FastQCFastQC Report
Tue 2 Jul 2019
HWC5JBCX2_l01_n01_SM2_9932_1_P1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWC5JBCX2_l01_n01_SM2_9932_1_P1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1693059
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATATACAGAAAGCACTAATTGGTGCCTCTATCTGCTTTTTAAAACCCAA93260.5508372714713428No Hit
ATATAGTAGCGCTGAGCTTTCATGGCCTTCTGCTATTTCAAATGCTTCAT74920.4425126354131782No Hit
CTACTATATTGTCTCATGGTCATGTACCTGAATCCTGGAAATTATTCAAT71350.42142654213468045No Hit
GTAAGAAATACAGTAAAAATTGAATTTAATGCGATTCTTCTACCTCCAAA64930.38350701304561746No Hit
GCCTTGGAATGGATAAAAAACAAAAGATGCTTAACTGATATACAGAAAGC60380.35663258043576745No Hit
CATTATAGGTATTTCTTCACAAGAGCTGAATTTCCCATAGAGCTCTGCTT57270.3382634627617821No Hit
GTGTGAATGTGATGCTTGTTTTTCGCACAAAGCACAGAGTGTTCCTAGTT52670.31109370671665904No Hit
GCTTTAGCACTTCCATTACATCCTTTGCAATTCCTTCCCCATTCTTCTGA52490.310030542349676No Hit
ACTATATTGTCTCATGGTCATGTACCTGAATCCTGGAAATTATTCAATGC50260.29685911713649676No Hit
CATTCACACAGAGCTCATAGCAGAGCAGCGAGATCCTCAGTGCCAGGAGT49140.2902438721863798No Hit
GTTCATAGCTGAGACCATCTGCATTTCCCGTCTCACTCCTGGCACTGAGG46280.27335137168875984No Hit
ATATTGTCTCATGGTCATGTACCTGAATCCTGGAAATTATTCAATGCAAG46190.2728197895052683No Hit
GCTATGAGCTCTGTGTGAATGTGATGCTTGTTTTTCGCACAAAGCACAGA44960.26555483299755056No Hit
CTATAGAACATGCTTGTAAATTCAAACATTCCAGTTTCGTTACAGTAACT40360.23838507695242753No Hit
GTCCTATTGTCCAAGCCATGAAATGGAGAGCTGATAAAATAAAAGAACAA39300.23212422012463832No Hit
GCTCATAGCAGAGCAGCGAGATCCTCAGTGCCAGGAGTGAGACGGGAAAT37560.22184696457713524No Hit
CTGATATACAGAAAGCACTAATTGGTGCCTCTATCTGCTTTTTAAAACCC37460.2212563177065891No Hit
GCACTAATTGGTGCCTCTATCTGCTTTTTAAAACCCAAAGACCAGGAAAG37380.22078380021015215No Hit
CTTCTGACTTGCCCCAAGGGATCTCAATACTCCAATGTTGCTTTGCAGTT36830.21753524242214833No Hit
GCTATGAACACAGCAAAAACAATGAATGGAATGGGGAAAGGAGAAGACGT36330.21458200806941755No Hit
GTCATGTACCTGAATCCTGGAAATTATTCAATGCAAGTAAAACTAGGAAC36120.21334164964127061No Hit
GCACAGAGTGTTCCTAGTTTTACTTGCATTGAATAATTTCCAGGATTCAG35840.2116878384037414No Hit
ATTACATCCTTTGCAATTCCTTCCCCATTCTTCTGACTTGCCCCAAGGGA35520.20979776841799372No Hit
GACCTAGACTCTGCCTTGGAATGGATAAAAAACAAAAGATGCTTAACTGA34860.20589949907238908No Hit
AAATTACACTGTTGGTTCGGTGGGAAAGAATTTGACCTAGACTCTGCCTT34190.20194216503972984No Hit
GTCTCATGGTCATGTACCTGAATCCTGGAAATTATTCAATGCAAGTAAAA31490.1859946995349837No Hit
GAATAAAGGGGCCAAATAAAGAGACAATAAACAGAGAGGTATCAATTTTG30860.18227362425054294No Hit
ATTCATCACAGAGCCCTTATCGGGAATGGGAACAACAGCAACAAAAAAGA29520.17435895618522448No Hit
