FastQCFastQC Report
Thu 11 Jul 2019
HWC5JBCX2_l01_n01_SM2_9841_P1_DM2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWC5JBCX2_l01_n01_SM2_9841_P1_DM2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1586487
Sequences flagged as poor quality0
Sequence length151
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAAGAAATACAGTAAAAATTGAATTTAATGCGATTCTTCTACCTCCAAA116410.7337595580676047No Hit
CTATAGAACATGCTTGTAAATTCAAACATTCCAGTTTCGTTACAGTAACT79430.5006659367520818No Hit
GAATAAAGGGGCCAAATAAAGAGACAATAAACAGAGAGGTATCAATTTTG67680.42660292835680347No Hit
GTTCTATAGAGATGGATTTGTATCTAACTTTGCAATGGAAATTCCTTCAT46730.2945501601967113No Hit
AGTAGAAACAACGCACTTTTTCCAGTTTATTTGCTGACATTGATTACAAT45260.2852844051038553No Hit
TTCTAATGGTATGCTGAACAGATCAGGACAAGGTATTTGAGCCTTTAGTC35960.22666432186333707No Hit
GATCAGGACAAGGTATTTGAGCCTTTAGTCTTTTTGTTTTACTTGTTATC31600.19918221832262098No Hit
GTATAGATCTGTTCCTTTTGGTTCTCCAGCTATGAGTTCCAGACACTGCA31590.19911918597505054No Hit
CCTATAATGTTTGAACCATTCCAGATTCTTTCAATTTGTTCTTTTATTTT30860.1945178246024077No Hit
GTATAGCACCTGTCTTGTTTTCCAACAAAATAGCCAGATTAGGGAAAGGA29720.18733213697937645No Hit
GACAATAAACAGAGAGGTATCAATTTTGAGACACAGTTACCAAAAAGAAA29530.1861345223755379No Hit
TTATTATTGTCATTCCTATTGCCATATCTGCTGATTCATTTACTCCAGCA29290.18462174603384712No Hit
ATTACTATGTGGTCACTCAATACTTCCATGTTGTTAGAGAGTACTTCCTT29280.18455871368627666No Hit
AAGAAATACAGTAAAAATTGAATTTAATGCGATTCTTCTACCTCCAAAAC28350.17869670536222484No Hit
GTGTACAATGATAGATCCAACAAATGGGCCATTACCTGAAGACAATGAGC27570.17378018225172978No Hit
CCATTAGAAAGATATAATGAAGAAACAAGGGCGAAATTAAAAAGGCTGAA27170.17125888834891176No Hit
GTCCTGATCTGTTCAGCATACCATTAGAAAGATATAATGAAGAAACAAGG26660.16804423862281884No Hit
TACCTATACAGGCAGCAATTTCAACAACATTCCCATACACCGGTGTTCCC26500.16703572106169165No Hit
ATACTAAATGGAATGAATGCTTAAATCCACGAATCTTTTTGGCTATGACT26330.165964171152994No Hit
GTTTAATATGCTATCTACCGTGTTGGGAGTAGCAGCACTAGGCATCAAAA26260.16552294472000087No Hit
ATCCAATGATGACCAATAACCCCATGGACATCTTCGGAGCTTATGTGTAC26070.16432533011616232No Hit
GTGCTATACTACAAAAATCCCGGAACCAAATTGGGCTGTCTCTGGTTATT25760.16237132734147838No Hit
CCCATACACCGGTGTTCCCCCTTATTCCCATGGAACGGGAACAGGCTACA25560.16111068039006937No Hit
TCTGCATACTGTCCAATCGAAAGTCTGTCTCCCCTGGGTTAATTTGTCTA25400.16010216282894218No Hit
CAATAAACAGAGAGGTATCAATTTTGAGACACAGTTACCAAAAAGAAATT24470.15424015450489037No Hit
ACAATAAACAGAGAGGTATCAATTTTGAGACACAGTTACCAAAAAGAAAT23330.1470544668818591No Hit
