FastQCFastQC Report
Thu 11 Jul 2019
HWC5JBCX2_l01_n02_SM2_2016_P1_DM2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWC5JBCX2_l01_n02_SM2_2016_P1_DM2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1565942
Sequences flagged as poor quality0
Sequence length151
%GC39

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAAGAAATACAGTAAAAATTGAATTTAATGCGATTCTTCTACCTCCAAA150880.9635095041834244No Hit
CCTATAATGTTTGAACCATTCCAGATTCTTTCAATTTGTTCTTTTATTTT81350.5194956135029267No Hit
GATATACAGAAAGCACTAATTGGTGCCTCTATCTGCTTTTTAAAACCCAA74370.47492180425584085No Hit
ATATAGTAGCGCTGAGCTTTCATGGCCTTCTGCTATTTCAAATGCTTCAT65000.4150856161977902No Hit
GAATAAAGGGGCCAAATAAAGAGACAATAAACAGAGAGGTATCAATTTTG63890.40799723105964336No Hit
ATTACTATGTGGTCACTCAATACTTCCATGTTGTCAGAGAGTACTTCCTT60150.3841138432968782No Hit
CTACTATATTGTCTCATGGTCATGTACCTGAATCCTGGAAATTATTCAAT58760.37523739704280235No Hit
CATTATAGGTATTTCTTCACAAGAGCTGAATTTCCCATAGAGCTCTGCTT55610.3551217094886018No Hit
CAATTACTATGTGGTCACTCAATACTTCCATGTTGTCAGAGAGTACTTCC49140.31380472584552943No Hit
GCTTTAGCACTTCCATTACATCCTTTGCAATTCCTTCCCCATTCTTCTGA48510.3097815883346893No Hit
ACTATATTGTCTCATGGTCATGTACCTGAATCCTGGAAATTATTCAATGC47810.3053114355448669No Hit
ATACTATACTGCTCTATTACTCAACTGCTGCTTCTAGTTTGGCTGTAACA47350.30237390656869795No Hit
GTAGTAACAAGAGCATTTTTCAATAACGTTTCTTTGTAATGATGACAAGC47210.30147987601073345No Hit
AGCACGCACTTTCTTAAAATGTCGCTGTTTGGAGACACAATTGCCTACCT46650.29790375377887557No Hit
ATATTGTCTCATGGTCATGTACCTGAATCCTGGAAATTATTCAATGCAAG44720.28557890394407964No Hit
GCCTTGGAATGGATAAAAAACAAAAGATGCTTAACTGATATACAGAAAGC44250.2825775156423418No Hit
GTGTGAATGTGATGCTTGTTTTTCGCACAAAGCACAGAGCGTTCCTAGTT43330.27670245769000384No Hit
AGTAGAAACAACGCACTTTTTCCAGTTTATTTGCTGACATTGATTACAAT42940.2742119439928171No Hit
GTTCATAGCTGAGACCATCTGCATTTCCCGTCTCACTCCTGGCACTGAGG42690.27261546085359484No Hit
CTATAGAACATGCTTGTAAATTCAAACATTCCAGTTTCGTTACAGTAACT42640.2722961642257504No Hit
CATTCACACAGAGCTCATAGCAGAGCAGCGAGATCCTCAGTGCCAGGAGT39650.253202225880652No Hit
GCTATGAGCTCTGTGTGAATGTGATGCTTGTTTTTCGCACAAAGCACAGA35700.22797779228094014No Hit
GTTTATTGTCTCTTTATTTGGCCCCTTTATTCTTATTTTCATGTTTACTC35330.2256149972348912No Hit
AAGAAATACAGTAAAAATTGAATTTAATGCGATTCTTCTACCTCCAAAAC34920.22299676488656667No Hit
CTTCTGACTTGCCCCAAGGGATCTCAATACTCCAATGTTGCTTTGCAGTT34300.21903748670129547No Hit
