FastQCFastQC Report
Tue 2 Jul 2019
HWC5JBCX2_l01_n02_SM2_1230_P1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWC5JBCX2_l01_n02_SM2_1230_P1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1433034
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCTAATCCTGTTCGCTCCCCACGCTTTCGAGCCTCAGCGTCAATGTTAG207501.4479768100407946No Hit
GTTTAGTCTAATGTGAAAGCCCACGGCTTAACCGTGGAACGGCATTGGAA192881.3459555041959925No Hit
GCTTAATACGTTAGCTCCAGCACCGAGGGCCGGAAAGCCCCCAACACTTA163211.1389122658638944No Hit
GTAATACGTAGGTGACAAGCGTTGTCCGGATTTATTGGGCGTAAAGGGAG131590.9182615346181597No Hit
GTAGTAACAAGAGCTGGTAAGGTTCTTCGCGTTGCTTCGAATTAAACCAC130840.9130278834975305No Hit
CTACTAGCGCCTTGACGGTATCTAACCAGAAAGTCACGGCTAACTACGTG128400.8960010718517495No Hit
GGTAAGGTTCTTCGCGTTGCTTCGAATTAAACCACATGCTCCACCGCTTG100130.6987273156114928No Hit
GTATCTAACCAGAAAGTCACGGCTAACTACGTGCCAGCAGCCGCGGTAAT88990.6209901509664112No Hit
AGGTAACTACTAGCGCCTTGACGGTATCTAACCAGAAAGTCACGGCTAAC88130.6149888976814227No Hit
GTAGAAACACGAGCTAGGTAACTACTAGCGCCTTGACGGTATCTAACCAG84600.5903558464069938No Hit
CACTTAGCACTCATCGTTTACGGCGTGGACTACCAGGGTATCTAATCCTG84560.5900767183472269No Hit
AACAAGAGCTGGTAAGGTTCTTCGCGTTGCTTCGAATTAAACCACATGCT77340.5396941035593015No Hit
CTCCAGCACCGAGGGCCGGAAAGCCCCCAACACTTAGCACTCATCGTTTA76440.5334137222145462No Hit
GATTTATTGGGCGTAAAGGGAGCGCAGGCGGTCTGTTTAGTCTAATGTGA72540.5061987363872734No Hit
GTCTAATGTGAAAGCCCACGGCTTAACCGTGGAACGGCATTGGAAACTGA67610.471796203021003No Hit
ACTTAGCACTCATCGTTTACGGCGTGGACTACCAGGGTATCTAATCCTGT65410.4564441597338235No Hit
GGATTAGATACCCTGGTAGTCCACGCCGTAAACGATGAGTGCTAAGTGTT63160.44074320637193537No Hit
CTCCACCGCTTGTGCGGGTCCCCGTCAATTCTTTTGAGTTTCAACCTTGC60070.4191805637549423No Hit
GCATTGGAAACTGACAGACTTGAATGTAGAAGAGGAAAATGGAATTCCAA54530.38052132747722667No Hit
CTACCAGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGAGCCTCAGCG54130.37773004687955763No Hit
CGGTAATACGTAGGTGACAAGCGTTGTCCGGATTTATTGGGCGTAAAGGG53200.3712403194899772No Hit
CGGCTTAACCGTGGAACGGCATTGGAAACTGACAGACTTGAATGTAGAAG52970.3696353331463175No Hit
GGTAACTACTAGCGCCTTGACGGTATCTAACCAGAAAGTCACGGCTAACT51410.35874933881540844No Hit
GTAGAAACACGTGCTAGGTAACTACTAGCGCCTTGACGGTATCTAACCAG50710.3538645977694877No Hit
GTGAAAGCCCACGGCTTAACCGTGGAACGGCATTGGAAACTGACAGACTT49850.34786334448449935No Hit
GAATTAAACCACATGCTCCACCGCTTGTGCGGGTCCCCGTCAATTCTTTT47660.33258108321226154No Hit
