FastQCFastQC Report
Thu 11 Jul 2019
HWC5JBCX2_l01_n02_SM2_268_P1_DM2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWC5JBCX2_l01_n02_SM2_268_P1_DM2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1326875
Sequences flagged as poor quality0
Sequence length151
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAATAATAGTGATCAGGCAGAAAAGAAAAATGGAACATCTAAAAATGTAT101750.766839378238342No Hit
TTATAGAACTGCAGGATTCACAAGTAGTATTAGAGACATTCCACACTGCA66530.501403674046161No Hit
GTCCTAGACAAGGCCCAGGCCAAGTTCTGCAGCGAGAACGCAGCGCAGGC53420.4026000942063119No Hit
ATCTTAGTGGGGGAAAGGCTGCTGCTCGTGCAGGTCTGGGTCCTGTCCAT43110.3248987282147904No Hit
ATATCATCCAGTGTGCAGTGTTGTGTATTTTTCTAAGCATGACAACATTG39940.3010080075365049No Hit
CTCCAGTCATATCTCTTAACAAAATGACATAAATTGACAATGTTCACTAA39320.2963353744700895No Hit
GTATTAGAGACATTCCACACTGCACACTCCAGTCATATCTCTTAACAAAA38610.2909844559585492No Hit
GTACAAACTCGAGTCAGACAAGCGCCTGGTGGAGGAGAAAGTGAACCAAC37450.28224211022138485No Hit
GTATATGGTATTGGGGGCAGGGAGAACCAGAAACCCTCAAAGAGAACCAA36680.2764390014130947No Hit
GGTCTGGGTCCTGTCCATCAGGATTTTCACAGACTTGGTGTTCTTCATGA35520.2676966556759303No Hit
GGTCTAAAGAACGCACTTGAAAGTTGCAAAATTACTTGCCAATGTTTGGG35180.26513424399434765No Hit
GTATGGAAGGGCCATCGCTCAACGGATAAAAGGTACTCTGGGGATAACAG32790.24712199717381064No Hit
GGGTATGGCTGTTCGCCATTTAAAGAGGTACGTGAGCTGGGTTTAAAACG32680.24629298162976918No Hit
CTACTATACAACTGAAGAATTGAAGGGGGGGGACACCACCAAGAACTCTT28980.21840791333019313No Hit
GTGCAGTGTTGTGTATTTTTCTAAGCATGACAACATTGATGTGCCTTTTC28040.21132359868111164No Hit
CTCTAATACTACTTGTGAATCCTGCAGTTCTATAATCATAAACAAAAATT26810.20205369759773903No Hit
GTATTGGGGGCAGGGAGAACCAGAAACCCTCAAAGAGAACCAATTTGTAG26480.19956665096561468No Hit
TGACTGGAGTGTGCAGTGTGGAATGTCTCTAATACTACTTGTGAATCCTG25680.19353744700894962No Hit
GTGGAGGAGAAAGTGAACCAACTGAAGGAGGAAGTTCGGCTGCAGTACGA25550.1925577013659915No Hit
GGCTGATACCGCCCAAGAGTTCATATCGACGGCGGTGTTTGGCACCTCGA25440.19172868582195007No Hit
GCTGTACAAACTCGAGTCAGACAAGCGCCTGGTGGAGGAGAAAGTGAACC25380.19127649552520018No Hit
TTCTAGCATTTTCCGCAGCTGCTTCTCCTTCTTGGCCACTTCGTTCAGGA25310.190748940178992No Hit
GTACGAGAAGCTGCACCAGCTGCTGGACGAGGACCTGCGGCAGACAGTGG24320.18328780028261893No Hit
GTTCTATAATCATAAACAAAAATTACTTAGTTTCGTTAAGCTAAGATTGT24240.18268487988695242No Hit
AAGCAGGACATTGAGGACCAGCTGTACAAACTCGAGTCAGACAAGCGCCT23620.17801224682053698No Hit
GGCTATGACTTGTCGCGTCGCTGACTCGATGGTCAACGCCGCCAATATGG22740.17138012246820536No Hit
GTCCCAAGGGTATGGCTGTTCGCCATTTAAAGAGGTACGTGAGCTGGGTT22660.17077720207253885No Hit
ATTGAGGACCAGCTGTACAAACTCGAGTCAGACAAGCGCCTGGTGGAGGA22370.16859161563824776No Hit
