FastQCFastQC Report
Tue 2 Jul 2019
HWC5JBCX2_l01_n01_SM2_9825_P1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWC5JBCX2_l01_n01_SM2_9825_P1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2402714
Sequences flagged as poor quality0
Sequence length151
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTATAGAACATGCTTGTAAATTCAAACATTCCAGTTTCGTTACAGTAACT123290.5131280710063704No Hit
AGCACGCACTTTCTTAAAATGTCGCTGTTTGGAGACACAATTGCCTACCT95740.39846606795482115No Hit
GTAAGAAATACAGTAAAAATTGAATTTAATGCGATTCTTCTACCTCCAAA84430.3513942982810272No Hit
ATCTAGAGGTTTGCTATGTAATAAGTGACATGAATTTTCTTGACGAAGAA84380.35118620027185926No Hit
CAATAATACAAAAGGCCAAAAACACAATGGCAGAATTTAGTGAAGATCCT71160.2961650866478491No Hit
GTTCTATAGAGATGGATTTGTATCTAACTTTGCAATGGAAATTCCTTCAT69290.28838222110496714No Hit
GTCATAATGGATACTTTTATTACAAGAAACTTCCAGACTACAATAATACA69280.28834060150313356No Hit
GATATACAGAAAGCACTAATTGGTGCCTCTATCTGCTTTTTAAAACCCAA65380.2721089567880322No Hit
TTCTAATACTTTACTTCCCTCCTTTTCAAAACCCAACCATTCATTGAACC60630.2523396459170755No Hit
CTTTTATTACAAGAAACTTCCAGACTACAATAATACAAAAGGCCAAAAAC58330.24276713749534903No Hit
GTATTACCCAAGGGTTGTTACTGATACATTCTTCTATTCCAGAATACATT58060.24164340824584196No Hit
GTAATAAGTGACATGAATTTTCTTGACGAAGAAGGAAAAGCATATACAGC57720.24022834178349983No Hit
ATATAGTAGCGCTGAGCTTTCATGGCCTTCTGCTATTTCAAATGCTTCAT55010.22894942968659607No Hit
GTAGTAACAAGAGCATTTTTCAATAACGTTTCTTTGTAATGATGACAAGC54600.22724302601141874No Hit
ATACTATACTGCTCTATTACTCAACTGCTGCTTCTAGTTTGGCTGTAACA54220.2256614811417422No Hit
ATGCTACACTGCTTACAAGGTGAAAATTGGAGGCAAATATATCACCCAGG50380.20967955403764243No Hit
GCCTTGGAATGGATAAAAAACAAAAGATGCTTAACTGATATACAGAAAGC46960.19544565021055357No Hit
AGTATGCACTCTGTATTACCCAAGGGTTGTTACTGATACATTCTTCTATT46010.19149178803636221No Hit
AGTAGAAACAACGCACTTTTTCCAGTTTATTTGCTGACATTGATTACAAT45960.19128369002719425No Hit
GTGTGAATGTGATGCTTGTTTTTCGCACAAAGCACAGAGTGTTCCTAGTT45220.18820383949150835No Hit
CTACTATATTGTCTCATGGTCATGTACCTGAATCCTGGAAATTATTCAAT41040.1708068459250664No Hit
AGCATATACAGCATTAGAAGGACAAGGGAAAGAACAAAATTTGAGACCAC40930.17034903030489687No Hit
ATTCAACATCTGTGTCCATCTAGAGGTTTGCTATGTAATAAGTGACATGA39930.16618707012153758No Hit
ATACATAAGATTGGAATATGGGACGGAGAAGAGGAGTTCCATGTAAGATG39910.16610383091787037No Hit
GTACTAAATTGAGTACTATGTCTTTTATTCATCCATTATTTCATCTACTG38060.15840420457865564No Hit
GAATAAAGGGGCCAAATAAAGAGACAATAAACAGAGAGGTATCAATTTTG37670.15678104010714553No Hit
GTATAGCACCTGTCTTGTTTTCCAACAAAATAGCCAGATTAGGGAAAGGA35590.14812416292575814No Hit