GCTTAACCGTGGAACGGCATTGGAAACTGACAGACTTGAATGTAGAAGAG47650.3325113011973198No Hit
GTTCGCTCCCCACGCTTTCGAGCCTCAGCGTCAATGTTAGACCAGAAAAT45320.31625209171589785No Hit
TATCTACGCATTCCACCGCTACACTTGGAATTCCATTTTCCTCTTCTACA44930.3135305931331706No Hit
GCGTAAAGGGAGCGCAGGCGGTCTGTTTAGTCTAATGTGAAAGCCCACGG42970.2998533182045925No Hit
GCGTAGATATTTGGAGGAACACCAGTGGCGAAGGCGATTTTCTGGTCTAA42710.29803898581610766No Hit
CTGGTAAGGTTCTTCGCGTTGCTTCGAATTAAACCACATGCTCCACCGCT42160.2942009749943128No Hit
ATGTTAGACCAGAAAATCGCCTTCGCCACTGGTGTTCCTCCAAATATCTA41400.28889754185874167No Hit
GTAACTACTAGCGCCTTGACGGTATCTAACCAGAAAGTCACGGCTAACTA40950.28575735118636403No Hit
CCTTGACGGTATCTAACCAGAAAGTCACGGCTAACTACGTGCCAGCAGCC39710.2771043813335901No Hit
GGTGACAAGCGTTGTCCGGATTTATTGGGCGTAAAGGGAGCGCAGGCGGT39170.273336152526737No Hit
CTACGTGCCAGCAGCCGCGGTAATACGTAGGTGACAAGCGTTGTCCGGAT37790.2637062344647789No Hit
GGCGTAAAGGGAGCGCAGGCGGTCTGTTTAGTCTAATGTGAAAGCCCACG37460.26140342797170196No Hit
CCCCCAACACTTAGCACTCATCGTTTACGGCGTGGACTACCAGGGTATCT37110.25896105744874165No Hit
CTGTAGAACATGCTTGTAAATTCAAACATTCCAGTCTCATTACAGTAACT36560.25512304662694674No Hit
GTCGTAATCCCCAGGCGGAGTGCTTAATACGTTAGCTCCAGCACCGAGGG36300.2533087142384619No Hit
CACGTGCTAGGTAACTACTAGCGCCTTGACGGTATCTAACCAGAAAGTCA36150.252261984014336No Hit
ATTATTATGTGGTCATTCAATACCTCCATGTTGTCAGAGAGCACTTCCTT35480.24758658901324043No Hit
GTGCTTAATACGTTAGCTCCAGCACCGAGGGCCGGAAAGCCCCCAACACT34770.242632065952378No Hit
GGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGAGCCTCAGCGTCAATG34380.2399105673696507No Hit
ATGCTCCACCGCTTGTGCGGGTCCCCGTCAATTCTTTTGAGTTTCAACCT33740.2354445184133803No Hit
GACTACCAGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGAGCCTCAG33710.2352351723685551No Hit
CTTGAATGTAGAAGAGGAAAATGGAATTCCAAGTGTAGCGGTGGAATGCG32820.22902457303874157No Hit
ATACGTAGGTGACAAGCGTTGTCCGGATTTATTGGGCGTAAAGGGAGCGC32800.22888500900885814No Hit
GAGCTGGTAAGGTTCTTCGCGTTGCTTCGAATTAAACCACATGCTCCACC32470.22658220251578118No Hit
GCTTCGAATTAAACCACATGCTCCACCGCTTGTGCGGGTCCCCGTCAATT32240.22497721617212152No Hit
GCTAACTACGTGCCAGCAGCCGCGGTAATACGTAGGTGACAAGCGTTGTC31500.21981334706643388No Hit
GACTTGAATGTAGAAGAGGAAAATGGAATTCCAAGTGTAGCGGTGGAATG31490.21974356505149217No Hit
GGTTCTTCGCGTTGCTTCGAATTAAACCACATGCTCCACCGCTTGTGCGG30950.21597533624463902No Hit
GCGTCAATGTTAGACCAGAAAATCGCCTTCGCCACTGGTGTTCCTCCAAA30600.21353296572167862No Hit
CACGAGCTAGGTAACTACTAGCGCCTTGACGGTATCTAACCAGAAAGTCA30270.21123015922860172No Hit
