FastQCFastQC Report
Tue 2 Jul 2019
HWC5JBCX2_l01_n01_SM2_9567_P1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWC5JBCX2_l01_n01_SM2_9567_P1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2160665
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTTTAAGGCCGTGTTTGAAGAGTCGATAGACACATTTGAAAAAAAGACG181210.8386769813923028No Hit
ACTCTATGCTGACAAAATGACTGTCGTCAGCATCCACAGCATTCTGCTGT121680.5631599530700039No Hit
TTCCAGTACTGGTCTAAGAGATGATCTTCTTGAAAATTTGCAGACCTATC119780.5543663640592132No Hit
TTCCAACACTGTGTCAAGCTTCCAGGTAGATTGCTTTCTTTGGCATATCC112380.5201176489645549No Hit
GTATAGGAAACTTAAGAGGGAGATAACGTTCCACGGGGCCAAAGAAATAG95740.4431043220489988No Hit
GTACTGGAACTAGGATGAGTCCCAATGGCTCTCATTGCCTGCACCATCTG89720.41524252949902No Hit
CAATAGGATGGGGGCTGTAACCACTGAAGTGGCATTTGGCCTGGTGTGTG80100.37071919987596413No Hit
TAGTAATACTATGGTCTCTAGTCGGCCAAAAATCACATTGAAATTCGCTT77090.3567883036009747No Hit
CTCATAGACTCAGGTACTCCTTCCGTAGAAGGGCCTCTTTTAAGGCCGTG75830.35095676562539774No Hit
CTCCAACTCTATGCTGACAAAATGACTGTCGTCAGCATCCACAGCATTCT75800.3508179194831221No Hit
CCTCAATAGTATGTCCTGGAAAAGAAGGCAATGGTGAGATTTCGCCAACA67580.3127740764995962No Hit
TCCTAGTTCCAGTACTGGTCTAAGAGATGATCTTCTTGAAAATTTGCAGA60950.2820890790566793No Hit
TTCCTATACAGTTTAACTGCTTTGTCCATGTTATTTGGGTCTCCATTCCC60510.2800526689699699No Hit
GGTCTAAGAGATGATCTTCTTGAAAATTTGCAGACCTATCAGAAACGAAT59560.27565587446457457No Hit
GTGTTATCATTCCATTCAAGTCCTCCGATGAGGACCCCAATTGCATTTTT59460.2751930539903224No Hit
TTCTTAATCTGTGTCTCACCTCTTCAATTAGCCATCTTATCTCTTCAAAC57890.2679267725445638No Hit
GTATTACTAAGGGCTTTCACCGAAGAGGGAGCAATTGTTGGCGAAATCTC56490.26144728590503385No Hit
CTATTGAGGATGTCAAAAATGCAATTGGGGTCCTCATCGGAGGACTTGAA55890.258670363059521No Hit
TTCTAACCGAGGTCGAAACGTATGTTCTCTCTATCGTTCCATCAGGCCCC53950.2496916458590295No Hit
CTCATGGAATGGCTAAAGACAAGACCAATTCTGTCACCTTTGACTAAGGG52180.2414997234647666No Hit
ATCCTATTGTATATGAGGCCCATGCAACTGGCAAGTGCACCAGCAGAATA49300.228170493806305No Hit
TGTTTGAAGAGTCGATAGACACATTTGAAAAAAAGACGATCAAGAATCCA48490.22442164796486266No Hit
ATATACAATAGGATGGGGGCTGTAACCACTGAAGTGGCATTTGGCCTGGT48090.22257036606785413No Hit
CTGCTGTTCCTTTCGATATTCTTCCCTCATAGACTCAGGTACTCCTTCCG46080.21326767453538611No Hit
GTTGTAATGCTTGCATGAATGTTATTTGTTCAAAGCTATTTTCAGTTGTT45360.2099353671207707No Hit
GCATTCTGCTGTTCCTTTCGATATTCTTCCCTCATAGACTCAGGTACTCC44380.20539972647309973No Hit
GACTTGAAGATGTCTTTGCTGGGAAAAACACAGATCTTGAGGCTCTCATG44170.2044278034771702No Hit
GCACTACAGCTAAGGCTATGGAGCAAATGGCTGGATCAAGTGAGCAGGCA43070.19933677826039667No Hit
GGCCTGGTGTGTGCAACATGTGAGCAGATTGCTGATTCCCAGCACAGGTC42650.19739293226853769No Hit
CTCTTAGACCAGTACTGGAACTAGGATGAGTCCCAATGGCTCTCATTGCC42620.19725408612626208No Hit
AAACAAGGGTGTTTTTTATCATTAAATAAGCTGAAATGAGAAAGTTCTTA41780.19336639414254408No Hit
