FastQCFastQC Report
Thu 11 Jul 2019
HWC5JBCX2_l01_n02_SM2_2012_P1_DM2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWC5JBCX2_l01_n02_SM2_2012_P1_DM2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1780044
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCCAACACCATGTCAAGCTTTCAGGTAGACTGTTTTCTTTGGCATATTC111340.6254901564230997No Hit
GTTGTAAGGCTTGCATAAATGTTATTTGTTCGAAACTATTCTCTGTCGCT102950.5783564900642906No Hit
CTTATGAGGATGTCAAAAATGCAGTTGGGGTCCTCATCGGAGGACTTGAG98350.552514432227518No Hit
CCTCATAAGTATGTCCTGGAAGAGAAGGTAATGGTGAAATTTCTCCAACT83640.4698760255364474No Hit
GGTTTATGCTTATGCCTAGGCAAAAGATAATAGGCCCTCTTTGCGTGCGA82490.4634155110772543No Hit
AAACAAGGGTGTTTTTTATCATTAAATAAGCTGAAACGAGAAAGCTCTTA75510.42420299722928195No Hit
CCCTTGGCCTCGACATCAAAACAGCCACTCTTGTTGGGAAACAAATTGTG64880.36448537227169664No Hit
ACTTCAAGCAGTAGTTGTAAGGCTTGCATAAATGTTATTTGTTCGAAACT62990.3538676572039792No Hit
GGATTGGGTGATGCCCCATTCCTTGATCGGCTACGCCGAGATCAAAAGTC62020.3484183537036163No Hit
GCATAAATGTTATTTGTTCGAAACTATTCTCTGTCGCTTTCAATCTGTGC54980.3088687695360339No Hit
ATATACAACAGGATGGGAACAGTGACCACAGAAGCTGCTTTTGGTCTAGT54060.3037003579686794No Hit
GTAGAAACAAGGGTGTTTTTTATCATTAAATAAGCTGAAACGAGAAAGCT53200.29886901672093497No Hit
GCATAAACCAGTCTCGTGACATTTCCTCGAGGGTCATGTCAGAAATGTAA49140.27606059176065306No Hit
ACTAAGGGCTTTCACTGAGGAGGGAGCAATAGTTGGAGAAATTTCACCAT47430.2664540876517659No Hit
CCTTATTTCCTCAAATTTCCGTCCCAATTGTTCCCGCCACTTCTCATTTC46700.26235306542984327No Hit
GTTCTTATCCATGACCGCCTGGTCCAATCGCACGCAAAGAGGGCCTATTA46550.2615103896308181No Hit
AGATAATAGGCCCTCTTTGCGTGCGATTGGACCAGGCGGTCATGGATAAG44430.24960057167126207No Hit
ACATAATGGAATCCAACACCATGTCAAGCTTTCAGGTAGACTGTTTTCTT43620.24505012235652604No Hit
CTACTAAGGGCTTTCACTGAGGAGGGAGCAATAGTTGGAGAAATTTCACC43450.24409508978429748No Hit
CTTTCAATCTGTGCCGTATTTCTTCAATTAACCACCTTATTTCCTCAAAT42630.23948846208295974No Hit
GTGTTACCATTCCACTCAAGTCCTCCGATGAGGACCCCAACTGCATTTTT41940.23561215340744385No Hit
GCTCTTATCTCTTGCTCTACTTCAAGCAGTAGTTGTAAGGCTTGCATAAA41820.2349380127682237No Hit
GCTCTATGTTGACAAAATGACCATCGTCAACATCCACAGCACTCTGCTGT40180.22572475736554826No Hit
TCTCATTTCTGCTCTGGAGGTAGTGAAGGTCTCCCATTCTCATCACAGCT38280.21505086391122916No Hit
GAAGAGGCAACACCCTTGGCCTCGACATCAAAACAGCCACTCTTGTTGGG38260.2149385071380258No Hit
GATAAGAACATAGTACTGGAAGCAAACTTCAGTATAATCTTCAACCGATT36920.20741060333340075No Hit
CCTTGATACTACTAAGGGCTTTCACTGAGGAGGGAGCAATAGTTGGAGAA36680.20606232205496045No Hit
