FastQCFastQC Report
Thu 11 Jul 2019
HWC5JBCX2_l01_n01_SM2_9815_P1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWC5JBCX2_l01_n01_SM2_9815_P1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences320925
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATACTAGAGTCCCGTTTCCGTTTCATTACCAACACCACGTCTCCTTGC12080.3764119342525512No Hit
CTTTTAAGGCCGTGTTTGAAGAGTCGATAGACGCATTTGAAAAAAAGACG11940.37204954428604814No Hit
GTATTAATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCTGTCAG11480.3577159772532523No Hit
ATTATATTCAGCATGGAAAGAATAAAAGAACTACGGAATCTAATGTCGCA10710.3337228324374854No Hit
GCTATAACTATTTTAATGCATGTGTAAGGAAGGAGTTGAACCAAGACGCA10360.3228168575212277No Hit
ACTCTATGCTGACAAAATGACTGTCGTCAGCATCCACAGCATTCTGCTGT6970.21718470047518892No Hit
GTATAGGAAACTTAAGAGGGAGATAACGTTCCACGGGGCCAAAGAAATAG6870.21406870764197244No Hit
GTACTGGAACTAGGATGAGTCCCAATGGCTCTCATTGCCTGCACCATCTG6840.21313390979200747No Hit
TTTTTGGACAGTACGGATAACAAATAGTAGCACTGCTATAACTATTTTAA6810.21219911194204255No Hit
TTTTTAAACTATTCAGTATTAATTGATGGCCATCCGAATTCTTTTGGTCG6720.2093947183921477No Hit
TTCCAGTACTGGTCTAAGAGATGATCTTCTTGAAAATTTGCAGACCTATC5790.1804159850432344No Hit
TTCCAACACTGTGTCAAGTTTCCAGGTAGATTGCTTTCTTTGGCATATCC5660.17636519436005296No Hit
TTCTTAGACAGAACCCGACTGAAGAACAAGCTGTGGATATATGCAAGGCT5260.16390122302718704No Hit
GTATTATTATCTCCTGAGGAGGTCAGTGAAACACAGGGAACTGAAAGACT5190.16172002804393548No Hit
ATTTTAATGCATGTGTAAGGAAGGAGTTGAACCAAGACGCATTGAGCAAA4950.15424164524421594No Hit
TATCAAAAGAGGGCACGATCGGGTTCGTTGCCTTCTCGTCTGAGAGCTCG4730.14738646101113967No Hit
ATCCCATATTATCCATGTTCTCATTTGAAGCAATTTGTACTCCTCTAGTT4700.14645166316117472No Hit
CTCATAGACTCAGGTACTCCTTCCGTAGAAGGGCCTCTTTTAAGGCCGTG4640.14458206746124483No Hit
CTTCTGCATTGTCTCCGAAGAAATAAGATCCTTCGTTACTCATATCAAAA4540.14146607462802835No Hit
GAATAAAAGAACTACGGAATCTAATGTCGCAGTCTCGCACTCGCGAGATA4390.13679208537820364No Hit
CTCCAACTCTATGCTGACAAAATGACTGTCGTCAGCATCCACAGCATTCT4360.1358572875282387No Hit
ATCTAATGTCGCAGTCTCGCACTCGCGAGATACTGACAAAAACCACAGTG4320.13461089039495208No Hit
ATATGGGATCGAGCACTCTTGAACTGAGAAGCGGGTACTGGGCCATAAGG4320.13461089039495208No Hit
ATATACAGGAGAGTCGATGGAAAATGGATGAGGGAACTCGTCCTTTATGA4300.13398769182830877No Hit
GTTCATTGATGCTTAATGCTGGCCCGTATCTTCTGTCTTCTTTACCTATA4240.1321180961283789No Hit
CCTCAATAGTATGTCCTGGAAAAGAAGGCAATGGTGAGATTTCGCCAACA4230.13180649684505727No Hit
GTATTGGCTTCTAGTGGCCTTGGGGACTAAAGATTGAAATGGTTCAAATT4210.13118329827841396No Hit