GTCCGGATTTATTGGGCGTAAAGGGAGCGCAGGCGGTCTGTTTAGTCTAA29590.20648498221256437No Hit
GACGGTATCTAACCAGAAAGTCACGGCTAACTACGTGCCAGCAGCCGCGG29560.20627563616773922No Hit
GTGTAGCGGTGGAATGCGTAGATATTTGGAGGAACACCAGTGGCGAAGGC28770.20076285698734292No Hit
TAACAAGAGCTGGTAAGGTTCTTCGCGTTGCTTCGAATTAAACCACATGC28030.1955989878816553No Hit
AGTCTAATGTGAAAGCCCACGGCTTAACCGTGGAACGGCATTGGAAACTG27630.19280770728398627No Hit
CTTTCGAGCCTCAGCGTCAATGTTAGACCAGAAAATCGCCTTCGCCACTG27010.18848122235759932No Hit
GTAAGAAATACAGTAAAATTGAATTTAATGCAATTCTTCTATCTCCAAAA26950.188062530267949No Hit
GTAACAAGAGCTGGTAAGGTTCTTCGCGTTGCTTCGAATTAAACCACATG26540.18520146765533824No Hit
GAATACAACAATAACCTCTTTTAGGTTGAATGATTTAAATGGAGCCGACA26140.18241018705766926No Hit
CGTTAGCTCCAGCACCGAGGGCCGGAAAGCCCCCAACACTTAGCACTCAT24710.17243135892100256No Hit
TCGTTTACGGCGTGGACTACCAGGGTATCTAATCCTGTTCGCTCCCCACG24620.17180332078652705No Hit
CATCGTTTACGGCGTGGACTACCAGGGTATCTAATCCTGTTCGCTCCCCA24610.17173353877158534No Hit
ATCCTGTTCGCTCCCCACGCTTTCGAGCCTCAGCGTCAATGTTAGACCAG24080.1680350919796739No Hit
ATGTAGAAGAGGAAAATGGAATTCCAAGTGTAGCGGTGGAATGCGTAGAT24080.1680350919796739No Hit
GTTCTTCGCGTTGCTTCGAATTAAACCACATGCTCCACCGCTTGTGCGGG23840.16636032362107248No Hit
GATATTTGGAGGAACACCAGTGGCGAAGGCGATTTTCTGGTCTAACATTG23720.1655229394417718No Hit
GCCCACGGCTTAACCGTGGAACGGCATTGGAAACTGACAGACTTGAATGT23480.16384817108317037No Hit
CCATTAGAAAGATATAATGAAGAAACAAGGGCAAAATTGAAGAAGCTAAA23200.16189427466480208No Hit
GTTTAATATGCTATCTACCGTGTTGGGAGTAGCTGCACTAGGTATCAAGA22670.15819582787289066No Hit
ATTTGGAGGAACACCAGTGGCGAAGGCGATTTTCTGGTCTAACATTGACG22420.15645127749934754No Hit
CCCCAGGCGGAGTGCTTAATACGTTAGCTCCAGCACCGAGGGCCGGAAAG22390.15624193145452236No Hit
GTAAGGTTCTTCGCGTTGCTTCGAATTAAACCACATGCTCCACCGCTTGT22010.1535902148867368No Hit
CGGCTAACTACGTGCCAGCAGCCGCGGTAATACGTAGGTGACAAGCGTTG21940.15310174078214472No Hit
GTGCTAAGTGTTGGGGGCTTTCCGGCCCTCGGTGCTGGAGCTAACGTATT21790.15205501055801884No Hit
CCCCAACACTTAGCACTCATCGTTTACGGCGTGGACTACCAGGGTATCTA21770.1519154465281354No Hit
TTCCACCGCTACACTTGGAATTCCATTTTCCTCTTCTACATTCAAGTCTG21700.15142697242354333No Hit
CCCTGGTAGTCCACGCCGTAAACGATGAGTGCTAAGTGTTGGGGGCTTTC21490.1499615501097671No Hit
CCGTGGAACGGCATTGGAAACTGACAGACTTGAATGTAGAAGAGGAAAAT21410.1494032939902333No Hit
GTTTAGTATACCATTAGAAAGATATAATGAAGAAACAAGGGCAAAATTGA21250.1482867817511657No Hit
AGCTAGGTAACTACTAGCGCCTTGACGGTATCTAACCAGAAAGTCACGGC21200.14793787167645708No Hit
