FastQCFastQC Report
Thu 11 Jul 2019
HWC5JBCX2_l01_n01_SM2_9974_P1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWC5JBCX2_l01_n01_SM2_9974_P1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences272104
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATATACAGAAAGCACTAATTGGTGCCTCTATCTGCTTTTTAAAACCCAA13450.4942962984741128No Hit
ATATAGTAGCGCTGAGCTTTCATGGCCTTCTGCTATTTCAAATGCTTCAT10590.3891894275718108No Hit
CTACTATATTGTCTCATGGTCATGTACCTGAATCCTGGAAATTATTCAAT9060.33296092670449534No Hit
GCTTTAGCACTTCCATTACATCCTTTGCAATTCCTTCCCCATTCTTCTGA8510.3127480669155911No Hit
CATTATAGGTATTTCTTCACAAGAGCTGAATTTCCCATAGAGCTCTGCTT8280.30429541645841296No Hit
GCCTTGGAATGGATAAAAAACAAAAGATGCTTAACTGATATACAGAAAGC8270.3039279099167965No Hit
GTGTGAATGTGATGCTTGTTTTTCGCACAAAGCACAGAGTGTTCCTAGTT7790.2862875959192074No Hit
ACTATATTGTCTCATGGTCATGTACCTGAATCCTGGAAATTATTCAATGC7590.27893746508687856No Hit
GTTCATAGCTGAGACCATCTGCATTTCCCGTCTCACTCCTGGCACTGAGG7270.26717725575515244No Hit
CATTCACACAGAGCTCATAGCAGAGCAGCGAGATCCTCAGTGCCAGGAGT6980.2565195660482757No Hit
GCTATGAGCTCTGTGTGAATGTGATGCTTGTTTTTCGCACAAAGCACAGA6410.23557169317613855No Hit
ATATTGTCTCATGGTCATGTACCTGAATCCTGGAAATTATTCAATGCAAG6320.2322641343015906No Hit
CTATAGAACATGCTTGTAAATTCAAACATTCCAGTTTCGTTACAGTAACT6150.22601652309411105No Hit
CTTCTGACTTGCCCCAAGGGATCTCAATACTCCAATGTTGCTTTGCAGTT5900.21682885955370004No Hit
CCCCTAGACCAAATGATGGAAGCATAACAGGGCCTTGCGAATCTGATGGA5580.20506865022197396No Hit
GTAAGAAATACAGTAAAAATTGAATTTAATGTGATTCTTCTACCTCCAAA5520.2028636109722753No Hit
GTCCTATTGTCCAAGCCATGAAATGGAGAGCTGATAAAATAAAAGAACAA5460.20065857172257667No Hit
GCTCATAGCAGAGCAGCGAGATCCTCAGTGCCAGGAGTGAGACGGGAAAT5430.19955605209772734No Hit
GTCATGTACCTGAATCCTGGAAATTATTCAATGCAAGTAAAACTAGGAAC5160.18963337547408343No Hit
AAATTACACTGTTGGTTCGGTGGGAAAGAATTTGACCTAGACTCTGCCTT4950.18191573810013817No Hit
GACCTAGACTCTGCCTTGGAATGGATAAAAAACAAAAGATGCTTAACTGA4900.180078205392056No Hit
CTGATATACAGAAAGCACTAATTGGTGCCTCTATCTGCTTTTTAAAACCC4880.1793431923088231No Hit
GCACAGAGTGTTCCTAGTTTTACTTGCATTGAATAATTTCCAGGATTCAG4740.17419810072619293No Hit
ATTACATCCTTTGCAATTCCTTCCCCATTCTTCTGACTTGCCCCAAGGGA4690.17236056801811073No Hit
GCACTAATTGGTGCCTCTATCTGCTTTTTAAAACCCAAAGACCAGGAAAG4500.16537794372739834No Hit
GCTATGAACACAGCAAAAACAATGAATGGAATGGGGAAAGGAGAAGACGT4490.16501043718578193No Hit
GTCTCATGGTCATGTACCTGAATCCTGGAAATTATTCAATGCAAGTAAAA4330.15913033251991887No Hit
ATTCATCACAGAGCCCTTATCGGGAATGGGAACAACAGCAACAAAAAAGA4270.1569252932702202No Hit
GTCTAGGGGTGTCCAAATAAGTCTTTGTGCACATCAATCTTATTTCCGCT4060.14920765589627494No Hit
CTCCAATGTTGCTTTGCAGTTCTTCTGCCAGTTTTTGGACGTCTTCTCCT3910.14369505777202835No Hit
TTCTTAAAATGTCGCTGTTTGGAGACACAATTGCCTACCTGCTTTCATTG3910.14369505777202835No Hit
ACTCTGTGCTTTGTGCGAAAAACAAGCATCACATTCACACAGAGCTCATA3900.1433275512304119No Hit
