FastQCFastQC Report
Thu 11 Jul 2019
HWC5JBCX2_l01_n01_SM2_9815_P1_DM2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWC5JBCX2_l01_n01_SM2_9815_P1_DM2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2055714
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATACTAGAGTCCCGTTTCCGTTTCATTACCAACACCACGTCTCCTTGC80350.3908617638445815No Hit
GTATTAATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCTGTCAG72790.3540862201648673No Hit
CTTTTAAGGCCGTGTTTGAAGAGTCGATAGACGCATTTGAAAAAAAGACG72160.3510215915248911No Hit
ATTATATTCAGCATGGAAAGAATAAAAGAACTACGGAATCTAATGTCGCA69780.3394441055516477No Hit
GCTATAACTATTTTAATGCATGTGTAAGGAAGGAGTTGAACCAAGACGCA64660.31453791723946034No Hit
ACTCTATGCTGACAAAATGACTGTCGTCAGCATCCACAGCATTCTGCTGT46590.22663658466109587No Hit
GTATAGGAAACTTAAGAGGGAGATAACGTTCCACGGGGCCAAAGAAATAG44150.21476722929356906No Hit
TTTTTGGACAGTACGGATAACAAATAGTAGCACTGCTATAACTATTTTAA42730.20785965362886083No Hit
GTACTGGAACTAGGATGAGTCCCAATGGCTCTCATTGCCTGCACCATCTG42640.20742184953743567No Hit
TTTTTAAACTATTCAGTATTAATTGATGGCCATCCGAATTCTTTTGGTCG41640.20255735963271154No Hit
TTCCAGTACTGGTCTAAGAGATGATCTTCTTGAAAATTTGCAGACCTATC38510.18733150623092512No Hit
TTCCAACACTGTGTCAAGTTTCCAGGTAGATTGCTTTCTTTGGCATATCC36080.17551079576244555No Hit
GTATTATTATCTCCTGAGGAGGTCAGTGAAACACAGGGAACTGAAAGACT32830.15970120357209222No Hit
TTCTTAGACAGAACCCGACTGAAGAACAAGCTGTGGATATATGCAAGGCT32790.15950662397590326No Hit
TATCAAAAGAGGGCACGATCGGGTTCGTTGCCTTCTCGTCTGAGAGCTCG31330.15240446871500607No Hit
ATCCCATATTATCCATGTTCTCATTTGAAGCAATTTGTACTCCTCTAGTT31310.1523071789169116No Hit
ATTTTAATGCATGTGTAAGGAAGGAGTTGAACCAAGACGCATTGAGCAAA30540.14856152169027403No Hit
CTCATAGACTCAGGTACTCCTTCCGTAGAAGGGCCTCTTTTAAGGCCGTG29570.14384296648269165No Hit
CTCCAACTCTATGCTGACAAAATGACTGTCGTCAGCATCCACAGCATTCT28240.1373731949094086No Hit
CTTCTGCATTGTCTCCGAAGAAATAAGATCCTTCGTTACTCATATCAAAA28200.13717861531321965No Hit
ATCTAATGTCGCAGTCTCGCACTCGCGAGATACTGACAAAAACCACAGTG27680.13464908056276312No Hit
AGCAGGTACTGATTCAAAATGGAAGATTTTGTGCGACAATGCTTCAACCC27200.13231412540849555No Hit
ATATGGGATCGAGCACTCTTGAACTGAGAAGCGGGTACTGGGCCATAAGG27190.1322654805094483No Hit
GTATTGGCTTCTAGTGGCCTTGGGGACTAAAGATTGAAATGGTTCAAATT26710.12993052535518074No Hit
GTTCATTGATGCTTAATGCTGGCCCGTATCTTCTGTCTTCTTTACCTATA26650.1296386559608973No Hit
ATTCAGTATTAATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCT26580.1292981416675666No Hit
GCTTAATGCTGGCCCGTATCTTCTGTCTTCTTTACCTATAATGAGGAACC26530.1290549171723304No Hit