ACATTGAGGACCAGCTGTACAAACTCGAGTCAGACAAGCGCCTGGTGGAG22210.16738577484691475No Hit
GTGTGGAATGTCTCTAATACTACTTGTGAATCCTGCAGTTCTATAATCAT21770.16406971267074894No Hit
GACTTGGTGTTCTTCATGAAGCTGACATCCTCCGTCTTATCAAAGAGCAG21020.15841733396137542No Hit
GTGTTAATACTTGGGCATTAATCTTCAGGCACCTCCAGTGAGGCAAATGA20890.15743758831841734No Hit
CTATATCATCCAGTGTGCAGTGTTGTGTATTTTTCTAAGCATGACAACAT20470.15427225624116817No Hit
TTCTTGGCCACTTCGTTCAGGAAGAGCTTGGAGTTCAGGTGGCCGATCTT20270.15276495525200187No Hit
GCCTATCAACGTCCTGGTCTCGAACGACCCTTCAGGGAGATCAAGTCTCC19840.14952425812529438No Hit
GGACATAGTGATCCGGTGGTTCTGTATGGAAGGGCCATCGCTCAACGGAT19220.14485162505887894No Hit
CTATACAACTGAAGAATTGAAGGGGGGGGACACCACCAAGAACTCTTCCT18940.14274140367404617No Hit
GTTCTGGACTACAGCCTTGTCTTATGGTCAAATTGATACCCTTAATAAGA18600.1401789919924635No Hit
AACTATATCATCCAGTGTGCAGTGTTGTGTATTTTTCTAAGCATGACAAC17930.1351295336787565No Hit
GTTTGAAATTGATCCCTTATTTTACATGAAATAAAAACAATTTCTGTTGC17790.1340744229863401No Hit
CTCCTAGGGTGAGAGGCTTAGCCATCCCTGACCCTGGCAGTGCACTGGTA17740.13369759773904852No Hit
GGCAATTAGTACTGGTTAGCTGAACGCATTACTGCGCTTCCACACCCAGC17660.13309467734338198No Hit
GTGTGTATCAGGCACAAGGCAGACCCAGCCGGAAACATACCGGTTATAGG17210.12970325011775788No Hit
GTTCAGGAAGAGCTTGGAGTTCAGGTGGCCGATCTTAGTGGGGGAAAGGC17170.12940178991992463No Hit
ATATGAGAGTTTCCTCCAGAGCCATCTTCCAAAGTGGCAGACTTGGCCAA17140.1291756947715497No Hit
ATCTTAGCTTAACGAAACTAAGTAATTTTTGTTTATGATTATAGAACTGC16950.12774375883184175No Hit
GTAAAAATAATAGTGATCAGGCAGAAAAGAAAAATGGAACATCTAAAAAT16870.1271408384361752No Hit
AGCTAAGATTGTGTTTGTGTTAACTTCGACATCAAGGAGCAAAGAACTTT16780.12646255299105041No Hit
CCCCAGAGGAGTGTTCTGGACTACAGCCTTGTCTTATGGTCAAATTGATA16210.12216674517192651No Hit
TCTAAAAATGTATGTGCTAACTATATCATCCAGTGTGCAGTGTTGTGTAT16210.12216674517192651No Hit
GTTCTAAAGTTCTTTGCTCCTTGATGTCGAAGTTAACACAAACACAATCT16200.1220913801224682No Hit
GTACATAGCTACCCTGCGATGCCTCTGGCGAGACAACAGGTACACCAGCG16140.12163918982571834No Hit
GTGCTGAAGGGGCCTTCTAGCATTTTCCGCAGCTGCTTCTCCTTCTTGGC16130.12156382477626002No Hit
GATAAAAGGTACTCTGGGGATAACAGGCTGATACCGCCCAAGAGTTCATA16110.12141309467734339No Hit
GTTGTGTATTTTTCTAAGCATGACAACATTGATGTGCCTTTTCAGTGTAA16000.12058407913330194No Hit
GTGGAGGTCCTAGACAAGGCCCAGGCCAAGTTCTGCAGCGAGAACGCAGC15890.11975506358926048No Hit
ATCTAAAAATGTATGTGCTAACTATATCATCCAGTGTGCAGTGTTGTGTA15850.11945360339142722No Hit
CCCTATAACTACTATACAACTGAAGAATTGAAGGGGGGGGACACCACCAA15750.11869995289684408No Hit
GATCAAGGGCGACCTGCTGCTGTTCCAACTGATCGGCTATGCCGTTCTCG15630.11779557230334432No Hit
