FastQCFastQC Report
Thu 11 Jul 2019
HWC5JBCX2_l01_n02_SM2_1230_P1_DM2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWC5JBCX2_l01_n02_SM2_1230_P1_DM2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1440937
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCTAATCCTGTTCGCTCCCCACGCTTTCGAGCCTCAGCGTCAATGTTAG208291.4455177429686379No Hit
GTTTAGTCTAATGTGAAAGCCCACGGCTTAACCGTGGAACGGCATTGGAA193521.343014996491866No Hit
GCTTAATACGTTAGCTCCAGCACCGAGGGCCGGAAAGCCCCCAACACTTA163961.1378707049648944No Hit
GTAATACGTAGGTGACAAGCGTTGTCCGGATTTATTGGGCGTAAAGGGAG132170.9172503725006714No Hit
GTAGTAACAAGAGCTGGTAAGGTTCTTCGCGTTGCTTCGAATTAAACCAC131320.9113514331299702No Hit
CTACTAGCGCCTTGACGGTATCTAACCAGAAAGTCACGGCTAACTACGTG128900.8945568057451506No Hit
GGTAAGGTTCTTCGCGTTGCTTCGAATTAAACCACATGCTCCACCGCTTG100430.6969770364700191No Hit
GTATCTAACCAGAAAGTCACGGCTAACTACGTGCCAGCAGCCGCGGTAAT89320.6198744289306194No Hit
AGGTAACTACTAGCGCCTTGACGGTATCTAACCAGAAAGTCACGGCTAAC88530.6143918852802032No Hit
GTAGAAACACGAGCTAGGTAACTACTAGCGCCTTGACGGTATCTAACCAG85000.5898939370701148No Hit
CACTTAGCACTCATCGTTTACGGCGTGGACTACCAGGGTATCTAATCCTG84950.5895469406365441No Hit
AACAAGAGCTGGTAAGGTTCTTCGCGTTGCTTCGAATTAAACCACATGCT77610.5386078641883719No Hit
CTCCAGCACCGAGGGCCGGAAAGCCCCCAACACTTAGCACTCATCGTTTA76780.532847723391099No Hit
GATTTATTGGGCGTAAAGGGAGCGCAGGCGGTCTGTTTAGTCTAATGTGA72880.5057820015725878No Hit
GTCTAATGTGAAAGCCCACGGCTTAACCGTGGAACGGCATTGGAAACTGA67850.4708741603553799No Hit
ACTTAGCACTCATCGTTTACGGCGTGGACTACCAGGGTATCTAATCCTGT65640.4555369179915569No Hit
GGATTAGATACCCTGGTAGTCCACGCCGTAAACGATGAGTGCTAAGTGTT63410.4400608770543057No Hit
CTCCACCGCTTGTGCGGGTCCCCGTCAATTCTTTTGAGTTTCAACCTTGC60230.4179919038792119No Hit
GCATTGGAAACTGACAGACTTGAATGTAGAAGAGGAAAATGGAATTCCAA54750.37996109475986806No Hit
CTACCAGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGAGCCTCAGCG54270.3766299289975898No Hit
CGGTAATACGTAGGTGACAAGCGTTGTCCGGATTTATTGGGCGTAAAGGG53520.37142498249402994No Hit
CGGCTTAACCGTGGAACGGCATTGGAAACTGACAGACTTGAATGTAGAAG53220.369343003892606No Hit
GGTAACTACTAGCGCCTTGACGGTATCTAACCAGAAAGTCACGGCTAACT51680.3586555137386298No Hit
GTAGAAACACGTGCTAGGTAACTACTAGCGCCTTGACGGTATCTAACCAG50950.35358936580849826No Hit
GTGAAAGCCCACGGCTTAACCGTGGAACGGCATTGGAAACTGACAGACTT50010.34706583285736986No Hit
GAATTAAACCACATGCTCCACCGCTTGTGCGGGTCCCCGTCAATTCTTTT47860.3321449862138317No Hit