GTCCTATTGTCCAAGCCATGAAATGGAGAGCTGATAAAATAAAAGAACAA33690.21514206784159312No Hit
GCTATGAACACAGCAAAAACAATGAATGGGATGGGAAAAGGAGAAGACGT33060.211118930330753No Hit
CAATACTTCCATGTTGTCAGAGAGTACTTCCTTCATTGCTTCTTTAGCCT32960.21048033707506408No Hit
ATTACATCCTTTGCAATTCCTTCCCCATTCTTCTGACTTGCCCCAAGGGA31090.19853864319368147No Hit
GTTTGAACCATTCCAGATTCTTTCAATTTGTTCTTTTATTTTATCAGCTC30610.19547339556637475No Hit
GCTCATAGCAGAGCAGCGAGATCCTCAGTGCCAGGAGTGAGACGGGAAAT30330.1936853344504458No Hit
AAATTACACTGTTGGTTCGGTGGGAAAGAATTTGACCTAGACTCTGCCTT29790.19023693086972568No Hit
CTCAATTACTATGTGGTCACTCAATACTTCCATGTTGTCAGAGAGTACTT29540.18864044773050342No Hit
CAATAGGACATTTAAATCAAATAAAAAGAGGAGTAAACATGAAAATAAGA29250.1867885272890056No Hit
GTCATGTACCTGAATCCTGGAAATTATTCAATGCAAGTGAAACTAGGAAC29170.18627765268445448No Hit
CTGATATACAGAAAGCACTAATTGGTGCCTCTATCTGCTTTTTAAAACCC28470.1818074998946321No Hit
TGTTTGAACCATTCCAGATTCTTTCAATTTGTTCTTTTATTTTATCAGCT27880.17803979968606756No Hit
GTACTTCCTTCATTGCTTCTTTAGCCTGAATTTCTTTTTGGTAACTGTGT27800.1775289250815164No Hit
GTCTCATGGTCATGTACCTGAATCCTGGAAATTATTCAATGCAAGTGAAA27730.1770819098025342No Hit
GCACTAATTGGTGCCTCTATCTGCTTTTTAAAACCCAAAGACCAGGAAAG27250.17401666217522743No Hit
CTCCAATGTTGCTTTGCAGTTCTTCTGCCAGTTTTTGGACGTCTTCTCCT26650.170185102641094No Hit
GACAATAAACAGAGAGGTATCAATTTTGAGACACAGTTACCAAAAAGAAA26490.16916335343199174No Hit
GACCTAGACTCTGCCTTGGAATGGATAAAAAACAAAAGATGCTTAACTGA25730.16431004468875604No Hit
CTCAATACTTCCATGTTGTCAGAGAGTACTTCCTTCATTGCTTCTTTAGC25730.16431004468875604No Hit
GGACAATAGGACATTTAAATCAAATAAAAAGAGGAGTAAACATGAAAATA25610.16354373278192935No Hit
ACAATAAACAGAGAGGTATCAATTTTGAGACACAGTTACCAAAAAGAAAT25600.16347987345636047No Hit
GGATGTAATGGAAGTGCTAAAGCAGAGCTCTATGGGAAATTCAGCTCTTG25390.16213882761941376No Hit
GTTCTATAGAGATGGATTTGTATCTAACTTTGCAATGGAAATTCCTTCAT24980.15952059527108922No Hit
CTCCAAAACTGTTTCACCCATTTTTATTATCTCTTCAGCAGAAAGCCCCT24020.1533901000164757No Hit
GTATAGTATGGTTATCCAATCCATCATCATTTAAAGATGCAGCAGTAATG23560.15045257104030674No Hit
GCTTAAATGCATAGTAAGAAATACAGTAAAAATTGAATTTAATGCGATTC23240.14840907262210223No Hit
CAATAAACAGAGAGGTATCAATTTTGAGACACAGTTACCAAAAAGAAATT23050.1471957454362933No Hit
GCACAGAGCGTTCCTAGTTTCACTTGCATTGAATAATTTCCAGGATTCAG22470.14349190455329763No Hit