GTCCAAGCCATGAAATGGAGAGCTGATAAAATAAAAGAACAAATTGAAAG28490.16827529341859912No Hit
CTCCAATGTTGCTTTGCAGTTCTTCTGCCAGTTTTTGGACGTCTTCTCCT27630.16319573033190218No Hit
CTATTGTCCAAGCCATGAAATGGAGAGCTGATAAAATAAAAGAACAAATT27270.16106940159793603No Hit
AGTAGAAACAACGCACTTTTTCCAGTTTATTTGCTGACATTGATTACAAT26640.15734832631349527No Hit
ACTCTGTGCTTTGTGCGAAAAACAAGCATCACATTCACACAGAGCTCATA26460.1562851619465122No Hit
GTTCAAACATTATAGGTATTTCTTCACAAGAGCTGAATTTCCCATAGAGC25480.1504968226151599No Hit
GTATAGATCTGTTCCTTTTGGTTCTCCAGCTATGAGTTCCAGACACTGCA25200.14884301137763067No Hit
TTCTTAAAATGTCGCTGTTTGGAGACACAATTGCCTACCTGCTTTCATTG25150.1485476879423576No Hit
TACCTATACAGGCAGCAATTTCAACAACATTCCCATACACCGGTGTTCCC24700.14588977702489989No Hit
CCCAAGCAGCCACTCTACACTTGTTAGGGATTTTAGGCTCTTCTTGGCAC24660.14565351827668144No Hit
AGCACGCACTTTCTTAAAATGTCGCTGTTTGGAGACACAATTGCCTACCT24570.1451219360931899No Hit
TTCACACAGAGCTCATAGCAGAGCAGCGAGATCCTCAGTGCCAGGAGTGA24510.1447675479708622No Hit
GTGTACAATGATAGATCCAACAAATGGGCCATTACCTGAAGACAATGAGC24410.14417690110031606No Hit
GGTTCAAACATTATAGGTATTTCTTCACAAGAGCTGAATTTCCCATAGAG24150.14264121923689604No Hit
CAATACTCCAATGTTGCTTTGCAGTTCTTCTGCCAGTTTTTGGACGTCTT23820.14069208456409377No Hit
GTTCTATAGAGATGGATTTGTATCTAACTTTGCAATGGAAATTCCTTCAT22750.1343721630492499No Hit
CCATTACATCCTTTGCAATTCCTTCCCCATTCTTCTGACTTGCCCCAAGG22320.13183238150590146No Hit
TTCATGGCCTTCTGCTATTTCAAATGCTTCATGAAAGCTCACACATTTTC22140.13076921713891837No Hit
CCCATACACCGGTGTTCCCCCTTATTCCCATGGAACGGGAACAGGCTACA21990.12988324683309913No Hit
ATGCTTAACTGATATACAGAAAGCACTAATTGGTGCCTCTATCTGCTTTT21870.12917447058844375No Hit
GTTTATTGTCTCTTTATTTGGCCCCTTTATTCTTATTTTCATGTTTACTC21120.12474461905934761No Hit
GAATAATTTCCAGGATTCAGGTACATGACCATGAGACAATATAGTAGCGC20860.12320893719592761No Hit
CTGTTGGTTCGGTGGGAAAGAATTTGACCTAGACTCTGCCTTGGAATGGA20550.12137793189723455No Hit
ATTACTATGTGGTCACTCAATACTTCCATGTTGTTAGAGAGTACTTCCTT20390.12043289690436068No Hit
CTGTTAAAACAGGCCTCAAAAAGATTGCAACATTTAGCGTAGCATTGTTC20300.11990131472086915No Hit
GCCCTTATCGGGAATGGGAACAACAGCAACAAAAAAGAAGGGCCTGATTC20270.1197241206597053No Hit
GTGCCAGGAGTGAGACGGGAAATGCAGATGGTCTCAGCTATGAACACAGC19950.11783405067395761No Hit
TCTGCATACTGTCCAATCGAAAGTCTGTCTCCCCTGGGTTAATTTGTCTA19830.11712527442930222No Hit
TAGTAGCGCTGAGCTTTCATGGCCTTCTGCTATTTCAAATGCTTCATGAA19800.11694808036813839No Hit
CTCCTGGCACTGAGGATCTCGCTGCTCTGCTATGAGCTCTGTGTGAATGT19690.11629836881053761No Hit
TTGTTAGAGAGTACTTCCTTCATTGCTTCCTTAGCCTGAATTTCTTTTTG19630.11594398068820994No Hit
CCATGAGACAATATAGTAGCGCTGAGCTTTCATGGCCTTCTGCTATTTCA19630.11594398068820994No Hit
