FastQCFastQC Report
Thu 11 Jul 2019
HWC5JBCX2_l01_n01_SM2_9839_P1_DM2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWC5JBCX2_l01_n01_SM2_9839_P1_DM2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1724288
Sequences flagged as poor quality0
Sequence length151
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATTATTATGTGGTCATTCAATACCTCCATGTTGTCAGAGAGCACTTCCTT91470.5304798270358548No Hit
GTATAGTAGCGCTGAGCTTTCATGGCCTTCTGCTATTTCAAATGCTTCAT75810.43965973201692526No Hit
AGCACGCACTTTCTTAAAATGTCACTGTTTGGAGACACAATTGCCTACTT67420.3910019671887759No Hit
TCTATATGCTTTTTAAAACCCAAAGACCAGGAAAGAAAAAGAAGATTCAT51060.2961222255214906No Hit
GTGTGAATGTGATGCTTGTTTCTCGCATAAAGCACAGAGCGTTCCTAGTT47220.27385216390765343No Hit
GCCTTGGAATGGATAAAAAACAAAAGATGCTTAACTGATATACAAAAAGC46140.2675887090787618No Hit
CAATTATTATGTGGTCATTCAATACCTCCATGTTGTCAGAGAGCACTTCC44600.2586574864523792No Hit
TATCTATAATGCTCGAACCATTTCAGATTCTTACAATTTGTTCTTTTATC38220.22165670700022272No Hit
CTACTATACTGTCTCATGGTCATGTACCTGAATCCTGGAAATTATTCGAT36070.2091877922945587No Hit
GATATACAAAAAGCACTAATTGGTGCCTCTATATGCTTTTTAAAACCCAA34740.2014744636626828No Hit
CTGTAGAACATGCTTGTAAATTCAAACATTCCAGTCTCATTACAGTAACT33550.19457306436047808No Hit
CCATTAGAAAGATATAATGAAGAAACAAGGGCAAAATTGAAGAAGCTAAA32220.18685973572860218No Hit
CTCCAAAACTGTTTCACCCATTTTTATTATCTCTTCGGCAGAAAGCCCCT30550.17717457872466782No Hit
TTCAATACCTCCATGTTGTCAGAGAGCACTTCCTTCATTGTTTCTTTGGC29690.1721870128424022No Hit
GTAGTAACAAGAGCATTTTTCAATAACGTTTCTTTGTAATGACAACAAGC29130.16893929552371761No Hit
GTTCTACAGAGATGGATTTGTATCTAATTTTGCAATGGAACTCCCTTCGT28460.16505363373172No Hit
GTTTATTGTCTCTTTGTTTGGACCTTTTATCCGTATTTTCATGTTTATTC27420.1590221587113058No Hit
CTATAATGCTCGAACCATTTCAGATTCTTACAATTTGTTCTTTTATCTTA27120.15728231014772476No Hit
GCTATGAGCCCTGTGTGAATGTGATGCTTGTTTCTCGCATAAAGCACAGA26910.15606441615321803No Hit
GAATATTACTGCTGCATCTTTAAATGACGATGGATTGGATAATCATACTA26100.15136682503154927No Hit
GTATTTACACAGTCTCTCCAAAGCTTCCATAAGAAATTTTCGTTAGCTTT25700.14904702694677455No Hit
GTTTAATATGCTATCTACCGTGTTGGGAGTAGCTGCACTAGGTATCAAGA25490.14782913295226782No Hit
ATCTTATGCATGGTATTTTCTCAAGACACTAGGATGTTCCAAGGGGTGAG25390.14724918343107415No Hit
ATTATGTGGTCATTCAATACCTCCATGTTGTCAGAGAGCACTTCCTTCAT24990.14492938534629946No Hit
GTAAGAAATACAGTAAAATTAGATTTAATGCAATTCTTCTATCTCCAAAA24830.14400146611238956No Hit
AAGTTACACTGTTGGTTTGGTGGGAAAGAATTTGACCTAGACTCAGCCTT24820.14394347116027023No Hit
TTCATGGCCTTCTGCTATTTCAAATGCTTCATGAAAGCTAACACATCTTC23830.13820197090045283No Hit
GAATACAACAATAACCTCTTTTAGGTTGAATGATTTAAATGGAGCCGACA23640.13710006681018483No Hit
CTTAAAATGTCACTGTTTGGAGACACAATTGCCTACTTGCTTTCATTGAC23400.13570818795932002No Hit
