FastQCFastQC Report
Thu 11 Jul 2019
HWC5JBCX2_l01_n01_SM2_9693_P1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWC5JBCX2_l01_n01_SM2_9693_P1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences286119
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATATACAGAAAGCACTAATTGGTGCCTCTATCTGCTTTTTAAAACCCAA12520.43758016769246366No Hit
CTATAGAACATGCTTGTAAATTCAAACATTCCAGTTTCGTTACAGTAACT11600.4054257144754455No Hit
ATATAGTAGCGCTGAGCTTTCATGGCCTTCTGCTATTTCAAATGCTTCAT9480.33133067010579514No Hit
GCCTTGGAATGGATAAAAAACAAAAGATGCTTAACTGATATACAGAAAGC7020.24535245824289895No Hit
GTGTGAATGTGATGCTTGTTTTTCGCACAAAGCACAGAGTGTTCCTAGTT6300.22018810355131954No Hit
GTAAGAAATACAGTAAAAATTGAATTTAATGCGATTCTTCTACCTCCAAA6290.2198385986250476No Hit
GTATAGATCTGTTCCTTTTGGTTCTCCAGCTATGAGTTCCAGACACTGCA5120.17894652225123114No Hit
GCTATGAGCTCTGTGTGAATGTGATGCTTGTTTTTCGCACAAAGCACAGA5070.17719899761987146No Hit
TTCTAATGGTATGCTGAACAGATCAGGACAAGGTATTTGAGCCTTTAGTC5030.1758009779147837No Hit
GCACAGAGTGTTCCTAGTTTTACTTGCATTGAATAATTTCCAGGATTCAG4980.17405345328342403No Hit
GTTAAAACAGGCCTCAAAAAGATTGCAACATTTAGCGTAGCATTGTTCTT4830.168810879389345No Hit
GTTCTATAGAGATGGATTTGTATCTAACTTTGCAATGGAAATTCCTTCAT4810.1681118695368011No Hit
CATTATAGGTATTTCTTCACAAGAGCTGAATTTCCCATAGAGCTCTGCTT4770.16671384983171336No Hit
ATATTACTCTTAGTGCTTTTCCAAAGGATCCTTTTACTAGTTTCCCTTCT4590.16042276115881854No Hit
CTACTATATTGTCTCATGGTCATGTACCTGAATCCTGGAAATTATTCAAT4540.15867523652745885No Hit
TACCTATACAGGCAGCAATTTCAACAACATTCCCATACACCGGTGTTCCC4440.15518018726473948No Hit
GTCTAGATATTGTTTGTCCAAGTCTAAAGTCAATTCCCTTTTCAACATCT4440.15518018726473948No Hit
GTATTTAGTATAGATCTGTTCCTTTTGGTTCTCCAGCTATGAGTTCCAGA4400.15378216755965174No Hit
GAATAAAGGGGCCAAATAAAGAGACAATAAACAGAGAGGTATCAATTTTG4370.15273365278083595No Hit
AAATTACACTGTTGGTTCGGTGGGAAAGAATTTGACCTAGACTCTGCCTT4350.15203464292829208No Hit
CTGATATACAGAAAGCACTAATTGGTGCCTCTATCTGCTTTTTAAAACCC4350.15203464292829208No Hit
GCTTTAGCACTTCCATTACATCCTTTGCAATTCCTTCCCCATTTTTCTGA4320.15098612814947626No Hit
GTCTAGGGGTGTCCAAATAAGTCTTTGTGCACATCAATCTTATTTCCGCT4250.1485395936655727No Hit
ATCCAATGATGACCAATAACCCCATGGACATCTTCGGAGCTTATGTGTAC4230.14784058381302884No Hit
ATGCTACACTGCTTACAAGGTGAAAATTGGAGGCAAATATATCACCCAGG4050.141549495140134No Hit
CCATTAGAAAGATATAATGAAGAAACAAGGGCGAAATTAAAAAGGCTGAA4050.141549495140134No Hit
ACTATATTGTCTCATGGTCATGTACCTGAATCCTGGAAATTATTCAATGC4050.141549495140134No Hit
GATCTATACTAAATACTGATCAGAGGAACATGATTCTTGAAGAACAATGC4010.14015147543504625No Hit
ATACTAAATGGAATGAATGCTTAAATCCACGAATCTTTTTGGCTATGACT4000.13980197050877433No Hit
ACTAAATACTGATCAGAGGAACATGATTCTTGAAGAACAATGCTACGCTA3990.1394524655825024No Hit
TATCTAGACTGAGGGATATATCTGTTCCTGCTGGTTTCTCCAATTTTGAA3890.13595741631978303No Hit
