FastQCFastQC Report
Tue 2 Jul 2019
HWC5JBCX2_l01_n02_SM2_1210_P1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWC5JBCX2_l01_n02_SM2_1210_P1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1809675
Sequences flagged as poor quality0
Sequence length151
%GC39

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATTTACACAGTCTCTCCAAAGCTTCCATAAGAAATTTTCGTTAGCTTT61580.34028209485128547No Hit
GTGTAAATACAATAAGTAATGAGGAAGCGAGTAACGAGTTACAGAAAACC54230.29966706729108816No Hit
ATATTACTCTTAGTGCTTTTCCAAAGGATCCTTTTACTAGTTTTCCTTCT40930.2261732078964455No Hit
GAATAGAGACTCCCAAGTATCTGGCTGATTTGTTTGATTACAAAACCAAA36200.20003591805158386No Hit
GATATATCCCTTAGTCTAGATATTGTCTGTCCAAGTTTAAAATCAATTCC33870.18716067802229683No Hit
CAATAATACAAAAGGCCAAAAACACAATGGCAGAATTTAGTGAAGATCCT33230.18362413140481026No Hit
ATTTCCTTTTATTCATCCATTACTTCATCCACTGATTCCAACACTTTACT32950.1820768922596599No Hit
GTCTAGATATTGTCTGTCCAAGTTTAAAATCAATTCCCTTTTCAACATCT31900.176274745465346No Hit
GTCCAGAGATCCTTAGCTCAAGAGCATGGAATAGAGACTCCCAAGTATCT31120.17196457927528425No Hit
ATATAGACCCAAAAGGAGCAATAGAGAGAAATCTAGCAAGGATGTCTCCC28390.1568789976100681No Hit
GTTTTCATAACCTCCTGGTCTCTCATTTGTTTGAGCACTGCTCTTGCAAA26420.14599306505311727No Hit
GTTTAGGATTGGCTCCCTATTTGTGAGTGGGAGGGAAAAATCTGTGTACT25720.14212496719024134No Hit
GCTTAGCACTCTCCCTTTCCCTTCCTCATCCAATGAGGATTCATTACTTA24780.13693066434580795No Hit
CTGTAGAACATGCTTGTAAATTCAAACATTCCAGTCTCATTACAGTAACT23830.13168110296047633No Hit
GTGCTAAGCAGACTCACAGAACTTCAGGCTGAATTAAGTCTGAAAAATTT23750.1312390346332905No Hit
ATATTGTCTATGTAGCTCCTCATTCCTTCAAAATTGGAGAAACCGGCTGG23690.13090748338790115No Hit
ATGTTAATCCATCCCCTGTGGCCCATTTGGCATAATTGGTTTTCTGTAAC23590.13035489797891886No Hit
CTTTTATTACAAGAAACTTCCAGACTACAATAATACAAAAGGCCAAAAAC23240.1284208490474809No Hit
TCCTTGTATTGATGATTCCTGTTCAACAATTGCCTCCATTTGTTGCATTG23230.1283655905065827No Hit
ATCTAGAGGTTTGCTATGTAATAAGTGACATGAATTTTCTTGACGAAGAA23160.1279787807202951No Hit
GAATACAACAATAACCTCTTTTAGGTTGAATGATTTAAATGGAGCCGACA23130.1278130050976004No Hit
GTAATAAGTGACATGAATTTTCTTGACGAAGAAGGAAAAGCATATACAGC22350.12350283890753865No Hit
ATCCTAAACACAGTATATTTTGGCCACTTTCCTGAGTCCACTCTGGGATC22310.12328180474394575No Hit
GTACTACTAAATTCAAAAGTTACAACTGTTACAACATCAGTATCTCCCCT22280.12311602912125105No Hit
TAGTAGTACAGATCCCAGAGTGGACTCAGGAAAGTGGCCAAAATATACTG21660.11968999958556095No Hit
CTCCAAAGCTTCCATAAGAAATTTTCGTTAGCTTTCTCGCTTTTCATTAT21210.1172033652451407No Hit
CCCGTAGAGCATGTAGGAAGTGAAAGAAGGAAATACTTTGTCAACGAAAT20960.11582190172268501No Hit
CTCTACGGGTGCTATGTCTGGCCCTATTTCTGGTAGGTCCAGAGCTCTTT20150.11134595990992859No Hit
CTCCTAGACTCGGCACTAAACCCCTCCAATTGGGCATTTCCAAATACATA20140.11129070136903034No Hit
GGTCTATATTGTCTATGTAGCTCCTCATTCCTTCAAAATTGGAGAAACCG19740.10908035973310126No Hit
ATTCAATATCTGCGTCCATCTAGAGGTTTGCTATGTAATAAGTGACATGA19720.1089698426513048No Hit
GCATTAGAAGGACAAGGGAAAGAACAAAATTTGAGACCACAATATGAAGT19460.10753312058795086No Hit
GATTTATAGAAGTTGGAATAACAAAGGGATTGGCTGATGATTACTTTTGG19410.10725682788345975No Hit
TATCTAGACTAAGGGATATATCTGTTCCAGCCGGTTTCTCCAATTTTGAA19340.10687001809717214No Hit
AGCATATACAGCATTAGAAGGACAAGGGAAAGAACAAAATTTGAGACCAC19340.10687001809717214No Hit
ATATGGAACTTCTGGTAGCTCATGGTCGTAAATGTGAGGCCCTATTGGTC19130.10570958873830938No Hit
GATTAACATACCAGAAAATAATGAAAGAAGTAGCAATAGATGACGAAACA19100.10554381311561468No Hit
TCCAAATACATAGTGCATCAAGCATTTAGTAAATATTACTCTTAGTGCTT18780.1037755398068714No Hit
GTGTTTAGGATTGGCTCCCTATTTGTGAGTGGGAGGGAAAAATCTGTGTA18710.10338873002058381No Hit
GTCTTGATTGTAGCTGAATATCATCAGTTCCATGCTATTTCCCAATTTTT18680.10322295439788912No Hit
CTTCTAATGTCTGATGAACTAGGATTGGCCAATATGACTGAGGGAAAGTC18470.10206252503902635No Hit
ATATAATGCCTTTCTTCTAATGTCTGATGAACTAGGATTGGCCAATATGA18450.10195200795722989No Hit
GTAATGAGGAAGCGAGTAACGAGTTACAGAAAACCAATTATGCCAAATGG18220.10068106151657065No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCGACC151.2265694E-4145.10669145
CGGACAC100.007108592144.882165
TGGCGCC203.855271E-4108.83002145
CGCACCC203.855271E-4108.83002145
GCCGACC352.7505848E-7103.64764145
ATAAGCC352.7505848E-7103.64764145
TTCGTCC607.2759576E-1296.73779145
CGACGAA554.1472958E-1092.34062145
GAAACAC5500.088.241524
GCACGAA259.3605934E-487.06402145
AAACACG5700.085.150045
GCTGCCC1800.084.64557145
AACACGA3100.084.127446
ACGGGAA900.080.61482145
ACACGAG3250.080.244647
AACACGT2800.080.2048346
ACGTGCC1150.075.5990459
ACACGTG3050.073.630677
CGGCACC300.001930355672.553345145
TGACGCC1000.072.553345145
Produced by FastQC (version 0.11.5)