FastQCFastQC Report
Tue 2 Jul 2019
HWC5JBCX2_l01_n01_SM2_9823_P1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWC5JBCX2_l01_n01_SM2_9823_P1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1567594
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTACTATACTGTCTCATGGTCATGTACCTGAATCCTGGAAATTATTCAAT82530.5264756052906556No Hit
GTATAGTAGCGCTGAGCTTTCATGGCCTTCTGCTATTTCAAATGCTTCAT65910.4204532551158016No Hit
ATTATTATGTGGTCATTCAATACCTCCATGTTGTCAGAGAGTACTTCCTT59360.37866947691813063No Hit
TCTATATGCTTTTTAAAACCCAAAGACCAGGAAAGAAAAAGAAGATTCAT47010.29988632260649123No Hit
GTTCATAGCTGAGACCATTTGCATTTCTCGTCTCACTCCAGGCACTGAAG44850.2861072446054272No Hit
CATTCACACAGGGCTCATAGCAGAGCAGCGAGATCTTCAGTGCCTGGAGT44090.2812590504939417No Hit
GTAAGAAATACAGTAAAATTGAATTTAATGCAATTCTTCTATCTCCAAAA40200.25644395168646983No Hit
GTCTCATGGTCATGTACCTGAATCCTGGAAATTATTCAATGCAAGTAAAA40190.2563801596586871No Hit
GCCTTGGAATGGATAAAAAACAAAAGATGCTTAACTGATATACAAAAGGC39680.2531267662417693No Hit
GCTATGAACACAGCAAAAACAATGAATGGAATGGGAAAAGGAGAAGACGT39370.2511492133805054No Hit
GTGTGAATGTGATGCTTGTTTCTCGCATAAAGCACAGAGCGTTCCTAGTT39030.24898028443589348No Hit
TTATAGATATTTCTTCACAAGAGCTGAATTTCCCATGGAGCTCTGCTTTA37120.23679600712939705No Hit
GCTTTAGCACTTCCATTACATCCTTTGCAATCCCTTCCCCATTCTTTTGA35970.22945992393438608No Hit
ACTATACTGTCTCATGGTCATGTACCTGAATCCTGGAAATTATTCAATGC34990.2232083052116811No Hit
GCTATGAGCCCTGTGTGAATGTGATGCTTGTTTCTCGCATAAAGCACAGA34800.2219962566838097No Hit
CTCCAATGTTGCTTTGTAGCTCTTCTGCCAGCTTTTGGACGTCTTCTCCT30850.19679840570964163No Hit
GTCATGTACCTGAATCCTGGAAATTATTCAATGCAAGTAAAACTAGGAAC30350.19360880432050645No Hit
CAATTATTATGTGGTCATTCAATACCTCCATGTTGTCAGAGAGTACTTCC29360.18729339357001878No Hit
ATACTGTCTCATGGTCATGTACCTGAATCCTGGAAATTATTCAATGCAAG28840.18397620812531817No Hit
CTATTGTCCAAGCCATGAAATGGAGAGCTGATAAGATAAAAGAACAAATT26140.1667523606239881No Hit
GTTTATTGTCTCTTTGTTTGGACCTTTTATTCGTATTTTCATGTTTATTC25510.16273346287367776No Hit
GCTCATAGCAGAGCAGCGAGATCTTCAGTGCCTGGAGTGAGACGAGAAAT25430.1622231266514161No Hit
CCATTACATCCTTTGCAATCCCTTCCCCATTCTTTTGACTTGCCCCAAGA24910.15890594120671553No Hit
ATTACATCCTTTGCAATCCCTTCCCCATTCTTTTGACTTGCCCCAAGAGA24780.15807664484554035No Hit
TTGCAATCCCTTCCCCATTCTTTTGACTTGCCCCAAGAGATCTCAGCACT24060.15348361884518566No Hit
GATATACAAAAGGCACTAATTGGTGCCTCTATATGCTTTTTAAAACCCAA24020.15322845073405486No Hit
GACCTAGACTCTGCCTTGGAATGGATAAAAAACAAAAGATGCTTAACTGA23770.15163365003948728No Hit
TATCTATAATGCTCGAACCATTTCAGATTCTTTCAATTTGTTCTTTTATC23630.1507405616505294No Hit
CTTTTGACTTGCCCCAAGAGATCTCAGCACTCCAATGTTGCTTTGTAGCT23020.14684924795578447No Hit
AAATTACACTGTTGGTTTGGTGGGAAAGAATTTGACCTAGACTCTGCCTT22880.14595615956682662No Hit
CTCCAAAACTGTTTCACCCATTTTTATTATCTCTTCGGCAGAAAGCCCCT22530.14372343859443198No Hit
GTGCTTTATGCGAGAAACAAGCATCACATTCACACAGGGCTCATAGCAGA21660.13817353217733674No Hit
TTCAATACCTCCATGTTGTCAGAGAGTACTTCCTTCATTGTTTCTTTGGC21610.13785457203842322No Hit
