FastQCFastQC Report
Thu 11 Jul 2019
HWC5JBCX2_l01_n01_SM2_DEMUX_P1_DM2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWC5JBCX2_l01_n01_SM2_DEMUX_P1_DM2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences126
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATGCTAGCCTAGCAGTACCCTGAGTACCGCGGGGTCGGAGACGCCCTGTG32.380952380952381No Hit
CGTATAGAATGCTAGCCTAGCAGTACCCTGAGTACCGCGGGGTCGGAGAC21.5873015873015872No Hit
GCAGTACCCTGAGTACCGCGGGGTCGGAGACGCCCTGTGGGAATCTGTCA21.5873015873015872No Hit
ATACACGGTGATAGCCCTGTACACGTATAGAATGCTAGCCTAGCAGTACC21.5873015873015872No Hit
GCGGAGCGATACACGGTGATAGCCCTGTACACGTATAGAATGCTAGCCTA21.5873015873015872No Hit
AACACGAGCTATTTCCCAGTTTGATTAGCCTTTCACCCCTACCCTCAGGT21.5873015873015872No Hit
GCACAGCGATACACGGTGATAGCCCTGTACACGTATAGAATGCTAGCCTA21.5873015873015872No Hit
CATCTAATTTCAGTCAAATTGGGCAAAATCCCAACAGTAGAAAACTCTAT10.7936507936507936No Hit
CAATTTCCCCCGATGCAATTGCAGGCACTTTCTTGTGTTCTTAGGATGTT10.7936507936507936No Hit
CTGTGGGAATCTGTCAGCACCATCTGATAAGGCTAAATACTCCTGAGAGA10.7936507936507936No Hit
AACCTGACATGTTCGTATCCTCGGAGAAGGTTAGGCAGCTGTCTAATTTT10.7936507936507936No Hit
CTCTTGAGTGCTCTTAAGGTCAGCTGCCACTGCTACTCCATGTGCCCCAT10.7936507936507936No Hit
GCCCAGCGATACACGGTGATAGCCCTGTACACGTATAGAATGCTAGCCTA10.7936507936507936No Hit
GCCTGGCTTTATCCAGCAAGGCCAGGGTGCGGTCGGAGCAAAGGTTGTTT10.7936507936507936No Hit
CCTTATTTCTGCTGTATCAGTCTCCACGTTTAATTTGACAAAAGGTCTTT10.7936507936507936No Hit
ACAGACTAAAAAGAAAATTAGAGTCAAGAATAAAGACTCACAACAAAAGT10.7936507936507936No Hit
GCAGAGCGATACACGGTGATAGCCCTGTACACGTATAGAATGCTAGCCTA10.7936507936507936No Hit
ATCATCAGCTGTGCTTGTGATTCTAATTCACTCACGTCATTAGCTCCCAT10.7936507936507936No Hit
CCCATTCTCATCACAGCTTCTCCAAGCGAATCTCTGTATATTTTCAGAGA10.7936507936507936No Hit
TCTCAGGAGTATTTAGCCTTATCAGATGGTGCTGACAGATTCCCACAGGG10.7936507936507936No Hit
CCGTGAGGGAAAGGTGAAAAGAACCTCGAACAGAGGAGTGAAAAGAACCT10.7936507936507936No Hit
ACCCTGAGTACCGCGGGGTCGGAGACGCCCTGTGGGAATCTGTCAGCACC10.7936507936507936No Hit
TCTCAGAGGATATGAAAAGATCAGGTTATCAACCCAAAACGTTATCGATG10.7936507936507936No Hit
GTGAGCGTGAACACAAATCCTAAAATTCCCTTAGTCAGAGGTGACAAGAT10.7936507936507936No Hit
GACCATTCTGATCAGTTCCATCACCATTGTTCCGATTCCTTTGACTGCAG10.7936507936507936No Hit
GTGTAATACCACTGACAACAACACCCACCAAATCTCATTTTGCAAATCTC10.7936507936507936No Hit
ACACGGTGATAGCCCTGTACACGTATAGAATGCTAGCCTAGCAGTACCCT10.7936507936507936No Hit