GTCCCAATAGTTCTCATTGCATGTACCATCTGCCTAGTCTTATTAGCAAC36280.20381518659089323No Hit
GTTGTATATGAGGCCCATGCAACTGGCAAGTGCACCAGTTGAATAGCTTA35150.19746702890490347No Hit
GTATAATCTTCAACCGATTAGAGACCTTGATACTACTAAGGGCTTTCACT35130.1973546721317001No Hit
CTCTTATCTCTTGCTCTACTTCAAGCAGTAGTTGTAAGGCTTGCATAAAT34480.19370307700259096No Hit
AAACTATTCTCTGTCGCTTTCAATCTGTGCCGTATTTCTTCAATTAACCA33860.1902200170332868No Hit
GTACTATGTTCTTATCCATGACCGCCTGGTCCAATCGCACGCAAAGAGGG33720.1894335196208633No Hit
ATCTTCAACCGATTAGAGACCTTGATACTACTAAGGGCTTTCACTGAGGA33140.1861751731979659No Hit
GTGCCGTATTTCTTCAATTAACCACCTTATTTCCTCAAATTTCCGTCCCA33110.18600663803816087No Hit
ACTATGTTCTTATCCATGACCGCCTGGTCCAATCGCACGCAAAGAGGGCC33070.18578192449175415No Hit
CTCTTGCTCTACTTCAAGCAGTAGTTGTAAGGCTTGCATAAATGTTATTT32980.185276319012339No Hit
GTTTTTTATCATTAAATAAGCTGAAACGAGAAAGCTCTTATCTCTTGCTC32710.18375950257409368No Hit
CTATTGGGACTCATCCTAGCTCCAGTGCTGGTCTGAGAGATGACCTTCTT32520.18269211322866177No Hit
ATCCATGACCGCCTGGTCCAATCGCACGCAAAGAGGGCCTATTATCTTTT32160.1806696913110013No Hit
GGATAGAGCAGTTAAACTATACAAGAAGCTCAAAAGAGAAATAACGTTCC32000.17977083712537442No Hit
CTCTAGCTCTATGTTGACAAAATGACCATCGTCAACATCCACAGCACTCT31670.177916950367519No Hit
GTATCAAGGTCTCTAATCGGTTGAAGATTATACTGAAGTTTGCTTCCAGT31580.17741134488810387No Hit
CTCTTGTTGGGAAACAAATTGTGGAATGGATTTTGAAAGAGGAATCCAGC31010.17420917685180815No Hit
GTCTTATTAGCAACCTCCATGGCCTCTGCTGCCTGTTCACTCGATCCAGC30690.17241146848055441No Hit
CAATGGATTGGGTGATGCCCCATTCCTTGATCGGCTACGCCGAGATCAAA30450.1710631872021141No Hit
GGCATAAGCATAAACCAGTCTCGTGACATTTCCTCGAGGGTCATGTCAGA30000.16853515980503853No Hit
GTACTGGAAGCAAACTTCAGTATAATCTTCAACCGATTAGAGACCTTGAT29980.16842280303183516No Hit
GCTTATGCCTAGGCAAAAGATAATAGGCCCTCTTTGCGTGCGATTGGACC29740.16707452175339485No Hit
GCTCTACTTCAAGCAGTAGTTGTAAGGCTTGCATAAATGTTATTTGTTCG29330.164771207902726No Hit
GTAGTTGTAAGGCTTGCATAAATGTTATTTGTTCGAAACTATTCTCTGTC29290.16454649435631927No Hit
GCTCTGGAGGTAGTGAAGGTCTCCCATTCTCATCACAGCTTCTCCAAGCG28730.16140050470662523No Hit
GTGCTAGCCAGCACCATTCTGTTTTCATGCCTGATTAGTGGATTGGTGGT28480.15999604504158324No Hit
CTGCTGTTCCTGTTGATATTCTTCCCTCATGGACTCAGGCACTCCTTCCG28260.15876012053634628No Hit
GAACATAGTACTGGAAGCAAACTTCAGTATAATCTTCAACCGATTAGAGA28140.15808597989712614No Hit
CTCATGGAATGGCTAAAGACAAGACCAATCTTGTCACCTCTGACTAAGGG28090.15780508796411774No Hit
TTTCTGACATGACCCTCGAGGAAATGTCACGAGACTGGTTTATGCTTATG27760.15595120120626232No Hit