GCTATGAGCTCTGTGTGAATGTGATGCTTGTTTTTCGCACAAAGCACAGA35360.14716691208358548No Hit
ATTGAGTACTATGTCTTTTATTCATCCATTATTTCATCTACTGATTCTAA34870.1451275515937394No Hit
TTATTATTGTCATTCCTATTGCCATATCTGCTGATTCATTTACTCCAGCA34750.1446281163717363No Hit
CCTCTATTCTCTTACAATCCACAAACAGAAGTCCTAACTATATGCGGCAG34670.14429515955706754No Hit
GTTTAATATGCTATCTACCGTGTTGGGAGTAGCAGCACTAGGCATCAAAA34660.14425353995523396No Hit
ATACTAAATGGAATGAATGCTTAAATCCACGAATCTTTTTGGCTATGACT34400.14317143030756055No Hit
TTATTTCATCTACTGATTCTAATACTTTACTTCCCTCCTTTTCAAAACCC33630.13996672096637386No Hit
CTGTATTACCCAAGGGTTGTTACTGATACATTCTTCTATTCCAGAATACA33530.13955052494803794No Hit
GTATAGTATGGTTATCCAATCCATCATCATTTAAAGATGCAGCAGTAATG32950.13713658804168952No Hit
CTTATGTATTGTTCCGTTCCCAATTAATATTTCTTCATCATTTTTGAATC32810.1365539136160192No Hit
ATTACTATGTGGTCACTCAATACTTCCATGTTGTTAGAGAGTACTTCCTT32350.13463941193167392No Hit
GTATGCACTCTGTATTACCCAAGGGTTGTTACTGATACATTCTTCTATTC32180.13393187870050285No Hit
GTCTTTTATTCATCCATTATTTCATCTACTGATTCTAATACTTTACTTCC31500.1311017457758185No Hit
CCATTAGAAAGATATAATGAAGAAACAAGGGCGAAATTAAAAAGGCTGAA30620.12743922081446232No Hit
ATGTCTTTTATTCATCCATTATTTCATCTACTGATTCTAATACTTTACTT29960.12469232709344516No Hit
GACCTAGACTCTGCCTTGGAATGGATAAAAAACAAAAGATGCTTAACTGA29850.12423451147327565No Hit
CTGTTAAAACAGGCCTCAAAAAGATTGCAACATTTAGCGTAGCATTGTTC29470.1226529666035991No Hit
GCATTAGAAGGACAAGGGAAAGAACAAAATTTGAGACCACAATATGAAGT29220.12161247655775927No Hit
TTGTTAGAGAGTACTTCCTTCATTGCTTCCTTAGCCTGAATTTCTTTTTG29170.1214043785485913No Hit
CCTATAATGTTTGAACCATTCCAGATTCTTTCAATTTGTTCTTTTATTTT29110.12115466093758974No Hit
GCACAGAGTGTTCCTAGTTTTACTTGCATTGAATAATTTCCAGGATTCAG28980.12061360611375303No Hit
CTACAATAATACAAAAGGCCAAAAACACAATGGCAGAATTTAGTGAAGAT28890.1202390296972507No Hit
GCTTTAGCACTTCCATTACATCCTTTGCAATTCCTTCCCCATTTTTCTGA28710.11948987686424603No Hit
GTCTAGGGGTGTCCAAATAAGTCTTTGTGCACATCAATCTTATTTCCGCT28390.11815804960557104No Hit
GACTACAATAATACAAAAGGCCAAAAACACAATGGCAGAATTTAGTGAAG28140.11711755955973122No Hit
GTACTGAAGGGAGGAGGAGAAAATTTCATCGAAGTAAGGAAAGGGTCTCC28130.11707593995789761No Hit
ATGTAATAAGTGACATGAATTTTCTTGACGAAGAAGGAAAAGCATATACA28060.11678460274506246No Hit
ATCCAATGATGACCAATAACCCCATGGACATCTTCGGAGCTTATGTGTAC27460.11428742663504687No Hit
GATTATAGGTACACATACAAATGCCACAGAGGAGATTCCAAAGTGGAAGG27410.1140793286258789No Hit
TCCTAGTGTCTTGGGAAAATACCATGCACAAAATTATTAAATCTTTCATG27380.11395446982037812No Hit