ATTGTATATGAGGCCCATGCAACTGGCAAGTGCACCAGCAGAATAACTGA41730.193134983905418No Hit
ATACAATAGGATGGGGGCTGTAACCACTGAAGTGGCATTTGGCCTGGTGT39980.18503562560600556No Hit
CAGGTACTCCTTCCGTAGAAGGGCCTCTTTTAAGGCCGTGTTTGAAGAGT39750.18397113851522565No Hit
AAACAAGGTAGTTTTTTACTCCAACTCTATGCTGACAAAATGACTGTCGT39540.18299921551929615No Hit
AGTAGAAACAAGGTAGTTTTTTACTCCAACTCTATGCTGACAAAATGACT38210.1768437032117427No Hit
TAGTATTACTAAGGGCTTTCACCGAAGAGGGAGCAATTGTTGGCGAAATC36760.17013280633508665No Hit
GCCTTAGCTGTAGTGCTGGCCAAAACCATTCTGTTCTCATGTTTTATTAA36410.16851293467520417No Hit
CCCAAATAACATGGACAAAGCAGTTAAACTGTATAGGAAACTTAAGAGGG36130.16721703734729818No Hit
CGTATGTTCTCTCTATCGTTCCATCAGGCCCCCTCAAAGCCGAGATCGCG35890.16610626820909305No Hit
TCTCTCTATCGTTCCATCAGGCCCCCTCAAAGCCGAGATCGCGCAGAGAC35420.16393101198010798No Hit
CCCTAAGGGGAAGAGGCAATACTCTCGGTCTAGACATCAAATCAGCCACC35060.16226485827280027No Hit
GTGTCAAGCTTCCAGGTAGATTGCTTTCTTTGGCATATCCGGAAACAAGT34710.16064498661291776No Hit
CACCAGGCCAAATGCCACTTCAGTGGTTACAGCCCCCATCCTATTGTATA34590.16008960204381523No Hit
GTATATGAGGCCCATGCAACTGGCAAGTGCACCAGCAGAATAACTGAGAG34400.15921024314273616No Hit
GTCGATAGACACATTTGAAAAAAAGACGATCAAGAATCCACAATATCAAG34310.15879370471590923No Hit
GGTAGATATTGAAAGATGAGCCTTCTAACCGAGGTCGAAACGTATGTTCT33900.15689614077147546No Hit
GTAATACTATGGTCTCTAGTCGGCCAAAAATCACATTGAAATTCGCTTTT33790.1563870382497981No Hit
CAGTTATTCTGCTGGTGCACTTGCCAGTTGCATGGGCCTCATATACAATA33760.15624819210752244No Hit
GTTCTTATCTCCTGTTCCACTTCAAACAGTAGTTGTAATGCTTGCATGAA33620.15560024344356946No Hit
CTTAAAATGACCATGGTCTCAACACCTGCTTCGAGATACATAACTGACAT33490.15499857682704168No Hit
CTCAGGTACTCCTTCCGTAGAAGGGCCTCTTTTAAGGCCGTGTTTGAAGA33320.15421178202081304No Hit
GCTAAAGACAAGACCAATTCTGTCACCTTTGACTAAGGGGATTTTAGGGT33190.15361011540428524No Hit
ATGCCCAAGCAGAAAGTGGAAGGACCTCTTTGCATCAGAATGGACCAGGC33160.15347126926200963No Hit
GGCTAAAGACAAGACCAATTCTGTCACCTTTGACTAAGGGGATTTTAGGG33020.15282332059805662No Hit
ATACTATGGTCTCTAGTCGGCCAAAAATCACATTGAAATTCGCTTTTAAC32840.15199024374440276No Hit
ATCCCAATGATATTCGCGGCAACAACAAGCGGGTCACTTGAATCGTTGCA32840.15199024374440276No Hit
CTCCTGTTCCACTTCAAACAGTAGTTGTAATGCTTGCATGAATGTTATTT32200.14902819270918907No Hit
TCCCAGCACAGGTCTCATAGGCAGATGGTGGCAACAACCAATCCATTAAT32180.14893562861433865No Hit
CTATCGTTCCATCAGGCCCCCTCAAAGCCGAGATCGCGCAGAGACTTGAA31980.14800998766583437No Hit
CATTACTGCTTCTCCAAGCGAATCTCTGTAGATTTTTAGAGACTCGAACT31830.14731575695445615No Hit
CCACAATATCAAGTGCAAGATCCCAATGATATTCGCGGCAACAACAAGCG31500.1457884493894241No Hit
ATATACAGGAGAGTCGATGGGAAATGGATGAGGGAACTCGTCCTTTATGA31090.14389088544499032No Hit
TGATAACACAGTTCGAGTCTCTAAAAATCTACAGAGATTCGCTTGGAGAA30960.14328921882846254No Hit
