FastQCFastQC Report
Tue 2 Jul 2019
HWC5JBCX2_l01_n01_SM2_9841_P1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWC5JBCX2_l01_n01_SM2_9841_P1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1580475
Sequences flagged as poor quality0
Sequence length151
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAAGAAATACAGTAAAAATTGAATTTAATGCGATTCTTCTACCTCCAAA115980.7338300194561762No Hit
CTATAGAACATGCTTGTAAATTCAAACATTCCAGTTTCGTTACAGTAACT79060.5002293614261535No Hit
GAATAAAGGGGCCAAATAAAGAGACAATAAACAGAGAGGTATCAATTTTG67500.42708679352726237No Hit
GTTCTATAGAGATGGATTTGTATCTAACTTTGCAATGGAAATTCCTTCAT46470.2940255302994353No Hit
AGTAGAAACAACGCACTTTTTCCAGTTTATTTGCTGACATTGATTACAAT44980.2845979847830557No Hit
TTCTAATGGTATGCTGAACAGATCAGGACAAGGTATTTGAGCCTTTAGTC35890.2270836299213844No Hit
GTATAGATCTGTTCCTTTTGGTTCTCCAGCTATGAGTTCCAGACACTGCA31510.19937044243028204No Hit
GATCAGGACAAGGTATTTGAGCCTTTAGTCTTTTTGTTTTACTTGTTATC31500.19930717031272244No Hit
CCTATAATGTTTGAACCATTCCAGATTCTTTCAATTTGTTCTTTTATTTT30730.19443521726063367No Hit
GTATAGCACCTGTCTTGTTTTCCAACAAAATAGCCAGATTAGGGAAAGGA29590.1872221958588399No Hit
GACAATAAACAGAGAGGTATCAATTTTGAGACACAGTTACCAAAAAGAAA29460.18639965833056518No Hit
TTATTATTGTCATTCCTATTGCCATATCTGCTGATTCATTTACTCCAGCA29200.18475458327401573No Hit
ATTACTATGTGGTCACTCAATACTTCCATGTTGTTAGAGAGTACTTCCTT29180.18462803903889655No Hit
AAGAAATACAGTAAAAATTGAATTTAATGCGATTCTTCTACCTCCAAAAC28250.17874373210585426No Hit
GTGTACAATGATAGATCCAACAAATGGGCCATTACCTGAAGACAATGAGC27460.1737452348186463No Hit
CCATTAGAAAGATATAATGAAGAAACAAGGGCGAAATTAAAAAGGCTGAA27130.1716572549391797No Hit
GTCCTGATCTGTTCAGCATACCATTAGAAAGATATAATGAAGAAACAAGG26610.16836710482608078No Hit
TACCTATACAGGCAGCAATTTCAACAACATTCCCATACACCGGTGTTCCC26370.1668485740046505No Hit
ATACTAAATGGAATGAATGCTTAAATCCACGAATCTTTTTGGCTATGACT26220.16589949224125658No Hit
GTTTAATATGCTATCTACCGTGTTGGGAGTAGCAGCACTAGGCATCAAAA26190.1657096758885778No Hit
ATCCAATGATGACCAATAACCCCATGGACATCTTCGGAGCTTATGTGTAC25940.16412787294958794No Hit
GTGCTATACTACAAAAATCCCGGAACCAAATTGGGCTGTCTCTGGTTATT25650.1622929815403597No Hit
CCCATACACCGGTGTTCCCCCTTATTCCCATGGAACGGGAACAGGCTACA25510.16140717189452536No Hit
TCTGCATACTGTCCAATCGAAAGTCTGTCTCCCCTGGGTTAATTTGTCTA25310.1601417295433335No Hit
CAATAAACAGAGAGGTATCAATTTTGAGACACAGTTACCAAAAAGAAATT24390.15432069472785082No Hit
ACAATAAACAGAGAGGTATCAATTTTGAGACACAGTTACCAAAAAGAAAT23270.14723421756117622No Hit