TGGTAACACGGTTCGAGTCTCTGAAAATATACAGAGATTCGCTTGGAGAA27320.15347935219578843No Hit
ATTTAAAGATGAGTCTTCTAACCGAGGTCGAAACGTACGTTCTTTCTATC27270.15319846026278003No Hit
GTTTTCTTTGGCATATTCGCAAGCGATTTGCAGACAATGGATTGGGTGAT26660.14977157868007757No Hit
CATCTGTACCTACTTCGCGTTACATTTCTGACATGACCCTCGAGGAAATG26600.14943450836046748No Hit
GTACCTACTTCGCGTTACATTTCTGACATGACCCTCGAGGAAATGTCACG26420.14842329740163726No Hit
CTTTTCAAACCGTATTTAAAGCGACGATAAATGCATTTGAAAAAAAGACG26240.14741208644280704No Hit
GTTTTTTACTCTAGCTCTATGTTGACAAAATGACCATCGTCAACATCCAC26150.1469064809633919No Hit
ACATAGTACTGGAAGCAAACTTCAGTATAATCTTCAACCGATTAGAGACC25880.14538966452514657No Hit
GCCTAGTCTTATTAGCAACCTCCATGGCCTCTGCTGCCTGTTCACTCGAT25810.1449964158189348No Hit
GTCATGGATAAGAACATAGTACTGGAAGCAAACTTCAGTATAATCTTCAA25700.14437845356631634No Hit
ATTCTCTGTCGCTTTCAATCTGTGCCGTATTTCTTCAATTAACCACCTTA25460.143030172287876No Hit
GTTGAAGATTATACTGAAGTTTGCTTCCAGTACTATGTTCTTATCCATGA25140.1412324639166223No Hit
CTCATGGACTCAGGCACTCCTTCCGTAGAAGGCCCTCTTTTCAAACCGTA25100.14100775037021557No Hit
GTAATCCACAATATCAGGTGCAAGATCCCAATGATGTTTGCTGCAATGAC25050.14072685843720717No Hit
ATACTGAAGTTTGCTTCCAGTACTATGTTCTTATCCATGACCGCCTGGTC24850.13960329070517358No Hit
TCCTAGCTCCAGTGCTGGTCTGAGAGATGACCTTCTTGAAAATTTGCAGG24540.1378617607205215No Hit
GTATATTTTCAGAGACTCGAACCGTGTTACCATTCCACTCAAGTCCTCCG24470.13746851201430976No Hit
CTGTATATTTTCAGAGACTCGAACCGTGTTACCATTCCACTCAAGTCCTC24420.13718762008130136No Hit
CCCTTAGTAGTATCAAGGTCTCTAATCGGTTGAAGATTATACTGAAGTTT24210.1360078739626661No Hit
AGCGAAAGCAGGGTGACAAAAACATAATGGAATCCAACACCATGTCAAGC24050.1351090197770392No Hit
CTTCAGTATAATCTTCAACCGATTAGAGACCTTGATACTACTAAGGGCTT23610.13263717076656534No Hit
CTTATCTCTTGCTCTACTTCAAGCAGTAGTTGTAAGGCTTGCATAAATGT23450.13173831658093846No Hit
TCACTGAGGAGGGAGCAATAGTTGGAGAAATTTCACCATTACCTTCTCTT23360.13123271110152332No Hit
AAAGCAGGGTGACAAAAACATAATGGAATCCAACACCATGTCAAGCTTTC23360.13123271110152332No Hit
CTCCCATTCTCATCACAGCTTCTCCAAGCGAATCTCTGTATATTTTCAGA23090.129715894663278No Hit
CATCACAGCTTCTCCAAGCGAATCTCTGTATATTTTCAGAGACTCGAACC22970.12904175402405782No Hit
GTATTTCTTCAATTAACCACCTTATTTCCTCAAATTTCCGTCCCAATTGT22960.12898557563745616No Hit
TCTCTGTATATTTTCAGAGACTCGAACCGTGTTACCATTCCACTCAAGTC22870.12847997015804105No Hit
ATTAGAGACCTTGATACTACTAAGGGCTTTCACTGAGGAGGGAGCAATAG22850.12836761338483768No Hit
GTTCCATAGCCTTTGCCGTAGTGCTAGCCAGCACCATTCTGTTTTCATGC22820.12819907822503263No Hit