GGATGTAATGGAAGTGCTAAAGCAGAGCTCTATGGGAAATTCAGCTCTTG22970.1332144050181872No Hit
ATATTACTCTTAGTGCTTTTCCAAAGGATCCTTTTACTAGTTTTCCTTCT22620.13118458169400934No Hit
GTCTCATGGTCATGTACCTGAATCCTGGAAATTATTCGATGCAAGTAAAA22580.1309526018855319No Hit
AGCAGGCACTTTCTTAAAATGTCACTGTTTGGAGACACAATTGCCTACTT22330.1295027280825477No Hit
CTATATGCTTTTTAAAACCCAAAGACCAGGAAAGAAAAAGAAGATTCATC22180.1286328038007572No Hit
GTATTTTCTCAAGACACTAGGATGTTCCAAGGGGTGAGAGGAGAAATAAA21990.1275308997104892No Hit
GCTTTAGCACTTCCATTACATCCTTTGCAATCCCTTCCCCATTCTTTTGA21710.12590704105114692No Hit
GTAGTAACAAGAGGATTTTTCAATAACGTTTCTTTGTAATGACAACAAGC21250.123239273253656No Hit
CATAAAGCACAGAGCGTTCCTAGTTTTACTTGCATCGAATAATTTCCAGG21200.12294929849305918No Hit
ACTATACTGTCTCATGGTCATGTACCTGAATCCTGGAAATTATTCGATGC21170.12277531363670106No Hit
GTTCATAGCTGAGACCATCTGCATTTCTCGTCTCACTCCAGGCACTGAAG20550.11917962660530027No Hit
ATACTAAACAGATCAGGACAAGGTATCTGAGCCTTTAGTCTTTTTGTTTT20370.11813571746715167No Hit
ATGCTTAACTGATATACAAAAAGCACTAATTGGTGCCTCTATATGCTTTT20210.11720779823324178No Hit
GGACAATAGGGCATTTGAATCAAATAAAAAGAGGAATAAACATGAAAATA19390.11245221215945365No Hit
GTTGTTCCCATTCCTGATAAGGGCTCTGTGATGAATCTTCTTTTTCTTTC19060.11053837873951451No Hit
GTACTGACCAGCCATTTTCTGAGGAATTATGCTCTCAAATGCTTCAAATG18900.10961045950560463No Hit
TTATAGATATTTCTTCACAAGAGCTGAATTTCCCATAGAGCTCTGCTTTA18740.10868254027169474No Hit
CCATGAGACAGTATAGTAGCGCTGAGCTTTCATGGCCTTCTGCTATTTCA18640.10810259075050108No Hit
CTTCCTTCATTGTTTCTTTGGCCTGGATTTCTTTTTGGTAACTCTGTCTC18610.10792860589414296No Hit
TCTTCATCCTTTGCATTAACAAATAGAGCAAAATCATCAGAAGATTGCAG18370.10653672704327814No Hit
GATTCATTTATCCCATGTAGTTCACTTGCTTTGGGTGATTCCTCATACCC17790.10317301982035484No Hit
CATTCACACAGGGCTCATAGCAGAGCAGCGAGATCTTCAGTGCCTGGAGT17590.10201312077796748No Hit
GTTTAGTATACCATTAGAAAGATATAATGAAGAAACAAGGGCAAAATTGA17540.10172314601737063No Hit
TCATAGATGTGCCCGTACAGGCAGCAATTTCAACAACATTCCCATACACT17480.10137517630465444No Hit
GCACTATACCTTTTCCTTTTCACTACTTTAACTGGTTTTCCTTGATAAAG17380.10079522678346077No Hit
ATGCTACACTGCTTACAAGGTGAAAATTGGAGACAAATATATCACCCAGG17320.10044725707074456No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCACTG100.007086726145.031335
CCTAGCA100.0070885695145.01876
CGCACCC151.2300948E-4145.00189145
AGCACGC8150.0114.809361
CACGCAC8450.0110.704393
GCACGCA8450.0110.704392
GAGCAGA1000.0108.8018951
ATCGAAA203.8663368E-4108.75142145
GAAACAC3950.0106.478694
ACGCACT9150.0103.820244
CGCACTT9350.0100.823915
GTAACAA8900.099.4034964
AAACACG4250.098.962555
TAGAAAC4550.098.8125462
AACACGT1150.094.5774156
ACACGTG1200.090.623567
AACACGA3750.083.144066
ACACGAG3950.078.922797
TCTACTA300.001934322372.515675
GCACTTT17800.070.879936
Produced by FastQC (version 0.11.5)