FastQCFastQC Report
Tue 2 Jul 2019
HWC5JBCX2_l01_n01_SM2_9839_P1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWC5JBCX2_l01_n01_SM2_9839_P1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1719019
Sequences flagged as poor quality0
Sequence length151
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATTATTATGTGGTCATTCAATACCTCCATGTTGTCAGAGAGCACTTCCTT91240.530767839098928No Hit
GTATAGTAGCGCTGAGCTTTCATGGCCTTCTGCTATTTCAAATGCTTCAT75560.43955302413760405No Hit
AGCACGCACTTTCTTAAAATGTCACTGTTTGGAGACACAATTGCCTACTT67290.39144418997114055No Hit
TCTATATGCTTTTTAAAACCCAAAGACCAGGAAAGAAAAAGAAGATTCAT50920.2962154577698094No Hit
GTGTGAATGTGATGCTTGTTTCTCGCATAAAGCACAGAGCGTTCCTAGTT46990.27335358131585513No Hit
GCCTTGGAATGGATAAAAAACAAAAGATGCTTAACTGATATACAAAAAGC45980.26747813723990255No Hit
CAATTATTATGTGGTCATTCAATACCTCCATGTTGTCAGAGAGCACTTCC44470.25869405748278523No Hit
TATCTATAATGCTCGAACCATTTCAGATTCTTACAATTTGTTCTTTTATC38110.2216962116183707No Hit
CTACTATACTGTCTCATGGTCATGTACCTGAATCCTGGAAATTATTCGAT35950.20913090547573934No Hit
GATATACAAAAAGCACTAATTGGTGCCTCTATATGCTTTTTAAAACCCAA34570.2011030709957249No Hit
CTGTAGAACATGCTTGTAAATTCAAACATTCCAGTCTCATTACAGTAACT33460.19464589978353933No Hit
CCATTAGAAAGATATAATGAAGAAACAAGGGCAAAATTGAAGAAGCTAAA32120.18685075615801802No Hit
CTCCAAAACTGTTTCACCCATTTTTATTATCTCTTCGGCAGAAAGCCCCT30450.17713591298292805No Hit
TTCAATACCTCCATGTTGTCAGAGAGCACTTCCTTCATTGTTTCTTTGGC29570.17201671418407824No Hit
GTAGTAACAAGAGCATTTTTCAATAACGTTTCTTTGTAATGACAACAAGC29080.16916625121653686No Hit
GTTCTACAGAGATGGATTTGTATCTAATTTTGCAATGGAACTCCCTTCGT28380.16509416126290635No Hit
GTTTATTGTCTCTTTGTTTGGACCTTTTATCCGTATTTTCATGTTTATTC27340.15904419904608386No Hit
CTATAATGCTCGAACCATTTCAGATTCTTACAATTTGTTCTTTTATCTTA27050.15735719035100834No Hit
GCTATGAGCCCTGTGTGAATGTGATGCTTGTTTCTCGCATAAAGCACAGA26850.15619373607854248No Hit
GAATATTACTGCTGCATCTTTAAATGACGATGGATTGGATAATCATACTA26010.15130722813418582No Hit
GTATTTACACAGTCTCTCCAAAGCTTCCATAAGAAATTTTCGTTAGCTTT25610.1489803195892541No Hit
GTTTAATATGCTATCTACCGTGTTGGGAGTAGCTGCACTAGGTATCAAGA25420.14787503803041152No Hit
ATCTTATGCATGGTATTTTCTCAAGACACTAGGATGTTCCAAGGGGTGAG25300.147176965466932No Hit
ATTATGTGGTCATTCAATACCTCCATGTTGTCAGAGAGCACTTCCTTCAT24960.14519909320374003No Hit
AAGTTACACTGTTGGTTTGGTGGGAAAGAATTTGACCTAGACTCAGCCTT24770.14409381164489748No Hit
GTAAGAAATACAGTAAAATTAGATTTAATGCAATTCTTCTATCTCCAAAA24750.14397746621765087No Hit
TTCATGGCCTTCTGCTATTTCAAATGCTTCATGAAAGCTAACACATCTTC23790.13839288570981473No Hit
GAATACAACAATAACCTCTTTTAGGTTGAATGATTTAAATGGAGCCGACA23550.13699674058285569No Hit
CTTAAAATGTCACTGTTTGGAGACACAATTGCCTACTTGCTTTCATTGAC23380.1360078044512597No Hit