CTGTTAAAACAGGCCTCAAAAAGATTGCAACATTTAGCGTAGCATTGTTC3850.13455939661469526No Hit
GTATAGCACCTGTCTTGTTTTCCAACAAAATAGCCAGATTAGGGAAAGGA3760.13141385227824787No Hit
GTATAGTATGGTTATCCAATCCATCATCATTTAAAGATGCAGCAGTAATG3760.13141385227824787No Hit
CTTATGTATTGTTCCGTTCCCGATTAATATTTCTTCATCATTTTTGAATC3750.13106434735197592No Hit
ATATTGTCTCATGGTCATGTACCTGAATCCTGGAAATTATTCAATGCAAG3730.13036533749943208No Hit
GTTTAATATGCTATCTACCGTGTTGGGAGTAGCAGCACTAGGCATCAAAA3470.12127820941636172No Hit
GTTCATAGCTGAGACCATCTGCATTTCCCGTCTCACTCCTGGCACTGAGG3470.12127820941636172No Hit
GACCTAGACTCTGCCTTGGAATGGATAAAAAACAAAAGATGCTTAACTGA3420.11953068478500203No Hit
GCACTAATTGGTGCCTCTATCTGCTTTTTAAAACCCAAAGACCAGGAAAG3330.11638514044855462No Hit
GTATTTACACAGTCTCTCCAAAGTTTCCATAGGAAATTTTCGTTAGCTTT3310.11568613059601074No Hit
CATTCACACAGAGCTCATAGCAGAGCAGCGAGATCCTCAGTGCCAGGAGT3280.11463761581719495No Hit
TTCTTAAAATGTCGCTGTTTGGAGACACAATTGCCTACCTGCTTTCATTG3280.11463761581719495No Hit
CCCCTAGACCAAATGATGGAAGCATAACAGGGCCTTGCGAATCTGATGGA3260.11393860596465107No Hit
CCCATACACCGGTGTTCCCCCTTATTCCCATGGAACGGGAACAGGCTACA3260.11393860596465107No Hit
AGCACGCACTTTCTTAAAATGTCGCTGTTTGGAGACACAATTGCCTACCT3240.11323959611210721No Hit
GATTATAGGTACACATACAAATGCCACAGAGGAGATTCCAAAGTGGAAGG3200.11184157640701944No Hit
TTATTATTGTCATTCCTATTGCCATATCTGCTGATTCATTTACTCCAGCA3160.1104435567019317No Hit
GTCCTATTGTCCAAGCCATGAAATGGAGAGCTGATAAAATAAAAGAACAA3140.10974454684938785No Hit
GTATGGAACTTCTGGTAGCTCATGGTTGTAAATGTGAGGCCCTATTGGTC3060.10694850743921236No Hit
CATGTATCCAATGATGACCAATAACCCCATGGACATCTTCGGAGCTTATG3010.10520098280785267No Hit
GTTAGTAGCAGATGGTGGGCCCAACATTTACAATTTGAGAAACTTACATA2990.1045019729553088No Hit
GTGTACAATGATAGATCCAACAAATGGGCCATTACCTGAAGACAATGAGC2990.1045019729553088No Hit
GCTATGAACACAGCAAAAACAATGAATGGAATGGGGAAAGGAGAAGACGT2940.10275444832394912No Hit
CCTCTATTCTCTTACAATCCACAAACAGAAGTCCTAACTATATGCGGCAG2910.10170593354513333No Hit
GTCCTGATCTGTTCAGCATACCATTAGAAAGATATAATGAAGAAACAAGG2910.10170593354513333No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATAATT100.007081135145.048612
AGCACGC202.1244323E-6145.048611
GCACAGC100.007081135145.048612
CGGAGCG100.007088541144.997917
GGAGCGT100.007088541144.997918
GCACGCA256.446173E-6116.038892
CACGCAC256.451779E-6116.01863
AAACACG501.8189894E-12115.998325
ACACGAG458.367351E-11112.7761467
AACACGA458.367351E-11112.7761466
CACGTGC203.858057E-4108.786451
GAAACAC553.6379788E-12105.453024
ACGAGCC554.1472958E-1092.271399
TAGAAAC651.4551915E-1189.260682
ACAACGC259.3803887E-486.998748
CAACGCA259.3803887E-486.998749
CACGAGC608.276402E-1084.582118
TGGGAGC950.076.314688
AACAACG300.001934436172.498957
GTCATAA1500.067.689351
Produced by FastQC (version 0.11.5)