FastQCFastQC Report
Thu 11 Jul 2019
HWC5JBCX2_l01_n01_SM2_9810_P1_DM2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWC5JBCX2_l01_n01_SM2_9810_P1_DM2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2164973
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TACCTATACAGGCAGCAATTTCAACAACATTCCCATACACCGGTGTTCCC90750.41917381879589255No Hit
GTGTACAATGATAGATCCAACAAATGGGCCATTACCTGAAGACAATGAGC79810.36864201077796355No Hit
GTTAAAACAGGCCTCAAAAAGATTGCAACATTTAGCGTAGCATTGTTCTT76270.3522907675984874No Hit
CCCATACACCGGTGTTCCCCCTTATTCCCATGGAACGGGAACAGGCTACA74670.34490037520098404No Hit
CTGTTAAAACAGGCCTCAAAAAGATTGCAACATTTAGCGTAGCATTGTTC68440.31612403480320544No Hit
GATCTATACTAAATACTGATCAGAGGAACATGATTCTTGAAGAACAATGC65380.30198990934298026No Hit
ACTAAATACTGATCAGAGGAACATGATTCTTGAAGAACAATGCTACGCTA64090.2960314054724932No Hit
GATATACAGAAAGCACTAATTGGTGCCTCTATCTGCTTTTTAAAACCCAA63420.2929366786560387No Hit
TTCTTAATCTATGGGCCATAGCCTCAAGCATGCTATGTTGCCCTACTGGT61620.2846224872088474No Hit
CCTATACAGGCAGCAATTTCAACAACATTCCCATACACCGGTGTTCCCCC58400.26974932250887196No Hit
GTATAGATCTGTTCCTTTTGGTTCTCCAGCTATGAGTTCCAGACACTGCA57580.26596174640515147No Hit
ATCCAATGATGACCAATAACCCCATGGACATCTTCGGAGCTTATGTGTAC56110.2591718233899453No Hit
ACACAATAGACACCGTGATCAGAACACATGAGTACTCGAACAAAGGAAAA52060.24046489263376494No Hit
GTATTTAGTATAGATCTGTTCCTTTTGGTTCTCCAGCTATGAGTTCCAGA49490.22859407484527522No Hit
CAATAGACACCGTGATCAGAACACATGAGTACTCGAACAAAGGAAAACAG48630.2246217389316172No Hit
GTTTTAACAGTGCATCATACAGGAAACCAGTAGGGCAACATAGCATGCTT46810.2162151675794571No Hit
ATATAAATCCGTATTTTCTCTTCATAGATGTACCTATACAGGCAGCAATT45440.20988714408909487No Hit
ATATAGTAGCGCTGAGCTTTCATGGCCTTCTGCTATTTCAAATGCTTCAT45330.20937905461176654No Hit
GTAAGAAATACAGTAAAAATTGAATTTAATGCGATTCTTCTACCTCCAAA44090.20365150050370143No Hit
ATACTAAATACTGATCAGAGGAACATGATTCTTGAAGAACAATGCTACGC43000.19861679568290228No Hit
CATGTATCCAATGATGACCAATAACCCCATGGACATCTTCGGAGCTTATG41690.19256591190744643No Hit
TCATAGATGTACCTATACAGGCAGCAATTTCAACAACATTCCCATACACC41610.19219639228757127No Hit
CCAATAACCCCATGGACATCTTCGGAGCTTATGTGTACCCAATCTCACCA40200.18568360898727143No Hit
ATCTAATGATGACCAATAACCCCATGGACATCTTCGGAGCTTATGTGTAC39660.18318935155311405No Hit
GCCTTGGAATGGATAAAAAACAAAAGATGCTTAACTGATATACAGAAAGC39590.1828660218857233No Hit
CTATAGAACATGCTTGTAAATTCAAACATTCCAGTTTCGTTACAGTAACT38770.17907844578200283No Hit