CCTTTATTCTTATTTTCATGTTTACTCCTCTTTTTATTTGATTTAAATGT22380.1429171706231776No Hit
GTTCAAACATTATAGGTATTTCTTCACAAGAGCTGAATTTCCCATAGAGC22070.140937531530542No Hit
TTCCAGATTCTTTCAATTTGTTCTTTTATTTTATCAGCTCTCCATTTCAT21830.13940490771688863No Hit
TCTTTAGCCTGAATTTCTTTTTGGTAACTGTGTCTCAAAATTGATACCTC21780.13908561108904416No Hit
CAATACTCCAATGTTGCTTTGCAGTTCTTCTGCCAGTTTTTGGACGTCTT21690.13851087715892413No Hit
ATTCATCACAGAGCCCTTATCGGGAATGGGAACAACAGCAACAAAAAAGA21490.13723369064754634No Hit
GATTACAATTTGCTTAAATGCATAGTAAGAAATACAGTAAAAATTGAATT21470.13710597199640856No Hit
CTATTGTCCAAGCCATGAAATGGAGAGCTGATAAAATAAAAGAACAAATT21450.13697825334527078No Hit
GAATAATTTCCAGGATTCAGGTACATGACCATGAGACAATATAGTAGCGC21310.1360842227873063No Hit
AATAAAAAGAGGAGTAAACATGAAAATAAGAATAAAGGGGCCAAATAAAG21060.13448773964808403No Hit
GGTTCAAACATTATAGGTATTTCTTCACAAGAGCTGAATTTCCCATAGAG21020.13423230234580846No Hit
GAAATACAGTAAAAATTGAATTTAATGCGATTCTTCTACCTCCAAAACTG20910.13352984976455065No Hit
ATTCCAGATTCTTTCAATTTGTTCTTTTATTTTATCAGCTCTCCATTTCA20910.13352984976455065No Hit
GTCCAAGCCATGAAATGGAGAGCTGATAAAATAAAAGAACAAATTGAAAG20650.13186950729975952No Hit
ACTATGTGGTCACTCAATACTTCCATGTTGTCAGAGAGTACTTCCTTCAT20600.13155021067191505No Hit
AGCAGGCACTTTCTTAAAATGTCGCTGTTTGGAGACACAATTGCCTACCT20600.13155021067191505No Hit
AGTCAGAAGAATGGGGAAGGAATTGCAAAGGATGTAATGGAAGTGCTAAA20350.1299537275326928No Hit
CCTTTAATGCAGGAGAATTTTCTCTCCCCACTTTTGATTCATTGAACATT19380.12375937295251037No Hit
CCATTACATCCTTTGCAATTCCTTCCCCATTCTTCTGACTTGCCCCAAGG19270.12305692037125257No Hit
GCTGTAGACATAGGAAACGGATGCTTCGAAACCAAACACAAATGCAACCA18740.11967237611610136No Hit
CTTCAGCAGAAAGCCCCTCAATTACTATGTGGTCACTCAATACTTCCATG18680.11928922016268802No Hit
TTATTATTGTCATTCCTATTGCCATATCTGCTGATTCATTTACTCCAGCA18170.11603239455867459No Hit
ATGCTTAACTGATATACAGAAAGCACTAATTGGTGCCTCTATCTGCTTTT18030.1151383640007101No Hit
TTCACACAGAGCTCATAGCAGAGCAGCGAGATCCTCAGTGCCAGGAGTGA18000.11494678602400345No Hit
TTTTTATTATCTCTTCAGCAGAAAGCCCCTCAATTACTATGTGGTCACTC17920.11443591141945232No Hit
GTTTAATATGCTATCTACCGTGTTGGGAGTAGCAGCACTAGGCATCAAAA17830.1138611774893323No Hit
TTCTTAAAATGTCGCTGTTTGGAGACACAATTGCCTACCTGCTTTCATTG17500.1117538197455589No Hit
CTTGTGAAGAAATACCTATAATGTTTGAACCATTCCAGATTCTTTCAATT17450.11143452311771446No Hit
TTGCAATTCCTTCCCCATTCTTCTGACTTGCCCCAAGGGATCTCAATACT17240.11009347728076775No Hit