GTCTTCTAACCGAGGTCGAAACGTACGTTCTTTCTATCATCCCGTCAGGC22560.12673844017338898No Hit
ATACAAGAAGCTCAAAAGAGAAATAACGTTCCATGGGGCCAAGGAGGTGT22170.12454748309592346No Hit
TGATACTACTAAGGGCTTTCACTGAGGAGGGAGCAATAGTTGGAGAAATT21890.12297448827107643No Hit
CACTTAGAATGACAATTGCATCTGTACCTACTTCGCGTTACATTTCTGAC21590.12128913667302606No Hit
GGCTAGCACTACGGCAAAGGCTATGGAACAGGTGGCTGGATCGAGTGAAC21430.12039028248739918No Hit
CTTGTGAACAGATTGCTGATTCACAGCATCGGTCTCACAGACAAATGGCT21260.11943524991517064No Hit
CTTCCAGGACATACTTATGAGGATGTCAAAAATGCAGTTGGGGTCCTCAT21140.11876110927595049No Hit
CTATTGCTCCCTCCTCAGTGAAAGCCCTTAGTAGTATCAAGGTCTCTAAT21110.11859257411614545No Hit
ATGTCAAGCTTTCAGGTAGACTGTTTTCTTTGGCATATTCGCAAGCGATT21000.11797461186352698No Hit
TCCATAGCCTTTGCCGTAGTGCTAGCCAGCACCATTCTGTTTTCATGCCT20830.11701957929129841No Hit
GCATGTACCATCTGCCTAGTCTTATTAGCAACCTCCATGGCCTCTGCTGC20780.11673868735829002No Hit
CCACAGCACTCTGCTGTTCCTGTTGATATTCTTCCCTCATGGACTCAGGC20780.11673868735829002No Hit
CCTTAAAAGGAAGAGGCAACACCCTTGGCCTCGACATCAAAACAGCCACT20740.11651397381188329No Hit
GTCCAATCGCACGCAAAGAGGGCCTATTATCTTTTGCCTAGGCATAAGCA20590.1156712980128581No Hit
GTACAGATGCAATTGTCATTCTAAGTGTCTCGCTGGATTCCTCTTTCAAA20570.11555894123965475No Hit
CAGTAGTTGTAAGGCTTGCATAAATGTTATTTGTTCGAAACTATTCTCTG20290.11398594641480772No Hit
GATGAGTCTTCTAACCGAGGTCGAAACGTACGTTCTTTCTATCATCCCGT20220.11359269770859597No Hit
GTAGTATCAAGGTCTCTAATCGGTTGAAGATTATACTGAAGTTTGCTTCC20180.11336798416218925No Hit
CATCAAAACAGCCACTCTTGTTGGGAAACAAATTGTGGAATGGATTTTGA20130.11308709222918084No Hit
TCATTAAATAAGCTGAAACGAGAAAGCTCTTATCTCTTGCTCTACTTCAA19990.11230059481675733No Hit
GCACTGGAGCTAGGATGAGTCCCAATAGTTCTCATTGCATGTACCATCTG19650.11039052967230023No Hit
AGCCAGCACCATTCTGTTTTCATGCCTGATTAGTGGATTGGTGGTAGTAG19640.11033435128569855No Hit
CTATACAAGAAGCTCAAAAGAGAAATAACGTTCCATGGGGCCAAGGAGGT19360.10876135646085154No Hit
AGTATGTCCTGGAAGAGAAGGTAATGGTGAAATTTCTCCAACTATTGCTC19360.10876135646085154No Hit
GTCATACTCCTCTGCATTGTCTCCGAAGAAATAAGACCCTTCATTACTCA19310.10848046452784313No Hit
CTTCTAACCGAGGTCGAAACGTACGTTCTTTCTATCATCCCGTCAGGCCC19280.1083119293680381No Hit
GATCAAAAGTCCTTAAAAGGAAGAGGCAACACCCTTGGCCTCGACATCAA19270.10825575098143642No Hit
CAGGTAGACTGTTTTCTTTGGCATATTCGCAAGCGATTTGCAGACAATGG19170.1076939671154196No Hit
TCACTAAGCTATTCAACTGGTGCACTTGCCAGTTGCATGGGCCTCATATA19140.10752543195561458No Hit
