FastQCFastQC Report
Thu 11 Jul 2019
HWC5JBCX2_l01_n01_SM2_9932_2_P1_DM2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWC5JBCX2_l01_n01_SM2_9932_2_P1_DM2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1392831
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATATACAGAAAGCACTAATTGGTGCCTCTATCTGCTTTTTAAAACCCAA112280.8061279509143606No Hit
CTACTATATTGTCTCATGGTCATGTACCTGAATCCTGGAAATTATTCAAT95910.6885975398307477No Hit
ATATAGTAGCGCTGAGCTTTCATGGCCTTCTGCTATTTCAAATGCTTCAT95270.684002581792048No Hit
CATTATAGGTATTTCTTCACAAGAGCTGAATTTCCCATAGAGCTCTGCTT76740.5509641873278237No Hit
GCTTTAGCACTTCCATTACATCCTTTGCAATTCCTTCCCCATTCTTCTGA74220.532871540050444No Hit
GCCTTGGAATGGATAAAAAACAAAAGATGCTTAACTGATATACAGAAAGC70840.5086044179085617No Hit
GTTCATAGCTGAGACCATCTGCATTTCCCGTCTCACTCCTGGCACTGAGG66390.4766551002957286No Hit
ACTATATTGTCTCATGGTCATGTACCTGAATCCTGGAAATTATTCAATGC66230.47550636078605374No Hit
GTGTGAATGTGATGCTTGTTTTTCGCACAAAGCACAGAGTGTTCCTAGTT62060.4455673373151517No Hit
ATATTGTCTCATGGTCATGTACCTGAATCCTGGAAATTATTCAATGCAAG62060.4455673373151517No Hit
GTAAGAAATACAGTAAAAATTGAATTTAATGCGATTCTTCTACCTCCAAA60830.4367364023345258No Hit
CATTCACACAGAGCTCATAGCAGAGCAGCGAGATCCTCAGTGCCAGGAGT58080.41699244201198854No Hit
GCTATGAGCTCTGTGTGAATGTGATGCTTGTTTTTCGCACAAAGCACAGA57720.41440777811521995No Hit
GCTCATAGCAGAGCAGCGAGATCCTCAGTGCCAGGAGTGAGACGGGAAAT48690.34957579203794287No Hit
CTTCTGACTTGCCCCAAGGGATCTCAATACTCCAATGTTGCTTTGCAGTT46280.3322729031734647No Hit
GTCATGTACCTGAATCCTGGAAATTATTCAATGCAAGTAAAACTAGGAAC45900.3295446468379868No Hit
ATTACATCCTTTGCAATTCCTTCCCCATTCTTCTGACTTGCCCCAAGGGA43690.3136776823606023No Hit
GTCCTATTGTCCAAGCCATGAAATGGAGAGCTGATAAAATAAAAGAACAA43690.3136776823606023No Hit
CTGATATACAGAAAGCACTAATTGGTGCCTCTATCTGCTTTTTAAAACCC43640.3133187012638288No Hit
GCACTAATTGGTGCCTCTATCTGCTTTTTAAAACCCAAAGACCAGGAAAG43390.3115237957799618No Hit
AAATTACACTGTTGGTTCGGTGGGAAAGAATTTGACCTAGACTCTGCCTT42750.30692883774126223No Hit
GACCTAGACTCTGCCTTGGAATGGATAAAAAACAAAAGATGCTTAACTGA42110.3023338797025626No Hit
GTCTCATGGTCATGTACCTGAATCCTGGAAATTATTCAATGCAAGTAAAA41530.2981696989799911No Hit
GCACAGAGTGTTCCTAGTTTTACTTGCATTGAATAATTTCCAGGATTCAG41470.297738921663863No Hit
GCTATGAACACAGCAAAAACAATGAATGGAATGGGGAAAGGAGAAGACGT40250.2889797829025919No Hit
ATTCATCACAGAGCCCTTATCGGGAATGGGAACAACAGCAACAAAAAAGA34790.24977904713493598No Hit
AGCACGCACTTTCTTAAAATGTCGCTGTTTGGAGACACAATTGCCTACCT34460.24740977189623148No Hit