GTCCAAAATGCCCTCAATGGGAATGGAGACCCAAATAACATGGACAAAGC30960.14328921882846254No Hit
ATCAAGTGCAAGATCCCAATGATATTCGCGGCAACAACAAGCGGGTCACT30660.14190075740570612No Hit
GACATAATGGATTCCAACACTGTGTCAAGCTTCCAGGTAGATTGCTTTCT30070.1391701166076185No Hit
TCTGATAGGTCTGCAAATTTTCAAGAAGATCATCTCTTAGACCAGTACTG30050.13907755251276807No Hit
GGTGTGTGCAACATGTGAGCAGATTGCTGATTCCCAGCACAGGTCTCATA29720.13755024494773602No Hit
GTGTCTCACCTCTTCAATTAGCCATCTTATCTCTTCAAACTTCTGACCTA29520.1366246039992317No Hit
GTCTAAGAGATGATCTTCTTGAAAATTTGCAGACCTATCAGAAACGAATG29230.13528242462390053No Hit
CTCATAGGCAGATGGTGGCAACAACCAATCCATTAATAAAACATGAGAAC29090.1346344759599475No Hit
TTGCTAGTCAGGCCAGGCAGATGGTGCAGGCAATGAGAGCCATTGGGACT27530.12741447656161414No Hit
GTAGAAACAAGGGTGTTTTTTATCATTAAATAAGCTGAAATGAGAAAGTT27320.12644255356568462No Hit
GGCAATGAGAGCCATTGGGACTCATCCTAGTTCCAGTACTGGTCTAAGAG27040.12514665623777865No Hit
CTCATATACAATAGGATGGGGGCTGTAACCACTGAAGTGGCATTTGGCCT27030.12510037419035344No Hit
GTTTTTTACTCCAACTCTATGCTGACAAAATGACTGTCGTCAGCATCCAC27030.12510037419035344No Hit
CATGAGAACAGAATGGTTTTGGCCAGCACTACAGCTAAGGCTATGGAGCA26980.12486896395322736No Hit
CCGTAGAAGGGCCTCTTTTAAGGCCGTGTTTGAAGAGTCGATAGACACAT26800.12403588709957351No Hit
GTAGAAACAAGGTAGTTTTTTACTCCAACTCTATGCTGACAAAATGACTG26600.12311024615106923No Hit
CTTATCTCCTGTTCCACTTCAAACAGTAGTTGTAATGCTTGCATGAATGT26180.12116640015921024No Hit
CTCTATCGTTCCATCAGGCCCCCTCAAAGCCGAGATCGCGCAGAGACTTG26150.12102755401693459No Hit
CTATAGGACCTGCAAGTTAGTGGGAATCAACATGAGCAAAAAGAAGTCCT26090.1207498617323833No Hit
TCTTAGACCAGTACTGGAACTAGGATGAGTCCCAATGGCTCTCATTGCCT26050.12056473354268246No Hit
CCATAGTATTACTAAGGGCTTTCACCGAAGAGGGAGCAATTGTTGGCGAA25860.11968537464160339No Hit
CTGTAGTGCTGGCCAAAACCATTCTGTTCTCATGTTTTATTAATGGATTG25840.11959281054675297No Hit
CCCTAGAAGGTCCGGGGCTGCAGGTGCTGCAGTCAAAGGAATCGGAACAA25750.11917627211992603No Hit
GCCTATGAGACCTGTGCTGGGAATCAGCAATCTGCTCACATGTTGCACAC25510.11806550298172092No Hit
GCAGTTAAACTGTATAGGAAACTTAAGAGGGAGATAACGTTCCACGGGGC25390.11751011841261833No Hit
CCATTGGGACTCATCCTAGTTCCAGTACTGGTCTAAGAGATGATCTTCTT25230.11676960565381492No Hit
ATAGTATGTCCTGGAAAAGAAGGCAATGGTGAGATTTCGCCAACAATTGC24680.11422409304542815No Hit
GGTCTCATAGGCAGATGGTGGCAACAACCAATCCATTAATAAAACATGAG24660.11413152895057771No Hit
ATACATAACTGACATGACTATTGAAGAATTGTCAAGAAACTGGTTCATGC24650.1140852469031525No Hit
CAAAATGACTGTCGTCAGCATCCACAGCATTCTGCTGTTCCTTTCGATAT24420.11302075981237257No Hit
GTTCTCATGTTTTATTAATGGATTGGTTGTTGCCACCATCTGCCTATGAG24380.11283563162267171No Hit
GTAGTTGTAATGCTTGCATGAATGTTATTTGTTCAAAGCTATTTTCAGTT24360.11274306752782129No Hit
CTTCTGCATTGTCTCCGAAGAAATAAGATCCTTCGTTACTCATATCAAAA24240.11218768295871873No Hit
GTACTGGTCTAAGAGATGATCTTCTTGAAAATTTGCAGACCTATCAGAAA24160.11181742657931702No Hit