ACTAGCGCCTTGACGGTATCTAACCAGAAAGTCACGGCTAACTACGTGCC21160.14765874361669018No Hit
TCCCCAGGCGGAGTGCTTAATACGTTAGCTCCAGCACCGAGGGCCGGAAA21150.14758896160174845No Hit
GCGTTGTCCGGATTTATTGGGCGTAAAGGGAGCGCAGGCGGTCTGTTTAG21000.14654223137762257No Hit
CATTGACGCTGAGGCTCGAAAGCGTGGGGAGCGAACAGGATTAGATACCC20770.1449372450339629No Hit
GTGCGGGTCCCCGTCAATTCTTTTGAGTTTCAACCTTGCGGTCGTAATCC20460.14277400257076941No Hit
CCACATGCTCCACCGCTTGTGCGGGTCCCCGTCAATTCTTTTGAGTTTCA20030.13977337592827524No Hit
GCTCCACCGCTTGTGCGGGTCCCCGTCAATTCTTTTGAGTTTCAACCTTG19940.13914533779379973No Hit
GGAAAATGGAATTCCAAGTGTAGCGGTGGAATGCGTAGATATTTGGAGGA19820.13830795361449905No Hit
AGTAACAAGAGCTGGTAAGGTTCTTCGCGTTGCTTCGAATTAAACCACAT19390.13530732697200487No Hit
TATCTAACCAGAAAGTCACGGCTAACTACGTGCCAGCAGCCGCGGTAATA19250.1343303787628207No Hit
CCAAATATCTACGCATTCCACCGCTACACTTGGAATTCCATTTTCCTCTT19250.1343303787628207No Hit
CACCAGTGGCGAAGGCGATTTTCTGGTCTAACATTGACGCTGAGGCTCGA19050.1329347384639862No Hit
TTCCAATGCCGTTCCACGGTTAAGCCGTGGGCTTTCACATTAGACTAAAC18960.1323067003295107No Hit
GAATGTAGAAGAGGAAAATGGAATTCCAAGTGTAGCGGTGGAATGCGTAG18950.13223691831456894No Hit
GTCTAACATTGACGCTGAGGCTCGAAAGCGTGGGGAGCGAACAGGATTAG18850.13153909816515172No Hit
CCTCCAAATATCTACGCATTCCACCGCTACACTTGGAATTCCATTTTCCT18650.1301434578663172No Hit
GACAAGCGTTGTCCGGATTTATTGGGCGTAAAGGGAGCGCAGGCGGTCTG18620.129934111821492No Hit
CCCCACGCTTTCGAGCCTCAGCGTCAATGTTAGACCAGAAAATCGCCTTC18530.1293060736870165No Hit
GGTCTGTTTAGTCTAATGTGAAAGCCCACGGCTTAACCGTGGAACGGCAT18400.12839890749277408No Hit
ATTAGTAGTAACAAGAGCTGGTAAGGTTCTTCGCGTTGCTTCGAATTAAA18350.12804999741806544No Hit
TATCTATAATGCTCGAACCATTTCAGATTCTTACAATTTGTTCTTTTATC18230.12721261323876473No Hit
ACGTAGGTGACAAGCGTTGTCCGGATTTATTGGGCGTAAAGGGAGCGCAG18110.12637522905946405No Hit
GTATCTAATCCTGTTCGCTCCCCACGCTTTCGAGCCTCAGCGTCAATGTT17860.12463067868592093No Hit
CCAGTGGCGAAGGCGATTTTCTGGTCTAACATTGACGCTGAGGCTCGAAA17830.12442133264109574No Hit
AATTAAACCACATGCTCCACCGCTTGTGCGGGTCCCCGTCAATTCTTTTG17670.12330482040202814No Hit
CGTAAAGGGAGCGCAGGCGGTCTGTTTAGTCTAATGTGAAAGCCCACGGC17570.1226070002526109No Hit
TTATTATTGTCATTCCTATTGCCATATCTGCTGATTCATTTACTCCAGCA17530.122327872192844No Hit
GTCACGGCTAACTACGTGCCAGCAGCCGCGGTAATACGTAGGTGACAAGC17450.12176961607331019No Hit
GTTCTACAGAGATGGATTTGTATCTAATTTTGCAATGGAACTCCCTTCGT17330.12093223189400949No Hit
ACCACATGCTCCACCGCTTGTGCGGGTCCCCGTCAATTCTTTTGAGTTTC17030.11883877144575775No Hit
