FastQCFastQC Report
Thu 11 Jul 2019
HWC5JBCX2_l01_n02_SM2_231_P1_DM2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWC5JBCX2_l01_n02_SM2_231_P1_DM2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1966134
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGCACGCACTTTCTTAAAATGTCGCTGTTTGGAGACACAATTGCCTACTT60600.30821907357280837No Hit
CTACTATACTGTCTCATGGTCATGTACCTGAATCCTGGAAATTATTCAAT48640.24738903859045214No Hit
GTATAGTAGCGCTGAGCTTTCATGGCCTTCTGCTATTTCAAATGCTTCAT42190.21458354313592054No Hit
ATTATTATGTGGTCATTCAATACCTCCATGTTGTCAGAGAGCACTTCCTT37850.19250976789984814No Hit
GTAAGAAATACAGTAAAATTGAATTTAATGCAATTCTTCTATCTCCAAAA34020.17302991556018055No Hit
TCTATATGCTTTTTAAAACCCAAAGACCAGGAAAGAAAAAGAAGATTCAT26890.13676585624377585No Hit
CTGTAGAACATGCTTGTAAATTCAAACATTCCAGTCTCATTACAGTAACT25900.13173059415075472No Hit
TCATAGATGTGCCCGTACAGGCAGCAATTTCAACAACATTCCCATACACT25800.13122198181812633No Hit
GTTGTATTCAGTGCCGTTGCAGCAGGTTGGTTTCTGCATACTGTCCAATC24640.12532207875963694No Hit
GTCTCATGGTCATGTACCTGAATCCTGGAAATTATTCAATGCAAGTAAAA24450.12435571532764297No Hit
GTATAGTATGATTATCCAATCCATCGTCATTTAAAGATGCAGCAGTAATA24180.1229824620295463No Hit
GTGTGAATGTGATGCTTGTTTCTCGCATAAAGCACAGAGCGTTCCTAGTT24130.12272815586323212No Hit
TTCTTAATCTGTGGGCCATAGCCTCAAGCATGCTATGTTGACCCACTGGC23890.12150748626492396No Hit
GTGTAATACCACTGACAACAACACCCACCAAATCTCATTTTGCAAATCTC23700.12054112283293002No Hit
CATTCACACAGGGCTCATAGCAGAGCAGCGAGATCTTCAGTGCCTGGAGT23480.11942217570114753No Hit
GAATACAACAATAACCTCTTTTAGGTTGAATGATTTAAATGGAGCCGACA23150.11774375500347381No Hit
GTTCATAGCTGAGACCATCTGCATTTCTCGTCTCACTCCAGGCACTGAAG22830.11611619553906297No Hit
GCCTTGGAATGGATAAAAAACAAAAGATGCTTAACTGATATACAAAAAGC22370.11377657880897232No Hit
CCCATACACTGGTGTTCCCCCTTATTCCCATGGAACAGGAACAGGTTACA22260.1132171052430811No Hit
GTTTATTGTCTCTTTGTTTGGACCTTTTATTCGTATTTTCATGTTTATTC22230.11306452154329258No Hit
GCTTTAGCACTTCCATTACATCCTTTGCAATCCCTTCCCCATTCTTTTGA22120.11250504797740134No Hit
GTTCTACAGAGATGGATTTGTATCTAATTTTGCAATGGAACTCCCTTCGT21580.109758541381208No Hit
TTATAGATATTTCTTCACAAGAGCTGAATTTCCCATGGAGCTCTGCTTTA21270.10818184315005996No Hit
GCTATGAACACAGCAAAAACAATGAATGGAATGGGAAAAGGAGAAGACGT21170.10767323081743156No Hit
GCTATGAGCCCTGTGTGAATGTGATGCTTGTTTCTCGCATAAAGCACAGA21050.1070628960182775No Hit
GCTTATATGTACCCAATCTCACCAAGGTGAGCCATTGCTTTCTCAAAATC20760.10558792025365514No Hit
GTTTAGTATACCATTAGAAAGATATAATGAAGAAACAAGGGCAAAATTGA20370.1036043321564044No Hit
GTAGTAACAAGAGCCTTTTTTCATTTTAATCATTTGTGTATCGCATGTAT20120.10233280132483341No Hit
CAATTATTATGTGGTCATTCAATACCTCCATGTTGTCAGAGAGCACTTCC19880.10111213172652525No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACGCA8050.0125.064852
CACGCAC8200.0122.777083
AGCACGC8350.0121.438931
CGGTGCA1050.0110.368982
GTAACAA12500.0103.721894
GAAACAC7350.097.560784
AAACACG7650.092.7951355
ACACGTG2500.092.724097
ACGCACT11500.087.547624
CGCACTT11600.086.175075
CGCACCC608.258212E-1084.64764145
GGTACCC554.373578E-879.15104145
AACACGT2950.078.577746
CAGCGCC651.5552359E-978.13628145
TAGAAAC10450.076.241742
AGTAGCC1150.075.70969145
ACGTGCA2500.075.326832
AGTGGGG502.296676E-672.55512145
ATTGCGG502.296676E-672.55512145
AACACGA6600.072.438856
Produced by FastQC (version 0.11.5)