GTTAAAACAGGCCTCAAAAAGATTGCAACATTTAGCGTAGCATTGTTCTT22900.14434407593632975No Hit
GTATTAGTAGTTGAAAACTTGGCTAAGAACATCTGTGAAAATCTAGAACA22790.14365072011305483No Hit
CAATTACTATGTGGTCACTCAATACTTCCATGTTGTTAGAGAGTACTTCC22760.14346162307034346No Hit
GATCTATACTAAATACTGATCAGAGGAACATGATTCTTGAAGAACAATGC22740.14333555837520257No Hit
CTGTTAAAACAGGCCTCAAAAAGATTGCAACATTTAGCGTAGCATTGTTC22660.14283129959463897No Hit
GTATTTAGTATAGATCTGTTCCTTTTGGTTCTCCAGCTATGAGTTCCAGA22660.14283129959463897No Hit
ATTTAGTATTGTCTCCTGTTACTGTCATGCTGATCCCTCCTGGTGGGCAG22330.14075123212481414No Hit
GATTATAGGTACACATACAAATGCCACAGAGGAGATTCCAAAGTGGAAGG22320.14068819977724367No Hit
GAGTAAACATGAAAATAAGAATAAAGGGGCCAAATAAAGAGACAATAAAC22050.13898632639284153No Hit
CTTATGTATTGTTCCGTTCCCGATTAATATTTCTTCATCATTTTTGAATC21970.13848206761227794No Hit
ATGCTGAACAGATCAGGACAAGGTATTTGAGCCTTTAGTCTTTTTGTTTT21570.13596077370945997No Hit
TTGTTAGAGAGTACTTCCTTCATTGCTTCCTTAGCCTGAATTTCTTTTTG21400.13488922380076232No Hit
GTTAGTAGCAGATGGTGGGCCCAACATTTACAATTTGAGAAACTTACATA21230.13381767389206467No Hit
CTCCAAAACTGTTTCACCCATTTTTATTATCTCTTCAGCAGAAAGCCCCT21180.1335025121542124No Hit
GCTTAAATGCATAGTAAGAAATACAGTAAAAATTGAATTTAATGCGATTC20750.13079212120868308No Hit
CATGTATCCAATGATGACCAATAACCCCATGGACATCTTCGGAGCTTATG20620.12997270069026723No Hit
CAATAGGACATTTAAATCAAATAAAAAGAAGAGTAAACATGAAAATAAGA20430.1287750860864287No Hit
GTCTCTGGTTATTCTTTCAGTCATAGCCAAAAAGATTCGTGGATTTAAGC20350.12827082730586511No Hit
GTATGCAGAAACCAGCCTGCTGCAACGGCACTAAACACAACAATAACCTC20320.12808173026315375No Hit
AATAAAGAGACAATAAACAGAGAGGTATCAATTTTGAGACACAGTTACCA20310.1280186979155833No Hit
ACCTAATGGACCCTGAATACAAAGGGCGGTTACTTCACCCTCAAAATCCC20250.1276405038301606No Hit
GGCTTGGACAATAGGACATTTAAATCAAATAAAAAGAAGAGTAAACATGA19510.12297611010994731No Hit
GTTTTAACAGTGCATCATACAGGAAACCAGTAGGGCAACATAGCATGCTT19480.12278701306723597No Hit
CTTCAGCAGAAAGCCCCTCAATTACTATGTGGTCACTCAATACTTCCATG19440.12253488367695417No Hit
GTTCTTTATTATTGTCATTCCTATTGCCATATCTGCTGATTCATTTACTC19380.12215668959153148No Hit
GTTCAGCATACCATTAGAAAGATATAATGAAGAAACAAGGGCGAAATTAA19370.12209365724396101No Hit
GAAATACAGTAAAAATTGAATTTAATGCGATTCTTCTACCTCCAAAACTG19330.12184152785367922No Hit
GATTACAATTTGCTTAAATGCATAGTAAGAAATACAGTAAAAATTGAATT19200.12102210733526339No Hit
ACTAAATACTGATCAGAGGAACATGATTCTTGAAGAACAATGCTACGCTA19200.12102210733526339No Hit
