FastQCFastQC Report
Tue 2 Jul 2019
HWC5JBCX2_l01_n01_SM2_9810_P1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWC5JBCX2_l01_n01_SM2_9810_P1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2157948
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TACCTATACAGGCAGCAATTTCAACAACATTCCCATACACCGGTGTTCCC90530.41951891333804153No Hit
GTGTACAATGATAGATCCAACAAATGGGCCATTACCTGAAGACAATGAGC79570.36872992305653335No Hit
GTTAAAACAGGCCTCAAAAAGATTGCAACATTTAGCGTAGCATTGTTCTT75970.3520474080005635No Hit
CCCATACACCGGTGTTCCCCCTTATTCCCATGGAACGGGAACAGGCTACA74480.34514270038017597No Hit
CTGTTAAAACAGGCCTCAAAAAGATTGCAACATTTAGCGTAGCATTGTTC68150.315809278073429No Hit
GATCTATACTAAATACTGATCAGAGGAACATGATTCTTGAAGAACAATGC65200.30213888379145376No Hit
ACTAAATACTGATCAGAGGAACATGATTCTTGAAGAACAATGCTACGCTA63940.2963000035218643No Hit
GATATACAGAAAGCACTAATTGGTGCCTCTATCTGCTTTTTAAAACCCAA63300.29333422306746965No Hit
TTCTTAATCTATGGGCCATAGCCTCAAGCATGCTATGTTGCCCTACTGGT61370.28439054138468584No Hit
CCTATACAGGCAGCAATTTCAACAACATTCCCATACACCGGTGTTCCCCC58300.2701640632675115No Hit
GTATAGATCTGTTCCTTTTGGTTCTCCAGCTATGAGTTCCAGACACTGCA57450.26622513610151866No Hit
ATCCAATGATGACCAATAACCCCATGGACATCTTCGGAGCTTATGTGTAC55930.25918140752233143No Hit
ACACAATAGACACCGTGATCAGAACACATGAGTACTCGAACAAAGGAAAA51870.2403672377647654No Hit
GTATTTAGTATAGATCTGTTCCTTTTGGTTCTCCAGCTATGAGTTCCAGA49330.2285967965863867No Hit
CAATAGACACCGTGATCAGAACACATGAGTACTCGAACAAAGGAAAACAG48440.22447250814199415No Hit
GTTTTAACAGTGCATCATACAGGAAACCAGTAGGGCAACATAGCATGCTT46660.21622393125320907No Hit
ATATAAATCCGTATTTTCTCTTCATAGATGTACCTATACAGGCAGCAATT45330.2100606687464202No Hit
ATATAGTAGCGCTGAGCTTTCATGGCCTTCTGCTATTTCAAATGCTTCAT45170.20931922363282154No Hit
GTAAGAAATACAGTAAAAATTGAATTTAATGCGATTCTTCTACCTCCAAA43920.20352668368283203No Hit
ATACTAAATACTGATCAGAGGAACATGATTCTTGAAGAACAATGCTACGC42910.1988463114032405No Hit
CATGTATCCAATGATGACCAATAACCCCATGGACATCTTCGGAGCTTATG41550.19254402793765188No Hit
TCATAGATGTACCTATACAGGCAGCAATTTCAACAACATTCCCATACACC41470.19217330538085253No Hit
CCAATAACCCCATGGACATCTTCGGAGCTTATGTGTACCCAATCTCACCA40120.18591736223486385No Hit
GCCTTGGAATGGATAAAAAACAAAAGATGCTTAACTGATATACAGAAAGC39530.18318328337846881No Hit
ATCTAATGATGACCAATAACCCCATGGACATCTTCGGAGCTTATGTGTAC39530.18318328337846881No Hit
CTATAGAACATGCTTGTAAATTCAAACATTCCAGTTTCGTTACAGTAACT38670.179198015892876No Hit