CTCCAATGTTGCTTTGCAGTTCTTCTGCCAGTTTTTGGACGTCTTCTCCT34210.24561486641236446No Hit
GAATAAAGGGGCCAAATAAAGAGACAATAAACAGAGAGGTATCAATTTTG33350.2394403915478619No Hit
ACTCTGTGCTTTGTGCGAAAAACAAGCATCACATTCACACAGAGCTCATA33260.23879422557366975No Hit
GTTCAAACATTATAGGTATTTCTTCACAAGAGCTGAATTTCCCATAGAGC31860.22874275486401435No Hit
GGTTCAAACATTATAGGTATTTCTTCACAAGAGCTGAATTTCCCATAGAG31250.22436318548337883No Hit
CCATTACATCCTTTGCAATTCCTTCCCCATTCTTCTGACTTGCCCCAAGG30850.22149133670919158No Hit
CTATTGTCCAAGCCATGAAATGGAGAGCTGATAAAATAAAAGAACAAATT30660.22012720854145262No Hit
CAATACTCCAATGTTGCTTTGCAGTTCTTCTGCCAGTTTTTGGACGTCTT30310.21761434086403877No Hit
GTCCAAGCCATGAAATGGAGAGCTGATAAAATAAAAGAACAAATTGAAAG29600.2125168092898564No Hit
AGCAGGCACTTTCTTAAAATGTCGCTGTTTGGAGACACAATTGCCTACCT29430.2112962735608268No Hit
TTCACACAGAGCTCATAGCAGAGCAGCGAGATCCTCAGTGCCAGGAGTGA29300.21036292270921597No Hit
TTCTTAAAATGTCGCTGTTTGGAGACACAATTGCCTACCTGCTTTCATTG29020.20835262856728493No Hit
TTGCAATTCCTTCCCCATTCTTCTGACTTGCCCCAAGGGATCTCAATACT28880.20734748149631937No Hit
GTTGTTCCCATTCCCGATAAGGGCTCTGTGATGAATCTTCTTTTTCTTTC27370.19650625237376249No Hit
ATGCTTAACTGATATACAGAAAGCACTAATTGGTGCCTCTATCTGCTTTT26090.1873163362963633No Hit
GAATAATTTCCAGGATTCAGGTACATGACCATGAGACAATATAGTAGCGC26090.1873163362963633No Hit
TAGTAGCGCTGAGCTTTCATGGCCTTCTGCTATTTCAAATGCTTCATGAA24730.17755205046412664No Hit
CTCCTGGCACTGAGGATCTCGCTGCTCTGCTATGAGCTCTGTGTGAATGT24600.1766186996125158No Hit
TTCATGGCCTTCTGCTATTTCAAATGCTTCATGAAAGCTCACACATTTTC24540.17618792229638772No Hit
CAATATAGTAGCGCTGAGCTTTCATGGCCTTCTGCTATTTCAAATGCTTC24530.17611612607703303No Hit
CCATGAGACAATATAGTAGCGCTGAGCTTTCATGGCCTTCTGCTATTTCA24460.17561355254155026No Hit
GTACATGACCATGAGACAATATAGTAGCGCTGAGCTTTCATGGCCTTCTG24350.17482379412864876No Hit
GATTCTGGCTGAGAGAAAAATGAGAAAATGTGTGAGCTTTCATGAAGCAT24350.17482379412864876No Hit
CTGTTGGTTCGGTGGGAAAGAATTTGACCTAGACTCTGCCTTGGAATGGA24340.1747519979092941No Hit
GCGCTACTATATTGTCTCATGGTCATGTACCTGAATCCTGGAAATTATTC23250.16692620999963384No Hit
AGTAGAAACAACGCACTTTTTCCAGTTTATTTGCTGACATTGATTACAAT23080.16570567427060426No Hit
AGACAATATAGTAGCGCTGAGCTTTCATGGCCTTCTGCTATTTCAAATGC22990.1650595082964121No Hit
AAATTATTCAATGCAAGTAAAACTAGGAACACTCTGTGCTTTGTGCGAAA22880.16426974988351062No Hit
CAATTGCCTACCTGCTTTCATTGACAGAAGATGGAGAAGGCAAAGCAGAA22740.1632646028125451No Hit
CTTCCATTACATCCTTTGCAATTCCTTCCCCATTCTTCTGACTTGCCCCA22720.16312101037383575No Hit
