FastQCFastQC Report
Tue 2 Jul 2019
HWC5JBCX2_l01_n01_SM2_9732_P1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWC5JBCX2_l01_n01_SM2_9732_P1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2203992
Sequences flagged as poor quality0
Sequence length151
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTATAGAACATGCTTGTAAATTCAAACATTCCAGTTTCGTTACAGTAACT79640.3613443242988178No Hit
GTAAGAAATACAGTAAAATTGAATTTAATGCGATTCTTCTACCTCCAAAA74410.3376146555885865No Hit
GATATACAGAAAGCACTAATTGGTGCCTCTATCTGCTTTTTAAAACCCAA70950.32191586902311803No Hit
AGCACGCACTTTCTTAAAATGTCGCTGTTTGGAGACACAATTGCCTACCT66370.3011353943208505No Hit
ATATAGTAGCGCTGAGCTTTCATGGCCTTCTGCTATTTCAAATGCTTCAT54300.2463711302037394No Hit
CCTATAATGTTTGAACCATTCCAGATTCTTTCAATTTGTTCTTTTATTTT52850.2397921589552049No Hit
GTTCTATAGAGATGGATTTGTATCTAACTTTGCAATGGAAATTCCTTCAT50960.23121681022435656No Hit
CTACTATATTGTCTCATGGTCATGTACCTGAATCCTGGAAATTATTCAAT48480.21996450077858723No Hit
ATTACTATGTGGTCACTCAATACTTCCATGTTGTCAGAGAGTACTTCCTT46240.20980112450498914No Hit
GCCTTGGAATGGATAAAAAACAAAAGATGCTTAACTGATATACAGAAAGC44310.2010442869121122No Hit
ACTATATTGTCTCATGGTCATGTACCTGAATCCTGGAAATTATTCAATGC40120.18203332861462293No Hit
GTGTGAATGTGATGCTTGTTTTTCGCACAAAGCACAGAGCGTTCCTAGCT38230.17345797988377454No Hit
CATTATAGGTATTTCTTCACAAGAGCTGAATTTCCCATAGAGCTCTGCTT38050.17264128000464612No Hit
GCTTTAGCACTTCCATTACATCCTTTGCAATTCCTTCCCCATTCTTCTGA37430.1698282026432038No Hit
ATATTGTCTCATGGTCATGTACCTGAATCCTGGAAATTATTCAATGCAAG36650.1662891698336473No Hit
CATTCACACAGAGCTCATAGCAGAGCAGCGAGATCCTCAGTGCCAGGAGT34860.15816754325787027No Hit
ATATTACTCTTAGTGCTTTTCCAAAGGATCCTTTTACTAGTTTCCCTTCT33870.15367569392266398No Hit
GTATTTACACAGTCTCTCCAAAGTTTCCATAGGAAATTTTCGTTAGCTTT33590.1524052718884642No Hit
GTTCATAGCTGAGACCATTTGCATTTCCCGTCTCACTCCTGGCACTGAGG33190.15059038326817883No Hit
CAATTACTATGTGGTCACTCAATACTTCCATGTTGTCAGAGAGTACTTCC32970.14959219452702188No Hit
TTCTAATGGTATGCTGAACAGATCAGGACAAGGTATTTGAGCCTTTAGTC32200.14609853393297254No Hit
GCTATGAACACAGCAAAAACAATGAATGGAATGGGAAAAGGAGAAGACGT31480.14283173441645886No Hit
GCTATGAGCTCTGTGTGAATGTGATGCTTGTTTTTCGCACAAAGCACAGA31240.14174280124428765No Hit
GTTTATTGTCTCTTTATTTGGCCCCTTTATTCTTATTTTCATGTTTGCTC31110.14115296244269487No Hit
GTATAGATCTGTTCCTTTTGGTTCTCCAACTATGAGTTCCAGACACTGCA31090.14106221801168062No Hit
TACCTATACAGGCAGCAATTTCAACAACATTCCCATACACCGGTGTTCCC30710.1393380738224095No Hit
AGTAGAAACAACGCACTTTTTCCAGTTTATTTGCTGACATTGATTACAAT28250.12817650880765447No Hit
GAACTAATGATATTCAGCTACAATCAAGACTATTCGTTAAGTAATGAATC28220.12804039216113308No Hit
CCATTAGAAAGATATAATGAAGAAACAAGGGCGAAATTAAAAAGGCTGAA28120.12758667000606172No Hit