GCTTAACCGTGGAACGGCATTGGAAACTGACAGACTTGAATGTAGAAGAG47810.33179798978026104No Hit
GTTCGCTCCCCACGCTTTCGAGCCTCAGCGTCAATGTTAGACCAGAAAAT45510.3158361538360109No Hit
TATCTACGCATTCCACCGCTACACTTGGAATTCCATTTTCCTCTTCTACA45120.3131295816541597No Hit
GCGTAAAGGGAGCGCAGGCGGTCTGTTTAGTCTAATGTGAAAGCCCACGG43140.2993885228847618No Hit
GCGTAGATATTTGGAGGAACACCAGTGGCGAAGGCGATTTTCTGGTCTAA42870.2975147421434802No Hit
CTGGTAAGGTTCTTCGCGTTGCTTCGAATTAAACCACATGCTCCACCGCT42270.2933507849406324No Hit
ATGTTAGACCAGAAAATCGCCTTCGCCACTGGTGTTCCTCCAAATATCTA41550.2883540362972149No Hit
GTAACTACTAGCGCCTTGACGGTATCTAACCAGAAAGTCACGGCTAACTA41110.2853004676817932No Hit
CCTTGACGGTATCTAACCAGAAAGTCACGGCTAACTACGTGCCAGCAGCC39920.27704195256281156No Hit
GGTGACAAGCGTTGTCCGGATTTATTGGGCGTAAAGGGAGCGCAGGCGGT39290.2726697974998213No Hit
CTACGTGCCAGCAGCCGCGGTAATACGTAGGTGACAAGCGTTGTCCGGAT37890.2629538973598429No Hit
GGCGTAAAGGGAGCGCAGGCGGTCTGTTTAGTCTAATGTGAAAGCCCACG37650.26128831447870376No Hit
CCCCCAACACTTAGCACTCATCGTTTACGGCGTGGACTACCAGGGTATCT37320.25899813801713745No Hit
CTGTAGAACATGCTTGTAAATTCAAACATTCCAGTCTCATTACAGTAACT36660.2544177850940048No Hit
GTCGTAATCCCCAGGCGGAGTGCTTAATACGTTAGCTCCAGCACCGAGGG36500.2533073965065787No Hit
CACGTGCTAGGTAACTACTAGCGCCTTGACGGTATCTAACCAGAAAGTCA36280.2517806121988678No Hit
ATTATTATGTGGTCATTCAATACCTCCATGTTGTCAGAGAGCACTTCCTT35580.24692266212887862No Hit
GTGCTTAATACGTTAGCTCCAGCACCGAGGGCCGGAAAGCCCCCAACACT34940.24248110777917425No Hit
GGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGAGCCTCAGCGTCAATG34560.23984393488403724No Hit
GACTACCAGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGAGCCTCAG33940.23554117910776112No Hit
ATGCTCCACCGCTTGTGCGGGTCCCCGTCAATTCTTTTGAGTTTCAACCT33810.23463898838047742No Hit
CTTGAATGTAGAAGAGGAAAATGGAATTCCAAGTGTAGCGGTGGAATGCG32960.2287400490097763No Hit
ATACGTAGGTGACAAGCGTTGTCCGGATTTATTGGGCGTAAAGGGAGCGC32940.22860125043634802No Hit
GAGCTGGTAAGGTTCTTCGCGTTGCTTCGAATTAAACCACATGCTCCACC32610.22631107397478167No Hit
GCTTCGAATTAAACCACATGCTCCACCGCTTGTGCGGGTCCCCGTCAATT32340.22443729323350015No Hit
GCTAACTACGTGCCAGCAGCCGCGGTAATACGTAGGTGACAAGCGTTGTC31660.21971814173693924No Hit
GACTTGAATGTAGAAGAGGAAAATGGAATTCCAAGTGTAGCGGTGGAATG31600.21930174601665445No Hit
GGTTCTTCGCGTTGCTTCGAATTAAACCACATGCTCCACCGCTTGTGCGG31090.21576238239423376No Hit
GCGTCAATGTTAGACCAGAAAATCGCCTTCGCCACTGGTGTTCCTCCAAA30790.2136804037928098No Hit
CACGAGCTAGGTAACTACTAGCGCCTTGACGGTATCTAACCAGAAAGTCA30410.21104323089767285No Hit