ATTCATCACAGAGCCCTTATCAGGAATGGGAACAACAGCAACAAAAAAGA20870.13313396198250313No Hit
CTGTAGAACATGCTTGTAAATTCAAACATTCCAGTCTCATTACAGTAACT20470.13058228087119497No Hit
GTTGGTTTGGTGGGAAAGAATTTGACCTAGACTCTGCCTTGGAATGGATA20390.13007194464893332No Hit
CTATATGCTTTTTAAAACCCAAAGACCAGGAAAGAAAAAGAAGATTCATC19530.12458583025962079No Hit
CCATGAGACAGTATAGTAGCGCTGAGCTTTCATGGCCTTCTGCTATTTCA19490.12433066214848998No Hit
GCCCTTATCAGGAATGGGAACAACAGCAACAAAAAAGAAAGGCCTGATTC19240.12273586145392237No Hit
TCATAGATGTGCCCGTACAGGCAGCAATTTCAACAACATTCCCATACACT19220.12260827739835697No Hit
ACATTGGAGTGCTGAGATCTCTTGGGGCAAGTCAAAAGAATGGGGAAGGG19060.12158760495383371No Hit
GTCCAAGCCATGAAATGGAGAGCTGATAAGATAAAAGAACAAATTGAAAG18740.11954626006478719No Hit
CTGATATACAAAAGGCACTAATTGGTGCCTCTATATGCTTTTTAAAACCC18520.11814283545356771No Hit
TAGCAGAGCAGCGAGATCTTCAGTGCCTGGAGTGAGACGAGAAATGCAAA18440.11763249923130606No Hit
AGCAGGCACTTTCTTAAAATGTCGCTGTTTGGAGACACAATTGCCTACCT18350.11705837098126173No Hit
GTTTAGTATACCGTTAGAAAGATATAATGAAGAAACAAGGGCAAAATTGA17980.11469806595330169No Hit
GCCCTATTGTCCAAGCCATGAAATGGAGAGCTGATAAGATAAAAGAACAA17910.11425152175882276No Hit
GTATAGCACCGGTCCTGTTCTCCAATAAGATAGCAAGATTGGGGAAAGGG17820.11367739350877841No Hit
CTTTATTTGATTCAAATGCCCTATTGTCCAAGCCATGAAATGGAGAGCTG17610.11233776092534165No Hit
TTCTTAAAATGTCGCTGTTTGGAGACACAATTGCCTACCTGCTTTCATTG17070.10889299142507562No Hit
GCACTATACCTTTTCCTTTTAACTACTTTAACTGGTTTTCCTTGATAAAG16840.10742577478607344No Hit
GCACAGAGCGTTCCTAGTTTTACTTGCATTGAATAATTTCCAGGATTCAG16730.10672406248046369No Hit
AAATTATTCAATGCAAGTAAAACTAGGAACGCTCTGTGCTTTATGCGAGA16730.10672406248046369No Hit
CTATAATGCTCGAACCATTTCAGATTCTTTCAATTTGTTCTTTTATCTTA16660.10627751828598477No Hit
ATACTAAACAGATCAGGACAAGGTATTTGAGCCTTCAGTCTTTTTGTTTT16490.10519305381367879No Hit
ATTATGTGGTCATTCAATACCTCCATGTTGTCAGAGAGTACTTCCTTCAT16340.10423617339693823No Hit
CCCCAAGAGATCTCAGCACTCCAATGTTGCTTTGTAGCTCTTCTGCCAGC16300.10398100528580742No Hit
GTGCTATACTACAAAAATCCCTGAACCAAATTGGGCTGTCTCTGGTTATT16060.10244999661902254No Hit
CCCATACACTGGTGTTCCCCCTTATTCTCATGGAACAGGAACAGGCTACA15970.10187586836897819No Hit
ATGCTTAACTGATATACAAAAGGCACTAATTGGTGCCTCTATATGCTTTT15900.10142932417449926No Hit
CTCTTGGGGCAAGTCAAAAGAATGGGGAAGGGATTGCAAAGGATGTAATG15900.10142932417449926No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACACGT1600.0117.819186
ACACGTG1600.0117.811677
TAACACG256.4642045E-6116.006585
ACGTGCC750.0115.999189
GAAACAC3650.0111.239194
GAGCAGA1200.0108.7631151
AAACACG3750.0108.272815
CACGTGC1800.0104.721488
ACGAGCC1400.0103.57079
AACACGA2650.093.024146
ACGTGCA1100.092.272089
ACACGAG2700.091.2956547
CAACGCA2200.088.976659
AGCACGC1400.082.8671341
GCACGCA1450.080.004542
TAGAAAC5350.078.602592
CACGAGC3150.078.253428
GTAACAA3450.077.758034
CACGCAC1500.077.337723
TAGTAGA702.8121576E-972.504123
Produced by FastQC (version 0.11.5)