CTATTGAAGAATTGTCAAGAAACTGGTTCATGCTAATGCCCAAGCAGAAA24110.11158601634219095No Hit
CCCCCATTCGTTTCTGATAGGTCTGCAAATTTTCAAGAAGATCATCTCTT24020.11116947791536401No Hit
CAATGGGAATGGAGACCCAAATAACATGGACAAAGCAGTTAAACTGTATA23830.11029011901428495No Hit
GTTCCAGTACTGGTCTAAGAGATGATCTTCTTGAAAATTTGCAGACCTAT23690.10964217035033196No Hit
GACCAATTCTGTCACCTTTGACTAAGGGGATTTTAGGGTTTGTTTTCACG23650.10945704216063111No Hit
GAATAACTGAGAGCTATTTCTTTGGCCCCGTGGAACGTTATCTCCCTCTT23630.10936447806578067No Hit
GTGCTGGGAATCAGCAATCTGCTCACATGTTGCACACACCAGGCCAAATG23590.10917934987607983No Hit
GTCTAGACATCAAATCAGCCACCCATGTTGGAAAGCAAATTGTAGAAAAG23480.10867024735440246No Hit
GTGCAACATGTGAGCAGATTGCTGATTCCCAGCACAGGTCTCATAGGCAG23220.1074669141213469No Hit
GACTCAGGTACTCCTTCCGTAGAAGGGCCTCTTTTAAGGCCGTGTTTGAA23190.10732806797907127No Hit
CATAGACTCAGGTACTCCTTCCGTAGAAGGGCCTCTTTTAAGGCCGTGTT23160.10718922183679562No Hit
AACATGGACAAAGCAGTTAAACTGTATAGGAAACTTAAGAGGGAGATAAC23050.10668011931511825No Hit
CTCAATATCAGTGCAGATCTTGCTAAAAATATGAGATCTTCGATCTCAGC22970.10630986293571655No Hit
GTAGAAGGGCCTCTTTTAAGGCCGTGTTTGAAGAGTCGATAGACACATTT22890.10593960655631485No Hit
CATATACAATAGGATGGGGGCTGTAACCACTGAAGTGGCATTTGGCCTGG22880.10589332450888962No Hit
GTTTTGGCCAGCACTACAGCTAAGGCTATGGAGCAAATGGCTGGATCAAG22810.10556935017691313No Hit
CCTATATGAACTACTAGGGAAAAATTTCTCGAACAAGTTGCAGCACTTCT22700.10506024765523578No Hit
GTGCAGGCAATGAGAGCCATTGGGACTCATCCTAGTTCCAGTACTGGTCT22550.10436601694385757No Hit
GGACAAAGCAGTTAAACTGTATAGGAAACTTAAGAGGGAGATAACGTTCC22430.103810632374755No Hit
CCTTTGACTAAGGGGATTTTAGGGTTTGTTTTCACGCTCACCGTGCCCAG22360.1034866580427785No Hit
TATCAAAAGAGGGCACGATCGGGTTCGCTGCCTTCTCGTCTGAGAGCTCG22280.1031164016633768No Hit
GATCTTGAGGCTCTCATGGAATGGCTAAAGACAAGACCAATTCTGTCACC22280.1031164016633768No Hit
CCTCAAAGCCGAGATCGCGCAGAGACTTGAAGATGTCTTTGCTGGGAAAA22160.1025610170942742No Hit
CCTATCAGAAACGAATGGGGGTGCAGATGCAACGATTCAAGTGACCCGCT21930.10149653000349429No Hit
TTATTTGGGTCTCCATTCCCATTGAGGGCATTTTGGACAAAGCGTCTACG21670.10029319677043871No Hit
GCTAAGGCTATGGAGCAAATGGCTGGATCAAGTGAGCAGGCAGCGGAGGC21610.10001550448588745No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAGTACG1950.089.229389
GCGAAAG7800.079.9552
CGAAAGC7850.079.445743
TCACGCC406.664688E-572.50055145
AGGGTGT10350.066.55416
AGCGAAA9950.062.6871
ACAGTAC2900.059.999068
GAGCCGA1600.058.913516
GTATACG502.0070169E-458.021921
TATACGT502.0083952E-458.0138632
GGAGCCG1950.055.7799725
GCTTATA1600.054.395551
CTATAGG7600.053.441241
ATACCGG553.2200883E-452.7264487
GGTGTTT13100.052.5755128
TAGAAAC18900.051.798092
TTAACCA701.6947128E-551.7956964
TTGAGTC1700.051.175679
GGACCGC1004.7020876E-850.7562566
TAGAACC1750.049.7238664
Produced by FastQC (version 0.11.5)