GATCCGGTGAGATCCATCTTATCTTATCTCGTGACGAACACCTTATCATC10.7936507936507936No Hit
ATCTCTTGGCGCTTGAGAGAAAGCTGAAGAAAATGCTGGGCCCCTCTGCT10.7936507936507936No Hit
ATGCTGGATCAGATAGAGTGATGGTATCGCCTTTGGCTGTAACATGGTGG10.7936507936507936No Hit
AGTCTTAATAGGGCGCATAGTCAGATGAGGTAGACGCGAAACCTTGTGAT10.7936507936507936No Hit
ACCTCTATAAGACTCCATGCCGACGCTGTTCCTAAAAACATTTCGGGGAG10.7936507936507936No Hit
CTGACATCACAGGGTGTACAATGATAGATCCAACAAATGGGCCATTACCT10.7936507936507936No Hit
CTTTTCAACGTTTATCGGTTCGGTCCTCCATTACATGTTACTGCAACTTC10.7936507936507936No Hit
AGGAACACTCTGTGCTTTGTGCGAAAAACAAGCATCACATTCACACAGAG10.7936507936507936No Hit
GTAGAAACACGTGCTATTTCCCAGTTTGATTAGCCTTTCACCCCTACCCT10.7936507936507936No Hit
CCATACAACTGTTCATAGCTGATTATAGGTACACATACAAATGCCACAGA10.7936507936507936No Hit
CAACTGTAGATTGAAGGCATCAAAGAAGCAGATATAACCCCAGCACATGG10.7936507936507936No Hit
ATGTCAAGCTTTCAGGTAGACTGTTTTCTTTGGCATATTCGCAAGCGATT10.7936507936507936No Hit
CCATTCCACTCAAGTCCTCCGATGAGGACCCCAACTGCATTTTTGACATC10.7936507936507936No Hit
GCCCTGTACACGTATAGAATGCTAGCCTAGCAGTACCCTGAGTACCGCGG10.7936507936507936No Hit
GTTTATTGTCTCTTTGTTTGGACCTTTTATTCGTATTTTCATGTTTATTC10.7936507936507936No Hit
TTGGTATCCTCTTTATGAGGAAACCCTTTCTGTTTTTGGCAGGCAATTTT10.7936507936507936No Hit
GATATGAGCTCTTGGGGGAGATCAGCCTGTTATCCAGCGTACCTTTTATC10.7936507936507936No Hit
GAAGCAGAGCGATACACGGTGATAGCCCTGTACACGTATAGAATGCTAGC10.7936507936507936No Hit
GTAAATGGCTGTTGCTGCTGAGTGCCAAGTCTCTTTTCCACCATCATGTA10.7936507936507936No Hit
GTACTACTAAATTCGAAAGTTACAACTGTTACAACATCAGTGTCCCCCCT10.7936507936507936No Hit
TGCCAGGAGAAGGTTCCTGCCCGTGGCAGGAGCAACATCAGCTGAGTTCA10.7936507936507936No Hit
TAGTTCTGCTATATACATTTACAACCGCAACTGCAGACACATTATGTATA10.7936507936507936No Hit
TTTCTTGTCTTATGGTAAACAGCCTGGTTTTGATCCTAGCCCTGCTTTCC10.7936507936507936No Hit
GTTCTTTATTATTGTCATTCCTATTACCATATCTGCTGATTCATTTACTC10.7936507936507936No Hit
CCCTGTACACGTATAGAATGCTAGCCTAGCAGTACCCTGAGTACCGCGGG10.7936507936507936No Hit
GAACAGTGACCACAGAAGCTGCTTTTGGTCTAGTTTGTGCCACTTGTGAA10.7936507936507936No Hit
CCAGTTGAATAGCTTAGTGACACCTCCTTGGCCCCATGGAACGTTATTTC10.7936507936507936No Hit
ATTTCCTTTTATTCATCCATTACTTCATCCACTGATTCCAACACTTTACT10.7936507936507936No Hit
CAGGCTACACAATAGACACCGTGATCAGAACACATGAGTACTCGAACAAA10.7936507936507936No Hit
ATACAGGGGACATCACATTTCTTATCTTTTATTTCGAAGCCAAAGGAATA10.7936507936507936No Hit
CATTACAGTAACTTTTCTTTTTGCTCATGTTTATTCCCAATAATTTACAT10.7936507936507936No Hit