CTACTATATTGTCTCATGGTCATGTACCTGAATCCTGGAAATTATTCAAT38060.17579895915561072No Hit
GTACAATGATAGATCCAACAAATGGGCCATTACCTGAAGACAATGAGCCA36930.17057949452487398No Hit
CATTATAGGTATTTCTTCACAAGAGCTGAATTTCCCATAGAGCTCTGCTT36560.16887046628295133No Hit
GCTTTAGCACTTCCATTACATCCTTTGCAATTCCTTCCCCATTTTTCTGA35820.16545240979910605No Hit
CCCTTATTCCCATGGAACGGGAACAGGCTACACAATAGACACCGTGATCA35490.16392814136712097No Hit
TCTGCATACTGTCCAATCGAAAGTCTGTCTCCCCTGGGTTAATTTGTCTA34890.16115674421805723No Hit
CAATAACCCCATGGACATCTTCGGAGCTTATGTGTACCCAATCTCACCAA34080.15741535806682116No Hit
GTAGAAACACGAGCCTTTTTTCATTTTAATCATTTGTTTATCACATGTAT33560.15501348053763256No Hit
CCCCATGGACATCTTCGGAGCTTATGTGTACCCAATCTCACCAAGGTGAG33020.1525192231034752No Hit
GTGTGAATGTGATGCTTGTTTTTCGCACAAAGCACAGAGTGTTCCTAGTT32350.14942449628702067No Hit
GCCTCAAGCATGCTATGTTGCCCTACTGGTTTCCTGTATGATGCACTGTT31740.1466069091854725No Hit
CTTCATAGATGTACCTATACAGGCAGCAATTTCAACAACATTCCCATACA31630.14609881970814417No Hit
GAACACATGAGTACTCGAACAAAGGAAAACAGTATGTTTCTGACATCACA31560.1457754900407534No Hit
CCTGAAGACAATGAGCCAAGTGCCTATGCACAATTAGATTGCGTTCTGGA31540.14568311013578458No Hit
GTATGATGCACTGTTAAAACAGGCCTCAAAAAGATTGCAACATTTAGCGT31150.14388170198889316No Hit
ATTTAGTATAGATCTGTTCCTTTTGGTTCTCCAGCTATGAGTTCCAGACA30870.14258838331933008No Hit
GTTCATAGCTGAGACCATCTGCATTTCCCGTCTCACTCCTGGCACTGAGG30630.14147982445970458No Hit
ACTATATTGTCTCATGGTCATGTACCTGAATCCTGGAAATTATTCAATGC30300.1399555560277195No Hit
GTATGCAGAAACCAGCCTGCTGCAACGGCACTAAACACAACAATAACCTC30050.1388008072156096No Hit
GCTTATGTGTACCCAATCTCACCAAGGTGAGCCATTGCTTTTTCAAAATC29300.1353365607792799No Hit
ATATTGTCTCATGGTCATGTACCTGAATCCTGGAAATTATTCAATGCAAG28930.13362753253735726No Hit
CACCAAGGTGAGCCATTGCTTTTTCAAAATCATCCTTTGACATTCTTCCT28640.1322880239153098No Hit
TTCCCATACACCGGTGTTCCCCCTTATTCCCATGGAACGGGAACAGGCTA28050.12956281671873043No Hit
CATTCACACAGAGCTCATAGCAGAGCAGCGAGATCCTCAGTGCCAGGAGT27880.1287775875264957No Hit
CAATTAGATTGCGTTCTGGAGGCTTTGGATAGAATGGATGAGGAACATCC27310.12614476023488516No Hit
GTTTATCACATGTATCCAATGATGACCAATAACCCCATGGACATCTTCGG27000.12471287170786886No Hit
GCTATGAGCTCTGTGTGAATGTGATGCTTGTTTTTCGCACAAAGCACAGA26960.1245281118979313No Hit
GTCCTATTGTCCAAGCCATGAAATGGAGAGCTGATAAAATAAAAGAACAA26350.12171052479638314No Hit
TTCATAATCTAGTCGTGCATCCATTCTTAATCTATGGGCCATAGCCTCAA26150.12078672574669523No Hit
ATGATAGATCCAACAAATGGGCCATTACCTGAAGACAATGAGCCAAGTGC25130.11607535059328683No Hit
