FastQCFastQC Report
Thu 11 Jul 2019
HWC5JBCX2_l01_n02_SM2_1210_P1_DM2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWC5JBCX2_l01_n02_SM2_1210_P1_DM2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1816951
Sequences flagged as poor quality0
Sequence length151
%GC39

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATTTACACAGTCTCTCCAAAGCTTCCATAAGAAATTTTCGTTAGCTTT61760.33991010214364614No Hit
GTGTAAATACAATAAGTAATGAGGAAGCGAGTAACGAGTTACAGAAAACC54340.2990724570998337No Hit
ATATTACTCTTAGTGCTTTTCCAAAGGATCCTTTTACTAGTTTTCCTTCT40970.2254876438605114No Hit
GAATAGAGACTCCCAAGTATCTGGCTGATTTGTTTGATTACAAAACCAAA36320.1998953191362893No Hit
GATATATCCCTTAGTCTAGATATTGTCTGTCCAAGTTTAAAATCAATTCC33930.1867414146006139No Hit
CAATAATACAAAAGGCCAAAAACACAATGGCAGAATTTAGTGAAGATCCT33300.18327406737991284No Hit
ATTTCCTTTTATTCATCCATTACTTCATCCACTGATTCCAACACTTTACT33030.18178806142818382No Hit
GTCTAGATATTGTCTGTCCAAGTTTAAAATCAATTCCCTTTTCAACATCT31990.1760641866511535No Hit
GTCCAGAGATCCTTAGCTCAAGAGCATGGAATAGAGACTCCCAAGTATCT31200.17171624331090932No Hit
ATATAGACCCAAAAGGAGCAATAGAGAGAAATCTAGCAAGGATGTCTCCC28500.1568561837936191No Hit
GTTTTCATAACCTCCTGGTCTCTCATTTGTTTGAGCACTGCTCTTGCAAA26500.14584873229933004No Hit
GTTTAGGATTGGCTCCCTATTTGTGAGTGGGAGGGAAAAATCTGTGTACT25780.14188604976138597No Hit
GCTTAGCACTCTCCCTTTCCCTTCCTCATCCAATGAGGATTCATTACTTA24820.13660247304412723No Hit
CTGTAGAACATGCTTGTAAATTCAAACATTCCAGTCTCATTACAGTAACT23860.13131889632686847No Hit
GTGCTAAGCAGACTCACAGAACTTCAGGCTGAATTAAGTCTGAAAAATTT23820.1310987472969827No Hit
ATATTGTCTATGTAGCTCCTCATTCCTTCAAAATTGGAGAAACCGGCTGG23760.13076852375215403No Hit
ATGTTAATCCATCCCCTGTGGCCCATTTGGCATAATTGGTTTTCTGTAAC23660.13021815117743957No Hit
TCCTTGTATTGATGATTCCTGTTCAACAATTGCCTCCATTTGTTGCATTG23290.1281817726509961No Hit
CTTTTATTACAAGAAACTTCCAGACTACAATAATACAAAAGGCCAAAAAC23260.12801666087858177No Hit
ATCTAGAGGTTTGCTATGTAATAAGTGACATGAATTTTCTTGACGAAGAA23250.1279616236211103No Hit
GAATACAACAATAACCTCTTTTAGGTTGAATGATTTAAATGGAGCCGACA23180.12757636281881019No Hit
GTAATAAGTGACATGAATTTTCTTGACGAAGAAGGAAAAGCATATACAGC22400.12328345673603745No Hit
ATCCTAAACACAGTATATTTTGGCCACTTTCCTGAGTCCACTCTGGGATC22350.12300827044868023No Hit
GTACTACTAAATTCAAAAGTTACAACTGTTACAACATCAGTATCTCCCCT22340.12295323319120878No Hit
TAGTAGTACAGATCCCAGAGTGGACTCAGGAAAGTGGCCAAAATATACTG21760.11976107225786498No Hit
CTCCAAAGCTTCCATAAGAAATTTTCGTTAGCTTTCTCGCTTTTCATTAT21300.11722935841417849No Hit
CCCGTAGAGCATGTAGGAAGTGAAAGAAGGAAATACTTTGTCAACGAAAT21060.1159084642348638No Hit
CTCTACGGGTGCTATGTCTGGCCCTATTTCTGGTAGGTCCAGAGCTCTTT20200.1111752600923195No Hit
CTCCTAGACTCGGCACTAAACCCCTCCAATTGGGCATTTCCAAATACATA20180.11106518557737662No Hit
GGTCTATATTGTCTATGTAGCTCCTCATTCCTTCAAAATTGGAGAAACCG19790.10891873253599024No Hit
ATTCAATATCTGCGTCCATCTAGAGGTTTGCTATGTAATAAGTGACATGA19780.1088636952785188No Hit
GCATTAGAAGGACAAGGGAAAGAACAAAATTTGAGACCACAATATGAAGT19540.10754280109920411No Hit
GATTTATAGAAGTTGGAATAACAAAGGGATTGGCTGATGATTACTTTTGG19480.10721257755437544No Hit
TATCTAGACTAAGGGATATATCTGTTCCAGCCGGTTTCTCCAATTTTGAA19410.10682731675207531No Hit
AGCATATACAGCATTAGAAGGACAAGGGAAAGAACAAAATTTGAGACCAC19370.10660716772218953No Hit
GATTAACATACCAGAAAATAATGAAAGAAGTAGCAATAGATGACGAAACA19210.10572657160264642No Hit
ATATGGAACTTCTGGTAGCTCATGGTCGTAAATGTGAGGCCCTATTGGTC19200.10567153434517497No Hit
TCCAAATACATAGTGCATCAAGCATTTAGTAAATATTACTCTTAGTGCTT18900.1040204166210316No Hit
GTGTTTAGGATTGGCTCCCTATTTGTGAGTGGGAGGGAAAAATCTGTGTA18750.10319485775895992No Hit
GTCTTGATTGTAGCTGAATATCATCAGTTCCATGCTATTTCCCAATTTTT18740.10313982050148848No Hit
CTTCTAATGTCTGATGAACTAGGATTGGCCAATATGACTGAGGGAAAGTC18530.10198403809458813No Hit
ATATAATGCCTTTCTTCTAATGTCTGATGAACTAGGATTGGCCAATATGA18490.10176388906470235No Hit
GTAATGAGGAAGCGAGTAACGAGTTACAGAAAACCAATTATGCCAAATGG18300.10071818117274489No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCACCC100.0070717935145.1339145
ACGTGAC100.0071115186144.862239
GGTACCC403.274181E-11126.99216145
GCTAACC406.0859566E-790.70868145
AAACACG5000.088.3416445
ACACGAG3100.086.4476557
AACACGA3100.086.440526
GAAACAC5050.086.028354
AGTAGCC608.240022E-1084.661446145
CACGAGC3050.083.115388
GGATCCC353.4223285E-582.933655145
ACGTGCA1650.079.015769
AACACGT2250.077.251356
TAGAAAC6250.076.4622
CGACGAA1100.072.56695145
GGACTCC300.001928921272.56695145
GCCGACC702.7939677E-972.56695145
TATCGGG406.634243E-572.56695145
CACGTGC2250.070.819598
TCTGACC1100.065.969955145
Produced by FastQC (version 0.11.5)