FastQCFastQC Report
Thu 11 Jul 2019
HWC5JBCX2_l01_n02_SM2_240_P1_DM2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWC5JBCX2_l01_n02_SM2_240_P1_DM2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2062776
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTACTATACTGTCTCATGGTCATGTACCTGAATCCTGGAAATTATTCAAT39240.19022908934368055No Hit
TCATAGATGTGCCCGTACAGGCAGCAATTTCAACAACATTCCCATACACT36750.17815797740520542No Hit
ATTATTATGTGGTCATTCAATACCTCCATGTTGTCAGAGAGCACTTCCTT35340.17132252847618937No Hit
GAATACAACAATAACCTCTTTTAGGTTGAATGATTTAAATGGAGCCGACA35280.1710316583089972No Hit
CTGTAGAACATGCTTGTAAATTCAAACATTCCAGTCTCATTACAGTAACT34280.16618382218912764No Hit
GTTTAGTATACCATTAGAAAGATATAATGAAGAAACAAGGGCAAAATTGA31110.15081618168914124No Hit
GTAAGAAATACAGTAAAATTGAATTTAATGCAATTCTTCTATCTCCAAAA30450.14761660985002734No Hit
GTATAGTATGATTATCCAATCCATCGTCATTTAAAGATGCAGCAGTAATA29730.1441261678437213No Hit
GTATAGTAGCGCTGAGCTTTCATGGCCTTCTGCTATTTCAAATGCTTCAT29650.1437383409541317No Hit
GCTTATATGTACCCAATCTCACCAAGGTGAGCCATTGCTTTCTCAAAATC28370.1375331107206987No Hit
GTATAGCACCGGTCCTGTTCTCCAATAAGATAGCAAGATTGGGGAAAGGA27770.13462440904877698No Hit
GTTGTATTCAGTGCCGTTGCAGCAGGTTGGTTTCTGCATACTGTCCAATC27740.1344789739651809No Hit
TTCTTAATCTGTGGGCCATAGCCTCAAGCATGCTATGTTGACCCACTGGC27230.13200657754404743No Hit
GTGTAATACCACTGACAACAACACCCACCAAATCTCATTTTGCAAATCTC27220.13195809918284876No Hit
CCCATACACTGGTGTTCCCCCTTATTCCCATGGAACAGGAACAGGTTACA26380.12788591684215833No Hit
GTTCTACAGAGATGGATTTGTATCTAATTTTGCAATGGAACTCCCTTCGT25850.12531656369862748No Hit
CCATTAGAAAGATATAATGAAGAAACAAGGGCAAAATTGAAGAAGCTAAA24110.11688132885005449No Hit
GTTTAATATGCTATCTACCGTGTTGGGAGTAGCTGCACTAGGTATCAAGA23560.11421501898412625No Hit
GTGCTATACTACAAAAGTCCCTGAACCAAACTGGGCTGTCTCTGGTTATT23430.1135848002885432No Hit
ATAATAAACAGTGAAGATGAACATCTCTTGGCGCTTGAGAGAAAGCTGAA22800.1105306635330254No Hit
TCTATATGCTTTTTAAAACCCAAAGACCAGGAAAGAAAAAGAAGATTCAT22570.10941566122545542No Hit
ATTCAATGATGACCAATAACCCCATAAACATCTTCGAAGCTTATATGTAC22510.10912479105826324No Hit
GTTAAAACAGGCCTCAAATAGGTTGCAACATTTAGCGTAGCATTGTTCCT22500.10907631269706454No Hit
GTATAGATCTGTTTCTTTTGGTTCTCCAACTATGAGTTCCAGACACCGCA21000.10180455851726024No Hit
CTGTTAAAACAGGCCTCAAATAGGTTGCAACATTTAGCGTAGCATTGTTC20870.10117433982167719No Hit
AGATTATACTTCAATACTATTTCTGGGATATGCAAGTTTCTCAAATTGTA20630.1000108591529085No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAAACAC7750.0103.765484
AAACACG8350.096.313945
AGCACGC2850.094.049581
GTAACAA10400.094.044214
CACGCAC3100.091.140943
ACACGAG6600.090.025337
GCACGCA3000.089.347112
GGTACCC900.088.65393145
AACACGA6900.086.10916
TAGAAAC10050.085.058162
AGTAGCC1150.081.99613145
CACGAGC7550.078.697648
AACACGT2700.077.824896
ACACGTG2800.075.047257
ACGAGCA4550.071.6631559
CAACGCA3100.070.122019
AGTGGGG754.840331E-967.69936145
TTCGTCC651.394128E-766.95542145
GCCGACC902.8194336E-1064.475586145
ACGTGCA1850.062.6676039
Produced by FastQC (version 0.11.5)