GTCCGGATTTATTGGGCGTAAAGGGAGCGCAGGCGGTCTGTTTAGTCTAA29670.2059076836808271No Hit
GACGGTATCTAACCAGAAAGTCACGGCTAACTACGTGCCAGCAGCCGCGG29640.20569948582068476No Hit
GTGTAGCGGTGGAATGCGTAGATATTTGGAGGAACACCAGTGGCGAAGGC28880.2004251400304108No Hit
TAACAAGAGCTGGTAAGGTTCTTCGCGTTGCTTCGAATTAAACCACATGC28120.1951507942401368No Hit
AGTCTAATGTGAAAGCCCACGGCTTAACCGTGGAACGGCATTGGAAACTG27730.19244422205828568No Hit
CTTTCGAGCCTCAGCGTCAATGTTAGACCAGAAAATCGCCTTCGCCACTG27130.18828026485543783No Hit
GTAAGAAATACAGTAAAATTGAATTTAATGCAATTCTTCTATCTCCAAAA27110.18814146628200956No Hit
GTAACAAGAGCTGGTAAGGTTCTTCGCGTTGCTTCGAATTAAACCACATG26680.1851572969533019No Hit
GAATACAACAATAACCTCTTTTAGGTTGAATGATTTAAATGGAGCCGACA26220.1819649297644519No Hit
CGTTAGCTCCAGCACCGAGGGCCGGAAAGCCCCCAACACTTAGCACTCAT24780.17197143247761698No Hit
TCGTTTACGGCGTGGACTACCAGGGTATCTAATCCTGTTCGCTCCCCACG24740.17169383533076046No Hit
CATCGTTTACGGCGTGGACTACCAGGGTATCTAATCCTGTTCGCTCCCCA24710.1714856374706181No Hit
ATGTAGAAGAGGAAAATGGAATTCCAAGTGTAGCGGTGGAATGCGTAGAT24180.16780747527476914No Hit
ATCCTGTTCGCTCCCCACGCTTTCGAGCCTCAGCGTCAATGTTAGACCAG24170.167738075988055No Hit
GTTCTTCGCGTTGCTTCGAATTAAACCACATGCTCCACCGCTTGTGCGGG23910.16593369453348758No Hit
GATATTTGGAGGAACACCAGTGGCGAAGGCGATTTTCTGGTCTAACATTG23800.16517030237963215No Hit
GCCCACGGCTTAACCGTGGAACGGCATTGGAAACTGACAGACTTGAATGT23520.16322712235163647No Hit
CCATTAGAAAGATATAATGAAGAAACAAGGGCAAAATTGAAGAAGCTAAA23300.16170033804392558No Hit
GTTTAATATGCTATCTACCGTGTTGGGAGTAGCTGCACTAGGTATCAAGA22800.15823037370821902No Hit
CCCCAGGCGGAGTGCTTAATACGTTAGCTCCAGCACCGAGGGCCGGAAAG22540.1564259922536516No Hit
ATTTGGAGGAACACCAGTGGCGAAGGCGATTTTCTGGTCTAACATTGACG22500.1561483951067951No Hit
GTAAGGTTCTTCGCGTTGCTTCGAATTAAACCACATGCTCCACCGCTTGT22070.15316422577808744No Hit
CGGCTAACTACGTGCCAGCAGCCGCGGTAATACGTAGGTGACAAGCGTTG22020.1528172293445168No Hit
GTGCTAAGTGTTGGGGGCTTTCCGGCCCTCGGTGCTGGAGCTAACGTATT21900.15198443790394722No Hit
CCCCAACACTTAGCACTCATCGTTTACGGCGTGGACTACCAGGGTATCTA21880.15184563933051898No Hit
TTCCACCGCTACACTTGGAATTCCATTTTCCTCTTCTACATTCAAGTCTG21790.15122104575009176No Hit
CCCTGGTAGTCCACGCCGTAAACGATGAGTGCTAAGTGTTGGGGGCTTTC21600.1499024593025233No Hit
CCGTGGAACGGCATTGGAAACTGACAGACTTGAATGTAGAAGAGGAAAAT21510.14927786572209611No Hit
AGCTAGGTAACTACTAGCGCCTTGACGGTATCTAACCAGAAAGTCACGGC21310.14788987998781347No Hit
ACTAGCGCCTTGACGGTATCTAACCAGAAAGTCACGGCTAACTACGTGCC21300.14782048070109935No Hit
