FastQCFastQC Report
Thu 11 Jul 2019
HWC5JBCX2_l01_n01_SM2_9825_P1_DM2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWC5JBCX2_l01_n01_SM2_9825_P1_DM2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2410537
Sequences flagged as poor quality0
Sequence length151
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTATAGAACATGCTTGTAAATTCAAACATTCCAGTTTCGTTACAGTAACT123740.5133296024910632No Hit
AGCACGCACTTTCTTAAAATGTCGCTGTTTGGAGACACAATTGCCTACCT96020.39833447899783325No Hit
GTAAGAAATACAGTAAAAATTGAATTTAATGCGATTCTTCTACCTCCAAA84660.35120805032239705No Hit
ATCTAGAGGTTTGCTATGTAATAAGTGACATGAATTTTCTTGACGAAGAA84530.3506687514026957No Hit
CAATAATACAAAAGGCCAAAAACACAATGGCAGAATTTAGTGAAGATCCT71380.29611659144829555No Hit
GTTCTATAGAGATGGATTTGTATCTAACTTTGCAATGGAAATTCCTTCAT69600.28873234470161624No Hit
GTCATAATGGATACTTTTATTACAAGAAACTTCCAGACTACAATAATACA69460.28815156124963026No Hit
GATATACAGAAAGCACTAATTGGTGCCTCTATCTGCTTTTTAAAACCCAA65680.27247040804600803No Hit
TTCTAATACTTTACTTCCCTCCTTTTCAAAACCCAACCATTCATTGAACC60820.25230892535563654No Hit
CTTTTATTACAAGAAACTTCCAGACTACAATAATACAAAAGGCCAAAAAC58550.24289193652700625No Hit
GTATTACCCAAGGGTTGTTACTGATACATTCTTCTATTCCAGAATACATT58200.2414399778970412No Hit
GTAATAAGTGACATGAATTTTCTTGACGAAGAAGGAAAAGCATATACAGC57930.2403198955253539No Hit
ATATAGTAGCGCTGAGCTTTCATGGCCTTCTGCTATTTCAAATGCTTCAT55150.2287871955502031No Hit
GTAGTAACAAGAGCATTTTTCAATAACGTTTCTTTGTAATGATGACAAGC54780.22725226785566868No Hit
ATACTATACTGCTCTATTACTCAACTGCTGCTTCTAGTTTGGCTGTAACA54350.2254684329674259No Hit
ATGCTACACTGCTTACAAGGTGAAAATTGGAGGCAAATATATCACCCAGG50550.20970431069923423No Hit
GCCTTGGAATGGATAAAAAACAAAAGATGCTTAACTGATATACAGAAAGC47100.19539214706100755No Hit
AGTAGAAACAACGCACTTTTTCCAGTTTATTTGCTGACATTGATTACAAT46160.19149260102624435No Hit
AGTATGCACTCTGTATTACCCAAGGGTTGTTACTGATACATTCTTCTATT46120.1913266628971055No Hit
GTGTGAATGTGATGCTTGTTTTTCGCACAAAGCACAGAGTGTTCCTAGTT45360.18817383844346716No Hit
CTACTATATTGTCTCATGGTCATGTACCTGAATCCTGGAAATTATTCAAT41170.17079181941617158No Hit
AGCATATACAGCATTAGAAGGACAAGGGAAAGAACAAAATTTGAGACCAC41060.17033548956103972No Hit
ATTCAACATCTGTGTCCATCTAGAGGTTTGCTATGTAATAAGTGACATGA40040.16610406726799878No Hit
ATACATAAGATTGGAATATGGGACGGAGAAGAGGAGTTCCATGTAAGATG40020.16602109820342936No Hit
GTACTAAATTGAGTACTATGTCTTTTATTCATCCATTATTTCATCTACTG38210.15851239785989596No Hit
GAATAAAGGGGCCAAATAAAGAGACAATAAACAGAGAGGTATCAATTTTG37760.15664559390708377No Hit
GTATAGCACCTGTCTTGTTTTCCAACAAAATAGCCAGATTAGGGAAAGGA35680.14801681119186305No Hit
