FastQCFastQC Report
Thu 11 Jul 2019
HWC5JBCX2_l01_n02_SM2_1208_P1_DM2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWC5JBCX2_l01_n02_SM2_1208_P1_DM2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1578780
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTAGAACATGCTTGTAAATTCAAACATTCCAGTCTCATTACAGTAACT41610.263557937141337No Hit
GTTCTACAGAGATGGATTTGTATCTAATTTTGCAATGGAACTCCCTTCGT29290.18552299877120307No Hit
TCATAGATGTGCCCGTACAGGCAGCAATTTCAACAACATTCCCATACACT28480.1803924549335563No Hit
CCATTAGAAAGATATAATGAAGAAACAAGGGCAAAATTGAAGAAGCTAAA28080.17785885303842208No Hit
GTTGTATTCAGTGCCGTTGCAGCAGGTTGGTTTCTGCATACTGTCCAATC27860.17646537199609824No Hit
GAATACAACAATAACCTCTTTTAGGTTGAATGATTTAAATGGAGCCGACA27760.1758319715223147No Hit
GTTTAATATGCTATCTACCGTGTTGGGAGTAGCTGCACTAGGTATCAAGA27500.17418513029047747No Hit
GTGTAATACCACTGACAACAACACCCACCAAATCTCATTTTGCAAATCTC24930.1579067381142401No Hit
GTATAGCACCGGTCCTGTTCTCCAATAAGATAGCAAGATTGGGGAAAGGA24240.15353627484513357No Hit
CCCATACACTGGTGTTCCCCCTTATTCCCATGGAACAGGAACAGGTTACA23830.15093933290262102No Hit
GTATAGTATGATTATCCAATCCATCGTCATTTAAAGATGCAGCAGTAATA23480.1487224312443786No Hit
ATACTAAACAGATCAGGACAAGGTATTTGAGCCTTTAGTCTTTTTGTTTT22570.14295848693294824No Hit
TTATTATTGTCATTCCTATTGCCATATCTGCTGATTCATTTACTCCAGCA20950.13269739925765464No Hit
TCTGCATACTGTCCAATCAAAAGTCTGTCTCCCCTGGGTTAATTTGTCTA19470.12332307224565803No Hit
GTATAATCTAATGGACCCTGAATACAAAGGGCGGTTACTTCATCCTCAAA19330.12243631158236107No Hit
GCTTATATGTACCCAATCTCACCAAGGTGAGCCATTGCTTTCTCAAAATC19180.12148621087168573No Hit
GTTTAGTATACCATTAGAAAGATATAATGAAGAAACAAGGGCAAAATTGA19150.12129619072955065No Hit
GTGCTATACTACAAAAGTCCCTGAACCAAACTGGGCTGTCTCTGGTTATT19110.12104283054003724No Hit
GGATGAAGAAACATGTATGGAAGGAATAAACGACTTTTACCGAACATGTA18870.11952266940295671No Hit
GTAGTAACAAGAGCATTTTTCAATAACGTTTCTTTGTAATGACAACAAGC18770.11888926892917315No Hit
GTTTTCATAACCTCCTGGTCTCTCATTTGTTTGAGCACTGCTCTTGCAAA18580.1176858080289844No Hit
AGATTATACTTCAATACTATTTCTGGGATATGCAAGTTTCTCAAATTGTA18370.11635566703403893No Hit
ATAATAAACAGTGAAGATGAACATCTCTTGGCGCTTGAGAGAAAGCTGAA18150.11496218599171511No Hit
TTCTAATGGTATACTAAACAGATCAGGACAAGGTATTTGAGCCTTTAGTC17910.1134420248546346No Hit
GCACTATACCTTTTCCTTTTCACTACTTTAACTGGTTTTCCTTGATAAAG17350.10989498220144668No Hit
TTCTTAATCTGTGGGCCATAGCCTCAAGCATGCTATGTTGACCCACTGGC17340.10983164215406832No Hit
TCTTCATCCTTTGCATTAACAAATAGAGCAAAATCATCAGAAGATTGCAG17200.10894488149077136No Hit
ACCTAAAAGAGGTTATTGTTGTATTCAGTGCCGTTGCAGCAGGTTGGTTT16940.10729804025893411No Hit
GTAATATTCAGTGAATCAAAGGTGGGGAGAGAAAATTCTCCTGCATCAAA16860.10679131987990727No Hit
CTGTTAAAACAGGCCTCAAATAGGTTGCAACATTTAGCGTAGCATTGTTC16670.10558785897971852No Hit
CTTCATAGATGTGCCCGTACAGGCAGCAATTTCAACAACATTCCCATACA16600.10514447864807003No Hit
ATTCAATGATGACCAATAACCCCATAAACATCTTCGAAGCTTATATGTAC16600.10514447864807003No Hit
GTATAGATCTGTTTCTTTTGGTTCTCCAACTATGAGTTCCAGACACCGCA16330.10343429736885443No Hit
TCTCAGAGCTGATACAATAAGCTCACAAATAGAACTCGCAGTCCTGCTTT16090.10191413623177391No Hit
ATTTCCTTTTATTCATCCATTACTTCATCCACTGATTCCAACACTTTACT16020.1014707559001254No Hit
GTTAAAACAGGCCTCAAATAGGTTGCAACATTTAGCGTAGCATTGTTCCT16010.10140741585274707No Hit
GTATTTACACAGTCTCTCCAAAGCTTCCATAAGAAATTTTCGTTAGCTTT15960.10109071561585528No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTACCC458.367351E-11112.850586145
TGGCGCC404.794856E-9108.820206145
GCACGCA501.9645086E-10101.430442
AGCACGC501.9645086E-10101.430441
GTAACAA8750.095.226444
GAAACAC6300.093.1562964
CACGCAC554.1836756E-1092.212413
TGACGCC406.095652E-790.6835145
AAACACG6400.090.568625
TAGAAAC6950.087.565842
TATCGGG259.363719E-487.056175145
AACGGCC554.3764885E-879.141975145
CGACGAA851.8189894E-1276.81427145
TTCGTCC300.001930999672.54681145
GCCGACC607.998824E-872.54681145
TGACCCC300.001930999672.54681145
ACACGAG5700.072.459497
AACACGA5700.072.459496
CGGAGCG300.001940735472.45495
ACGAGCC3350.069.2150349
Produced by FastQC (version 0.11.5)