GAATAAAGGGGCCAAATAAAGAGACAATAAACAGAGAGGTATCAATTTTG3800.1396524858142475No Hit
CTATTGTCCAAGCCATGAAATGGAGAGCTGATAAAATAAAAGAACAAATT3680.1352424073148502No Hit
GTTCTATAGAGATGGATTTGTATCTAACTTTGCAATGGAAATTCCTTCAT3620.13303736806515157No Hit
GTCCAAGCCATGAAATGGAGAGCTGATAAAATAAAAGAACAAATTGAAAG3610.13266986152353513No Hit
GTTCAAACATTATAGGTATTTCTTCACAAGAGCTGAATTTCCCATAGAGC3530.1297298091906036No Hit
GGTTCAAACATTATAGGTATTTCTTCACAAGAGCTGAATTTCCCATAGAG3510.12899479610737072No Hit
AGCACGCACTTTCTTAAAATGTCGCTGTTTGGAGACACAATTGCCTACCT3500.12862728956575425No Hit
CCATTACATCCTTTGCAATTCCTTCCCCATTCTTCTGACTTGCCCCAAGG3460.1271572633992885No Hit
GTATATGCTTCTCCATATTTTATTTTGAGCAATGCATTACTGTCTGGGCC3430.12605474377443918No Hit
GAATAATTTCCAGGATTCAGGTACATGACCATGAGACAATATAGTAGCGC3430.12605474377443918No Hit
TTCTAATGGTATGCTGAACAGATCAGGACAAGGTATTTGAGCCTTTAGTC3290.12090965219180902No Hit
TTCATGGCCTTCTGCTATTTCAAATGCTTCATGAAAGCTCACACATTTTC3280.12054214565019258No Hit
TTCACACAGAGCTCATAGCAGAGCAGCGAGATCCTCAGTGCCAGGAGTGA3250.11943962602534325No Hit
ATGCTTAACTGATATACAGAAAGCACTAATTGGTGCCTCTATCTGCTTTT3250.11943962602534325No Hit
CTTATGTATTGTTCCGTTCCCGATTAATATTTCTTCATCATTTTTGAATC3230.11870461294211038No Hit
CTTTACATACAGTCCCATCCCCATTCTTTGAGTTTTAGACATCGTCCGAG3170.11649957369241172No Hit
TTGCAATTCCTTCCCCATTCTTCTGACTTGCCCCAAGGGATCTCAATACT3150.11576456060917883No Hit
GTATAGTATGGTTATCCAATCCATCATCATTTAAAGATGCAGCAGTAATG3140.11539705406756241No Hit
TAGTAGCGCTGAGCTTTCATGGCCTTCTGCTATTTCAAATGCTTCATGAA3110.11429453444271308No Hit
CAATACTCCAATGTTGCTTTGCAGTTCTTCTGCCAGTTTTTGGACGTCTT3110.11429453444271308No Hit
ATGCATTACTGTCTGGGCCATCAACTCCGATGTAAGTCCATTCTCTACCA3010.11061946902654868No Hit
GCCCTTATCGGGAATGGGAACAACAGCAACAAAAAAGAAGGGCCTGATTC2930.10767941669361715No Hit
AGCAGGCACTTTCTTAAAATGTCGCTGTTTGGAGACACAATTGCCTACCT2880.10584188398553493No Hit
CCATGAGACAATATAGTAGCGCTGAGCTTTCATGGCCTTCTGCTATTTCA2860.10510687090230207No Hit
TTCATGAAGCATTTGAAATAGCAGAAGGCCATGAAAGCTCAGCGCTACTA2780.10216681856937053No Hit
CTTCCATTACATCCTTTGCAATTCCTTCCCCATTCTTCTGACTTGCCCCA2740.10069679240290476No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTAGTGC151.2290354E-4144.99982145
AGCACGC403.274181E-11126.898161
CACGCAC403.274181E-11126.874853
GCACGCA403.274181E-11126.874852
CACGAGC351.897206E-9124.285558
ACGAGCC256.4563937E-6115.999859
GAAACAC458.367351E-11112.777634
AAACACG458.367351E-11112.777635
GAGCAGA203.8601953E-4108.769851
ACACGAG404.802132E-9108.749877
AACACGA404.802132E-9108.749876
TAGAAAC608.258212E-1084.583232
ACGCACT608.258212E-1084.583234
CGCACTT651.5588739E-978.076825
CCAAGCA754.836693E-967.666582
TAAATAG451.1923104E-464.444374
AGCCACT851.3005774E-859.7058078
CAGCCAC902.0421794E-856.3888177
ACGGATA651.0915266E-555.769157145
ATACGAA400.006046201654.3749353
Produced by FastQC (version 0.11.5)