CACTAGGATGTTCCAAGGAGTGAGGGGTGAAATAAATTTTCTCAATAGAG10.7936507936507936No Hit
TTATAGAGGTAGAGCTACCGATTGGGTGCGGGGGAGTCAAATCCTACCAA10.7936507936507936No Hit
GCTCTATGTTGACGATGGTCATTTTGTCAACATCTGTCTCTTATACACAT10.7936507936507936No Hit
GAACAGGAACAGGTTACACAATAGACACCGTGCTCAGAACGCATGAGTAC10.7936507936507936No Hit
GTAGAAACACGAGCTATATCCCAGTTTGATTAGCCTTTCACCCCTACCCT10.7936507936507936No Hit
AAACAAGGGTGTTTTTTATCATTAAATAAGCTGAAACGAGAAAGCTCTTA10.7936507936507936No Hit
ATACTAAACAGATCAGGACAAGGTATTTGAGCCTTTAGTCTTTTTGTTTT10.7936507936507936No Hit
AACTAGTACCGTGAGGGAAAGGTGAAAAGAACCTCGAACAGAGGAGTGAA10.7936507936507936No Hit
ATCAAGGGAGCTGAGTTTCCTTTGGTTTCTCCGAATCTATGAGGGCTAAT10.7936507936507936No Hit
CCCTTGGAATGCAGAACCTTCTTCTTGACTCAAGGGGCCTTGCTAAATGA10.7936507936507936No Hit
GCCTTTGCATGTTCCCCTGTGTAGTAAGGCTTGCTTTTGTTTAATCCACC10.7936507936507936No Hit
ACTCCTGAGAGACCGATAGTGAACTAGTACCGTGAGGGAAAGGTGAAAAG10.7936507936507936No Hit
GTATTACTAAGGGCTTTCACCGAAGAGGGAGCAATTGTTGGCGAAATCTC10.7936507936507936No Hit
TGTTCAGGCTGTGAACCGTTCTGCAACAAAAGGGGTGACATTTCTTCTCC10.7936507936507936No Hit
ATCACAAGGTTTCGCGTCTACCTCATCTGACTATGCGCCCTATTAAGACT10.7936507936507936No Hit
ATATTACTCTTAGTGCTTTTCCAAAGGATCCTTTTACTAGTTTCCCTTCT10.7936507936507936No Hit
GATCACAAGGTTTCGCGTCTACCTCATCTGACTATGCGCCCTATTAAGAC10.7936507936507936No Hit
ATTTCACCGAGCTCATGGCTGAGACAGCGCCCAGATCGTTACACCATTCG10.7936507936507936No Hit
GAGTACTTCCTTCATTGCTTCTTTAGCCTGAATTTCTTTTTGGTAACTGT10.7936507936507936No Hit
CCAATCCACTAATCAGGCATGAAAACAGAATGGTGCTGGCCAGCACTACG10.7936507936507936No Hit
GGAATACCAAGAGAATCCACTTGGATACATAGGGAACTGATAAAAGAAAA10.7936507936507936No Hit
GTTTCCTATGTCTACAGCAGAGGGACCCAGCATTTTCTTTAGTTTTCTCT10.7936507936507936No Hit
ATGTTACTGCAACTTCAACCTGCCCAAGGGTAGATCACAAGGTTTCGCGT10.7936507936507936No Hit
GTTAGAGAGTACTTCCTTCATTGCTTCCTTAGCCTGAATTTCTTTTTGGT10.7936507936507936No Hit
ATTTAGCGTAGCATTGTTCTTCAAGAATCATGTTCCTCTGATCAGTATTT10.7936507936507936No Hit
GAAGCAGGCACTTTCTTAAAATGTCGCTGTTTGGAGACACAATTGCCTAC10.7936507936507936No Hit
GCGCAGAGACTTGAAGATGTCTTTGCTGGGAAAAACACAGATCTTGAGGC10.7936507936507936No Hit
CTCATCCATCAGCTCCCACTTTAAGCAGACTTCAGGGATGTGAAGATTCC10.7936507936507936No Hit
ACATGATTCTTGAAGAACAATGCTACGCTAAATGTTGCAATCTTTTTGAG10.7936507936507936No Hit
TCTGCATACTGTCCAATCAAAAGTCTGTCTCCCCTGGGTTAATTTGTCTA10.7936507936507936No Hit