ATTCCACACTGCACACTCCAGTCATATCTCTTAACAAAATGACATAAATT15610.1176448422044277No Hit
GGGCACCGGCGTGCTGAAGGGGCCTTCTAGCATTTTCCGCAGCTGCTTCT15470.11658973151201131No Hit
GTATATGAGTGCATTCTCGTAAGAATGAAGACAGTGAGGGGTTGGGGGAA15390.11598681111634479No Hit
GTCCTGTTCGGGATGGGAAGGGGTGGTACCGACTCGCTATGGTCACCAGG15390.11598681111634479No Hit
GTTCTGTATGGAAGGGCCATCGCTCAACGGATAAAAGGTACTCTGGGGAT15330.11553462081959492No Hit
GGGTAGCTATGTACGGAAGAGATAACCGCTGAAAGCATCTAAGCGGGAAA15320.1154592557701366No Hit
TAGCTACCCTGCGATGCCTCTGGCGAGACAACAGGTACACCAGCGGTACG15140.11410268487988695No Hit
GTCTTATGGTCAAATTGATACCCTTAATAAGAAAGGAAAGGAAAGGAAAA15060.11349976448422044No Hit
ATCATCCAGTGTGCAGTGTTGTGTATTTTTCTAAGCATGACAACATTGAT15050.11342439943476212No Hit
CTTCAAACACACTGCACTTAGAAACCAGACTTGAAATAATCGAAGCCCCA14850.11191709844559586No Hit
GTTTTGAGGGAGAGCCTGGCGATGACCTACTTTCACACGGGAATCCGCAC14630.11025906735751295No Hit
GTATATAGAGAGCTTCACCCAGGGCTCCATCGAGGCCCACAAGAGGGGCT14560.10973151201130475No Hit
GCTATGTACGGAAGAGATAACCGCTGAAAGCATCTAAGCGGGAAACTTGC14500.10927932171455489No Hit
GGGGATAACAGGCTGATACCGCCCAAGAGTTCATATCGACGGCGGTGTTT14310.10784738577484691No Hit
TCCACACCCAGCCTATCAACGTCCTGGTCTCGAACGACCCTTCAGGGAGA14140.10656617993405558No Hit
TGATTATAGAACTGCAGGATTCACAAGTAGTATTAGAGACATTCCACACT14080.1061139896373057No Hit
GGACATTGAGGACCAGCTGTACAAACTCGAGTCAGACAAGCGCCTGGTGG14060.10596325953838906No Hit
CCCTGAAGGGTCGTTCGAGACCAGGACGTTGATAGGCTGGGTGTGGAAGC13930.104983513895431No Hit
GTATCTATCCATCTATGTATACACACAGACACTGGGAAAGGATCCTTACG13770.10377767310409797No Hit
GCAGGAAGGGCACCGGCGTGCTGAAGGGGCCTTCTAGCATTTTCCGCAGC13700.1032501177578898No Hit
GAATAAGAAAGTCACAAGATTGAGGAGTCTGTTCTAAAGTTCTTTGCTCC13580.10234573716439001No Hit
CTTTAGAACAGACTCCTCAATCTTGTGACTTTCTTATTCTCTAGGAAAGT13580.10234573716439001No Hit
GACAAGCGCCTGGTGGAGGAGAAAGTGAACCAACTGAAGGAGGAAGTTCG13410.10106453132359867No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCGAAA100.0070748264145.1116145
TGCTTAA1250.0127.69822145
GAGCGAA1000.0115.8530651
ATGCGGG404.791218E-9108.83371145
TCGACCC203.8545206E-4108.83371145
TACGCCC301.5935511E-596.74108145
GTCATAA406.090613E-790.694756145
ATAATAG17750.089.33743
TTCGCCC259.358773E-487.06697145
CGTCAAA259.358773E-487.06697145
TAATAGT18500.085.324224
AATAGTG18600.084.875085
TACCGGG950.084.01199145
CGGTGAA353.4561388E-582.7677847
AGTGAAA554.372123E-879.151794145
CGTCGCC554.372123E-879.151794145
GACTCCC755.4569682E-1177.39286145
ACGCCCC502.296134E-672.55581145
GTTAGCC607.991002E-872.55581145
AGACGCC607.991002E-872.55581145
Produced by FastQC (version 0.11.5)