TTGCAATTCCTTCCCCATTCTTCTGACTTGCCCCAAGGGATCTCAATACT19540.11541239850471838No Hit
GTATTTAGTATAGATCTGTTCCTTTTGGTTCTCCAGCTATGAGTTCCAGA19490.1151170750694453No Hit
GTACATGACCATGAGACAATATAGTAGCGCTGAGCTTTCATGGCCTTCTG19420.11470362226006299No Hit
ATCCAATGATGACCAATAACCCCATGGACATCTTCGGAGCTTATGTGTAC19330.11417204007657146No Hit
ATTTAAATGTCCTATTGTCCAAGCCATGAAATGGAGAGCTGATAAAATAA19040.11245916415198762No Hit
GTTAAAACAGGCCTCAAAAAGATTGCAACATTTAGCGTAGCATTGTTCTT19000.11222290540376917No Hit
GCCCCAAGGGATCTCAATACTCCAATGTTGCTTTGCAGTTCTTCTGCCAG18880.11151412915911378No Hit
CTTCCATTACATCCTTTGCAATTCCTTCCCCATTCTTCTGACTTGCCCCA18650.11015564135685761No Hit
CAATATAGTAGCGCTGAGCTTTCATGGCCTTCTGCTATTTCAAATGCTTC18630.11003751198274839No Hit
ATCTGGAATGGTTCAAACATTATAGGTATTTCTTCACAAGAGCTGAATTT18590.10980125323452994No Hit
TTCATGAAGCATTTGAAATAGCAGAAGGCCATGAAAGCTCAGCGCTACTA18440.1089152829287107No Hit
GCGCTACTATATTGTCTCATGGTCATGTACCTGAATCCTGGAAATTATTC18390.10861995949343763No Hit
GAAATGGAGAGCTGATAAAATAAAAGAACAAATTGAAAGAATCTGGAATG18290.10802931262289146No Hit
CAATTGCCTACCTGCTTTCATTGACAGAAGATGGAGAAGGCAAAGCAGAA18120.10702521294296301No Hit
GTATGCAGAAACCAGCCTGCTGCAACGGCACTAAACACAACAATAACCTC17920.1058439192018707No Hit
CCTATAATGTTTGAACCATTCCAGATTCTTTCAATTTGTTCTTTTATTTT17900.10572578982776147No Hit
TCTCAATACTCCAATGTTGCTTTGCAGTTCTTCTGCCAGTTTTTGGACGT17760.10489888420899686No Hit
AGACAATATAGTAGCGCTGAGCTTTCATGGCCTTCTGCTATTTCAAATGC17690.10448543139961455No Hit
GTTTGGAGACACAATTGCCTACCTGCTTTCATTGACAGAAGATGGAGAAG17620.10407197859023225No Hit
GATCTATACTAAATACTGATCAGAGGAACATGATTCTTGAAGAACAATGC17430.10294974953619454No Hit
GATTCTGGCTGAGAGAAAAATGAGAAAATGTGTGAGCTTTCATGAAGCAT17350.10247723203975763No Hit
CTGTGTGAATGTGATGCTTGTTTTTCGCACAAAGCACAGAGTGTTCCTAG17210.10165032642099302No Hit
GTCTAGATATTGTTTGTCCAAGTCTAAAGTCAATTCCCTTTTCAACATCT17060.10076435611517379No Hit
AAATTATTCAATGCAAGTAAAACTAGGAACACTCTGTGCTTTGTGCGAAA16980.10029183861873685No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCACTG151.2297634E-4145.011585
CGGAGCG100.007089601145.011585
GGAGCGT100.0070902267145.00736
CGGCATA100.0070914794144.99873145
ATAAGCC203.8666598E-4108.74905145
AAACACG2950.0100.770755
GAAACAC2900.0100.007994
AGCACGC3700.099.969941
AACACGA1750.099.433576
GCACGCA3750.098.610782
AACACGT1250.098.604966
ACACGAG1850.094.053247
ACGTGCC900.088.610349
GAGCAGA1500.087.032651
ACGAGCC1600.086.0929959
ACACGTG1450.084.999257
CACGAGC2050.084.8773048
CACGCAC4750.080.903593
CACGTGC1650.074.696318
ACAACGC5950.074.327088
Produced by FastQC (version 0.11.5)