CCTTTCCCCATTCCATTCATTGTTTTTGCTGTGTTCATAGCTGAGACCAT22610.16233125196093423No Hit
GAGTGTTCCTAGTTTTACTTGCATTGAATAATTTCCAGGATTCAGGTACA22550.16190047464480617No Hit
TTCATGAAGCATTTGAAATAGCAGAAGGCCATGAAAGCTCAGCGCTACTA22520.1616850859867421No Hit
ATCTGGAATGGTTCAAACATTATAGGTATTTCTTCACAAGAGCTGAATTT22190.15931581074803763No Hit
TCTCAATACTCCAATGTTGCTTTGCAGTTCTTCTGCCAGTTTTTGGACGT21980.15780809014158934No Hit
CCCCAAGGGATCTCAATACTCCAATGTTGCTTTGCAGTTCTTCTGCCAGT21800.15651575819320507No Hit
GCCCTTATCGGGAATGGGAACAACAGCAACAAAAAAGAAGGGCCTGATTC21770.156300369535141No Hit
GCCCCAAGGGATCTCAATACTCCAATGTTGCTTTGCAGTTCTTCTGCCAG21460.1540746867351459No Hit
GTGCCAGGAGTGAGACGGGAAATGCAGATGGTCTCAGCTATGAACACAGC21170.15199259637386014No Hit
GTTTGGAGACACAATTGCCTACCTGCTTTCATTGACAGAAGATGGAGAAG21070.15127463418031334No Hit
ATTTAAATGTCCTATTGTCCAAGCCATGAAATGGAGAGCTGATAAAATAA20590.14782841565128862No Hit
ATTACTATGTGGTCACTCAATACTTCCATGTTGTTAGAGAGTACTTCCTT20470.14696686101903247No Hit
GAAATGGAGAGCTGATAAAATAAAAGAACAAATTGAAAGAATCTGGAATG20420.14660787992225904No Hit
CCCAAAGACCAGGAAAGAAAAAGAAGATTCATCACAGAGCCCTTATCGGG20290.1456745290706482No Hit
CTGTGTGAATGTGATGCTTGTTTTTCGCACAAAGCACAGAGTGTTCCTAG20240.14531554797387478No Hit
CCTATAATGTTTGAACCATTCCAGATTCTTTCAATTTGTTCTTTTATTTT19670.14122316347065794No Hit
TCCCCATTCCATTCATTGTTTTTGCTGTGTTCATAGCTGAGACCATCTGC19440.1395718504255003No Hit
CTTCACAAGAGCTGAATTTCCCATAGAGCTCTGCTTTAGCACTTCCATTA19230.138064129819052No Hit
CTCTGTGCTTTGTGCGAAAAACAAGCATCACATTCACACAGAGCTCATAG19130.1373461676255052No Hit
GTTTTTGCTGTGTTCATAGCTGAGACCATCTGCATTTCCCGTCTCACTCC19060.13684359409002242No Hit
TTATAGGTATTTCTTCACAAGAGCTGAATTTCCCATAGAGCTCTGCTTTA18980.13626922433518496No Hit
ACTAGGAACACTCTGTGCTTTGTGCGAAAAACAAGCATCACATTCACACA18700.13425893019325388No Hit
GCTGAGACCATCTGCATTTCCCGTCTCACTCCTGGCACTGAGGATCTCGC18290.13131528519971195No Hit
GTTGGTTCGGTGGGAAAGAATTTGACCTAGACTCTGCCTTGGAATGGATA18220.13081271166422917No Hit
TTGTTAGAGAGTACTTCCTTCATTGCTTCCTTAGCCTGAATTTCTTTTTG17990.1291613986190715No Hit
CCATAGAGCTCTGCTTTAGCACTTCCATTACATCCTTTGCAATTCCTTCC17990.1291613986190715No Hit
TAGCAGAGCAGCGAGATCCTCAGTGCCAGGAGTGAGACGGGAAATGCAGA17970.12901780618036215No Hit
GAAGATGGAGAAGGCAAAGCAGAATTAGCAGAAAAATTACACTGTTGGTT17880.12837164020617No Hit
CATTACATCCTTTGCAATTCCTTCCCCATTCTTCTGACTTGCCCCAAGGG17620.12650493850294833No Hit
TCACAGAGCCCTTATCGGGAATGGGAACAACAGCAACAAAAAAGAAGGGC17310.1242792557029532No Hit
CTTAAAATGTCGCTGTTTGGAGACACAATTGCCTACCTGCTTTCATTGAC17170.12327410863198765No Hit