GTTAAAACAGGCCTCAAAAAGATTGCAACATTTAGCGTAGCATTGTTCTT22820.14438697227099448No Hit
GTATTAGTAGTTGAAAACTTGGCTAAGAACATCTGTGAAAATCTAGAACA22710.14369097897783895No Hit
CAATTACTATGTGGTCACTCAATACTTCCATGTTGTTAGAGAGTACTTCC22700.14362770686027934No Hit
GATCTATACTAAATACTGATCAGAGGAACATGATTCTTGAAGAACAATGC22680.14350116262516016No Hit
GTATTTAGTATAGATCTGTTCCTTTTGGTTCTCCAGCTATGAGTTCCAGA22570.14280516933200463No Hit
CTGTTAAAACAGGCCTCAAAAAGATTGCAACATTTAGCGTAGCATTGTTC22540.14261535297932584No Hit
ATTTAGTATTGTCTCCTGTTACTGTCATGCTGATCCCTCCTGGTGGGCAG22260.1408437336876572No Hit
GATTATAGGTACACATACAAATGCCACAGAGGAGATTCCAAAGTGGAAGG22250.1407804615700976No Hit
GAGTAAACATGAAAATAAGAATAAAGGGGCCAAATAAAGAGACAATAAAC22000.13919865863110775No Hit
CTTATGTATTGTTCCGTTCCCGATTAATATTTCTTCATCATTTTTGAATC21920.13869248169063098No Hit
ATGCTGAACAGATCAGGACAAGGTATTTGAGCCTTTAGTCTTTTTGTTTT21500.13603505275312802No Hit
TTGTTAGAGAGTACTTCCTTCATTGCTTCCTTAGCCTGAATTTCTTTTTG21360.1351492431072937No Hit
GTTAGTAGCAGATGGTGGGCCCAACATTTACAATTTGAGAAACTTACATA21150.1338205286385422No Hit
CTCCAAAACTGTTTCACCCATTTTTATTATCTCTTCAGCAGAAAGCCCCT21050.13318780746294626No Hit
GCTTAAATGCATAGTAAGAAATACAGTAAAAATTGAATTTAATGCGATTC20670.13078346699568166No Hit
CATGTATCCAATGATGACCAATAACCCCATGGACATCTTCGGAGCTTATG20550.13002420158496653No Hit
CAATAGGACATTTAAATCAAATAAAAAGAAGAGTAAACATGAAAATAAGA20390.12901184770401303No Hit
GTCTCTGGTTATTCTTTCAGTCATAGCCAAAAAGATTCGTGGATTTAAGC20300.12844239864597667No Hit
AATAAAGAGACAATAAACAGAGAGGTATCAATTTTGAGACACAGTTACCA20240.12806276594061913No Hit
GTATGCAGAAACCAGCCTGCTGCAACGGCACTAAACACAACAATAACCTC20210.12787294958794035No Hit
ACCTAATGGACCCTGAATACAAAGGGCGGTTACTTCACCCTCAAAATCCC20140.12743004476502318No Hit
GGCTTGGACAATAGGACATTTAAATCAAATAAAAAGAAGAGTAAACATGA19460.12312754077097075No Hit
GTTTTAACAGTGCATCATACAGGAAACCAGTAGGGCAACATAGCATGCTT19400.12274790806561318No Hit
CTTCAGCAGAAAGCCCCTCAATTACTATGTGGTCACTCAATACTTCCATG19390.12268463594805361No Hit
GTTCAGCATACCATTAGAAAGATATAATGAAGAAACAAGGGCGAAATTAA19340.12236827536025563No Hit
GTTCTTTATTATTGTCATTCCTATTGCCATATCTGCTGATTCATTTACTC19290.12205191477245765No Hit
GAAATACAGTAAAAATTGAATTTAATGCGATTCTTCTACCTCCAAAACTG19270.12192537053733844No Hit
ACTAAATACTGATCAGAGGAACATGATTCTTGAAGAACAATGCTACGCTA19160.12122937724418292No Hit
GATTACAATTTGCTTAAATGCATAGTAAGAAATACAGTAAAAATTGAATT19110.12091301665638494No Hit