GTTTAGTATACCATTAGAAAGATATAATGAAGAAACAAGGGCAAAATTGA21280.14768168212767108No Hit
TCCCCAGGCGGAGTGCTTAATACGTTAGCTCCAGCACCGAGGGCCGGAAA21260.14754288355424283No Hit
GCGTTGTCCGGATTTATTGGGCGTAAAGGGAGCGCAGGCGGTCTGTTTAG21090.1463630956801026No Hit
CATTGACGCTGAGGCTCGAAAGCGTGGGGAGCGAACAGGATTAGATACCC20870.1448363113723917No Hit
GTGCGGGTCCCCGTCAATTCTTTTGAGTTTCAACCTTGCGGTCGTAATCC20590.14289313134439605No Hit
CCACATGCTCCACCGCTTGTGCGGGTCCCCGTCAATTCTTTTGAGTTTCA20090.1394231670086895No Hit
GCTCCACCGCTTGTGCGGGTCCCCGTCAATTCTTTTGAGTTTCAACCTTG20030.1390067712884047No Hit
GGAAAATGGAATTCCAAGTGTAGCGGTGGAATGCGTAGATATTTGGAGGA19900.138104580561121No Hit
AGTAACAAGAGCTGGTAAGGTTCTTCGCGTTGCTTCGAATTAAACCACAT19420.1347734147988427No Hit
TATCTAACCAGAAAGTCACGGCTAACTACGTGCCAGCAGCCGCGGTAATA19330.1341488212184155No Hit
CCAAATATCTACGCATTCCACCGCTACACTTGGAATTCCATTTTCCTCTT19310.13401002264498726No Hit
CACCAGTGGCGAAGGCGATTTTCTGGTCTAACATTGACGCTGAGGCTCGA19150.13289963405756117No Hit
GAATGTAGAAGAGGAAAATGGAATTCCAAGTGTAGCGGTGGAATGCGTAG19060.13227504047713398No Hit
TTCCAATGCCGTTCCACGGTTAAGCCGTGGGCTTTCACATTAGACTAAAC19050.13220564119041986No Hit
GTCTAACATTGACGCTGAGGCTCGAAAGCGTGGGGAGCGAACAGGATTAG18990.13178924547013504No Hit
CCTCCAAATATCTACGCATTCCACCGCTACACTTGGAATTCCATTTTCCT18740.1300542633022818No Hit
GACAAGCGTTGTCCGGATTTATTGGGCGTAAAGGGAGCGCAGGCGGTCTG18680.129637867581997No Hit
CCCCACGCTTTCGAGCCTCAGCGTCAATGTTAGACCAGAAAATCGCCTTC18650.1294296697218546No Hit
GGTCTGTTTAGTCTAATGTGAAAGCCCACGGCTTAACCGTGGAACGGCAT18450.12804168398757196No Hit
ATTAGTAGTAACAAGAGCTGGTAAGGTTCTTCGCGTTGCTTCGAATTAAA18400.12769468755400132No Hit
TATCTATAATGCTCGAACCATTTCAGATTCTTACAATTTGTTCTTTTATC18280.12686189611343177No Hit
ACGTAGGTGACAAGCGTTGTCCGGATTTATTGGGCGTAAAGGGAGCGCAG18200.1263067018197187No Hit
GTATCTAATCCTGTTCGCTCCCCACGCTTTCGAGCCTCAGCGTCAATGTT17930.12443292107843715No Hit
CCAGTGGCGAAGGCGATTTTCTGGTCTAACATTGACGCTGAGGCTCGAAA17880.12408592464486651No Hit
AATTAAACCACATGCTCCACCGCTTGTGCGGGTCCCCGTCAATTCTTTTG17810.12360012963786758No Hit
CGTAAAGGGAGCGCAGGCGGTCTGTTTAGTCTAATGTGAAAGCCCACGGC17630.12235094247701322No Hit
TTATTATTGTCATTCCTATTGCCATATCTGCTGATTCATTTACTCCAGCA17570.12193454675672843No Hit
GTCACGGCTAACTACGTGCCAGCAGCCGCGGTAATACGTAGGTGACAAGC17490.12137935246301539No Hit
GTTCTACAGAGATGGATTTGTATCTAATTTTGCAATGGAACTCCCTTCGT17380.12061596030915996No Hit
ACCACATGCTCCACCGCTTGTGCGGGTCCCCGTCAATTCTTTTGAGTTTC17150.11901977671473492No Hit