GCTATGAGCTCTGTGTGAATGTGATGCTTGTTTTTCGCACAAAGCACAGA35480.14718712054616875No Hit
ATTGAGTACTATGTCTTTTATTCATCCATTATTTCATCTACTGATTCTAA34930.14490547127050943No Hit
TTATTATTGTCATTCCTATTGCCATATCTGCTGATTCATTTACTCCAGCA34860.14461507954451644No Hit
CCTCTATTCTCTTACAATCCACAAACAGAAGTCCTAACTATATGCGGCAG34780.1442832032862387No Hit
GTTTAATATGCTATCTACCGTGTTGGGAGTAGCAGCACTAGGCATCAAAA34750.14415874968938455No Hit
ATACTAAATGGAATGAATGCTTAAATCCACGAATCTTTTTGGCTATGACT34510.1431631209145514No Hit
TTATTTCATCTACTGATTCTAATACTTTACTTCCCTCCTTTTCAAAACCC33770.14009326552548249No Hit
CTGTATTACCCAAGGGTTGTTACTGATACATTCTTCTATTCCAGAATACA33660.13963693567035063No Hit
GTATAGTATGGTTATCCAATCCATCATCATTTAAAGATGCAGCAGTAATG33090.13727231733012188No Hit
CTTATGTATTGTTCCGTTCCCAATTAATATTTCTTCATCATTTTTGAATC32910.136525595748997No Hit
ATTACTATGTGGTCACTCAATACTTCCATGTTGTTAGAGAGTACTTCCTT32490.134783245393039No Hit
GTATGCACTCTGTATTACCCAAGGGTTGTTACTGATACATTCTTCTATTC32340.13416097740876826No Hit
GTCTTTTATTCATCCATTATTTCATCTACTGATTCTAATACTTTACTTCC31550.1308836993582758No Hit
CCATTAGAAAGATATAATGAAGAAACAAGGGCGAAATTAAAAAGGCTGAA30790.12773087490463744No Hit
ATGTCTTTTATTCATCCATTATTTCATCTACTGATTCTAATACTTTACTT30050.12466101951556852No Hit
GACCTAGACTCTGCCTTGGAATGGATAAAAAACAAAAGATGCTTAACTGA29910.12408023606358251No Hit
CTGTTAAAACAGGCCTCAAAAAGATTGCAACATTTAGCGTAGCATTGTTC29650.12300163822417992No Hit
GCATTAGAAGGACAAGGGAAAGAACAAAATTTGAGACCACAATATGAAGT29330.12167413319106905No Hit
CCTATAATGTTTGAACCATTCCAGATTCTTTCAATTTGTTCTTTTATTTT29260.12138374146507602No Hit
TTGTTAGAGAGTACTTCCTTCATTGCTTCCTTAGCCTGAATTTCTTTTTG29250.12134225693279131No Hit
GCACAGAGTGTTCCTAGTTTTACTTGCATTGAATAATTTCCAGGATTCAG29030.12042959722252759No Hit
CTACAATAATACAAAAGGCCAAAAACACAATGGCAGAATTTAGTGAAGAT29020.12038811269024288No Hit
GCTTTAGCACTTCCATTACATCCTTTGCAATTCCTTCCCCATTTTTCTGA28790.11943396844769442No Hit
GTCTAGGGGTGTCCAAATAAGTCTTTGTGCACATCAATCTTATTTCCGCT28480.11814794794686827No Hit
GACTACAATAATACAAAAGGCCAAAAACACAATGGCAGAATTTAGTGAAG28230.11711083463975039No Hit
GTACTGAAGGGAGGAGGAGAAAATTTCATCGAAGTAAGGAAAGGGTCTCC28210.11702786557518097No Hit
ATGTAATAAGTGACATGAATTTTCTTGACGAAGAAGGAAAAGCATATACA28130.11669598931690324No Hit
ATCCAATGATGACCAATAACCCCATGGACATCTTCGGAGCTTATGTGTAC27550.11428988644438978No Hit
GATTATAGGTACACATACAAATGCCACAGAGGAGATTCCAAAGTGGAAGG27490.11404097925068149No Hit
TCCTAGTGTCTTGGGAAAATACCATGCACAAAATTATTAAATCTTTCATG27470.11395801018611205No Hit