ATATAACATAATAAGAAAATTCAATACATCAAGAATTGAAAAGAACCCTT24920.11510536159111454No Hit
GTATAGTATGGTTATCCAATCCATCATCATTTAAAGATGCAGCAGTAATG24790.11450489220881739No Hit
ATCTAGTCGTGCATCCATTCTTAATCTATGGGCCATAGCCTCAAGCATGC24650.11385823287403585No Hit
GTATAGATCTGTTCCTTTTGGTTCTCCACTGTCCAATCGAAAGTCTGTCT24450.11293443382434792No Hit
ATGTTGCCCTACTGGTTTCCTGTATGATGCACTGTTAAAACAGGCCTCAA24330.11238015439453516No Hit
ATGATGCACTGTTAAAACAGGCCTCAAAAAGATTGCAACATTTAGCGTAG24140.11150254529733165No Hit
ATACTGATCAGAGGAACATGATTCTTGAAGAACAATGCTACGCTAAATGT24040.11104064577248769No Hit
CTGTGAGGCTGCTTGAAACAGACCTGGATGTTCCTCATCCATTCTATCCA23930.11053255629515935No Hit
GTTCCAGACACTGCATCGTAATCCATTTTCCTCACAGGACCATGTGCTGG23870.11025541658025297No Hit
GTTCTATAGAGATGGATTTGTATCTAACTTTGCAATGGAAATTCCTTCAT23800.10993208691286219No Hit
GCACAGAGTGTTCCTAGTTTTACTTGCATTGAATAATTTCCAGGATTCAG23710.10951637734050264No Hit
GAATGGATGAGGAACATCCAGGTCTGTTTCAAGCAGCCTCACAGAATGCC23610.10905447781565866No Hit
GGCTACACAATAGACACCGTGATCAGAACACATGAGTACTCGAACAAAGG23580.10891590795820548No Hit
GAATAAAGGGGCCAAATAAAGAGACAATAAACAGAGAGGTATCAATTTTG23250.10739163952622041No Hit
AAATTACACTGTTGGTTCGGTGGGAAAGAATTTGACCTAGACTCTGCCTT22730.10498976199703183No Hit
TATGTGTACCCAATCTCACCAAGGTGAGCCATTGCTTTTTCAAAATCATC22500.10392739308989073No Hit
ACCTAATGGACCCTGAATACAAAGGGCGGTTACTTCACCCTCAAAATCCC22370.10332692370759358No Hit
GATGTACCTATACAGGCAGCAATTTCAACAACATTCCCATACACCGGTGT22350.1032345438026248No Hit
ATTAGATTGCGTTCTGGAGGCTTTGGATAGAATGGATGAGGAACATCCAG22110.10212598494299929No Hit
ATATTACTCTTAGTGCTTTTCCAAAGGATCCTTTTACTAGTTTCCCTTCT21890.10110980598834259No Hit
GTCTGGAACTCATAGCTGGAGAACCAAAAGGAACAGATCTATACTAAATA21740.10041695670107663No Hit
GCTATGAACACAGCAAAAACAATGAATGGAATGGGGAAAGGAGAAGACGT21700.10023219689113906No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCAGA1500.0140.199771
GAAACAC8600.0106.2337344
ACAACGC2400.0102.706488
AGCACGC2550.0102.377111
AAACACG9050.0100.95145
CAACGCA2500.098.598239
GCACGCA2600.097.607922
AACACGT3450.094.574436
ACACGTG3600.090.623377
ACGTGCA1050.089.7602849
AACACGA6550.089.665236
GAGCGTT651.6370905E-1189.2291647
ACACGAG6600.088.975677
TAGAAAC11300.087.267162
CACGCAC2950.086.027333
CACGAGC7150.081.1174248
CGGAGCG554.3932232E-879.100455
TAGTAGA1600.077.0405353
CACGTGC4250.076.763328
ACGAGCC5600.076.382559
Produced by FastQC (version 0.11.5)