GCGGTGGAATGCGTAGATATTTGGAGGAACACCAGTGGCGAAGGCGATTT17050.11832578384759361No Hit
GTGCTAGGTAACTACTAGCGCCTTGACGGTATCTAACCAGAAAGTCACGG16690.1158274095258849No Hit
CAATTATTATGTGGTCATTCAATACCTCCATGTTGTCAGAGAGCACTTCC16610.11527221523217185No Hit
GTTTCAACCTTGCGGTCGTAATCCCCAGGCGGAGTGCTTAATACGTTAGC16450.11416182664474575No Hit
ACACTTAGCACTCATCGTTTACGGCGTGGACTACCAGGGTATCTAATCCT16440.11409242735803161No Hit
AACACGAGCTAGGTAACTACTAGCGCCTTGACGGTATCTAACCAGAAAGT16400.11381483021117508No Hit
TATTAGTAGTAACAAGAGCTGGTAAGGTTCTTCGCGTTGCTTCGAATTAA16400.11381483021117508No Hit
AACCAGAAAGTCACGGCTAACTACGTGCCAGCAGCCGCGGTAATACGTAG16330.11332903520417617No Hit
AGATTATACTTCAATACTATTTCTGGGATATGCAAGTTTCTCAAATTGTA16290.11305143805731964No Hit
GAGTTTCAACCTTGCGGTCGTAATCCCCAGGCGGAGTGCTTAATACGTTA16250.11277384091046311No Hit
CCACTGGTGTTCCTCCAAATATCTACGCATTCCACCGCTACACTTGGAAT16150.1120798480433218No Hit
GCCCCCAACACTTAGCACTCATCGTTTACGGCGTGGACTACCAGGGTATC15830.10985907086846962No Hit
CTTTTGAGTTTCAACCTTGCGGTCGTAATCCCCAGGCGGAGTGCTTAATA15700.10895688014118592No Hit
CTCATCGTTTACGGCGTGGACTACCAGGGTATCTAATCCTGTTCGCTCCC15690.10888748085447178No Hit
GGCTAACTACGTGCCAGCAGCCGCGGTAATACGTAGGTGACAAGCGTTGT15630.10847108513418699No Hit
ACATTCAAGTCTGTCAGTTTCCAATGCCGTTCCACGGTTAAGCCGTGGGC15150.1051399193719087No Hit
CCGCTACACTTGGAATTCCATTTTCCTCTTCTACATTCAAGTCTGTCAGT15030.10430712793133912No Hit
TACGTAGGTGACAAGCGTTGTCCGGATTTATTGGGCGTAAAGGGAGCGCA15010.10416832935791086No Hit
GGATGAAGAAACATGTATGGAAGGAATAAACGACTTTTACCGAACATGTA14920.10354373577748369No Hit
ATTCTTTTGAGTTTCAACCTTGCGGTCGTAATCCCCAGGCGGAGTGCTTA14840.10298854148377062No Hit
CAAGAGCTGGTAAGGTTCTTCGCGTTGCTTCGAATTAAACCACATGCTCC14830.10291914219705652No Hit
ACGTTAGCTCCAGCACCGAGGGCCGGAAAGCCCCCAACACTTAGCACTCA14670.1018087536096304No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGCGCC700.0134.72552145
TCCACCC2250.0128.968145
GCCTTCC10050.0126.32128145
CTAACGG351.895387E-9124.362015145
CTACACC6250.0121.87477145
AACGGCC4250.0112.65735145
GACCCCC203.856977E-4108.816765145
ATCGAGT203.8815683E-4108.643063
TAGAAAC24150.0102.862612
AAACACG23500.0102.64375
ACGAGCC950.099.137049
TAGTAAC25050.097.432252
GAAACAC25000.096.7681054
AGCACGC1150.094.465671
GTAGAAA27050.092.638051
GCCCTCC554.1472958E-1092.32937145
GCCGACC406.096525E-790.68064145
CACGCAC1200.090.535883
AACACGA18400.087.398946
TCGAGTA259.4230566E-486.9174654
Produced by FastQC (version 0.11.5)