GGATGTAATGGAAGTGCTAAAGCAGAGCTCTATGGGAAATTCAGCTCTTG22890.13315734148371833No Hit
ATATTACTCTTAGTGCTTTTCCAAAGGATCCTTTTACTAGTTTTCCTTCT22550.13117946922052637No Hit
GTCTCATGGTCATGTACCTGAATCCTGGAAATTATTCGATGCAAGTAAAA22530.13106312379327975No Hit
AGCAGGCACTTTCTTAAAATGTCACTGTTTGGAGACACAATTGCCTACTT22270.12955063323907415No Hit
CTATATGCTTTTTAAAACCCAAAGACCAGGAAAGAAAAAGAAGATTCATC22130.12873621524834805No Hit
GTATTTTCTCAAGACACTAGGATGTTCCAAGGGGTGAGAGGAGAAATAAA21950.12768910640312878No Hit
GCTTTAGCACTTCCATTACATCCTTTGCAATCCCTTCCCCATTCTTTTGA21650.12594392499442997No Hit
GTAGTAACAAGAGGATTTTTCAATAACGTTTCTTTGTAATGACAACAAGC21200.12332615288138177No Hit
CATAAAGCACAGAGCGTTCCTAGTTTTACTTGCATCGAATAATTTCCAGG21140.12297711659964201No Hit
ACTATACTGTCTCATGGTCATGTACCTGAATCCTGGAAATTATTCGATGC21110.12280259845877213No Hit
GTTCATAGCTGAGACCATCTGCATTTCTCGTCTCACTCCAGGCACTGAAG20510.11931223564137453No Hit
ATACTAAACAGATCAGGACAAGGTATCTGAGCCTTTAGTCTTTTTGTTTT20300.11809060865528537No Hit
ATGCTTAACTGATATACAAAAAGCACTAATTGGTGCCTCTATATGCTTTT20130.11710167252368937No Hit
GGACAATAGGGCATTTGAATCAAATAAAAAGAGGAATAAACATGAAAATA19360.11262237357469579No Hit
GTTGTTCCCATTCCTGATAAGGGCTCTGTGATGAATCTTCTTTTTCTTTC19040.1107608467387504No Hit
GTACTGACCAGCCATTTTCTGAGGAATTATGCTCTCAAATGCTTCAAATG18830.10953921975266126No Hit
TTATAGATATTTCTTCACAAGAGCTGAATTTCCCATAGAGCTCTGCTTTA18680.10866662904831184No Hit
CCATGAGACAGTATAGTAGCGCTGAGCTTTCATGGCCTTCTGCTATTTCA18590.1081430746257022No Hit
CTTCCTTCATTGTTTCTTTGGCCTGGATTTCTTTTTGGTAACTCTGTCTC18560.10796855648483235No Hit
TCTTCATCCTTTGCATTAACAAATAGAGCAAAATCATCAGAAGATTGCAG18320.10657241135787329No Hit
GATTCATTTATCCCATGTAGTTCACTTGCTTTGGGTGATTCCTCATACCC17740.10319839396772229No Hit
CATTCACACAGGGCTCATAGCAGAGCAGCGAGATCTTCAGTGCCTGGAGT17540.10203493969525641No Hit
GTTTAGTATACCATTAGAAAGATATAATGAAGAAACAAGGGCAAAATTGA17470.10162773069989338No Hit
TCATAGATGTGCCCGTACAGGCAGCAATTTCAACAACATTCCCATACACT17410.1012786944181536No Hit
GCACTATACCTTTTCCTTTTCACTACTTTAACTGGTTTTCCTTGATAAAG17320.10075513999554396No Hit
ATGCTACACTGCTTACAAGGTGAAAATTGGAGACAAATATATCACCCAGG17310.10069696728192068No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGCTAC100.007087272145.027571
ACTCATT100.007090355145.00648145
AGCACGC9300.0124.7549061
GCACGCA9300.0124.747652
CACGCAC9400.0123.4205553
ACGCACT10100.0115.5845644
CGCACTT10100.0115.5845645
GTAACAA10250.0109.648614
GAAACAC3250.095.9357454
AACACGT1150.094.574956
GAGCAGA950.091.596371
AAACACG3750.083.144315
TAGAAAC4100.081.3521962
CGTGCTC451.2329365E-680.55916145
ACACGTG1400.077.677537
GCACTTT18500.075.250996
GTTATAT300.001934966572.509573
ACGTGCA1000.072.499039
TAGTAAC15950.070.918452
GAAGCGG1200.066.470971
Produced by FastQC (version 0.11.5)