GAACAATACATAAGATTGGAATATGGGACGGAGAAGAGGAGTTCCATGTA27190.11316369738553986No Hit
GTTAGATACAAATCCATCTCTATAGAACATGCTTGTAAATTCAAACATTC26860.11179025052503128No Hit
GTTAAAACAGGCCTCAAAAAGATTGCAACATTTAGCGTAGCATTGTTCTT26780.11145729371036253No Hit
GCACTATATCTTTTCCTTTTAACTACTTTAACGGGTTTTCCTTGATAAAG26610.11074976047919145No Hit
AAATTACACTGTTGGTTCGGTGGGAAAGAATTTGACCTAGACTCTGCCTT26390.10983412923885241No Hit
GTATAGATCTGTTCCTTTTGGTTCTCCAGCTATGAGTTCCAGACACTGCA26260.10929307441501568No Hit
CATTATAGGTATTTCTTCACAAGAGCTGAATTTCCCATAGAGCTCTGCTT26240.1092098352113485No Hit
GTATTTAGTATAGATCTGTTCCTTTTGGTTCTCCAGCTATGAGTTCCAGA26140.10879363919301256No Hit
ATATTACTCTTAGTGCTTTTCCAAAGGATCCTTTTACTAGTTTCCCTTCT26080.10854392158201101No Hit
GTCCTGATCTGTTCAGCATACCATTAGAAAGATATAATGAAGAAACAAGG26060.10846068237834383No Hit
GTTCAGCATACCATTAGAAAGATATAATGAAGAAACAAGGGCGAAATTAA26000.10821096476734227No Hit
CATTCACACAGAGCTCATAGCAGAGCAGCGAGATCCTCAGTGCCAGGAGT25930.10791962755450711No Hit
GTTCATAGCTGAGACCATCTGCATTTCCCGTCTCACTCCTGGCACTGAGG25740.10712885511966884No Hit
ACTATATTGTCTCATGGTCATGTACCTGAATCCTGGAAATTATTCAATGC25180.10479815741698763No Hit
TTCTAATGGTATGCTGAACAGATCAGGACAAGGTATTTGAGCCTTTAGTC25080.10438196139865169No Hit
ATGCTATTCAACATCTGTGTCCATCTAGAGGTTTGCTATGTAATAAGTGA24950.10384090657481498No Hit
GTATTGTTCCGTTCCCAATTAATATTTCTTCATCATTTTTGAATCCTCTT24740.10296689493630952No Hit
CCATTAGGTTGTACTTCAATACTATTTCTGGGATATGTAAGTTTCTCAAA24700.10280041652897515No Hit
GGATAAAGCACTATATCTTTTCCTTTTAACTACTTTAACGGGTTTTCCTT24580.10230098130697203No Hit
GCTATGAACACAGCAAAAACAATGAATGGAATGGGGAAAGGAGAAGACGT24470.1018431656868025No Hit
CTGATATACAGAAAGCACTAATTGGTGCCTCTATCTGCTTTTTAAAACCC24460.10180154608496891No Hit
ATATTGTCTCATGGTCATGTACCTGAATCCTGGAAATTATTCAATGCAAG24130.10042809922446033No Hit
GTTCATAGAAATGCTACACTGCTTACAAGGTGAAAATTGGAGGCAAATAT24080.10022000121529238No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGGACG100.00708249145.061521
TATATAC100.007087781145.025283
CACAGCG100.007087781145.025283
ACGGTTT100.007087781145.025285
CGGTTTA100.0070895455145.013216
AGCACGC10600.0128.639471
GCACGCA10600.0128.607332
CACGCAC10650.0128.003543
GCACAGG256.4611304E-6116.020232
GCACAGC501.8189894E-12116.020232
GAAACAC6500.0110.442344
CGCACTG203.864043E-4108.768975
GAGCAGA1950.0107.866261
AAACACG6850.0104.79935
AACACGT1950.096.675486
ACACGTG2050.091.950037
CAACGCA7400.084.255679
ACAACGC7500.083.132268
AACACGA6450.082.061746
AACAACG7600.081.084487
Produced by FastQC (version 0.11.5)