CAATATAGTAGCGCTGAGCTTTCATGGCCTTCTGCTATTTCAAATGCTTC17180.1097103213273544No Hit
AATAAAGAGACAATAAACAGAGAGGTATCAATTTTGAGACACAGTTACCA17040.10881629076938994No Hit
AAATTATTCAATGCAAGTGAAACTAGGAACGCTCTGTGCTTTGTGCGAAA16920.10804997886256325No Hit
TAGTAGCGCTGAGCTTTCATGGCCTTCTGCTATTTCAAATGCTTCATGAA16760.10702822965346098No Hit
TTCATGGCCTTCTGCTATTTCAAATGCTTCATGAAAGCTCACACATTTTC16650.1063257770722032No Hit
GGCTTGGACAATAGGACATTTAAATCAAATAAAAAGAGGAGTAAACATGA16580.10587876179322096No Hit
ACAGAGAGGTATCAATTTTGAGACACAGTTACCAAAAAGAAATTCAGGCT16410.1047931532585498No Hit
CTACAATAAAGGAAAATACAAGGCCTAACCTTCCTTATAGACAGATGGAG16350.10440999730513646No Hit
GCGCTACTATATTGTCTCATGGTCATGTACCTGAATCCTGGAAATTATTC16280.10396298202615423No Hit
TCTCAATACTCCAATGTTGCTTTGCAGTTCTTCTGCCAGTTTTTGGACGT16220.10357982607274087No Hit
CATTTAAATCAAATAAAAAGAGGAGTAAACATGAAAATAAGAATAAAGGG16160.10319667011932754No Hit
ATTTAAATGTCCTATTGTCCAAGCCATGAAATGGAGAGCTGATAAAATAA16080.1026857955147764No Hit
GTCAGAGAGTACTTCCTTCATTGCTTCTTTAGCCTGAATTTCTTTTTGGT15950.10185562428238082No Hit
GCCCCAAGGGATCTCAATACTCCAATGTTGCTTTGCAGTTCTTCTGCCAG15940.10179176495681194No Hit
GTAAAAATTGAATTTAATGCGATTCTTCTACCTCCAAAACTGTTTCACCC15890.10147246832896749No Hit
TGCTAAAGCAGAGCTCTATGGGAAATTCAGCTCTTGTGAAGAAATACCTA15870.1013447496778297No Hit
CCTTTTCCCATCCCATTCATTGTTTTTGCTGTGTTCATAGCTGAGACCAT15830.10108931237555413No Hit
TTCTAATGGTATGCTGAACAGATCAGGACAAGGTATTTGAGCCTTTAGTC15800.10089773439884747No Hit
GATTCTGGCTGAGAGAAAAATGAGAAAATGTGTGAGCTTTCATGAAGCAT15790.10083387507327857No Hit
CTATGTGGTCACTCAATACTTCCATGTTGTCAGAGAGTACTTCCTTCATT15760.1006422970965719No Hit
ACATTGGAGTATTGAGATCCCTTGGGGCAAGTCAGAAGAATGGGGAAGGA15710.10032300046872746No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCACCC202.1249907E-6145.0821145
GAGCGGA100.0071090697144.878282
ATAAGCC650.0133.92195145
GGCACCC403.274181E-11126.946846145
GCCGACC301.0982876E-7120.901764145
TGACGCC850.0119.47939145
CACGCAC8200.0113.075733
GCACGCA8300.0111.713382
AGCACGC8350.0110.17691
GAGCAGA1550.0107.490341
AAACACG2900.0102.42055
GAAACAC3050.097.380324
CGAATCC259.366656E-487.04927145
ACACGTG1000.086.93537
AACACGT1000.086.93536
CGGTGCA259.419117E-486.926962
ACAACGC8400.085.3856058
CAACGCA8550.083.895659
CGATAAA801.8189894E-1281.60869145
GGTGCGT451.2386827E-680.495656
Produced by FastQC (version 0.11.5)