCCACACTGCA66360.5019211569297795No Hit
GTCCTAGACAAGGCCCAGGCCAAGTTCTGCAGCGAGAACGCAGCGCAGGC53310.40321604695489066No Hit
ATCTTAGTGGGGGAAAGGCTGCTGCTCGTGCAGGTCTGGGTCCTGTCCAT43030.32546213656854145No Hit
ATATCATCCAGTGTGCAGTGTTGTGTATTTTTCTAAGCATGACAACATTG39830.3012585846973043No Hit
CTCCAGTCATATCTCTTAACAAAATGACATAAATTGACAATGTTCACTAA39180.2963422382234593No Hit
GTATTAGAGACATTCCACACTGCACACTCCAGTCATATCTCTTAACAAAA38490.29112334735122375No Hit
GTACAAACTCGAGTCAGACAAGCGCCTGGTGGAGGAGAAAGTGAACCAAC37400.2828790124950836No Hit
GTATATGGTATTGGGGGCAGGGAGAACCAGAAACCCTCAAAGAGAACCAA36620.27697939672646965No Hit
GGTCTGGGTCCTGTCCATCAGGATTTTCACAGACTTGGTGTTCTTCATGA35420.2679030647747557No Hit
GGTCTAAAGAACGCACTTGAAAGTTGCAAAATTACTTGCCAATGTTTGGG35140.26578525398602243No Hit
GTATGGAAGGGCCATCGCTCAACGGATAAAAGGTACTCTGGGGATAACAG32700.24733004568420416No Hit
GGGTATGGCTGTTCGCCATTTAAAGAGGTACGTGAGCTGGGTTTAAAACG32610.2466493207878256No Hit
CTACTATACAACTGAAGAATTGAAGGGGGGGGACACCACCAAGAACTCTT28930.21881523613590295No Hit
GTGCAGTGTTGTGTATTTTTCTAAGCATGACAACATTGATGTGCCTTTTC28030.21200798717211752No Hit
CTCTAATACTACTTGTGAATCCTGCAGTTCTATAATCATAAACAAAAATT26760.2024022025232203No Hit
GTATTGGGGGCAGGGAGAACCAGAAACCCTCAAAGAGAACCAATTTGTAG26410.19975493903730374No Hit
TGACTGGAGTGTGCAGTGTGGAATGTCTCTAATACTACTTGTGAATCCTG25630.19385532326868968No Hit
GTGGAGGAGAAAGTGAACCAACTGAAGGAGGAAGTTCGGCTGCAGTACGA25510.19294769007351828No Hit
GGCTGATACCGCCCAAGAGTTCATATCGACGGCGGTGTTTGGCACCTCGA25370.19188878467915166No Hit
GCTGTACAAACTCGAGTCAGACAAGCGCCTGGTGGAGGAGAAAGTGAACC25350.19173751247995643No Hit
TTCTAGCATTTTCCGCAGCTGCTTCTCCTTCTTGGCCACTTCGTTCAGGA25230.19082987928478504No Hit
GTACGAGAAGCTGCACCAGCTGCTGGACGAGGACCTGCGGCAGACAGTGG24260.18349317762381628No Hit
GTTCTATAATCATAAACAAAAATTACTTAGTTTCGTTAAGCTAAGATTGT24180.18288808882703536No Hit
AAGCAGGACATTGAGGACCAGCTGTACAAACTCGAGTCAGACAAGCGCCT23590.17842555895077603No Hit
GGCTATGACTTGTCGCGTCGCTGACTCGATGGTCAACGCCGCCAATATGG22690.1716183099869906No Hit
GTCCCAAGGGTATGGCTGTTCGCCATTTAAAGAGGTACGTGAGCTGGGTT22610.17101322119020967No Hit
ATTGAGGACCAGCTGTACAAACTCGAGTCAGACAAGCGCCTGGTGGAGGA22310.16874413820228118No Hit
ACATTGAGGACCAGCTGTACAAACTCGAGTCAGACAAGCGCCTGGTGGAG22170.16768523280791456No Hit
GTGTGGAATGTCTCTAATACTACTTGTGAATCCTGCAGTTCTATAATCAT21700.16413033612682662No Hit
GACTTGGTGTTCTTCATGAAGCTGACATCCTCCGTCTTATCAAAGAGCAG20970.15860890085620066No Hit