CCTATACAGGCAGCAATTTCAACAACATTCCCATACACCGGTGTTCCCCC18810.11901485312959713No Hit
ATGCTACACTGCTTACAAGGTGAAAATTGGAGGCAAATATATCACCCAGG18800.11895158101203752No Hit
TTCTTAATCTATGGGCCATAGCCTCAAGCATGCTATGTTGCCCTACTGGT18800.11895158101203752No Hit
GGACAATAGGACATTTAAATCAAATAAAAAGAAGAGTAAACATGAAAATA18650.11800249924864362No Hit
GTTAGATACAAATCCATCTCTATAGAACATGCTTGTAAATTCAAACATTC18270.11559815878137902No Hit
ATATTAAACATTCCCATCATCATCCCAGGCGACAAAGATGCTGTTCCTTC17920.1133836346667932No Hit
CATCAAAAGAGCATTGTCATTAAACACAATGACAAAAGATGCTGAAAGGG17750.1123080086682801No Hit
TCTTTTTAGTTTGCCCCTTTCAGCATCTTTTGTCATTGTGTTTAATGACA17420.1102200287888135No Hit
GTTCATAGCTGATTATAGGTACACATACAAATGCCACAGAGGAGATTCCA17080.10806877679178728No Hit
GTATGGAAGGGATAAATGATTTTTACCGAACATGTAAATTATTGGGAATA17050.10787896043910851No Hit
TAATAATACTGCTGCTCTTTTCAACCACCATGCGTTCTGGTGGTAGGCTG17030.1077524162039893No Hit
CACTTACACACACGCGTAACACAGGTTTTAGACGGATACAAGCGCCTTTG16890.10686660655815497No Hit
CTCCATTTCATGGCTTGGACAATAGGACATTTAAATCAAATAAAAAGAAG16860.1066767902054762No Hit
TGTTTATTCCCAATAATTTACATGTTCGGTAAAAATCATTTATCCCTTCC16730.10585425267720147No Hit
GTATCTAACTTTGCAATGGAAATTCCTTCATTTGGAGTTGCTGGAGTAAA16480.1042724497382116No Hit
GTATTGAAGTACAACCTAATGGACCCTGAATACAAAGGGCGGTTACTTCA16480.1042724497382116No Hit
CTTATGGGATGGACTGCAATCTTCTGATGATTTTGCTTTGTTTGTTAATG16410.10382954491529445No Hit
CTTACACACACGCGTAACACAGGTTTTAGACGGATACAAGCGCCTTTGAA16330.1033233679748177No Hit
ACAGAGAGGTATCAATTTTGAGACACAGTTACCAAAAAGAAATTCAGGCT16210.10256410256410256No Hit
GTATTGTTCCGTTCCCGATTAATATTTCTTCATCATTTTTGAATCCTCTT16180.10237428621142379No Hit
ACCAGAGACAGCCCAATTTGGTTCCGGGATTTTTGTAGTATAGCACCTGT16170.10231101409386419No Hit
GTAAAAATTGAATTTAATGCGATTCTTCTACCTCCAAAACTGTTTCACCC15860.10034957844951677No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGG100.007088265145.020423
ACGTGCC850.0110.880429
GAGCAGA1250.0110.2329561
AACACGT3400.0106.62596
ACACGTG3450.0105.070637
CACGTGC3800.095.393088
AAACACG6700.090.908325
ACGTGCG2200.088.975729
GAAACAC6950.087.638234
GAGAAAT259.3825173E-487.012243
ACAACGC9850.079.4918
GTAGGCA554.391768E-879.102043
TAGGCAA554.391768E-879.102044
CAACGCA10250.076.388919
ACACGAG3600.074.512597
GGAGCCT809.822543E-1172.505626
AACAACG11150.070.222997
ACGAGCC1900.068.6830149
ACGAGCG1500.067.665489
TGTAGGC651.3972203E-766.9324952
Produced by FastQC (version 0.11.5)