FastQCFastQC Report
Tue 2 Jul 2019
HWC5JBCX2_l01_n01_SM2_9730_P1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWC5JBCX2_l01_n01_SM2_9730_P1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1994091
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAAGAAATACAGTAAAATTGAATTTAATGCGATTCTTCTACTTCCAAAA72220.36217003135764614No Hit
CTATAGAACATGCTTGTAAATTCAAACATTCCAGTTTCGTTACAGTAACT69300.34752676783557024No Hit
GATATACAGAAAGCACTAATTGGTGCCTCTATCTGCTTTTTAAAACCCAA57260.2871483798883802No Hit
AGCACGCACTTTCTTAAAATGTCGCTGTTTGGAGACACAATTGCCTACCT47380.23760199509450672No Hit
ATATAGTAGCGCTGAGCTTTCATGGCCTTCTGCTATTTCAAATGCTTCAT43770.2194985083428991No Hit
CTACTATATTGTCTCATGGTCATGTACCTGAATCCTGGAAATTATTCAAT42590.2135810251387725No Hit
CATTATAGGTATTTCTTCACAAGAGCTGAATTTCCCATAGAGCTCTGCTT40010.20064279915008895No Hit
CCTATAATGTTTGAACCATTCCAGATTCTTTCAATTTGTTCTTTTATTTT39130.19622976082836743No Hit
GTTCTATAGAGATGGATTTGTATCTAACTTTGCAATGGAGATTCCTTCAT38350.19231820413411424No Hit
ACTATATTGTCTCATGGTCATGTACCTGAATCCTGGAAATTATTCAATGC35780.1794301263081775No Hit
GCCTTGGAATGGATAAAAAACAAAAGATGCTTAACTGATATACAGAAAGC34100.17100523496670914No Hit
GCTTTAGCACTTCCATTACATCCTTTGCAATTCCTTCCCCATTCTTCTGA33430.16764530806267114No Hit
ATATTGTCTCATGGTCATGTACCTGAATCCTGGAAATTATTCAATGCAAG32310.1620287138350256No Hit
GAATAAAGGGGCCAAATAAAGAGACAATAAACAGAGAGGTATCAATTTTG31590.15841804611725344No Hit
CATTCACACAGAGCTCATAGCAGAGCAGCGAGATCCTCAGTGCCAGGAGT30540.1531524890288357No Hit
GTTCATAGCTGAGACCATCTGCATTTCCCGTCTCACTCCTGGCACTGAGG30020.15054478456600026No Hit
ATTACTATGTGGTCACTCAATACTTCCATGTTGTCAGAGAGTACTTCCTT28820.14452700503638No Hit
GTATAGTATGGTTATCCAATCCATCATCATTTAAAGATGCAGCAGTAATG28590.14337359729320276No Hit
GTGTGAATGTGATGCTTGTTTTTCGCACAAAGCACAGAGTGTTCCTAGTT28570.1432733009677091No Hit
TTCTAATGGTATGCTGAACAGATCAGGACAAGGTATTTGAGCCTTTAGTC26160.13118759374572175No Hit
AGTAGAAACAACGCACTTTTTCCAGTTTATTTGCTGACATTGATTACAAT26100.13088670476924072No Hit
TTATTATTGTCATTCCTATTGCCATATCTGCTGATTCATTTACTCCAGCA26060.13068611211825337No Hit
GCTATGAACACAGCAAAAACAATGAATGGAATGGGAAAAGGAGAAGACGT25630.12852974112013946No Hit
GTCCTATTGTCCAAGCCATGAAATGGAGAGCTGATAAAATAAAAGAACAA25010.12542055502983565No Hit
GCTATGAGCTCTGTGTGAATGTGATGCTTGTTTTTCGCACAAAGCACAGA24570.12321403586897489No Hit
GTTTAATATGCTATCTACCGTGTTGGGAGTAGCAGCACTAGGCATCAAAA23350.11709596001386094No Hit
CTTCTGACTTGCCCCAAGGGATCTCAATACTCCAATGTTGCTTTGCAGTT23210.11639388573540525No Hit
GTATAGATCTGTTCCTTTTGGTTCTCCAACTGTGAGTTCCAGACACTGCA22740.11403692208630399No Hit
CCATTAGAAAGATATAATGAAGAAACAAGGGCGAAATTAAAAAGGCTGAA22480.11273306985488626No Hit
GTCATGTACCTGAATCCTGGAAATTATTCAATGCAAGTAAAACTAGGAAC22280.11173010659994956No Hit
CAATTACTATGTGGTCACTCAATACTTCCATGTTGTCAGAGAGTACTTCC22000.11032595804303816No Hit
ATCCAATGATGACCAATAACCCCATGGACATCTTCGGAGCTTATGTGTAC21880.10972418009007615No Hit
GCTCATAGCAGAGCAGCGAGATCCTCAGTGCCAGGAGTGAGACGGGAAAT21870.1096740319273293No Hit
GTCTCATGGTCATGTACCTGAATCCTGGAAATTATTCAATGCAAGTAAAA21350.10706632746449385No Hit
GTTTATTGTCTCTTTATTTGGCCCCTTTATTCTTATTTTCATGTTTACTC21050.10556188258208878No Hit
CTATATGTGTCAGCCTCATTGTCATACTTACTATATTCGGATATATTGCT20850.10455891932715207No Hit
CTGATATACAGAAAGCACTAATTGGTGCCTCTATCTGCTTTTTAAAACCC20810.10435832667616474No Hit
ATACTAAATGGAATGAATGCTTAAATCCACGAATCTTTTTGGCTATGACT20570.10315477077024067No Hit
GCACTAATTGGTGCCTCTATCTGCTTTTTAAAACCCAAAGACCAGGAAAG20330.10195121486431663No Hit
GTATATGCTTCTCCATATTTTATTTTGAGCAATGCATTACTGTCTGGGCC20040.1004969181446584No Hit
ATTACATCCTTTGCAATTCCTTCCCCATTCTTCTGACTTGCCCCAAGGGA19950.10004558467993688No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGTGCA351.902663E-9124.294582
AGCACGC5700.0123.413831
CACGCAC5800.0121.2586443
GCACGCA5800.0121.2586442
GAGCAGA1150.0119.8181841
GAAACAC4550.0109.9501344
ACGTGCA600.0108.749579
AAACACG4700.0106.4410865
AACACGT1300.0100.3867346
ACAACGC4850.091.18528
CAACGCA4950.090.8077249
GGCCTAC259.3770586E-487.025831
GGTGCAT502.2686436E-887.0061953
ACACGTG1500.086.999657
ACGGACC259.388275E-486.99965145
AGAAGCG900.080.559275
AACACGA4650.079.517816
AACAACG5750.078.17367
ACACGAG4950.074.696687
GAAGCGG1950.074.381061
Produced by FastQC (version 0.11.5)