FastQCFastQC Report
Tue 2 Jul 2019
HWC5JBCX2_l01_n01_SM2_DEMUX_P1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWC5JBCX2_l01_n01_SM2_DEMUX_P1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences70
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATGCTAGCCTAGCAGTACCCTGAGTACCGCGGGGTCGGAGACGCCCTGTG34.285714285714286No Hit
CGTATAGAATGCTAGCCTAGCAGTACCCTGAGTACCGCGGGGTCGGAGAC22.857142857142857No Hit
GCGGAGCGATACACGGTGATAGCCCTGTACACGTATAGAATGCTAGCCTA22.857142857142857No Hit
CATCTAATTTCAGTCAAATTGGGCAAAATCCCAACAGTAGAAAACTCTAT11.4285714285714286No Hit
CAGGCTACACAATAGACACCGTGATCAGAACACATGAGTACTCGAACAAA11.4285714285714286No Hit
ATACAGGGGACATCACATTTCTTATCTTTTATTTCGAAGCCAAAGGAATA11.4285714285714286No Hit
GCAGTACCCTGAGTACCGCGGGGTCGGAGACGCCCTGTGGGAATCTGTCA11.4285714285714286No Hit
CACTAGGATGTTCCAAGGAGTGAGGGGTGAAATAAATTTTCTCAATAGAG11.4285714285714286No Hit
GCTCTATGTTGACGATGGTCATTTTGTCAACATCTGTCTCTTATACACAT11.4285714285714286No Hit
GTAGAAACACGAGCTATATCCCAGTTTGATTAGCCTTTCACCCCTACCCT11.4285714285714286No Hit
GCCCAGCGATACACGGTGATAGCCCTGTACACGTATAGAATGCTAGCCTA11.4285714285714286No Hit
GCCTGGCTTTATCCAGCAAGGCCAGGGTGCGGTCGGAGCAAAGGTTGTTT11.4285714285714286No Hit
ACAGACTAAAAAGAAAATTAGAGTCAAGAATAAAGACTCACAACAAAAGT11.4285714285714286No Hit
ATCAAGGGAGCTGAGTTTCCTTTGGTTTCTCCGAATCTATGAGGGCTAAT11.4285714285714286No Hit
CCCTTGGAATGCAGAACCTTCTTCTTGACTCAAGGGGCCTTGCTAAATGA11.4285714285714286No Hit
ACTCCTGAGAGACCGATAGTGAACTAGTACCGTGAGGGAAAGGTGAAAAG11.4285714285714286No Hit
TGTTCAGGCTGTGAACCGTTCTGCAACAAAAGGGGTGACATTTCTTCTCC11.4285714285714286No Hit
ATCACAAGGTTTCGCGTCTACCTCATCTGACTATGCGCCCTATTAAGACT11.4285714285714286No Hit
ATATTACTCTTAGTGCTTTTCCAAAGGATCCTTTTACTAGTTTCCCTTCT11.4285714285714286No Hit
GATCACAAGGTTTCGCGTCTACCTCATCTGACTATGCGCCCTATTAAGAC11.4285714285714286No Hit
ATTTCACCGAGCTCATGGCTGAGACAGCGCCCAGATCGTTACACCATTCG11.4285714285714286No Hit
GTTTCCTATGTCTACAGCAGAGGGACCCAGCATTTTCTTTAGTTTTCTCT11.4285714285714286No Hit
CCCATTCTCATCACAGCTTCTCCAAGCGAATCTCTGTATATTTTCAGAGA11.4285714285714286No Hit
TCTCAGGAGTATTTAGCCTTATCAGATGGTGCTGACAGATTCCCACAGGG11.4285714285714286No Hit
CCGTGAGGGAAAGGTGAAAAGAACCTCGAACAGAGGAGTGAAAAGAACCT11.4285714285714286No Hit
GTTAGAGAGTACTTCCTTCATTGCTTCCTTAGCCTGAATTTCTTTTTGGT11.4285714285714286No Hit
ATTTAGCGTAGCATTGTTCTTCAAGAATCATGTTCCTCTGATCAGTATTT11.4285714285714286No Hit
GCGCAGAGACTTGAAGATGTCTTTGCTGGGAAAAACACAGATCTTGAGGC11.4285714285714286No Hit
CTCATCCATCAGCTCCCACTTTAAGCAGACTTCAGGGATGTGAAGATTCC11.4285714285714286No Hit
ACATGATTCTTGAAGAACAATGCTACGCTAAATGTTGCAATCTTTTTGAG11.4285714285714286No Hit
TCTCAGAGGATATGAAAAGATCAGGTTATCAACCCAAAACGTTATCGATG11.4285714285714286No Hit
AGGCAACACCCTTGGCCTCGACATCAAAACAGCCACTCTTGTTGGGAAAC11.4285714285714286No Hit
GTGAGCGTGAACACAAATCCTAAAATTCCCTTAGTCAGAGGTGACAAGAT11.4285714285714286No Hit