GAACAATACATAAGATTGGAATATGGGACGGAGAAGAGGAGTTCCATGTA27270.11312831954041774No Hit
GTTAGATACAAATCCATCTCTATAGAACATGCTTGTAAATTCAAACATTC26960.11184229903959159No Hit
GTTAAAACAGGCCTCAAAAAGATTGCAACATTTAGCGTAGCATTGTTCTT26830.1113030001198903No Hit
GCACTATATCTTTTCCTTTTAACTACTTTAACGGGTTTTCCTTGATAAAG26690.11072221666790429No Hit
AAATTACACTGTTGGTTCGGTGGGAAAGAATTTGACCTAGACTCTGCCTT26460.10976807242535583No Hit
GTATAGATCTGTTCCTTTTGGTTCTCCAGCTATGAGTTCCAGACACTGCA26390.10947768069936284No Hit
CATTATAGGTATTTCTTCACAAGAGCTGAATTTCCCATAGAGCTCTGCTT26290.10906283537651569No Hit
GTATTTAGTATAGATCTGTTCCTTTTGGTTCTCCAGCTATGAGTTCCAGA26220.10877244365052269No Hit
ATATTACTCTTAGTGCTTTTCCAAAGGATCCTTTTACTAGTTTCCCTTCT26180.10860650552138383No Hit
GTCCTGATCTGTTCAGCATACCATTAGAAAGATATAATGAAGAAACAAGG26170.10856502098909912No Hit
GTTCAGCATACCATTAGAAAGATATAATGAAGAAACAAGGGCGAAATTAA26060.10810869113396726No Hit
CATTCACACAGAGCTCATAGCAGAGCAGCGAGATCCTCAGTGCCAGGAGT26010.10790126847254368No Hit
GTTCATAGCTGAGACCATCTGCATTTCCCGTCTCACTCCTGGCACTGAGG25790.10698860876227993No Hit
ACTATATTGTCTCATGGTCATGTACCTGAATCCTGGAAATTATTCAATGC25250.10474844401890535No Hit
TTCTAATGGTATGCTGAACAGATCAGGACAAGGTATTTGAGCCTTTAGTC25160.1043750832283429No Hit
ATGCTATTCAACATCTGTGTCCATCTAGAGGTTTGCTATGTAATAAGTGA25040.10387726884092631No Hit
CCATTAGGTTGTACTTCAATACTATTTCTGGGATATGTAAGTTTCTCAAA24760.1027157019369543No Hit
GTATTGTTCCGTTCCCAATTAATATTTCTTCATCATTTTTGAATCCTCTT24760.1027157019369543No Hit
GGATAAAGCACTATATCTTTTCCTTTTAACTACTTTAACGGGTTTTCCTT24670.10234234114639186No Hit
GCTATGAACACAGCAAAAACAATGAATGGAATGGGGAAAGGAGAAGACGT24540.10180304222669058No Hit
CTGATATACAGAAAGCACTAATTGGTGCCTCTATCTGCTTTTTAAAACCC24530.10176155769440584No Hit
GTTCATAGAAATGCTACACTGCTTACAAGGTGAAAATTGGAGGCAAATAT24220.10047553719357968No Hit
ATATTGTCTCATGGTCATGTACCTGAATCCTGGAAATTATTCAATGCAAG24210.10043405266129497No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTAACGG100.0070851506145.04331
CGGAAGT100.0070886672145.019235
CGAAGCG100.0070886672145.019234
AGCCACC100.0070913057145.00119145
CGCTGCC151.230179E-4145.00117145
AGCACGC11450.0126.0421
GCACGCA11850.0121.1553042
CACGCAC11850.0120.543413
CGGTGCC203.8646866E-4108.764432
GAAACAC7950.0103.976054
AAACACG8300.0100.465135
GAGCAGA1800.096.695541
AACACGT2550.090.986796
AAGCGGT1200.090.637022
AACACGA7150.084.166766
GAAGCGG3550.081.714531
ACACGTG2850.081.402497
ACAACGC8650.080.461428
CAACGCA8800.079.089919
ACACGAG7650.078.659147
Produced by FastQC (version 0.11.5)