CCTATACAGGCAGCAATTTCAACAACATTCCCATACACCGGTGTTCCCCC18930.11932023395086123No Hit
TTCTTAATCTATGGGCCATAGCCTCAAGCATGCTATGTTGCCCTACTGGT18870.11894203986543854No Hit
ATGCTACACTGCTTACAAGGTGAAAATTGGAGGCAAATATATCACCCAGG18830.11868991047515674No Hit
GGACAATAGGACATTTAAATCAAATAAAAAGAAGAGTAAACATGAAAATA18730.11805958699945224No Hit
GTTAGATACAAATCCATCTCTATAGAACATGCTTGTAAATTCAAACATTC18290.11528616370635246No Hit
ATATTAAACATTCCCATCATCATCCCAGGCGACAAAGATGCTGTTCCTTC18000.11345822562680943No Hit
CATCAAAAGAGCATTGTCATTAAACACAATGACAAAAGATGCTGAAAGGG17830.11238667571811178No Hit
TCTTTTTAGTTTGCCCCTTTCAGCATCTTTTGTCATTGTGTTTAATGACA17470.1101175112055756No Hit
GTATGGAAGGGATAAATGATTTTTACCGAACATGTAAATTATTGGGAATA17110.1078483466930394No Hit
GTTCATAGCTGATTATAGGTACACATACAAATGCCACAGAGGAGATTCCA17110.1078483466930394No Hit
TAATAATACTGCTGCTCTTTTCAACCACCATGCGTTCTGGTGGTAGGCTG17050.10747015260761669No Hit
CTCCATTTCATGGCTTGGACAATAGGACATTTAAATCAAATAAAAAGAAG16960.10690286147948265No Hit
CACTTACACACACGCGTAACACAGGTTTTAGACGGATACAAGCGCCTTTG16950.1068398291319122No Hit
TGTTTATTCCCAATAATTTACATGTTCGGTAAAAATCATTTATCCCTTCC16780.10576827922321456No Hit
GTATCTAACTTTGCAATGGAAATTCCTTCATTTGGAGTTGCTGGAGTAAA16580.10450763227180557No Hit
GTATTGAAGTACAACCTAATGGACCCTGAATACAAAGGGCGGTTACTTCA16550.10431853522909422No Hit
CTTATGGGATGGACTGCAATCTTCTGATGATTTTGCTTTGTTTGTTAATG16480.10387730879610106No Hit
CTTACACACACGCGTAACACAGGTTTTAGACGGATACAAGCGCCTTTGAA16380.10324698532039657No Hit
GTATTGTTCCGTTCCCGATTAATATTTCTTCATCATTTTTGAATCCTCTT16290.10267969419226254No Hit
ACCAGAGACAGCCCAATTTGGTTCCGGGATTTTTGTAGTATAGCACCTGT16260.10249059714955118No Hit
ACAGAGAGGTATCAATTTTGAGACACAGTTACCAAAAAGAAATTCAGGCT16230.10230150010683983No Hit
GTAAAAATTGAATTTAATGCGATTCTTCTACCTCCAAAACTGTTTCACCC15940.10047356202729678No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTAGGCC100.007091652144.99725145
CGAACCT203.86677E-4108.74795145
GAGCAGA1200.096.7105561
AAACACG7300.096.361135
ACACGAG4300.092.7308047
GAAACAC7700.091.355364
AACACGT3350.090.908136
ACGTGCC1450.089.99839
CGGTGGA259.3767245E-487.025792
ACACGTG3550.085.773027
CACGAGC4650.085.7510768
ACGAGCC2050.084.8764349
ACAACGC8450.080.649368
CTGTAGG554.3823093E-879.126821
GTAGGCA554.3870386E-879.114353
TGTAGGC554.3870386E-879.114352
ACGAGCG1950.078.075459
CAACGCA8900.076.571599
CACGTGC4150.073.37218
AACAACG9600.073.253827
Produced by FastQC (version 0.11.5)