GCGGTGGAATGCGTAGATATTTGGAGGAACACCAGTGGCGAAGGCGATTT17000.11862942540093256No Hit
GTGCTAGGTAACTACTAGCGCCTTGACGGTATCTAACCAGAAAGTCACGG16610.11590792681820529No Hit
CAATTATTATGTGGTCATTCAATACCTCCATGTTGTCAGAGAGCACTTCC16570.11562879875843839No Hit
GTTTCAACCTTGCGGTCGTAATCCCCAGGCGGAGTGCTTAATACGTTAGC16410.11451228651937079No Hit
ACACTTAGCACTCATCGTTTACGGCGTGGACTACCAGGGTATCTAATCCT16390.11437272248948734No Hit
AACACGAGCTAGGTAACTACTAGCGCCTTGACGGTATCTAACCAGAAAGT16260.11346555629524492No Hit
AGATTATACTTCAATACTATTTCTGGGATATGCAAGTTTCTCAAATTGTA16260.11346555629524492No Hit
AACCAGAAAGTCACGGCTAACTACGTGCCAGCAGCCGCGGTAATACGTAG16250.11339577428030319No Hit
TATTAGTAGTAACAAGAGCTGGTAAGGTTCTTCGCGTTGCTTCGAATTAA16220.11318642823547802No Hit
GAGTTTCAACCTTGCGGTCGTAATCCCCAGGCGGAGTGCTTAATACGTTA16170.1128375181607694No Hit
CCACTGGTGTTCCTCCAAATATCTACGCATTCCACCGCTACACTTGGAAT16090.11227926204123559No Hit
GCCCCCAACACTTAGCACTCATCGTTTACGGCGTGGACTACCAGGGTATC15740.1098368915182752No Hit
CTTTTGAGTTTCAACCTTGCGGTCGTAATCCCCAGGCGGAGTGCTTAATA15680.10941819942862487No Hit
CTCATCGTTTACGGCGTGGACTACCAGGGTATCTAATCCTGTTCGCTCCC15620.10899950733897451No Hit
GGCTAACTACGTGCCAGCAGCCGCGGTAATACGTAGGTGACAAGCGTTGT15580.10872037927920761No Hit
ACATTCAAGTCTGTCAGTTTCCAATGCCGTTCCACGGTTAAGCCGTGGGC15110.10544062457694653No Hit
TACGTAGGTGACAAGCGTTGTCCGGATTTATTGGGCGTAAAGGGAGCGCA14980.10453345838270411No Hit
CCGCTACACTTGGAATTCCATTTTCCTCTTCTACATTCAAGTCTGTCAGT14930.10418454830799548No Hit
GGATGAAGAAACATGTATGGAAGGAATAAACGACTTTTACCGAACATGTA14870.10376585621834514No Hit
CAAGAGCTGGTAAGGTTCTTCGCGTTGCTTCGAATTAAACCACATGCTCC14760.10299825405398616No Hit
ATTCTTTTGAGTTTCAACCTTGCGGTCGTAATCCCCAGGCGGAGTGCTTA14750.10292847203904443No Hit
ACGTTAGCTCCAGCACCGAGGGCCGGAAAGCCCCCAACACTTAGCACTCA14590.10181195979997683No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCTTCC8850.0130.35211145
CCTCGGG301.0970325E-7120.92415145
AACGGCC3350.0116.9535145
CTACACC6150.0114.43553145
TAGAAAC26250.0110.619052
AAACACG25800.0108.638035
AGGCGCC750.0106.413246145
GAAACAC26650.0105.705494
TAGTAAC26350.0103.054152
TCCACCC2050.0102.638054145
TAGCGGG650.0100.46006145
GTAGAAA29450.099.5828551
ATAAGCC301.5937394E-596.73932145
CACGCAC1300.094.693553
AACACGA19300.092.692676
ACACGAG20150.088.782567
GCACGCA1400.087.9297262
AGCACGC1400.087.9297261
TGGCGCC259.359607E-487.065384145
AACACGT11700.086.665846
Produced by FastQC (version 0.11.5)