FastQCFastQC Report
Tue 2 Jul 2019
HWC5JBCX2_l01_n02_SM2_2017_P1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWC5JBCX2_l01_n02_SM2_2017_P1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1585115
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTATAGAACATGCTTGTAAATTCAAACATTCCAGTTTCGTTACAGTAACT43870.27676225384278114No Hit
GTAAGAAATACAGTAAAAATTGAATTTAATGCGATTCTTCTACCTCCAAA30070.18970232443702822No Hit
GTATATGCTTCTCCATATTTTATTTTGAGCAATGCATTACTGTCTGGGCC28890.18225806960378269No Hit
CTTTACATACAGTCCCATCCCCATTCTTTTAGTTTTAGACATCGTCCGAG27200.1715963825968463No Hit
CTTCTGTATTGTTCCGTTCCCGATTAATATTTCTTCATCATTTTTGAATC26080.16453064919579968No Hit
ATATAGTAGCGCTGAGCTTTCATGGCCTTCTGCTATTTCAAATGCTTCAT26060.1644044753850667No Hit
GTTCTATAGAGATGGATTTGTATCTAACTTTGCAATGGAAATTCCTTCAT24930.1572756550786536No Hit
CTACTATATTGTCTCATGGTCATGTACCTGAATCCTGGAAATTATTCAAT24870.15689713364645466No Hit
GTATTTACACAGTCTCTCCAAAGTTTCCATAGGAAATTTTCGTTAGCTTT23920.15090387763663837No Hit
ATGCTACACTGCTTACAAGGTGAAAATTGGAGGCAAATATATCACCCGGG23200.14636162045025125No Hit
CATTATAGGTATTTCTTCACAAGAGCTGAATTTCCCATAGAGCTCTGCTT23150.1460461859234188No Hit
ATATTACTCTTAGTGCTTTTCCAAAGGATCCTTTTACTAGTTTCCCTTCT22730.1433965358980263No Hit
GTATTGTTCCGTTCCCGATTAATATTTCTTCATCATTTTTGAATCCTCTT22620.14270257993899496No Hit
ATGCATTACTGTCTGGGCCATCAACTCCGATGTAAGTCCATTCTCTACCA22530.14213479779069657No Hit
GCTTTAGCACTTCCATTACATCCTTTGCAATTCCTTCCCCATTCTTCTGA22350.1409992334940998No Hit
TTCTAATGGTATGCTGAACAGATCAGGACAAGGTATTTGAGCCTTTAGTC20460.12907580837983365No Hit
TACCATTCCTATGCAAAAAACATCCTAAGGACACAAGAAAGTGCCTGCAA20280.12794024408323687No Hit
GTCTATAGCTGTCAGACATGATTTTAAAGGTTCAGTATCTATCACGGTCT19970.12598455001687575No Hit
GTCATAATGGGAGCCAATGACGTAAGTGAATTAGAATCACAAGCACAACT19940.1257952893007763No Hit
ACTATATTGTCTCATGGTCATGTACCTGAATCCTGGAAATTATTCAATGC19830.12510133334174492No Hit
GCATTACTGTCTGGGCCATCAACTCCGATGTAAGTCCATTCTCTACCATC18980.11973894638559346No Hit
CATATGGACCAATAGGAGATACTGAAGGTTTCGAAAAAGTCTACGAAAGC18690.11790942612996533No Hit
GCACTATATCTTTTCCTTTTAACTACTTTAACGGGTTTTCCTTGATAAAG18510.11677386183336856No Hit
GTAATGCATTGCTCAAAATAAAATATGGAGAAGCATATACTGACACATAC18480.1165846011172691No Hit
GGAATGGTATGTGTCAGTATATGCTTCTCCATATTTTATTTTGAGCAATG18410.11614299277970368No Hit
CTATATGTGTCAGCTTCATTGTCATACTTACTATATTCGGATATATTGCT18390.11601681896897072No Hit
CTCCTAATTAGCCCCCATAGATTCGGAGAAACCAAAGGAAACTCAGCTCC18290.11538594991530582No Hit
ATATTGTCTCATGGTCATGTACCTGAATCCTGGAAATTATTCAATGCAAG18080.11406112490260958No Hit
CCCCCAGACCAAATGATGGAAGCATAACAGGGCCTTGCGAATCTGATGGG17930.11311482132211229No Hit
GGATAAAGCACTATATCTTTTCCTTTTAACTACTTTAACGGGTTTTCCTT17530.11059134510745278No Hit
TTATTATTGTCATTCCTATTGCCATATCTGCTGATTCATTTACTCCAGCA17510.1104651712967198No Hit
GTGTAAATACAATAAGTAATGAGGAAATGAGTAACGAGTTACAGAAAACC17410.10983430224305493No Hit
GTGTAGCATTTCTATGAACTCAGCTGATGTTGCTCCTGCCACGGGCAGGA17230.10869873794645815No Hit
AGCATATACTGACACATACCATTCCTATGCAAAAAACATCCTAAGGACAC17180.10838330341962571No Hit
GTTCATAGCTGAGACCATCTGCATTTCCCGTCTCACTCCTGGCACTGAGG17120.10800478198742679No Hit
GTCCCATCCCCATTCTTTTAGTTTTAGACATCGTCCGAGAGTACCACCTT17100.1078786081766938No Hit
GTACTGAAGGGAGGAGGAGAAAATTTCATCGAAGTAAGGAAAGGGTCCCC16770.10579674029959972No Hit
GTTCATAGAAATGCTACACTGCTTACAAGGTGAAAATTGGAGGCAAATAT16670.10516587124593485No Hit
TCCTAATGCAGCTCTTATTATGTCACAGGCGACATCACCTCCGTCTATAG16480.10396722004397158No Hit
GTGTGAATGTGATGCTTGTTTTTCGCACAAAGCACAGAGCGTTCCTAGTT16110.10163300454541152No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGCACCC100.007074116145.11737145
CCTCGGA100.007074116145.11737145
GCCGACC100.007074116145.11737145
TAACACG100.0071116127144.861015
ATAAGCC256.4400883E-6116.0939145
GAACGCC203.854052E-4108.838036145
GAAACAC2550.0107.932244
ACGTGCA950.0106.7598959
AACACGT1300.0105.866646
GAGCAGA1050.0103.459091
ACGAGCC650.0100.307379
AAACACG2800.098.298535
AACACGA1550.098.137846
CACGTGC1450.094.9209068
ACACGAG1550.093.467567
GTACCCC406.089722E-790.69836145
CACGAGC1650.087.8056268
CAACGCA1950.085.447029
ACAACGC1950.085.441638
ACACGTG1650.083.412717
Produced by FastQC (version 0.11.5)