FastQCFastQC Report
Thu 11 Jul 2019
HWC5JBCX2_l01_n01_SM2_9823_P1_DM2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWC5JBCX2_l01_n01_SM2_9823_P1_DM2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1575072
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTACTATACTGTCTCATGGTCATGTACCTGAATCCTGGAAATTATTCAAT82920.5264521240933748No Hit
GTATAGTAGCGCTGAGCTTTCATGGCCTTCTGCTATTTCAAATGCTTCAT66170.42010777919993497No Hit
ATTATTATGTGGTCATTCAATACCTCCATGTTGTCAGAGAGTACTTCCTT59540.37801446537047195No Hit
TCTATATGCTTTTTAAAACCCAAAGACCAGGAAAGAAAAAGAAGATTCAT47290.3002402429857175No Hit
GTTCATAGCTGAGACCATTTGCATTTCTCGTCTCACTCCAGGCACTGAAG44990.2856377359257227No Hit
CATTCACACAGGGCTCATAGCAGAGCAGCGAGATCTTCAGTGCCTGGAGT44330.2814474512911156No Hit
GTAAGAAATACAGTAAAATTGAATTTAATGCAATTCTTCTATCTCCAAAA40410.2565597001279942No Hit
GTCTCATGGTCATGTACCTGAATCCTGGAAATTATTCAATGCAAGTAAAA40360.2562422543223421No Hit
GCCTTGGAATGGATAAAAAACAAAAGATGCTTAACTGATATACAAAAGGC39830.2528773287824303No Hit
GCTATGAACACAGCAAAAACAATGAATGGAATGGGAAAAGGAGAAGACGT39530.2509726539485179No Hit
GTGTGAATGTGATGCTTGTTTCTCGCATAAAGCACAGAGCGTTCCTAGTT39220.24900448995347513No Hit
TTATAGATATTTCTTCACAAGAGCTGAATTTCCCATGGAGCTCTGCTTTA37370.23725899514434895No Hit
GCTTTAGCACTTCCATTACATCCTTTGCAATCCCTTCCCCATTCTTTTGA36140.2294498283253083No Hit
ACTATACTGTCTCATGGTCATGTACCTGAATCCTGGAAATTATTCAATGC35180.22335486885678876No Hit
GCTATGAGCCCTGTGTGAATGTGATGCTTGTTTCTCGCATAAAGCACAGA34970.22202159647305014No Hit
CTCCAATGTTGCTTTGTAGCTCTTCTGCCAGCTTTTGGACGTCTTCTCCT30920.19630848621523334No Hit
GTCATGTACCTGAATCCTGGAAATTATTCAATGCAAGTAAAACTAGGAAC30480.1935149631254952No Hit
CAATTATTATGTGGTCATTCAATACCTCCATGTTGTCAGAGAGTACTTCC29510.18735651449584528No Hit
ATACTGTCTCATGGTCATGTACCTGAATCCTGGAAATTATTCAATGCAAG28980.18399158895593345No Hit
CTATTGTCCAAGCCATGAAATGGAGAGCTGATAAGATAAAAGAACAAATT26250.166659047967331No Hit
GTTTATTGTCTCTTTGTTTGGACCTTTTATTCGTATTTTCATGTTTATTC25620.16265923081611508No Hit
GCTCATAGCAGAGCAGCGAGATCTTCAGTGCCTGGAGTGAGACGAGAAAT25500.16189736088255013No Hit
CCATTACATCCTTTGCAATCCCTTCCCCATTCTTTTGACTTGCCCCAAGA24970.1585324353426383No Hit
ATTACATCCTTTGCAATCCCTTCCCCATTCTTTTGACTTGCCCCAAGAGA24880.1579610328924646No Hit
TTGCAATCCCTTCCCCATTCTTTTGACTTGCCCCAAGAGATCTCAGCACT24210.153707259096727No Hit
GATATACAAAAGGCACTAATTGGTGCCTCTATATGCTTTTTAAAACCCAA24130.1531993458076837No Hit
GACCTAGACTCTGCCTTGGAATGGATAAAAAACAAAAGATGCTTAACTGA23890.15167560594055385No Hit
TATCTATAATGCTCGAACCATTTCAGATTCTTTCAATTTGTTCTTTTATC23770.15091373600698887No Hit
CTTTTGACTTGCCCCAAGAGATCTCAGCACTCCAATGTTGCTTTGTAGCT23140.14691391885577296No Hit
AAATTACACTGTTGGTTTGGTGGGAAAGAATTTGACCTAGACTCTGCCTT22930.1455806464720343No Hit
CTCCAAAACTGTTTCACCCATTTTTATTATCTCTTCGGCAGAAAGCCCCT22620.14361248247699154No Hit
GTGCTTTATGCGAGAAACAAGCATCACATTCACACAGGGCTCATAGCAGA21760.13815241461977612No Hit
TTCAATACCTCCATGTTGTCAGAGAGTACTTCCTTCATTGTTTCTTTGGC21720.13789845797525446No Hit
