FastQCFastQC Report
Thu 11 Jul 2019
HWC5JBCX2_l01_n02_SM2_164_P1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWC5JBCX2_l01_n02_SM2_164_P1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences302476
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGCACGCACTTTCTTAAAATGTCGCTGTTTGGAGACACAATTGCCTACTT10280.33986167497586584No Hit
GTAGTAACAAGAGCATTTTTCAATAACGTTTCTTTGTAATGACAACAAGC6560.21687671087954086No Hit
CTACTATACTGTCTCATGGTCATGTACCTGAATCCTGGAAATTATTCAAT5720.1891059125352094No Hit
ATTATTATGTGGTCATTCAATACCTCCATGTTGTCAGAGAGCACTTCCTT5150.17026144223012735No Hit
GTATAGTAGCGCTGAGCTTTCATGGCCTTCTGCTATTTCAAATGCTTCAT4870.16100450944868352No Hit
CTGTAGAACATGCTTGTAAATTCAAACATTCCAGTCTCATTACAGTAACT4610.15240878615162856No Hit
GTGTAATACCACTGACAACAACACCCACCAAATCTCATTTTGCAAATCTC4360.144143667596768No Hit
GTATAGTATGATTATCCAATCCATCGTCATTTAAAGATGCAGCAGTAATA4350.14381306285457357No Hit
TCATAGATGTGCCCGTACAGGCAGCAATTTCAACAACATTCCCATACACT3910.12926645419801902No Hit
GTAAGAAATACAGTAAAATTGAATTTAATGCAATTCTTCTATCTCCAAAA3850.1272828257448525No Hit
GTTGTATTCAGTGCCGTTGCAGCAGGTTGGTTTCTGCATACTGTCCAATC3740.12364617358071384No Hit
GAATACAACAATAACCTCTTTTAGGTTGAATGATTTAAATGGAGCCGACA3610.11934831193218635No Hit
GTTTAATATGCTATCTACCGTGTTGGGAGTAGCTGCACTAGGTATCAAGA3550.11736468347901984No Hit
GTTTAGTATACCATTAGAAAGATATAATGAAGAAACAAGGGCAAAATTGA3470.11471984554146444No Hit
GTAGTAACAAGAGCCTTTTTTCATTTTAATCATTTGTGTATCGCATGTAT3420.11306682183049234No Hit
TCTATATGCTTTTTAAAACCCAAAGACCAGGAAAGAAAAAGAAGATTCAT3380.11174440286171464No Hit
CCATTAGAAAGATATAATGAAGAAACAAGGGCAAAATTGAAGAAGCTAAA3300.10909956492415927No Hit
GTAGTAACAAGAGCATTTTTCAGAAACAATTAAGTCTAGTAAGGACAATT3280.10843835543977043No Hit
GTATTTACACAGTCTCTCCAAAGCTTCCATAAGAAATTTTCGTTAGCTTT3270.10810775069757601No Hit
GCCTTGGAATGGATAAAAAACAAAAGATGCTTAACTGATATACAAAAAGC3170.10480170327563179No Hit
GTTCATAGCTGAGACCATCTGCATTTCTCGTCTCACTCCAGGCACTGAAG3160.10447109853343736No Hit
GTTCTACAGAGATGGATTTGTATCTAATTTTGCAATGGAACTCCCTTCGT3160.10447109853343736No Hit
GTAATATTCAGTGAATCAAAGGTGGGGAGAGAAAATTCTCCTGCATCAAA3130.1034792843068541No Hit
GTATAGCACCGGTCCTGTTCTCCAATAAGATAGCAAGATTGGGGAAAGGA3120.10314867956465969No Hit
GAATATTACTGCTGCATCTTTAAATGACGATGGATTGGATAATCATACTA3060.10116505111149314No Hit
GCTTTAGCACTTCCATTACATCCTTTGCAATCCCTTCCCCATTCTTTTGA3040.1005038416271043No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACACCAC100.0071071982144.871838
AGCACGC1350.0128.732391
GCACGCA1400.0124.1347962
GTAACAA2600.0116.973184
CACGCAC1500.0115.859153
ACGCACT1450.0114.860364
CGCACTT1450.0114.860365
TAGAAAC1000.0108.617952
GAAACAC1100.092.16074
AAACACG1100.092.16075
AACACGA900.088.503526
GAAGCGG353.454146E-582.756531
CGTGCCT353.454146E-582.756533
ACGTGCC353.454146E-582.756532
ACGAGCC406.6848996E-572.435919
TAACAAG4150.071.5395365
TAGTAAC4550.066.841822
CACGAGC1200.066.39968
ACACGAG1200.066.388617
GTGTAAA902.8376235E-1064.3661961
Produced by FastQC (version 0.11.5)