GATAATAGGCCCTCTTTGCGTGCGATTGGACCAGGCGGTCATGGATAAGA19070.10713218324940282No Hit
CTATCATCCCGTCAGGCCCCCTCAAAGCCGAGATCGCACAGAGACTGGAA19010.10679511292979275No Hit
GACTCGAACCGTGTTACCATTCCACTCAAGTCCTCCGATGAGGACCCCAA19010.10679511292979275No Hit
CCTCTGCATTGTCTCCGAAGAAATAAGACCCTTCATTACTCATGTCAAAG19010.10679511292979275No Hit
GTTCGAAACTATTCTCTGTCGCTTTCAATCTGTGCCGTATTTCTTCAATT18950.10645804261018267No Hit
GCTCTATCCATGTTGTTCGGGTCCCCATTTCCATTTAGGGCATTTTGGAT18700.1050535829451407No Hit
GGACATACTTATGAGGATGTCAAAAATGCAGTTGGGGTCCTCATCGGAGG18680.10494122617193732No Hit
GGTAACACGGTTCGAGTCTCTGAAAATATACAGAGATTCGCTTGGAGAAG18580.10437944230592053No Hit
CCACAATATCAGGTGCAAGATCCCAATGATGTTTGCTGCAATGACGAGAG18570.10432326391931884No Hit
GTCTTATACAATCCAGCCCTGTTAGTTCTGGATGCTGAACAAAACTCCCG18510.10398619359970877No Hit
TTCCAGTACTATGTTCTTATCCATGACCGCCTGGTCCAATCGCACGCAAA18410.10342440973369196No Hit
GTGCTGGCTAGCACTACGGCAAAGGCTATGGAACAGGTGGCTGGATCGAG18340.10303116102748022No Hit
GTTCACAGCAGTGGGTAAAGAGTTCAACCACCTGGAAAAAAGAATAGAGA18340.10303116102748022No Hit
GTTCACAAGTGGCACAAACTAGACCAAAAGCAGCTTCTGTGGTCACTGTT18330.10297498264087854No Hit
GAGCAATAGTTGGAGAAATTTCACCATTACCTTCTCTTCCAGGACATACT18230.10241319877486174No Hit
TTCTAAGTGTCTCGCTGGATTCCTCTTTCAAAATCCATTCCACAATTTGT18180.10213230684185334No Hit
CCTCAGTGAAAGCCCTTAGTAGTATCAAGGTCTCTAATCGGTTGAAGATT18160.10201995006864999No Hit
GTATTTAAAGCGACGATAAATGCATTTGAAAAAAAGACGATCAGTAATCC17940.10078402556341305No Hit
CTCCAGTGCTGGTCTGAGAGATGACCTTCTTGAAAATTTGCAGGCCTACC17930.10072784717681137No Hit
GGCTAAAGACAAGACCAATCTTGTCACCTCTGACTAAGGGAATTTTAGGA17910.100615490403608No Hit
CCCATTCGCTTCTGGTAGGCCTGCAAATTTTCAAGAAGGTCATCTCTCAG17830.10016606331079456No Hit
TCCCAATTGTTCCCGCCACTTCTCATTTCTGCTCTGGAGGTAGTGAAGGT17810.1000537065375912No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGGGGG100.007076644145.10052145
CGTTTAT100.0071124225144.855972
GAGCGAA800.0126.748981
AATCGCC301.09752364E-7120.917114145
CCTCGCC850.0119.49456145
CGCGAAA800.0117.89418145
AGCGTAA256.4440246E-6116.08044145
TCACGCC1250.0116.08043145
AGTAGAA6100.097.362221
AGACGAA554.1472958E-1092.3367145
AGGCTCC554.1472958E-1092.3367145
GCGAAAG5350.090.7042162
CGAAAGC5300.090.193353
ACCGCCC259.362143E-487.060326145
ATTGTCC1000.087.060326145
CGATTAA502.258821E-887.060326145
GCGATCC259.362143E-487.060326145
GGTTAAA1450.085.05893145
GTAGAAA14950.082.8440551
TGACGCC554.375397E-879.145744145
Produced by FastQC (version 0.11.5)