FastQCFastQC Report
Thu 11 Jul 2019
HWC5JBCX2_l01_n01_undetermined.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWC5JBCX2_l01_n01_undetermined.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13780226
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACGCAC7050.094.404853
CGCACTT9550.071.158845
ACGCACT10200.066.637754
ACACGTG2200.061.1264047
AAACACG7200.056.040675
GCACGCA12150.054.785872
GCCGCCC552.8795778E-453.935593145
ACACGAG4750.053.642237
TAGTAAC15600.052.656312
AACAACG5600.044.2362147
CTCTAGC2350.042.2245641
CACGTGC3400.041.6340838
CGGAGCG1202.3836401E-741.2931255
CACGAGC6200.041.096878
GTGCCCG1559.385985E-1041.096879
ACGAGCC3100.038.813719
GGTGCGT1304.4708395E-738.1112028
GTCGTAC1906.7811925E-933.5732961
ACAACGC7450.032.3013428
GTGTAAA5350.031.7952691
Produced by FastQC (version 0.11.5)