GTATAGTATGGTTATCCAATCCATCATCATTTAAAGATGCAGCAGTAATG27220.12350317061041963No Hit
GCTCATAGCAGAGCAGCGAGATCCTCAGTGCCAGGAGTGAGACGGGAAAT27170.12327630953288396No Hit
ATGCTACACTGCTTACAAGGTGAAAATTGGAGGCAAATATATCACCCGGG26500.12023637109390597No Hit
GTATAGCACCTGTCTTGTTCTCCAACAAAATAGCCAGATTAGGGAAAGGA26290.11928355456825614No Hit
GTGTAAATACAATAAGTAATGAGGAAATGAGTAACGAGTTACAAAAAACC25910.11755941037898505No Hit
GTATTTAGTATAGATCTGTTCCTTTTGGTTCTCCAACTATGAGTTCCAGA25770.11692419936188515No Hit
GTTAAAACAGGCCTCAAAAAGATTGCAACATTTAGCGTAGCATTGTTCTT24810.11256846667320025No Hit
AAATTACACTGTTGGTTCGGTGGGAAAGAATTTGACCTAGACTCTGCCTT24720.11216011673363606No Hit
GTACTTCCTTCATTGCTTCTTTAGCCTGAATTTCTTTTTGGTAACTGTGT24680.11197862787160752No Hit
GTCATGTACCTGAATCCTGGAAATTATTCAATGCAAGTAAAGCTAGGAAC24660.11188788344059326No Hit
CTGATATACAGAAAGCACTAATTGGTGCCTCTATCTGCTTTTTAAAACCC24370.11057208919088635No Hit
GATATATCCCTTAGTCTAGATATTGTTTGTCCAAGTCTAAAGTCAATTCC24340.11043597254436495No Hit
ATACTAAATGGAATGAATGCTTAAATCCACGAATCTTTTTGGCTATGACT24330.11039060032885782No Hit
GTCTCATGGTCATGTACCTGAATCCTGGAAATTATTCAATGCAAGTAAAG23920.10853033949306531No Hit
GTTTAATATGCTATCTACCGTGTTGGGAGTAGCAGCACTAGGCATCAAAA23880.10834885063103679No Hit
TCCCATAAGTATTCCTTGTTTCCAATGTTTTTGATGCCTAGTGCTGCTAC23860.1082581062000225No Hit
CTTCTGACTTGCCCCAAGGGATCTCAATACTCCAATGTTGCTTTGCAGTT23830.1081219895535011No Hit
GATTATAGGTACACATACAAATGCCACAGAGGAGATTCCAAAGTGGAAGG23500.10662470644176568No Hit
GACCTAGACTCTGCCTTGGAATGGATAAAAAACAAAAGATGCTTAACTGA23420.1062617287177086No Hit
CAATACTTCCATGTTGTCAGAGAGTACTTCCTTCATTGCTTCTTTAGCCT23270.10558114548510159No Hit
GTCCTATTGTCCAAGCCATGAAATGGAGAGCTGATAAAATAAAAGAACAA23040.10453758452843749No Hit
ATTACATCCTTTGCAATTCCTTCCCCATTCTTCTGACTTGCCCCAAGGGA22770.10331253470974486No Hit
GGATAGAGACTCCCAAGTATCTGGCTGATTTGTTTGATTATAAGACCAAA22490.10204211267554511No Hit
GTTTGAACCATTCCAGATTCTTTCAATTTGTTCTTTTATTTTATCAGCTC22430.10176987938250229No Hit
ATCCAATGATGACCAATAACCCCATGGACATCTTCGGAGCTTATGTGTAC22230.1008624350723596No Hit
GTCCTGATCTGTTCAGCATACCATTAGAAAGATATAATGAAGAAACAAGG22200.1007263184258382No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCCTCC100.007091672144.9984145
TGTGCCC100.007091672144.9984145
GAGCAGA1900.0129.782521
AGCACGC7650.0122.2979351
CACGCAC7750.0120.708943
GCACGCA7750.0120.708942
GAAACAC5250.0114.646394
AACACGT1350.0112.799556
AAACACG5350.0112.503465
GAGCACG203.861271E-4108.7882841
ACACGTG1450.0104.998837
ACAACGC4850.091.1845558
AACACGA5050.089.0270846
CGGTGCA502.2659151E-887.022722
CAACGCA5150.085.872839
AACAACG5200.085.0471347
ACACGAG5350.084.017767
ACGTGCA900.080.554669
GGTGCAT554.3888576E-879.111573
ACGAGCC3300.079.0900349
Produced by FastQC (version 0.11.5)