ATTCATCACAGAGCCCTTATCAGGAATGGGAACAACAGCAACAAAAAAGA20960.13307328172934318No Hit
CTGTAGAACATGCTTGTAAATTCAAACATTCCAGTCTCATTACAGTAACT20570.1305972044452571No Hit
GTTGGTTTGGTGGGAAAGAATTTGACCTAGACTCTGCCTTGGAATGGATA20500.13015278031734423No Hit
CTATATGCTTTTTAAAACCCAAAGACCAGGAAAGAAAAAGAAGATTCATC19590.12437526665447673No Hit
CCATGAGACAGTATAGTAGCGCTGAGCTTTCATGGCCTTCTGCTATTTCA19570.12424828833221592No Hit
TCATAGATGTGCCCGTACAGGCAGCAATTTCAACAACATTCCCATACACT19360.12291501594847729No Hit
GCCCTTATCAGGAATGGGAACAACAGCAACAAAAAAGAAAGGCCTGATTC19320.12266105930395563No Hit
ACATTGGAGTGCTGAGATCTCTTGGGGCAAGTCAAAAGAATGGGGAAGGG19140.12151825440360822No Hit
GTCCAAGCCATGAAATGGAGAGCTGATAAGATAAAAGAACAAATTGAAAG18860.11974055789195669No Hit
CTGATATACAAAAGGCACTAATTGGTGCCTCTATATGCTTTTTAAAACCC18670.11853426383047887No Hit
TAGCAGAGCAGCGAGATCTTCAGTGCCTGGAGTGAGACGAGAAATGCAAA18500.11745494809126185No Hit
AGCAGGCACTTTCTTAAAATGTCGCTGTTTGGAGACACAATTGCCTACCT18430.11701052396334898No Hit
GTTTAGTATACCGTTAGAAAGATATAATGAAGAAACAAGGGCAAAATTGA18050.11459793584039334No Hit
GCCCTATTGTCCAAGCCATGAAATGGAGAGCTGATAAGATAAAAGAACAA18020.1144074683570021No Hit
GTATAGCACCGGTCCTGTTCTCCAATAAGATAGCAAGATTGGGGAAAGGG17860.1133916417789155No Hit
CTTTATTTGATTCAAATGCCCTATTGTCCAAGCCATGAAATGGAGAGCTG17710.11243930436195931No Hit
TTCTTAAAATGTCGCTGTTTGGAGACACAATTGCCTACCTGCTTTCATTG17150.10888391133865626No Hit
GCACTATACCTTTTCCTTTTAACTACTTTAACTGGTTTTCCTTGATAAAG16850.1069792365047439No Hit
AAATTATTCAATGCAAGTAAAACTAGGAACGCTCTGTGCTTTATGCGAGA16810.10672527986022226No Hit
GCACAGAGCGTTCCTAGTTTTACTTGCATTGAATAATTTCCAGGATTCAG16790.10659830153796145No Hit
CTATAATGCTCGAACCATTTCAGATTCTTTCAATTTGTTCTTTTATCTTA16710.10609038824891816No Hit
ATACTAAACAGATCAGGACAAGGTATTTGAGCCTTCAGTCTTTTTGTTTT16600.10539200747648361No Hit
CCCCAAGAGATCTCAGCACTCCAATGTTGCTTTGTAGCTCTTCTGCCAGC16430.10431269173726662No Hit
ATTATGTGGTCATTCAATACCTCCATGTTGTCAGAGAGTACTTCCTTCAT16420.10424920257613621No Hit
GTGCTATACTACAAAAATCCCTGAACCAAATTGGGCTGTCTCTGGTTATT16140.10247150606448466No Hit
CCCATACACTGGTGTTCCCCCTTATTCTCATGGAACAGGAACAGGCTACA16020.10170963613091973No Hit
CTCTTGGGGCAAGTCAAAAGAATGGGGAAGGGATTGCAAAGGATGTAATG16000.10158265780865891No Hit
ATGCTTAACTGATATACAAAAGGCACTAATTGGTGCCTCTATATGCTTTT15930.10113823368074604No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCAGA1200.0126.941151
GAAACAC3550.0114.4040154
TAACACG203.8613915E-4108.7859655
AACACGT1550.0107.6059656
ACGTGCC553.6379788E-12105.452589
ACACGTG1600.0104.216817
CACGTGC1600.0104.216818
AAACACG3900.0104.1375
ACGTGCA1050.0103.5694969
GTAACAA3550.098.0605854
ATATGCC301.5998941E-596.66486145
AACACGA2750.094.931446
ACACGAG2750.094.9073267
TCACTGG259.388451E-486.998387
ACCCACA259.388451E-486.998389
AGCACGC1850.086.261161
CACGAGC3150.082.8556068
CAACGCA2400.081.560989
TAGAAAC5450.079.845452
CACGCAC2000.079.778913
Produced by FastQC (version 0.11.5)