CTACTATATTGTCTCATGGTCATGTACCTGAATCCTGGAAATTATTCAAT37950.17586151288168203No Hit
GTACAATGATAGATCCAACAAATGGGCCATTACCTGAAGACAATGAGCCA36840.17071773740609134No Hit
CATTATAGGTATTTCTTCACAAGAGCTGAATTTCCCATAGAGCTCTGCTT36410.16872510366329493No Hit
GCTTTAGCACTTCCATTACATCCTTTGCAATTCCTTCCCCATTTTTCTGA35630.16511055873450148No Hit
CCCTTATTCCCATGGAACGGGAACAGGCTACACAATAGACACCGTGATCA35460.1643227733013029No Hit
TCTGCATACTGTCCAATCGAAAGTCTGTCTCCCCTGGGTTAATTTGTCTA34800.16126431220770843No Hit
CAATAACCCCATGGACATCTTCGGAGCTTATGTGTACCCAATCTCACCAA34010.15760342695931506No Hit
GTAGAAACACGAGCCTTTTTTCATTTTAATCATTTGTTTATCACATGTAT33440.1549620287421198No Hit
CCCCATGGACATCTTCGGAGCTTATGTGTACCCAATCTCACCAAGGTGAG32890.15241331116412443No Hit
GTGTGAATGTGATGCTTGTTTTTCGCACAAAGCACAGAGTGTTCCTAGTT32250.1494475307097298No Hit
GCCTCAAGCATGCTATGTTGCCCTACTGGTTTCCTGTATGATGCACTGTT31660.14671345185333476No Hit
CTTCATAGATGTACCTATACAGGCAGCAATTTCAACAACATTCCCATACA31510.146018347059336No Hit
GAACACATGAGTACTCGAACAAAGGAAAACAGTATGTTTCTGACATCACA31490.14592566642013616No Hit
CCTGAAGACAATGAGCCAAGTGCCTATGCACAATTAGATTGCGTTCTGGA31440.1456939648221366No Hit
GTATGATGCACTGTTAAAACAGGCCTCAAAAAGATTGCAACATTTAGCGT31060.14393303267733978No Hit
ATTTAGTATAGATCTGTTCCTTTTGGTTCTCCAGCTATGAGTTCCAGACA30720.1423574618109426No Hit
GTTCATAGCTGAGACCATCTGCATTTCCCGTCTCACTCCTGGCACTGAGG30530.14147699573854422No Hit
ACTATATTGTCTCATGGTCATGTACCTGAATCCTGGAAATTATTCAATGC30210.1399941055113469No Hit
GTATGCAGAAACCAGCCTGCTGCAACGGCACTAAACACAACAATAACCTC29970.1388819378409489No Hit
GCTTATGTGTACCCAATCTCACCAAGGTGAGCCATTGCTTTTTCAAAATC29270.13563811546895477No Hit
ATATTGTCTCATGGTCATGTACCTGAATCCTGGAAATTATTCAATGCAAG28810.13350646076735861No Hit
CACCAAGGTGAGCCATTGCTTTTTCAAAATCATCCTTTGACATTCTTCCT28510.13211625117936113No Hit
TTCCCATACACCGGTGTTCCCCCTTATTCCCATGGAACGGGAACAGGCTA27930.129428512642566No Hit
CATTCACACAGAGCTCATAGCAGAGCAGCGAGATCCTCAGTGCCAGGAGT27770.12868706752896733No Hit
CAATTAGATTGCGTTCTGGAGGCTTTGGATAGAATGGATGAGGAACATCC27250.1262773709097717No Hit
GCTATGAGCTCTGTGTGAATGTGATGCTTGTTTTTCGCACAAAGCACAGA26930.12479448068257437No Hit
GTTTATCACATGTATCCAATGATGACCAATAACCCCATGGACATCTTCGG26920.12474814036297446No Hit
GTCCTATTGTCCAAGCCATGAAATGGAGAGCTGATAAAATAAAAGAACAA26260.12168967926937999No Hit
TTCATAATCTAGTCGTGCATCCATTCTTAATCTATGGGCCATAGCCTCAA26050.12071653255778174No Hit
ATGATAGATCCAACAAATGGGCCATTACCTGAAGACAATGAGCCAAGTGC25000.11585079899979055No Hit