ATATACAGGAGAGTCGATGGAAAATGGATGAGGGAACTCGTCCTTTATGA26330.12808201919138557No Hit
TAATTAAGAAGTACACATCGGGGAGACAGGAAAAGAACCCGTCACTTAGG26220.12754692530186593No Hit
CCTCAATAGTATGTCCTGGAAAAGAAGGCAATGGTGAGATTTCGCCAACA26050.12671996201806282No Hit
GAATAAAAGAACTACGGAATCTAATGTCGCAGTCTCGCACTCGCGAGATA25830.12564977423902354No Hit
CAATAGGATGGGAGCTGTAACCACTGAAGTGGCATTTGGCCTGGTGTGTG25500.12404449257046456No Hit
ATAATGAGGAACCCTCTCAAGACGGCGGACTCCACTCCGGATGTGCTTTC25450.12380126807522837No Hit
GTCTAAGAATGTCCACCATCCTTGTTCCGCCAATTTGCGTGCTGTGGCAC25270.12292565989237804No Hit
TTCCTATACAGTTTAACTGCTTTGTCCATGTTATTTGGGTCTCCATTCCC25040.12180682721429148No Hit
GTACGGATAACAAATAGTAGCACTGCTATAACTATTTTAATGCATGTGTA24690.12010425574763804No Hit
CCTATATGAACTACTAGGGAAAAATTTCTCGAACAAGTTGCAGCACTTCT24210.11776930059337047No Hit
TAATAATACATTCCCACGTTGGTCTCGAACTCTCAAAAACCGATCAATGC23270.11319668008292981No Hit
CCTATAATGAGGAACCCTCTCAAGACGGCGGACTCCACTCCGGATGTGCT22970.11173733311151259No Hit
TAGTAGCACTGCTATAACTATTTTAATGCATGTGTAAGGAAGGAGTTGAA22830.11105630452485121No Hit
CCCTTATACTGGAGATCCTCCATACAGCCATGGAACAGGAACAGGGTACA22380.10886728406772536No Hit
CTATTGAGGATGTCAAAAATGCAATTGGGGTCCTCATCGGAGGACTTGAA22070.10735929219726091No Hit
ATATACAATAGGATGGGAGCTGTAACCACTGAAGTGGCATTTGGCCTGGT21890.10648368401441058No Hit
GTACTGGAGTATTCATCCACACCCATTTTGCTGATTCTTATTCCTCTCAT21800.10604587992298539No Hit
GATATTGAGAGGATCAGTTGCTCACAAATCTTGCCTACCTGCCTGTGCAT21390.10405143906204851No Hit
TCTCAAGACGGCGGACTCCACTCCGGATGTGCTTTCATCTGGGTCTTCAA21320.10371092476871784No Hit
TAGTTATTCTGCTGGTGCACTTGCCAGTTGCATGGGCCTCATATACAATA21220.10322447577824542No Hit
ATTAAGAAGTACACATCGGGGAGACAGGAAAAGAACCCGTCACTTAGGAT21180.10302989618205646No Hit
TCCTAGTTCCAGTACTGGTCTAAGAGATGATCTTCTTGAAAATTTGCAGA21080.10254344719158404No Hit
TTCTAACCGAGGTCGAAACGTATGTTCTCTCTATCGTTCCATCAGGCCCC20640.10040307163350544No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACGAGG100.007087741145.025053
AGCGAAA4800.0104.244361
GCTGACC352.7623537E-7103.5742145
GAGCGAA1150.0100.8943561
CGAAAGC6000.083.3894043
TTATATT14350.074.786442
GGGTTAA2150.074.2042851
TATATTC14500.074.012793
GGTCTTT406.663975E-572.501945145
AGGGTGT5300.071.1391756
CAGTACG9500.069.445869
AACTATC554.060721E-665.915676
TATCTTA554.0636533E-665.907659
ATATTCA16300.065.8363954
CAGCATG16500.065.0288859
GGTTAAT2600.061.356752
AATTCTA1200.060.4241645
CTATCTT606.809025E-660.4153448
AGGGTTA1301.8189894E-1255.7747966
TACTGAT6100.054.670947
Produced by FastQC (version 0.11.5)