GTGTTAATACTTGGGCATTAATCTTCAGGCACCTCCAGTGAGGCAAATGA20830.157549995461834No Hit
CTATATCATCCAGTGTGCAGTGTTGTGTATTTTTCTAAGCATGACAACAT20420.15444891537833177No Hit
TTCTTGGCCACTTCGTTCAGGAAGAGCTTGGAGTTCAGGTGGCCGATCTT20210.15286055728678186No Hit
GCCTATCAACGTCCTGGTCTCGAACGACCCTTCAGGGAGATCAAGTCTCC19820.1499107494024748No Hit
GGACATAGTGATCCGGTGGTTCTGTATGGAAGGGCCATCGCTCAACGGAT19170.14499440292862978No Hit
CTATACAACTGAAGAATTGAAGGGGGGGGACACCACCAAGAACTCTTCCT18920.14310350043868938No Hit
GTTCTGGACTACAGCCTTGTCTTATGGTCAAATTGATACCCTTAATAAGA18590.14060750915196804No Hit
AACTATATCATCCAGTGTGCAGTGTTGTGTATTTTTCTAAGCATGACAAC17860.13508607388134208No Hit
GTTTGAAATTGATCCCTTATTTTACATGAAATAAAAACAATTTCTGTTGC17750.1342540767857683No Hit
CTCCTAGGGTGAGAGGCTTAGCCATCCCTGACCCTGGCAGTGCACTGGTA17690.1338002601881826No Hit
GGCAATTAGTACTGGTTAGCTGAACGCATTACTGCGCTTCCACACCCAGC17630.1333464435905969No Hit
GTGTGTATCAGGCACAAGGCAGACCCAGCCGGAAACATACCGGTTATAGG17150.12971591080991135No Hit
GTTCAGGAAGAGCTTGGAGTTCAGGTGGCCGATCTTAGTGGGGGAAAGGC17110.12941336641152087No Hit
ATATGAGAGTTTCCTCCAGAGCCATCTTCCAAAGTGGCAGACTTGGCCAA17090.12926209421232565No Hit
ATCTTAGCTTAACGAAACTAAGTAATTTTTGTTTATGATTATAGAACTGC16940.1281275527183614No Hit
GTAAAAATAATAGTGATCAGGCAGAAAAGAAAAATGGAACATCTAAAAAT16830.12729555562278763No Hit
AGCTAAGATTGTGTTTGTGTTAACTTCGACATCAAGGAGCAAAGAACTTT16720.1264635585272139No Hit
CCCCAGAGGAGTGTTCTGGACTACAGCCTTGTCTTATGGTCAAATTGATA16180.12237920914894261No Hit
GTTCTAAAGTTCTTTGCTCCTTGATGTCGAAGTTAACACAAACACAATCT16170.122303573049345No Hit
TCTAAAAATGTATGTGCTAACTATATCATCCAGTGTGCAGTGTTGTGTAT16160.12222793694974737No Hit
GTACATAGCTACCCTGCGATGCCTCTGGCGAGACAACAGGTACACCAGCG16100.12177412035216167No Hit
GTGCTGAAGGGGCCTTCTAGCATTTTCCGCAGCTGCTTCTCCTTCTTGGC16090.12169848425256406No Hit
GATAAAAGGTACTCTGGGGATAACAGGCTGATACCGCCCAAGAGTTCATA16050.1213959398541736No Hit
GTTGTGTATTTTTCTAAGCATGACAACATTGATGTGCCTTTTCAGTGTAA15990.12094212325658792No Hit
GTGGAGGTCCTAGACAAGGCCCAGGCCAAGTTCTGCAGCGAGAACGCAGC15870.12003449006141652No Hit
ATCTAAAAATGTATGTGCTAACTATATCATCCAGTGTGCAGTGTTGTGTA15820.11965630956342842No Hit
CCCTATAACTACTATACAACTGAAGAATTGAAGGGGGGGGACACCACCAA15720.11889994856745228No Hit
GATCAAGGGCGACCTGCTGCTGTTCCAACTGATCGGCTATGCCGTTCTCG15620.11814358757147611No Hit
ATTCCACACTGCACACTCCAGTCATATCTCTTAACAAAATGACATAAATT15580.11784104317308565No Hit
GGGCACCGGCGTGCTGAAGGGGCCTTCTAGCATTTTCCGCAGCTGCTTCT15380.11632832118113332No Hit