AGGCAACACCCTTGGCCTCGACATCAAAACAGCCACTCTTGTTGGGAAAC10.7936507936507936No Hit
CCTGAGTACCGCGGGGTCGGAGACGCCCTGTGGGAATCTGTCAGCACCAT10.7936507936507936No Hit
ATACAAGGGAATACAACTTAAAACGAATGCTGAAGACATAGGAACCAAAG10.7936507936507936No Hit
GTAGTAACAAGAGCTATTTCCCAGTTTGATTAGCCTTTCACCCCTACCCT10.7936507936507936No Hit
GTGGGAATCTGTCAGCACCATCTGATAAGGCTAAATACTCCTGAGAGACC10.7936507936507936No Hit
ATAATACAAAAGGCCAAAAACACAATGGCAGAATTTAGTGAAGATCCTGA10.7936507936507936No Hit
ACGTAATGTGTGGTCACTCCGTTGGCAGATGAGGTGAACTTCTGGGGCTT10.7936507936507936No Hit
AGCGGTGCGATACACGGTGATAGCCCTGTACACGTATAGAATGCTAGCCT10.7936507936507936No Hit
TAAAGAGACTGAAATGATAAAAATAGAGAGTAACGGAAGTGAGAACTCGA10.7936507936507936No Hit
GGTCTATATTTGGTTCCATTAGCAAGCTTCAAAGGTGTTTTTACCCATAT10.7936507936507936No Hit
GTATGGCAAGTAAGAATGGCTGTTCCTCATTATGTGAATATTCCTCATTA10.7936507936507936No Hit
ATTCAATGATGACCAATAACCCCATAAACATCTTCGAAGCTTATATGTAC10.7936507936507936No Hit
ATTAGTAGAAACACGAGCTATTTCCCAGTTTGATTAGCCTTTCACCCCTA10.7936507936507936No Hit
GCATTCCTCAGTGTGTTTTACTCTTCCTGTTGGAAATATTTCTTTTATTA10.7936507936507936No Hit
AGCGGAGCCTTTAAGATGAATATAAATCCTTATTTTCTCTTCATAGATGT10.7936507936507936No Hit
CCATCATACTTTACATACAGTCCCATCCCCATTCTTTTAGTTTTAGACAT10.7936507936507936No Hit
GCTCTGGGTGCTAAGGTCCAAGGCCGAGAGGGAAAGAACCCAGACCATCA10.7936507936507936No Hit
CTCCTGGGTGATATATTTGTCTCCAATTTTCACCTTGTAAGCAGTGTAGC10.7936507936507936No Hit
ATTGTATCAGCTCTGAGATCATCCACTTTCTCATCTAGTTCTAGTATTTC10.7936507936507936No Hit
TTCTTAATCTGTGGGCCATAGCCTCAAGCATGCTATGTTGACCCACTGGC10.7936507936507936No Hit
CATTTAAAGAGTGCGTAACAGCTCTCTAGTCGAGCGATCGGGCGTGGATA10.7936507936507936No Hit
ACCCTGTCATTCTACTTGGAGCTCAGATCCCGGTCAGAGATCGAGCCGCT10.7936507936507936No Hit
GTGTAGAGTGGCTGCTTGGGTTCAAACAGAGATGAACCTATTGAGCACTC10.7936507936507936No Hit
GAGTGAAAAGAACCTGAAACCGTGTGCTTACAAGCGGTCGGAGCTGGCAG10.7936507936507936No Hit
GTATTGGCCAGCCATCTTCTGGGGGATTATGCTTTCAAATGCTTCAAATG10.7936507936507936No Hit
GATAGCCCTGTACACGTATAGAATGCTAGCCTAGCAGTACCCTGAGTACC10.7936507936507936No Hit
ATTCCCCATTGATCTATTCCTTTCTTCATCTTCAATTTTCCCTTTTAATG10.7936507936507936No Hit
TCCCCGTGCCTGAGCCGCAGCGGGAGGGCGTCTCCGTGGTCACGATGTCC10.7936507936507936No Hit
GGTGAAAACACCCTTGAAGCTGGCCAATGGAACCAAATATAGACCTCCTG10.7936507936507936No Hit
GTGATAGCCCTGTACACGTATAGAATGCTAGCCTAGCAGTACCCTGAGTA10.7936507936507936No Hit

[OK]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers