FastQCFastQC Report
Thu 11 Jul 2019
HWC5JBCX2_l01_n02_SM2_2017_P1_DM2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWC5JBCX2_l01_n02_SM2_2017_P1_DM2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1591460
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTATAGAACATGCTTGTAAATTCAAACATTCCAGTTTCGTTACAGTAACT43990.27641285360612267No Hit
GTAAGAAATACAGTAAAAATTGAATTTAATGCGATTCTTCTACCTCCAAA30130.1893230115742777No Hit
GTATATGCTTCTCCATATTTTATTTTGAGCAATGCATTACTGTCTGGGCC28950.18190843627863723No Hit
CTTTACATACAGTCCCATCCCCATTCTTTTAGTTTTAGACATCGTCCGAG27310.17160343332537417No Hit
ATATAGTAGCGCTGAGCTTTCATGGCCTTCTGCTATTTCAAATGCTTCAT26140.16425169341359505No Hit
CTTCTGTATTGTTCCGTTCCCGATTAATATTTCTTCATCATTTTTGAATC26130.1641888580297337No Hit
GTTCTATAGAGATGGATTTGTATCTAACTTTGCAATGGAAATTCCTTCAT24980.1569627888856773No Hit
CTACTATATTGTCTCATGGTCATGTACCTGAATCCTGGAAATTATTCAAT24960.1568371181179546No Hit
GTATTTACACAGTCTCTCCAAAGTTTCCATAGGAAATTTTCGTTAGCTTT23980.15067925049954128No Hit
ATGCTACACTGCTTACAAGGTGAAAATTGGAGGCAAATATATCACCCGGG23280.1462807736292461No Hit
CATTATAGGTATTTCTTCACAAGAGCTGAATTTCCCATAGAGCTCTGCTT23210.14584092594221656No Hit
ATATTACTCTTAGTGCTTTTCCAAAGGATCCTTTTACTAGTTTCCCTTCT22750.14295049828459402No Hit
GTATTGTTCCGTTCCCGATTAATATTTCTTCATCATTTTTGAATCCTCTT22690.14257348598142586No Hit
ATGCATTACTGTCTGGGCCATCAACTCCGATGTAAGTCCATTCTCTACCA22580.14188229675895092No Hit
GCTTTAGCACTTCCATTACATCCTTTGCAATTCCTTCCCCATTCTTCTGA22360.14049991831400097No Hit
TTCTAATGGTATGCTGAACAGATCAGGACAAGGTATTTGAGCCTTTAGTC20470.12862403076420392No Hit
TACCATTCCTATGCAAAAAACATCCTAAGGACACAAGAAAGTGCCTGCAA20320.12768150000628353No Hit
GTCTATAGCTGTCAGACATGATTTTAAAGGTTCAGTATCTATCACGGTCT20000.12567076772272No Hit
GTCATAATGGGAGCCAATGACGTAAGTGAATTAGAATCACAAGCACAACT19980.1255450969549973No Hit
ACTATATTGTCTCATGGTCATGTACCTGAATCCTGGAAATTATTCAATGC19880.1249167431163837No Hit
GCATTACTGTCTGGGCCATCAACTCCGATGTAAGTCCATTCTCTACCATC19020.11951290010430674No Hit
CATATGGACCAATAGGAGATACTGAAGGTTTCGAAAAAGTCTACGAAAGC18750.11781634474005002No Hit
GCACTATATCTTTTCCTTTTAACTACTTTAACGGGTTTTCCTTGATAAAG18560.11662247244668418No Hit
GTAATGCATTGCTCAAAATAAAATATGGAGAAGCATATACTGACACATAC18500.11624546014351601No Hit
CTATATGTGTCAGCTTCATTGTCATACTTACTATATTCGGATATATTGCT18440.11586844784034786No Hit
GGAATGGTATGTGTCAGTATATGCTTCTCCATATTTTATTTTGAGCAATG18430.11580561245648649No Hit
CTCCTAATTAGCCCCCATAGATTCGGAGAAACCAAAGGAAACTCAGCTCC18380.1154914355371797No Hit
ATATTGTCTCATGGTCATGTACCTGAATCCTGGAAATTATTCAATGCAAG18100.11373204478906161No Hit
CCCCCAGACCAAATGATGGAAGCATAACAGGGCCTTGCGAATCTGATGGG17990.11304085556658665No Hit
TTATTATTGTCATTCCTATTGCCATATCTGCTGATTCATTTACTCCAGCA17590.11052744021213226No Hit
GGATAAAGCACTATATCTTTTCCTTTTAACTACTTTAACGGGTTTTCCTT17560.11033893406054818No Hit
GTGTAAATACAATAAGTAATGAGGAAATGAGTAACGAGTTACAGAAAACC17450.10964774483807321No Hit
GTGTAGCATTTCTATGAACTCAGCTGATGTTGCTCCTGCCACGGGCAGGA17270.10851670792856874No Hit
AGCATATACTGACACATACCATTCCTATGCAAAAAACATCCTAAGGACAC17220.10820253100926193No Hit
GTTCATAGCTGAGACCATCTGCATTTCCCGTCTCACTCCTGGCACTGAGG17190.10801402485767786No Hit
GTCCCATCCCCATTCTTTTAGTTTTAGACATCGTCCGAGAGTACCACCTT17130.10763701255450969No Hit
GTACTGAAGGGAGGAGGAGAAAATTTCATCGAAGTAAGGAAAGGGTCCCC16810.10562628027094617No Hit
GTTCATAGAAATGCTACACTGCTTACAAGGTGAAAATTGGAGGCAAATAT16680.10480942028074848No Hit
TCCTAATGCAGCTCTTATTATGTCACAGGCGACATCACCTCCGTCTATAG16520.10380405413896673No Hit
GTGTGAATGTGATGCTTGTTTTTCGCACAAAGCACAGAGCGTTCCTAGTT16160.10154198031995776No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACGGCC151.227575E-4145.07602145
TAACACG100.0071074916144.889115
TGACGCC650.0111.59694145
CAGCGCC203.8584272E-4108.80702145
GAGCAGA607.2759576E-1296.574531
AACACGA2100.093.142996
ACACGAG2150.090.979747
GGAGTTT406.100072E-790.672516145
AGCACGC651.6370905E-1189.145721
GAAACAC2800.087.960094
AAACACG2950.083.4954155
TGGAGCC353.4289762E-582.90058145
CAACGCA1250.081.1455469
GAACGCC451.229384E-680.597786145
ACAACGC1300.078.022128
AACAACG1300.078.019667
CACGAGC2550.076.710828
GTACCCC406.647111E-572.53801145
ACGAGCC903.6379788E-1272.4513859
TAGAAAC4200.072.430892
Produced by FastQC (version 0.11.5)