GTTTATTGTCTCTTTATTTGGCCCCTTTATTCTTATTTTCATGTTTACTC16610.1192535203481255No Hit
CTATAGAACATGCTTGTAAATTCAAACATTCCAGTTTCGTTACAGTAACT16290.11695604132877571No Hit
GGCCATGAAAGCTCAGCGCTACTATATTGTCTCATGGTCATGTACCTGAA15980.1147303585287806No Hit
GTGTTCCTAGTTTTACTTGCATTGAATAATTTCCAGGATTCAGGTACATG15950.11451496987071655No Hit
GGGGAAAGGAGAAGACGTCCAAAAACTGGCAGAAGAACTGCAAAGCAACA15920.1142995812126525No Hit
TCTATCTGCTTTTTAAAACCCAAAGACCAGGAAAGAAAAAGAAGATTCAT15890.11408419255458846No Hit
GGAGAAGGCAAAGCAGAATTAGCAGAAAAATTACACTGTTGGTTCGGTGG15820.11358161901910568No Hit
GTATTTACACAGTCTCTCCAAAGTTTCCATAGGAAATTTTCGTTAGCTTT15690.11264826816749483No Hit
GCTCTGCTATGAGCTCTGTGTGAATGTGATGCTTGTTTTTCGCACAAAGC15400.11056617780620909No Hit
CAATTACTATGTGGTCACTCAATACTTCCATGTTGTTAGAGAGTACTTCC15360.11027899292879037No Hit
CTTATCGGGAATGGGAACAACAGCAACAAAAAAGAAGGGCCTGATTCTGG15350.11020719670943567No Hit
CTAGGAACACTCTGTGCTTTGTGCGAAAAACAAGCATCACATTCACACAG15240.10941743829653418No Hit
AATTGGTGCCTCTATCTGCTTTTTAAAACCCAAAGACCAGGAAAGAAAAA15160.10884306854169673No Hit
GTTCCTAGTTTTACTTGCATTGAATAATTTCCAGGATTCAGGTACATGAC15100.10841229122556864No Hit
GAATTTGACCTAGACTCTGCCTTGGAATGGATAAAAAACAAAAGATGCTT14890.10690457061912034No Hit
GACAATAAACAGAGAGGTATCAATTTTGAGACACAGTTACCAAAAAGAAA14830.10647379330299225No Hit
CTGTTGTTCCCATTCCCGATAAGGGCTCTGTGATGAATCTTCTTTTTCTT14710.10561223867073609No Hit
AATCTGGAATGGTTCAAACATTATAGGTATTTCTTCACAAGAGCTGAATT14460.10381733318686903No Hit
GGTCTCAGCTATGAACACAGCAAAAACAATGAATGGAATGGGGAAAGGAG14310.10274038989654882No Hit
AAGAAATACAGTAAAAATTGAATTTAATGCGATTCTTCTACCTCCAAAAC14290.10259679745783945No Hit
CCTTTATTCTTATTTTCATGTTTACTCTTCTTTTTATTTGATTTAAATGT14190.10187883526429264No Hit
GTCTTCTCCTTTCCCCATTCCATTCATTGTTTTTGCTGTGTTCATAGCTG14100.10123266929010052No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTGTAC100.0070861895145.0341
AGTCACT100.0070892326145.013185
GTACACT100.0070892326145.013184
TTAGTCA100.0070892326145.013183
TAGTCAC100.0070892326145.013184
CGAGACC100.007090755145.00276145
CACTGGC100.0070915157144.997568
AACACGT256.462853E-6116.0105446
GAGCAGA1400.0108.775511
AGCACGC4700.0103.3753051
CACGCAC5000.097.158833
ACACGTG301.599815E-596.665047
GCACGCA5050.096.196862
GAAACAC850.093.832054
AGTTATA502.267916E-887.0079042
CACGTGC353.4380384E-582.855758
AAACACG1000.079.757255
AACAACG4250.078.469277
TAGAAAC4300.077.565193
GAAGCGG851.8189894E-1276.782711
Produced by FastQC (version 0.11.5)