ATATAACATAATAAGAAAATTCAATACATCAAGAATTGAAAAGAACCCTT24850.1151556942057918No Hit
GTATAGTATGGTTATCCAATCCATCATCATTTAAAGATGCAGCAGTAATG24680.11436790877259323No Hit
ATCTAGTCGTGCATCCATTCTTAATCTATGGGCCATAGCCTCAAGCATGC24580.11390450557659407No Hit
GTATAGATCTGTTCCTTTTGGTTCTCCACTGTCCAATCGAAAGTCTGTCT24390.11302403950419566No Hit
ATGTTGCCCTACTGGTTTCCTGTATGATGCACTGTTAAAACAGGCCTCAA24180.11205089279259742No Hit
ATGATGCACTGTTAAAACAGGCCTCAAAAAGATTGCAACATTTAGCGTAG24090.11163382991619816No Hit
ATACTGATCAGAGGAACATGATTCTTGAAGAACAATGCTACGCTAAATGT24000.11121676703979891No Hit
CTGTGAGGCTGCTTGAAACAGACCTGGATGTTCCTCATCCATTCTATCCA23830.11042898160660035No Hit
GTTCCAGACACTGCATCGTAATCCATTTTCCTCACAGGACCATGTGCTGG23790.11024362032820068No Hit
GTTCTATAGAGATGGATTTGTATCTAACTTTGCAATGGAAATTCCTTCAT23740.11001191873020111No Hit
GCACAGAGTGTTCCTAGTTTTACTTGCATTGAATAATTTCCAGGATTCAG23670.1096875364930017No Hit
GGCTACACAATAGACACCGTGATCAGAACACATGAGTACTCGAACAAAGG23530.10903877201860286No Hit
GAATGGATGAGGAACATCCAGGTCTGTTTCAAGCAGCCTCACAGAATGCC23520.10899243169900294No Hit
GAATAAAGGGGCCAAATAAAGAGACAATAAACAGAGAGGTATCAATTTTG23190.1074632011522057No Hit
AAATTACACTGTTGGTTCGGTGGGAAAGAATTTGACCTAGACTCTGCCTT22710.10523886581140973No Hit
TATGTGTACCCAATCTCACCAAGGTGAGCCATTGCTTTTTCAAAATCATC22430.10394133686261209No Hit
GATGTACCTATACAGGCAGCAATTTCAACAACATTCCCATACACCGGTGT22280.10324623206861333No Hit
ACCTAATGGACCCTGAATACAAAGGGCGGTTACTTCACCCTCAAAATCCC22260.10315355142941349No Hit
ATTAGATTGCGTTCTGGAGGCTTTGGATAGAATGGATGAGGAACATCCAG22040.10213406439821533No Hit
ATATTACTCTTAGTGCTTTTCCAAAGGATCCTTTTACTAGTTTCCCTTCT21840.10120725800621701No Hit
GTCTGGAACTCATAGCTGGAGAACCAAAAGGAACAGATCTATACTAAATA21650.1003267919338186No Hit
GCTATGAACACAGCAAAAACAATGAATGGAATGGGGAAAGGAGAAGACGT21610.10014143065541896No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCAGA1900.0118.329381
GAAACAC9350.0110.121124
AAACACG9600.0108.008695
AACACGT3250.0100.39526
AACACGA7500.095.71016
ACACGTG3450.094.564247
ACACGAG7750.092.611947
TAGAAAC11850.092.400132
ACGAGCC6800.087.425569
CGGAGCG750.087.011195
CACGAGC8300.085.601528
ACAACGC2900.084.9991158
GCACGCA1650.083.499462
GGAGCGT703.092282E-1182.865886
CAACGCA3000.082.165829
AGCACGC1700.081.056741
CACGTGC4300.074.185288
AAGCGGA2600.072.512692
ACGTGCC3150.071.3484659
CACGCAC1950.070.653393
Produced by FastQC (version 0.11.5)