GCTTAATGCTGGCCCGTATCTTCTGTCTTCTTTACCTATAATGAGGAACC4190.13056009971177066No Hit
AGCAGGTACTGATTCAAAATGGAAGATTTTGTGCGACAATGCTTCAACCC4180.13024850042844902No Hit
TTCCTATACAGTTTAACTGCTTTGTCCATGTTATTTGGGTCTCCATTCCC4090.12744410687855418No Hit
GTCTAAGAATGTCCACCATCCTTGTTCCGCCAATTTGCGTGCTGTGGCAC3990.1243281140453377No Hit
ATTCAGTATTAATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCT3950.1230817169120511No Hit
CCTATATGAACTACTAGGGAAAAATTTCTCGAACAAGTTGCAGCACTTCT3940.12277011762872946No Hit
ATAATGAGGAACCCTCTCAAGACGGCGGACTCCACTCCGGATGTGCTTTC3920.12214691906208615No Hit
TAATTAAGAAGTACACATCGGGGAGACAGGAAAAGAACCCGTCACTTAGG3860.12027732336215627No Hit
CAATAGGATGGGAGCTGTAACCACTGAAGTGGCATTTGGCCTGGTGTGTG3780.1177845290955831No Hit
CCTATAATGAGGAACCCTCTCAAGACGGCGGACTCCACTCCGGATGTGCT3770.11747292981226143No Hit
CCCTTATACTGGAGATCCTCCATACAGCCATGGAACAGGAACAGGGTACA3750.11684973124561812No Hit
GTACGGATAACAAATAGTAGCACTGCTATAACTATTTTAATGCATGTGTA3660.11404533769572331No Hit
CTATTGAGGATGTCAAAAATGCAATTGGGGTCCTCATCGGAGGACTTGAA3640.11342213912908No Hit
GATATTGAGAGGATCAGTTGCTCACAAATCTTGCCTACCTGCCTGTGCAT3510.10937134844589858No Hit
GTACTGGAGTATTCATCCACACCCATTTTGCTGATTCTTATTCCTCTCAT3500.10905974916257692No Hit
TAATAATACATTCCCACGTTGGTCTCGAACTCTCAAAAACCGATCAATGC3440.10719015346264704No Hit
CTCTTAGACCAGTACTGGAACTAGGATGAGTCCCAATGGCTCTCATTGCC3400.10594375632936043No Hit
TAGTAGCACTGCTATAACTATTTTAATGCATGTGTAAGGAAGGAGTTGAA3340.10407416062943055No Hit
TCCTAGTTCCAGTACTGGTCTAAGAGATGATCTTCTTGAAAATTTGCAGA3270.10189296564617902No Hit
TCTCAAGACGGCGGACTCCACTCCGGATGTGCTTTCATCTGGGTCTTCAA3270.10189296564617902No Hit
ATATACAATAGGATGGGAGCTGTAACCACTGAAGTGGCATTTGGCCTGGT3250.10126976707953571No Hit
GGCCTGGTGTGTGCAACATGTGAGCAGATTGCTGATTCCCAGCACAGGTC3240.10095816779621407No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCATAA100.007088794144.9989145
GCAGGGG100.007088794144.99898
AGCGAAA850.0119.466711
CGAAAGC1150.088.287723
CAGTACG1900.087.762499
TACTGAT703.092282E-1182.8565147
GTACTGA755.638867E-1177.332756
ACAGTAC2300.072.499458
AATTCTA300.001934598272.499455
AAACTAT1750.066.285226
TATATTC2800.064.751843
TTATATT2800.064.751842
ACTATTC1800.064.443968
AGGGTGA350.003564403662.1423876
CTATTCA1900.061.052179
AATTATA1550.060.8059965
CAATTAT1550.060.8059964
ACAAGGT1800.060.4162147
ATATTCA3000.060.4162144
CAGCATG3050.059.425789
Produced by FastQC (version 0.11.5)