GACCATTCTGATCAGTTCCATCACCATTGTTCCGATTCCTTTGACTGCAG11.4285714285714286No Hit
CCTGAGTACCGCGGGGTCGGAGACGCCCTGTGGGAATCTGTCAGCACCAT11.4285714285714286No Hit
ATACAAGGGAATACAACTTAAAACGAATGCTGAAGACATAGGAACCAAAG11.4285714285714286No Hit
ACACGGTGATAGCCCTGTACACGTATAGAATGCTAGCCTAGCAGTACCCT11.4285714285714286No Hit
GATCCGGTGAGATCCATCTTATCTTATCTCGTGACGAACACCTTATCATC11.4285714285714286No Hit
ATGCTGGATCAGATAGAGTGATGGTATCGCCTTTGGCTGTAACATGGTGG11.4285714285714286No Hit
ATAATACAAAAGGCCAAAAACACAATGGCAGAATTTAGTGAAGATCCTGA11.4285714285714286No Hit
CTGACATCACAGGGTGTACAATGATAGATCCAACAAATGGGCCATTACCT11.4285714285714286No Hit
TAAAGAGACTGAAATGATAAAAATAGAGAGTAACGGAAGTGAGAACTCGA11.4285714285714286No Hit
GGTCTATATTTGGTTCCATTAGCAAGCTTCAAAGGTGTTTTTACCCATAT11.4285714285714286No Hit
GTATGGCAAGTAAGAATGGCTGTTCCTCATTATGTGAATATTCCTCATTA11.4285714285714286No Hit
ATTAGTAGAAACACGAGCTATTTCCCAGTTTGATTAGCCTTTCACCCCTA11.4285714285714286No Hit
GCATTCCTCAGTGTGTTTTACTCTTCCTGTTGGAAATATTTCTTTTATTA11.4285714285714286No Hit
AGCGGAGCCTTTAAGATGAATATAAATCCTTATTTTCTCTTCATAGATGT11.4285714285714286No Hit
CAACTGTAGATTGAAGGCATCAAAGAAGCAGATATAACCCCAGCACATGG11.4285714285714286No Hit
ATGTCAAGCTTTCAGGTAGACTGTTTTCTTTGGCATATTCGCAAGCGATT11.4285714285714286No Hit
CCATTCCACTCAAGTCCTCCGATGAGGACCCCAACTGCATTTTTGACATC11.4285714285714286No Hit
CCATCATACTTTACATACAGTCCCATCCCCATTCTTTTAGTTTTAGACAT11.4285714285714286No Hit
GCTCTGGGTGCTAAGGTCCAAGGCCGAGAGGGAAAGAACCCAGACCATCA11.4285714285714286No Hit
GTACTACTAAATTCGAAAGTTACAACTGTTACAACATCAGTGTCCCCCCT11.4285714285714286No Hit
TTCTTAATCTGTGGGCCATAGCCTCAAGCATGCTATGTTGACCCACTGGC11.4285714285714286No Hit
AACACGAGCTATTTCCCAGTTTGATTAGCCTTTCACCCCTACCCTCAGGT11.4285714285714286No Hit
CATTTAAAGAGTGCGTAACAGCTCTCTAGTCGAGCGATCGGGCGTGGATA11.4285714285714286No Hit
ACCCTGTCATTCTACTTGGAGCTCAGATCCCGGTCAGAGATCGAGCCGCT11.4285714285714286No Hit
GTATTGGCCAGCCATCTTCTGGGGGATTATGCTTTCAAATGCTTCAAATG11.4285714285714286No Hit
TAGTTCTGCTATATACATTTACAACCGCAACTGCAGACACATTATGTATA11.4285714285714286No Hit
TTTCTTGTCTTATGGTAAACAGCCTGGTTTTGATCCTAGCCCTGCTTTCC11.4285714285714286No Hit
GTTCTTTATTATTGTCATTCCTATTACCATATCTGCTGATTCATTTACTC11.4285714285714286No Hit
ATTCCCCATTGATCTATTCCTTTCTTCATCTTCAATTTTCCCTTTTAATG11.4285714285714286No Hit
TCCCCGTGCCTGAGCCGCAGCGGGAGGGCGTCTCCGTGGTCACGATGTCC11.4285714285714286No Hit
GAACAGTGACCACAGAAGCTGCTTTTGGTCTAGTTTGTGCCACTTGTGAA11.4285714285714286No Hit
CCAGTTGAATAGCTTAGTGACACCTCCTTGGCCCCATGGAACGTTATTTC11.4285714285714286No Hit
GTGATAGCCCTGTACACGTATAGAATGCTAGCCTAGCAGTACCCTGAGTA11.4285714285714286No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTACTCT50.0145.04
CTCTTAG50.0145.07
ACTCTTA50.0145.06
CTTAGTG50.0145.09
ATTACTC50.0145.03
TATTACT50.0145.02
TACTCTT50.0145.05
TTGGACC50.0145.0145
ATATTAC50.0145.01
TCTTAGT50.0145.08
Produced by FastQC (version 0.11.5)