GTATATGAGTGCATTCTCGTAAGAATGAAGACAGTGAGGGGTTGGGGGAA15380.11632832118113332No Hit
GTCCTGTTCGGGATGGGAAGGGGTGGTACCGACTCGCTATGGTCACCAGG15350.11610141288234048No Hit
GTTCTGTATGGAAGGGCCATCGCTCAACGGATAAAAGGTACTCTGGGGAT15320.11587450458354763No Hit
GGGTAGCTATGTACGGAAGAGATAACCGCTGAAAGCATCTAAGCGGGAAA15290.1156475962847548No Hit
TAGCTACCCTGCGATGCCTCTGGCGAGACAACAGGTACACCAGCGGTACG15100.1142105103924001No Hit
GTCTTATGGTCAAATTGATACCCTTAATAAGAAAGGAAAGGAAAGGAAAA15020.11360542159561916No Hit
ATCATCCAGTGTGCAGTGTTGTGTATTTTTCTAAGCATGACAACATTGAT15010.11352978549602154No Hit
CTTCAAACACACTGCACTTAGAAACCAGACTTGAAATAATCGAAGCCCCA14820.11209269960366684No Hit
GTTTTGAGGGAGAGCCTGGCGATGACCTACTTTCACACGGGAATCCGCAC14600.11042870541251928No Hit
GTATATAGAGAGCTTCACCCAGGGCTCCATCGAGGCCCACAAGAGGGGCT14530.10989925271533599No Hit
GCTATGTACGGAAGAGATAACCGCTGAAAGCATCTAAGCGGGAAACTTGC14450.10929416391855505No Hit
GGGGATAACAGGCTGATACCGCCCAAGAGTTCATATCGACGGCGGTGTTT14310.10823525852418842No Hit
TCCACACCCAGCCTATCAACGTCCTGGTCTCGAACGACCCTTCAGGGAGA14100.1066469004326385No Hit
TGATTATAGAACTGCAGGATTCACAAGTAGTATTAGAGACATTCCACACT14060.10634435603424802No Hit
GGACATTGAGGACCAGCTGTACAAACTCGAGTCAGACAAGCGCCTGGTGG14010.10596617553625995No Hit
CCCTGAAGGGTCGTTCGAGACCAGGACGTTGATAGGCTGGGTGTGGAAGC13890.10505854234108855No Hit
GTATCTATCCATCTATGTATACACACAGACACTGGGAAAGGATCCTTACG13730.10384836474752669No Hit
GCAGGAAGGGCACCGGCGTGCTGAAGGGGCCTTCTAGCATTTTCCGCAGC13680.10347018424953862No Hit
CTTTAGAACAGACTCCTCAATCTTGTGACTTTCTTATTCTCTAGGAAAGT13580.10271382325356246No Hit
GAATAAGAAAGTCACAAGATTGAGGAGTCTGTTCTAAAGTTCTTTGCTCC13540.102411278855172No Hit
GACAAGCGCCTGGTGGAGGAGAAAGTGAACCAACTGAAGGAGGAAGTTCG13390.10127673736120776No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCATAA151.2271547E-4145.0871145
TTAGATT100.0070783976145.0871145
TGACGGT100.0070783976145.0871145
TGCTTAA1650.0136.29395145
ATGCGGG403.274181E-11126.95121145
TACCGGG600.0120.905914145
CGTTGAA203.857109E-4108.81532145
GAGCGAA1050.096.556461
ATAATAG16850.093.268563
AATAGTG17900.088.208775
TAATAGT17900.087.800834
GACTCCC502.2597305E-887.05225145
AGTCTAA502.2861059E-886.907382
GAGTCTA608.367351E-1084.48691
ATACCTT1200.078.461034
TACGCCC651.5570549E-978.12382145
AGACGCC903.6379788E-1272.54355145
TTACACC300.001931273872.54355145
CGTCAAA300.001931273872.54355145
GCCGTCC406.6441804E-572.54355145
Produced by FastQC (version 0.11.5)